BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0459.0037.p (432 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B0TIB5.1 B0TIB5_HELMI Glutamate synthase (Nadph) small chain 620 e-176 A4J8A5.1 A4J8A5_DESRM Glutamate synthase (NADH) small subunit EC... 613 e-173 A5D4B5.1 A5D4B5_PELTS NADPH-dependent glutamate synthase beta ch... 602 e-170 B9XFF1.1 B9XFF1_9BACT Glutamate synthase, NADH/NADPH, small subunit 592 e-167 B4VIZ3.1 B4VIZ3_9CYAN Glutamate synthase, NADH/NADPH, small subu... 585 e-165 >B0TIB5.1 B0TIB5_HELMI Glutamate synthase (Nadph) small chain Length = 494 Score = 620 bits (1600), Expect = e-176, Method: Compositional matrix adjust. Identities = 291/431 (67%), Positives = 349/431 (80%) Query: 1 MASGCPLHNLIPEWNDMVYRGNWEVALKRLHKTNNFPEFTGRVCPAPCEGACTVGMNGKP 60 M SGCPLHNL PEWND+VYRG W+ A +RL KT+NFPEFTGRVCP+PCEGACT G+ P Sbjct: 63 MVSGCPLHNLTPEWNDLVYRGLWKEAWRRLKKTSNFPEFTGRVCPSPCEGACTAGLATSP 122 Query: 61 VTIKSIEKAIVDRGFEEGWIVPQPPLTRTGKKVAIVGSGPAGLACAAQLNKAGHSVTVYE 120 VTIK+IE I+D+ FEEGW VP P RTGKKVA++GSGP+GLA A QLNKAGHSVTV+E Sbjct: 123 VTIKNIECHIIDKAFEEGWEVPTEPAKRTGKKVAVIGSGPSGLAAADQLNKAGHSVTVFE 182 Query: 121 RADRIGGLLTYGIPNMKLDKKTVQRRVDLLAAEGITFVTRTEIGRDIPASQLKEEHDAVV 180 RADRIGGLL YGIPNMKLDK VQRRVDL+AA GITF+T E+G+D PA +LKEE DAVV Sbjct: 183 RADRIGGLLMYGIPNMKLDKSIVQRRVDLMAASGITFITNCEVGKDYPADKLKEEFDAVV 242 Query: 181 LCGGSTQARDLALEGRGLRGIHQAMEFLTLNTKSLLDSGLADGEYISAAXXXXXXXXXXX 240 LCGGST+ RDL +EGR L+G+H AMEFL NTKSLLDS L DG +ISA Sbjct: 243 LCGGSTKPRDLPVEGRNLKGVHFAMEFLAANTKSLLDSNLTDGNFISAEGKDVIVIGGGD 302 Query: 241 XXXXCVATSIRHGCRSVIQLEIMPQSPLTRQAGNPWPEWPKVLKVDYGQQEAASLYHEDP 300 CV T++RH C SV Q+EIMPQ P+ R A NPWP++P+VLK+DYGQ+EAA++ DP Sbjct: 303 TGTDCVGTALRHRCNSVYQIEIMPQPPVERAADNPWPQFPRVLKIDYGQEEAAAIAGADP 362 Query: 301 RRYLVNTKRFVGDDGGHVQELHTVRIEWSRNEQGRMVPVEVPGSEEVIKAQLVLLALGFT 360 R YL++TK+FVGD+ GHV+E+HTV+IEW ++EQGRM P E+ G+E+V AQLVLLA+GF Sbjct: 363 RHYLISTKKFVGDEKGHVKEVHTVQIEWVKDEQGRMTPKEIAGTEKVWPAQLVLLAMGFL 422 Query: 361 GPEETVLGQLGVERDERSNAKAEFGAQATNVEGVFAAGDIRRGQSLVVWAINEGRQAARE 420 GPE+ +L QLG+ERD RSN KA+FG ATNV G+FAAGD+RRGQSLVVWAI+EGRQAARE Sbjct: 423 GPEDQLLTQLGIERDPRSNVKADFGKFATNVPGIFAAGDMRRGQSLVVWAISEGRQAARE 482 Query: 421 VDRFLTGSSNL 431 VD++L G++NL Sbjct: 483 VDKYLMGTTNL 493 >A4J8A5.1 A4J8A5_DESRM Glutamate synthase (NADH) small subunit EC=1.4.1.14 Length = 490 Score = 613 bits (1580), Expect = e-173, Method: Compositional matrix adjust. Identities = 290/429 (67%), Positives = 351/429 (81%), Gaps = 1/429 (0%) Query: 1 MASGCPLHNLIPEWNDMVYRGNWEVALKRLHKTNNFPEFTGRVCPAPCEGACTVGMNGKP 60 MASGCP HN+ PEWND+VYRG W+ A RL KTNNFPEFTGRVCPA CEGACT G+ G P Sbjct: 63 MASGCPNHNVPPEWNDLVYRGLWKEAYLRLIKTNNFPEFTGRVCPALCEGACTAGLIGSP 122 Query: 61 VTIKSIEKAIVDRGFEEGWIVPQPPLTRTGKKVAIVGSGPAGLACAAQLNKAGHSVTVYE 120 VT+KSIE AI+DR F EGW++PQPP TRTGK+VA++GSGPAGLACAA+LNK+GH VTV+E Sbjct: 123 VTVKSIECAIIDRAFAEGWVLPQPPATRTGKRVAVIGSGPAGLACAAELNKSGHWVTVFE 182 Query: 121 RADRIGGLLTYGIPNMKLDKKTVQRRVDLLAAEGITFVTRTEIGRDIPASQLKEEHDAVV 180 RADRIGGLL YGIPNMKLDKK VQRRV+LLAAEGI F+T+TE+G+D P +L + DAVV Sbjct: 183 RADRIGGLLMYGIPNMKLDKKAVQRRVELLAAEGIKFITKTEVGKDYPTDELLKNFDAVV 242 Query: 181 LCGGSTQARDLALEGRGLRGIHQAMEFLTLNTKSLLDSGLADGEYISAAXXXXXXXXXXX 240 LCGG+T+ RDL +EGR L+G+H A+EFL++NTKSLLDS DG +ISA Sbjct: 243 LCGGATKPRDLPVEGRNLQGVHFAVEFLSINTKSLLDSQHKDGNFISAQGKDVLVIGGGD 302 Query: 241 XXXXCVATSIRHGCRSVIQLEIMPQSPLTRQAGNPWPEWPKVLKVDYGQQEAASLYHEDP 300 CVAT++RHGC+SV+QLE+MPQ P R NPWPE+P+VLKVDYGQQEAA+LY EDP Sbjct: 303 TGTDCVATALRHGCKSVVQLELMPQLPKERTTNNPWPEYPRVLKVDYGQQEAAALYGEDP 362 Query: 301 RRYLVNTKRFVGDDGGHVQELHTVRIEWSRNEQGRMVPVEVPGSEEVIKAQLVLLALGFT 360 RRY V TK+ VGD G V+E+HTV++EW NE+GR+VP E+ GS+++ AQLVLLA+GF Sbjct: 363 RRYCVLTKKIVGDAQGQVKEVHTVQVEWV-NEEGRLVPKEIAGSQKIWPAQLVLLAMGFV 421 Query: 361 GPEETVLGQLGVERDERSNAKAEFGAQATNVEGVFAAGDIRRGQSLVVWAINEGRQAARE 420 GP++++L QLGV+RDERSNAKAE+G TNV+GVFAAGD+RRGQSLV+WAINEGR AAR Sbjct: 422 GPDDSILDQLGVDRDERSNAKAEYGMFTTNVKGVFAAGDMRRGQSLVIWAINEGRGAARA 481 Query: 421 VDRFLTGSS 429 DR+L G + Sbjct: 482 CDRYLMGDN 490 >A5D4B5.1 A5D4B5_PELTS NADPH-dependent glutamate synthase beta chain and related oxidoreductases Length = 494 Score = 602 bits (1553), Expect = e-170, Method: Compositional matrix adjust. Identities = 291/429 (67%), Positives = 342/429 (79%) Query: 1 MASGCPLHNLIPEWNDMVYRGNWEVALKRLHKTNNFPEFTGRVCPAPCEGACTVGMNGKP 60 M SGCP HNLIPEWND+VYRG W+ AL RL KTN+FPEFTGRVCPA CEGAC G+ P Sbjct: 63 MISGCPNHNLIPEWNDLVYRGLWKEALNRLLKTNSFPEFTGRVCPALCEGACAAGLYSAP 122 Query: 61 VTIKSIEKAIVDRGFEEGWIVPQPPLTRTGKKVAIVGSGPAGLACAAQLNKAGHSVTVYE 120 V KSIEKAI++R +EEGW+VP+PP RTGKKVA+VGSGPAGLACA LNKAGH VTV+E Sbjct: 123 VAAKSIEKAIIERAYEEGWMVPKPPARRTGKKVAVVGSGPAGLACADWLNKAGHLVTVFE 182 Query: 121 RADRIGGLLTYGIPNMKLDKKTVQRRVDLLAAEGITFVTRTEIGRDIPASQLKEEHDAVV 180 RADR GGLL YGIPNMKLDKK V+RR+DL+A EG+TF+T TE+GRD PA +L +E DAVV Sbjct: 183 RADRPGGLLMYGIPNMKLDKKIVRRRIDLMAGEGVTFITSTEVGRDYPAGRLLKEFDAVV 242 Query: 181 LCGGSTQARDLALEGRGLRGIHQAMEFLTLNTKSLLDSGLADGEYISAAXXXXXXXXXXX 240 LCGGST+ RDL +EGRGL+GIH A+EFL NTKSLLDSGL DG++ISAA Sbjct: 243 LCGGSTRPRDLPIEGRGLKGIHFAVEFLGANTKSLLDSGLKDGKFISAAGKDVIVIGGGD 302 Query: 241 XXXXCVATSIRHGCRSVIQLEIMPQSPLTRQAGNPWPEWPKVLKVDYGQQEAASLYHEDP 300 CVATSIRH CRSV QLEIMP+ PL R NPWP++P+VLKVDYGQ+EA +L+ DP Sbjct: 303 TGTDCVATSIRHKCRSVHQLEIMPRPPLGRAPDNPWPQFPRVLKVDYGQEEAKALFGADP 362 Query: 301 RRYLVNTKRFVGDDGGHVQELHTVRIEWSRNEQGRMVPVEVPGSEEVIKAQLVLLALGFT 360 R Y + K+F+GD+ G V+ + TV +EW + QGR+VP E+PGS +V AQLVLLA+GF Sbjct: 363 RHYCITAKKFIGDECGRVKGVFTVNVEWVADGQGRLVPREIPGSGKVWPAQLVLLAMGFA 422 Query: 361 GPEETVLGQLGVERDERSNAKAEFGAQATNVEGVFAAGDIRRGQSLVVWAINEGRQAARE 420 GPEE +L QLGVERDER+NAKAE+G ATNV GVF AGD+RRGQSLV+WAINEGR AARE Sbjct: 423 GPEEGLLEQLGVERDERTNAKAEYGVFATNVRGVFVAGDMRRGQSLVIWAINEGRGAARE 482 Query: 421 VDRFLTGSS 429 DR+L G + Sbjct: 483 CDRYLKGYA 491 >B9XFF1.1 B9XFF1_9BACT Glutamate synthase, NADH/NADPH, small subunit Length = 493 Score = 592 bits (1527), Expect = e-167, Method: Compositional matrix adjust. Identities = 279/432 (64%), Positives = 343/432 (79%), Gaps = 1/432 (0%) Query: 1 MASGCPLHNLIPEWNDMVYRGNWEVALKRLHKTNNFPEFTGRVCPAPCEGACTVGMNGKP 60 MASGCP++NLIPEWND+VYRG W+ AL RLHKTNNFPEFTGRVCPAPCEG+C +G++ P Sbjct: 63 MASGCPINNLIPEWNDLVYRGLWKEALDRLHKTNNFPEFTGRVCPAPCEGSCVLGIHSPP 122 Query: 61 VTIKSIEKAIVDRGFEEGWIVPQPPLTRTGKKVAIVGSGPAGLACAAQLNKAGHSVTVYE 120 VTIK+IE +I+D+G+EEGW+ +PP RTGKKVA++GSGPAGL AAQLN+AGH VTV+E Sbjct: 123 VTIKNIECSIIDKGWEEGWVTAEPPAMRTGKKVAVIGSGPAGLCAAAQLNRAGHWVTVFE 182 Query: 121 RADRIGGLLTYGIPNMKLDKKTVQRRVDLLAAEGITFVTRTEIGRDIPASQLKEEHDAVV 180 RADR+GGLL YGIPNMKLDK VQRRVDL+A EGITF T TE+G++ P +L +E DAVV Sbjct: 183 RADRVGGLLMYGIPNMKLDKDVVQRRVDLMAKEGITFTTGTEVGKNYPTEKLMQEFDAVV 242 Query: 181 LCGGSTQARDLALEGRGLRGIHQAMEFLTLNTKSLLDSGLADGEYISAAXXXXXXXXXXX 240 +C G+T+ RDL +EGR L+GIH AMEFL NTKSLLD +G +ISA Sbjct: 243 ICTGATKPRDLPIEGRQLKGIHFAMEFLHNNTKSLLDKH-RNGSFISAEGKDVVVIGGGD 301 Query: 241 XXXXCVATSIRHGCRSVIQLEIMPQSPLTRQAGNPWPEWPKVLKVDYGQQEAASLYHEDP 300 CV TS+RHGC+S++QLEI+PQ P+ R NPWPEWPKV ++DYGQ+EAA+ + DP Sbjct: 302 TGTDCVGTSLRHGCKSLVQLEILPQPPVERAKDNPWPEWPKVYRLDYGQEEAAAKFGADP 361 Query: 301 RRYLVNTKRFVGDDGGHVQELHTVRIEWSRNEQGRMVPVEVPGSEEVIKAQLVLLALGFT 360 R YL + +FVGD+ GHV+E+HT+ I W +NE+G+ VP V G+E+V AQ+VLLA+GF Sbjct: 362 RTYLTTSTKFVGDEQGHVKEVHTIEIRWDKNEKGQFVPQPVAGTEKVRPAQVVLLAMGFL 421 Query: 361 GPEETVLGQLGVERDERSNAKAEFGAQATNVEGVFAAGDIRRGQSLVVWAINEGRQAARE 420 GPE+ +L QLGVERD RSNAKA++G +TNV+GVFAAGD RRGQSLVVWA NEGR AARE Sbjct: 422 GPEQPLLEQLGVERDGRSNAKADYGRYSTNVKGVFAAGDARRGQSLVVWAFNEGRGAARE 481 Query: 421 VDRFLTGSSNLP 432 DRFL G +NLP Sbjct: 482 CDRFLMGRTNLP 493 >B4VIZ3.1 B4VIZ3_9CYAN Glutamate synthase, NADH/NADPH, small subunit subfamily Length = 495 Score = 585 bits (1507), Expect = e-165, Method: Compositional matrix adjust. Identities = 274/433 (63%), Positives = 337/433 (77%), Gaps = 2/433 (0%) Query: 1 MASGCPLHNLIPEWNDMVYRGNWEVALKRLHKTNNFPEFTGRVCPAPCEGACTVGMNGKP 60 MASGCP++NLIPEWND++YRG W AL RLHKTNNFPEFTGRVCPAPCEG+C +G++ P Sbjct: 63 MASGCPINNLIPEWNDLIYRGRWREALDRLHKTNNFPEFTGRVCPAPCEGSCVLGIHNPP 122 Query: 61 VTIKSIEKAIVDRGFEEGWIVPQPPLTRTGKKVAIVGSGPAGLACAAQLNKAGHSVTVYE 120 VTIK+IE +I+D+G+EEGW+ P+PP RTGKKVAI+GSGPAGL AAQLNKAGH +TV+E Sbjct: 123 VTIKNIECSIIDKGWEEGWVEPEPPTKRTGKKVAIIGSGPAGLCAAAQLNKAGHWITVFE 182 Query: 121 RADRIGGLLTYGIPNMKLDKK-TVQRRVDLLAAEGITFVTRTEIGRDIPASQLKEEHDAV 179 R DR GGLL YGIPNMKLDKK VQRR+D L EG+ F+ TE+G+D P L +E DAV Sbjct: 183 REDRPGGLLMYGIPNMKLDKKQVVQRRLDQLEQEGVKFICNTEVGKDFPTENLLKEFDAV 242 Query: 180 VLCGGSTQARDLALEGRGLRGIHQAMEFLTLNTKSLLDSGLADGEYISAAXXXXXXXXXX 239 VLC G+T+ RDL +EGR L+GIH AM+FLT NTK+LLD+ +G +ISA Sbjct: 243 VLCTGATKPRDLPIEGRDLKGIHFAMDFLTANTKALLDNS-KNGNFISAEGKDVVIIGGG 301 Query: 240 XXXXXCVATSIRHGCRSVIQLEIMPQSPLTRQAGNPWPEWPKVLKVDYGQQEAASLYHED 299 CV TS+RHGC+++ QLEIMPQ PL R NPWPEWPK+ ++DYGQ+EAA+ + D Sbjct: 302 DTGTDCVGTSLRHGCKTLAQLEIMPQPPLERAPDNPWPEWPKIYRMDYGQEEAAAKFGAD 361 Query: 300 PRRYLVNTKRFVGDDGGHVQELHTVRIEWSRNEQGRMVPVEVPGSEEVIKAQLVLLALGF 359 PR YL +F GD+ GHV+ +HTV IEW +NEQGR +P VPG+E+VI AQLVLLA+GF Sbjct: 362 PRSYLTTATKFEGDEKGHVKAVHTVTIEWQKNEQGRFIPSPVPGTEKVIPAQLVLLAMGF 421 Query: 360 TGPEETVLGQLGVERDERSNAKAEFGAQATNVEGVFAAGDIRRGQSLVVWAINEGRQAAR 419 GPE+ +L LG+ER+ RSN KA++G AT++ GVFAAGD RRGQSLVVWA NEGR AAR Sbjct: 422 LGPEQPLLDALGLERNARSNIKADYGKYATSIPGVFAAGDCRRGQSLVVWAFNEGRGAAR 481 Query: 420 EVDRFLTGSSNLP 432 E DR+L GS++LP Sbjct: 482 ECDRYLMGSTDLP 494 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.135 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,525,331,229 Number of extensions: 104012117 Number of successful extensions: 332622 Number of sequences better than 1.0e-05: 2572 Number of HSP's gapped: 326909 Number of HSP's successfully gapped: 2918 Length of query: 432 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 293 Effective length of database: 1,751,175,118 Effective search space: 513094309574 Effective search space used: 513094309574 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)