BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0459.0033.p (181 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D929.1 B1D929_9BACL N-carbamoylputrescine amidase 280 3e-74 A6LUR1.1 A6LUR1_CLOB8 Nitrilase/cyanide hydratase and apolipopro... 273 6e-72 B0THU3.1 B0THU3_HELMI Hydrolase, carbon-nitrogen family, putative 249 5e-65 Q87UB1.1 Q87UB1_PSESM Carbon-nitrogen hydrolase family protein 239 1e-61 Q4ZLL2.1 Q4ZLL2_PSEU2 Nitrilase/cyanide hydratase and apolipopro... 238 2e-61 >B1D929.1 B1D929_9BACL N-carbamoylputrescine amidase Length = 292 Score = 280 bits (717), Expect = 3e-74, Method: Compositional matrix adjust. Identities = 132/165 (80%), Positives = 141/165 (85%) Query: 1 MSCSGDIDENIRKAETLVRXXXXXXXXXXXXXXXFETPYFCQKEKSDYYAYATELEHNKA 60 MSC+ +I+ENIRKAE LVR FETPYFCQKEK+DYY YATELE NKA Sbjct: 11 MSCTSNIEENIRKAEALVREAAAQGAQIILLQELFETPYFCQKEKADYYVYATELEENKA 70 Query: 61 VNHFQAIAKELQVVLPISFYEKKNYARYNSLAVIDADGTVLGKYRKSHIPDGPGYEEKFY 120 VNHF+ IAKEL VVLPISFYEKKN+ARYNSLAVIDADG VLGKYRKSHIPDGPGYEEKFY Sbjct: 71 VNHFRGIAKELGVVLPISFYEKKNWARYNSLAVIDADGEVLGKYRKSHIPDGPGYEEKFY 130 Query: 121 FNPGDTGFKVWNTRYAKIGVGVCWDQWYPEAARVMSLMGAEILFY 165 FNPGDTGFKVW T+YAKIGVGVCWDQWYPEAAR M+LMGAE+LFY Sbjct: 131 FNPGDTGFKVWKTKYAKIGVGVCWDQWYPEAARSMALMGAELLFY 175 >A6LUR1.1 A6LUR1_CLOB8 Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase Length = 292 Score = 273 bits (697), Expect = 6e-72, Method: Compositional matrix adjust. Identities = 124/165 (75%), Positives = 138/165 (83%) Query: 1 MSCSGDIDENIRKAETLVRXXXXXXXXXXXXXXXFETPYFCQKEKSDYYAYATELEHNKA 60 MSCS +IDENI KAE VR FETPYFCQKEKSDYY YATE+ NKA Sbjct: 11 MSCSCNIDENISKAEKFVREAAGKGAQIILLQELFETPYFCQKEKSDYYVYATEVAQNKA 70 Query: 61 VNHFQAIAKELQVVLPISFYEKKNYARYNSLAVIDADGTVLGKYRKSHIPDGPGYEEKFY 120 +NHF+ IAKEL+VVLPISFYEKKNYARYN++A+IDA+G +LG YRKSHIPDGPGYEEKFY Sbjct: 71 INHFKEIAKELKVVLPISFYEKKNYARYNAIAIIDANGEILGTYRKSHIPDGPGYEEKFY 130 Query: 121 FNPGDTGFKVWNTRYAKIGVGVCWDQWYPEAARVMSLMGAEILFY 165 FNPGDTGFKVWNTRY KIGVG+CWDQWYPEAAR M+LMGAE++FY Sbjct: 131 FNPGDTGFKVWNTRYGKIGVGICWDQWYPEAARCMTLMGAEMIFY 175 >B0THU3.1 B0THU3_HELMI Hydrolase, carbon-nitrogen family, putative Length = 295 Score = 249 bits (637), Expect = 5e-65, Method: Compositional matrix adjust. Identities = 115/165 (69%), Positives = 129/165 (78%) Query: 1 MSCSGDIDENIRKAETLVRXXXXXXXXXXXXXXXFETPYFCQKEKSDYYAYATELEHNKA 60 MSCS D+D NI KAE LVR FE PYFCQ E+ ++Y ATE E+N A Sbjct: 11 MSCSWDVDANIAKAEKLVRKAARQGAQVILLQELFEAPYFCQTERPEHYDLATETENNSA 70 Query: 61 VNHFQAIAKELQVVLPISFYEKKNYARYNSLAVIDADGTVLGKYRKSHIPDGPGYEEKFY 120 V HFQ IAKEL VVLPISF+EKKN ARYNS+A+IDADG +LG YRK+HIPDGPGYEEKFY Sbjct: 71 VRHFQPIAKELGVVLPISFFEKKNNARYNSIAMIDADGEILGVYRKTHIPDGPGYEEKFY 130 Query: 121 FNPGDTGFKVWNTRYAKIGVGVCWDQWYPEAARVMSLMGAEILFY 165 FNPGDTGF+VW TRY KIGVG+CWDQW+PEAAR M+LMGAEIL Y Sbjct: 131 FNPGDTGFQVWTTRYGKIGVGICWDQWFPEAARCMALMGAEILLY 175 >Q87UB1.1 Q87UB1_PSESM Carbon-nitrogen hydrolase family protein Length = 292 Score = 239 bits (609), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 110/165 (66%), Positives = 124/165 (75%) Query: 1 MSCSGDIDENIRKAETLVRXXXXXXXXXXXXXXXFETPYFCQKEKSDYYAYATELEHNKA 60 M+CS D++ NI AE LVR FETPYFCQK +DY AT LE N A Sbjct: 12 MACSWDLEANIETAEKLVREAAAKGAQIILIQELFETPYFCQKPNADYLQLATTLESNVA 71 Query: 61 VNHFQAIAKELQVVLPISFYEKKNYARYNSLAVIDADGTVLGKYRKSHIPDGPGYEEKFY 120 + HFQ IAKELQVVLPISF+E AR+N++A+IDADGT LG YRKSHIPDGPGY EK+Y Sbjct: 72 IKHFQKIAKELQVVLPISFFELAGRARFNTIAIIDADGTNLGIYRKSHIPDGPGYHEKYY 131 Query: 121 FNPGDTGFKVWNTRYAKIGVGVCWDQWYPEAARVMSLMGAEILFY 165 FNPGDTGFKVW TRYAKIGVG+CWDQW+PE AR M+L GAEILFY Sbjct: 132 FNPGDTGFKVWQTRYAKIGVGICWDQWFPECARSMALQGAEILFY 176 >Q4ZLL2.1 Q4ZLL2_PSEU2 Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase Length = 292 Score = 238 bits (606), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 110/165 (66%), Positives = 123/165 (74%) Query: 1 MSCSGDIDENIRKAETLVRXXXXXXXXXXXXXXXFETPYFCQKEKSDYYAYATELEHNKA 60 M+CS D++ NI AE LVR FETPYFCQK DY AT LE N A Sbjct: 12 MACSWDLEANIETAEKLVREAAAKGAQIILIQELFETPYFCQKPNPDYLQLATTLESNVA 71 Query: 61 VNHFQAIAKELQVVLPISFYEKKNYARYNSLAVIDADGTVLGKYRKSHIPDGPGYEEKFY 120 + HFQ IAKELQVVLPISF+E AR+NS+A+IDADG+ LG YRKSHIPDGPGY EK+Y Sbjct: 72 IKHFQKIAKELQVVLPISFFELAGRARFNSIAIIDADGSNLGIYRKSHIPDGPGYHEKYY 131 Query: 121 FNPGDTGFKVWNTRYAKIGVGVCWDQWYPEAARVMSLMGAEILFY 165 FNPGDTGFKVW TRYAKIGVG+CWDQW+PE AR M+L GAEILFY Sbjct: 132 FNPGDTGFKVWQTRYAKIGVGICWDQWFPECARSMALQGAEILFY 176 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.138 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,087,699,993 Number of extensions: 44404109 Number of successful extensions: 83936 Number of sequences better than 1.0e-05: 810 Number of HSP's gapped: 84228 Number of HSP's successfully gapped: 810 Length of query: 181 Length of database: 3,060,696,203 Length adjustment: 127 Effective length of query: 54 Effective length of database: 1,864,227,298 Effective search space: 100668274092 Effective search space used: 100668274092 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)