BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0459.0031.p (271 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q5KU98.1 Q5KU98_GEOKA Amino acid ABC transporter (Substrate-bind... 246 1e-63 B7GMP3.1 B7GMP3_ANOFW ABC-type glutamine transport system, perip... 244 4e-63 B4BPF0.1 B4BPF0_9BACI Extracellular solute-binding protein family 3 241 4e-62 A4ITR8.1 A4ITR8_GEOTN Glutamine transport system substrate-bindi... 241 7e-62 C3IZE0.1 C3IZE0_9BACI Extracellular solute-binding protein family 3 238 5e-61 >Q5KU98.1 Q5KU98_GEOKA Amino acid ABC transporter (Substrate-binding protein) Length = 273 Score = 246 bits (629), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 124/267 (46%), Positives = 177/267 (66%), Gaps = 6/267 (2%) Query: 5 KKWAMSAFVALLVLAIAXXXXXXXXXXXXXX----XETIKFASDASYAPMEYMDTDKIKG 60 KK + A VA L +A+A + I +DA++AP EYM KI G Sbjct: 5 KKGLIVAVVAALFMALAACGKSTETSSPSGAEKEGKQKITVGTDAAFAPFEYMQKGKIVG 64 Query: 61 FDIDFIKAVMEEAGIDYTVTNTGWDTMLSSVKQGTEYQAGLSSVSITDERKETYDYSIPY 120 FD D + AVM+EAG+DY + N GWD + +S+ Q E G+S ++ITDERK++YD+S PY Sbjct: 65 FDADLLDAVMKEAGLDYELKNIGWDPLFASL-QSKEIDMGISGITITDERKQSYDFSAPY 123 Query: 121 FESTNMIMVKEGSDIKNALDLKDKKVAVQAATTADDLMSGIMGVDNGNLKRFDSNAVALM 180 FE+T +I+VK+GS +KNALDLK K + VQ ATT + + G + ++K+F+++ VA+M Sbjct: 124 FEATQVILVKQGSPVKNALDLKGKTIGVQNATTGQEAAEKLFGKGD-HIKKFETSVVAIM 182 Query: 181 ELNSGGXXXXXXXXXXXNEYIKNNPNEKLTGIIDKENFGSEYYGILYPKGSEWKAKLDPA 240 EL +GG NEY+KNNP++KL I D +NF SEYYG+++PK SE KAK+D A Sbjct: 183 ELLNGGVDAVITDNAVANEYVKNNPDKKLQVIEDPQNFASEYYGMIFPKDSELKAKVDEA 242 Query: 241 IKKIIENGKYAEIYKTWFGEEPDTQTL 267 ++K+I++GKYAEIYK WFG+EP+ + L Sbjct: 243 LQKVIDSGKYAEIYKKWFGKEPNMEGL 269 >B7GMP3.1 B7GMP3_ANOFW ABC-type glutamine transport system, periplasmic-binding component Length = 265 Score = 244 bits (624), Expect = 4e-63, Method: Compositional matrix adjust. Identities = 118/263 (44%), Positives = 175/263 (66%), Gaps = 3/263 (1%) Query: 5 KKWAMSAFVALLVLAIAXXXXXXXXXXXXXXXETIKFASDASYAPMEYMDTDKIKGFDID 64 KK ++ V +L+ A+ T+ +DAS+AP EYM+ +I GFD+D Sbjct: 2 KKGIIATLVFMLMFALVGCGSSTEKTATTEKKITV--VTDASFAPFEYMNKGEIVGFDVD 59 Query: 65 FIKAVMEEAGIDYTVTNTGWDTMLSSVKQGTEYQAGLSSVSITDERKETYDYSIPYFEST 124 +KAVM+EAG+ Y + N GWD + +++ QG +S ++I D+RK+TYD+SIPYFEST Sbjct: 60 LLKAVMKEAGLAYELKNIGWDPLFAAI-QGKTADMAVSGITINDDRKQTYDFSIPYFEST 118 Query: 125 NMIMVKEGSDIKNALDLKDKKVAVQAATTADDLMSGIMGVDNGNLKRFDSNAVALMELNS 184 +MI+VK+GS IK+A DL+ K V VQ TT + + I+G +N N+K+F++ VA+M+L + Sbjct: 119 HMILVKKGSSIKSAKDLQGKVVGVQNGTTGQEAVEKILGKENPNIKKFENTVVAIMDLLN 178 Query: 185 GGXXXXXXXXXXXNEYIKNNPNEKLTGIIDKENFGSEYYGILYPKGSEWKAKLDPAIKKI 244 GG NEY+KNNP+ K+ D E+F SE+YG ++PKGSE K K+D A+KK+ Sbjct: 179 GGVEAVVTDNAVANEYVKNNPDAKIVAFEDPEHFESEFYGFMFPKGSELKQKVDEALKKV 238 Query: 245 IENGKYAEIYKTWFGEEPDTQTL 267 I++G YAEIYK WFG+EP+ ++L Sbjct: 239 IKDGTYAEIYKKWFGKEPNVESL 261 >B4BPF0.1 B4BPF0_9BACI Extracellular solute-binding protein family 3 Length = 273 Score = 241 bits (616), Expect = 4e-62, Method: Compositional matrix adjust. Identities = 123/267 (46%), Positives = 174/267 (65%), Gaps = 6/267 (2%) Query: 5 KKWAMSAFVALLVLAIAXX----XXXXXXXXXXXXXETIKFASDASYAPMEYMDTDKIKG 60 KK + A VA L +A+A + I +DA++AP EYM+ K+ G Sbjct: 5 KKGFIVAVVAALFMALAACGKSTETTSSSGSEKETKQKITVGTDAAFAPFEYMEKGKVVG 64 Query: 61 FDIDFIKAVMEEAGIDYTVTNTGWDTMLSSVKQGTEYQAGLSSVSITDERKETYDYSIPY 120 FD D + AVM+EAG+DY + N GWD + +S+ Q E G+S ++ITD+RK++YD+S PY Sbjct: 65 FDADLLDAVMKEAGLDYELKNIGWDPLFASL-QSKEIDMGISGITITDKRKQSYDFSAPY 123 Query: 121 FESTNMIMVKEGSDIKNALDLKDKKVAVQAATTADDLMSGIMGVDNGNLKRFDSNAVALM 180 FE+T +I+VKEGS +KNALDLK K + VQ ATT + + G ++K+F+++ VA+M Sbjct: 124 FEATQVILVKEGSPVKNALDLKGKTIGVQNATTGQEAAEKLFG-KGEHIKKFETSVVAIM 182 Query: 181 ELNSGGXXXXXXXXXXXNEYIKNNPNEKLTGIIDKENFGSEYYGILYPKGSEWKAKLDPA 240 E+ +GG NEY+KNNP++KL I D +NF SEYYGI++PK S+ KAK+D A Sbjct: 183 EMLNGGVDAVITDNAVANEYVKNNPDKKLQVIEDPDNFTSEYYGIIFPKDSDLKAKVDEA 242 Query: 241 IKKIIENGKYAEIYKTWFGEEPDTQTL 267 +K IIENGKY E+YK WFG EP+ + L Sbjct: 243 LKNIIENGKYTEVYKKWFGIEPNIENL 269 >A4ITR8.1 A4ITR8_GEOTN Glutamine transport system substrate-binding protein Length = 273 Score = 241 bits (614), Expect = 7e-62, Method: Compositional matrix adjust. Identities = 123/267 (46%), Positives = 174/267 (65%), Gaps = 6/267 (2%) Query: 5 KKWAMSAFVALLVLAIAXX----XXXXXXXXXXXXXETIKFASDASYAPMEYMDTDKIKG 60 KK + A VA L +A+A + I +DA++AP EYM+ K+ G Sbjct: 5 KKGFIVAVVAALFMALAACGKSTETTSSSGSEKETKQKITVGTDAAFAPFEYMEKGKVVG 64 Query: 61 FDIDFIKAVMEEAGIDYTVTNTGWDTMLSSVKQGTEYQAGLSSVSITDERKETYDYSIPY 120 FD D + AVM+EAG+DY + N GWD + +S+ Q E G+S ++ITD+RK++YD+S PY Sbjct: 65 FDADLLDAVMKEAGLDYELKNIGWDPLFASL-QSKEIDMGISGITITDKRKQSYDFSAPY 123 Query: 121 FESTNMIMVKEGSDIKNALDLKDKKVAVQAATTADDLMSGIMGVDNGNLKRFDSNAVALM 180 FE+T +I+VKEGS +KNALDLK K + VQ ATT + + G ++K+F+++ VA+M Sbjct: 124 FEATQVILVKEGSLVKNALDLKGKTIGVQNATTGQEAAEKLFG-KGEHIKKFETSVVAIM 182 Query: 181 ELNSGGXXXXXXXXXXXNEYIKNNPNEKLTGIIDKENFGSEYYGILYPKGSEWKAKLDPA 240 E+ +GG NEY+KNNP++KL I D +NF SEYYGI++PK S+ KAK+D A Sbjct: 183 EMLNGGVDAVITDNAVANEYVKNNPDKKLQVIEDPDNFTSEYYGIIFPKDSDLKAKVDEA 242 Query: 241 IKKIIENGKYAEIYKTWFGEEPDTQTL 267 +K IIENGKY E+YK WFG EP+ + L Sbjct: 243 LKNIIENGKYTEVYKKWFGIEPNIENL 269 >C3IZE0.1 C3IZE0_9BACI Extracellular solute-binding protein family 3 Length = 273 Score = 238 bits (606), Expect = 5e-61, Method: Compositional matrix adjust. Identities = 114/226 (50%), Positives = 157/226 (69%), Gaps = 2/226 (0%) Query: 42 ASDASYAPMEYMDTDKIKGFDIDFIKAVMEEAGIDYTVTNTGWDTMLSSVKQGTEYQAGL 101 +DA++AP EYM KI GFD+D + AVM+ AG+DY + N GWD + +S+ Q E G+ Sbjct: 48 GTDAAFAPFEYMQKGKIVGFDVDLLDAVMKAAGLDYELKNIGWDPLFASL-QSKEVDMGI 106 Query: 102 SSVSITDERKETYDYSIPYFESTNMIMVKEGSDIKNALDLKDKKVAVQAATTADDLMSGI 161 S ++ITDERK++YD+S PYFE+T +I+VK+GS +KNALDLK K + VQ ATT + + Sbjct: 107 SGITITDERKQSYDFSDPYFEATQVILVKQGSPVKNALDLKGKTIGVQNATTGQEAAEKL 166 Query: 162 MGVDNGNLKRFDSNAVALMELNSGGXXXXXXXXXXXNEYIKNNPNEKLTGIIDKENFGSE 221 G ++K+F++ VA+MEL +GG NEY+KNNPN+KL I D +NF SE Sbjct: 167 FG-KGPHIKKFETTVVAIMELLNGGVDAVITDNAVANEYVKNNPNKKLQVIEDPKNFASE 225 Query: 222 YYGILYPKGSEWKAKLDPAIKKIIENGKYAEIYKTWFGEEPDTQTL 267 YYG+++PK SE KAK+D A+K +I +GKY EIYK WFG+EP L Sbjct: 226 YYGMIFPKNSELKAKVDEALKNVINSGKYTEIYKKWFGKEPKLDRL 271 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.312 0.130 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,425,810,010 Number of extensions: 54816745 Number of successful extensions: 130184 Number of sequences better than 1.0e-05: 3741 Number of HSP's gapped: 125882 Number of HSP's successfully gapped: 3813 Length of query: 271 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 138 Effective length of database: 1,807,701,208 Effective search space: 249462766704 Effective search space used: 249462766704 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 130 (54.7 bits)