BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0459.0023.p (247 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A4J1D4.1 A4J1D4_DESRM Integral membrane protein TerC 262 2e-68 C1TF60.1 C1TF60_9FIRM Membrane protein TerC, possibly involved i... 261 3e-68 A7Z141.1 A7Z141_BACA2 YceF 259 1e-67 O34447.1 YCEF_BACSU Uncharacterized membrane protein yceF 243 1e-62 C2W3M4.1 C2W3M4_BACCE Uncharacterized membrane protein yceF 236 1e-60 >A4J1D4.1 A4J1D4_DESRM Integral membrane protein TerC Length = 255 Score = 262 bits (670), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 139/258 (53%), Positives = 179/258 (69%), Gaps = 17/258 (6%) Query: 1 MDWFSDFFRSISDNYGHFFSWSDIVGTLSDPVSWGIIGSLVLLEGLLSADNALVLAVMVK 60 M+ + F + NYGHFFSW DI ++DPVSWGI+ LV+LEGLLS DNALVLAV+V Sbjct: 1 MEAVTTFLTGLVANYGHFFSWQDIHRVMTDPVSWGIVFWLVILEGLLSCDNALVLAVVVN 60 Query: 61 HLPKEQQKKALFYGILGAYLFRFLAIGLGTYLIGFWEVKVAGALYLFYIAYKGLFKXXXX 120 LPK QQKKAL YGI GAY FRF+AIGLGTYL+ V++ GA YL ++A G F Sbjct: 61 KLPKHQQKKALLYGIWGAYFFRFIAIGLGTYLVKIKWVQILGAGYLLWMA--GKFFLSKG 118 Query: 121 XXXTKNRGAS------------FWKTVLLVELMDIAFSIDSVIAAFGLSNEVWVLFLGGI 168 + G + FW TV+ VELMDI FS+DS++AAF +SNEVWVLF+GGI Sbjct: 119 EEADEGDGEAKVSGFWLKMVGPFWATVITVELMDITFSVDSILAAFAMSNEVWVLFMGGI 178 Query: 169 LGVLMMRGVAQLFLKLIARFPELEQTAFLLIAIIAGKMLAGAFGYEMPHVVFFTILIAVF 228 LG+LMMRGVA +F+KL+ ++PELE +A++LIA+I GK++ AFGY +PH +FF+I+ AV Sbjct: 179 LGILMMRGVATIFIKLLEKYPELEASAYVLIALIGGKLMGQAFGYHIPHGIFFSIMAAVL 238 Query: 229 VGTILYSSGKKKREANRK 246 +GTI+ RE K Sbjct: 239 LGTIVL---HHLREGGNK 253 >C1TF60.1 C1TF60_9FIRM Membrane protein TerC, possibly involved in tellurium resistance Length = 231 Score = 261 bits (668), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 133/226 (58%), Positives = 175/226 (77%), Gaps = 2/226 (0%) Query: 24 IVGTLSDPVSWGIIGSLVLLEGLLSADNALVLAVMVKHLPKEQQKKALFYGILGAYLFRF 83 I+ +S P SW +I SLV+LEGLLSADNALVLAV+VKHLP+EQ+KKALFYGI+GAY+FRF Sbjct: 4 IMDVVSSPASWAVIFSLVILEGLLSADNALVLAVVVKHLPEEQRKKALFYGIMGAYVFRF 63 Query: 84 LAIGLGTYLIGFWEVKVAGALYLFYIAYKGLFKXXXXXXXTKN-RGASFWKTVLLVELMD 142 +AIGLG Y++ F VK+ GA YL +IA K K + N + FW TV+ VELMD Sbjct: 64 IAIGLGVYIVKFTWVKILGASYLLWIALKFFIKKNNESEESVNVKATGFWGTVITVELMD 123 Query: 143 IAFSIDSVIAAFGLSNEVWVLFLGGILGVLMMRGVAQLFLKLIARFPELEQTAFLLIAII 202 I FS+DS++AAFG+S++VWVL+LGGILG+LMMRGVAQLFL L+ +FPELE TA++LIA+I Sbjct: 124 ITFSVDSILAAFGVSDKVWVLYLGGILGILMMRGVAQLFLLLLEKFPELEATAYILIALI 183 Query: 203 AGKMLAGAFGYEMPHVVFFTILIAVFVGTILYSSGKK-KREANRKV 247 K++ AFG +P VFF ++ +VF+GT++YS +K K +A K+ Sbjct: 184 GAKLMGSAFGLHLPEFVFFPVMASVFIGTLVYSHFRKDKSKAEEKI 229 >A7Z141.1 A7Z141_BACA2 YceF Length = 257 Score = 259 bits (662), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 138/249 (55%), Positives = 173/249 (69%), Gaps = 8/249 (3%) Query: 6 DFFRSISDNYGHFFSWSDIVGTLSDPVSWGIIGSLVLLEGLLSADNALVLAVMVKHLPKE 65 DF I Y FF W L+DPVSWG+IGSLVLLEGLLSADNALVLAVMV+HLP++ Sbjct: 2 DFLHHIMSTYASFFDWKMWGEVLTDPVSWGLIGSLVLLEGLLSADNALVLAVMVRHLPEK 61 Query: 66 QQKKALFYGILGAYLFRFLAIGLGTYLIGFWEVKVAGALYLFYIAYKGLFKXXXXXXXTK 125 Q+KKAL YG+ GAY+FRF+ IG+G LI FW +KV GALYL ++ K + Sbjct: 62 QRKKALTYGLFGAYIFRFIFIGIGMLLIKFWWIKVLGALYLAWLVIKHFWIGEGDDDTEG 121 Query: 126 NRGAS--------FWKTVLLVELMDIAFSIDSVIAAFGLSNEVWVLFLGGILGVLMMRGV 177 + S FW TV+ VELMD+AFS+DS++AAF +S +VWVL +GG+LG+LMMR V Sbjct: 122 MKKNSWMVRTFGIFWATVVSVELMDLAFSVDSILAAFAVSEKVWVLLIGGMLGILMMRTV 181 Query: 178 AQLFLKLIARFPELEQTAFLLIAIIAGKMLAGAFGYEMPHVVFFTILIAVFVGTILYSSG 237 A++FL LI + PELE TAF+LI IIA KM A AF YEMPHVVFF I+IA FV T + Sbjct: 182 AKVFLVLIDKIPELENTAFVLIGIIAVKMAASAFDYEMPHVVFFGIIIAAFVVTFIIHYF 241 Query: 238 KKKREANRK 246 K+++ + Sbjct: 242 NKQKQVQEQ 250 >O34447.1 YCEF_BACSU Uncharacterized membrane protein yceF Length = 257 Score = 243 bits (619), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 138/249 (55%), Positives = 172/249 (69%), Gaps = 8/249 (3%) Query: 6 DFFRSISDNYGHFFSWSDIVGTLSDPVSWGIIGSLVLLEGLLSADNALVLAVMVKHLPKE 65 DF I Y FF W L+DPVSWG+IGSLV+LEGLLSADNALVLAVMVKHLP++ Sbjct: 2 DFLHHILSTYASFFDWKMWGEVLTDPVSWGLIGSLVVLEGLLSADNALVLAVMVKHLPEK 61 Query: 66 QQKKALFYGILGAYLFRFLAIGLGTYLIGFWEVKVAGALYLFYIAYKGLF---KXXXXXX 122 Q+KKAL YG+ GAY+FRF+ IGLG LI FW +KV GALYL ++ K + K Sbjct: 62 QRKKALTYGLFGAYIFRFIFIGLGMLLIKFWWIKVLGALYLAWLVIKHFWIGEKEEEADG 121 Query: 123 XTKNRGAS-----FWKTVLLVELMDIAFSIDSVIAAFGLSNEVWVLFLGGILGVLMMRGV 177 KN FW TV+ VELMD+AFS+DS++AAF +S +VWVL +GG+LG+LMMR V Sbjct: 122 MKKNSWMVRTFGIFWATVISVELMDLAFSVDSILAAFAVSEKVWVLLIGGMLGILMMRTV 181 Query: 178 AQLFLKLIARFPELEQTAFLLIAIIAGKMLAGAFGYEMPHVVFFTILIAVFVGTILYSSG 237 A++FL LI + PELE TAF+LI IIA KM AF YEMPH VFF I+IA F T++ Sbjct: 182 AKVFLVLIDKIPELENTAFVLIGIIALKMAGSAFHYEMPHSVFFIIIIAAFAVTLIIHYI 241 Query: 238 KKKREANRK 246 K+++ + Sbjct: 242 NKQKQVREQ 250 >C2W3M4.1 C2W3M4_BACCE Uncharacterized membrane protein yceF Length = 262 Score = 236 bits (603), Expect = 1e-60, Method: Compositional matrix adjust. Identities = 138/244 (56%), Positives = 175/244 (71%), Gaps = 14/244 (5%) Query: 13 DNYGHFFSWSDIVGTLSDPVSWGIIGSLVLLEGLLSADNALVLAVMVKHLPKEQQKKALF 72 D Y FF W + L DPVSWG+IG+LV+LEGLLSADNALVLAVMVKHLP+E++KKALF Sbjct: 9 DTYAQFFDWDMWIKVLQDPVSWGLIGTLVVLEGLLSADNALVLAVMVKHLPEEKRKKALF 68 Query: 73 YGILGAYLFRFLAIGLGTYLIGFWEVKVAGALYLFYIAYKGLF-KXXXXXXXTKNRGAS- 130 YG++GAY+FRF+AIG+G +LI W VK+ GALYL +++ K K + G + Sbjct: 69 YGLIGAYVFRFIAIGIGMFLIKLWWVKLLGALYLAWLSIKYFMDKRKGNDEEEEAHGMNE 128 Query: 131 ----------FWKTVLLVELMDIAFSIDSVIAAFGLSNEVWVLFLGGILGVLMMRGVAQL 180 FW TV++VELMDIAFS+DSV+AAFG+SNEVW+L LGG+LG+LMMRG+A + Sbjct: 129 NSILFRMFGVFWGTVVMVELMDIAFSVDSVLAAFGVSNEVWILLLGGMLGILMMRGIAGV 188 Query: 181 FLKLIARFPELEQTAFLLIAIIAGKMLAGAFGYEMPHVVFFTILIAVFVGTIL--YSSGK 238 FLKL+ R PELE TA++LI IIAGKML + EMPH +FF IL+ F T + Y Sbjct: 189 FLKLLERIPELESTAYILILIIAGKMLLSVWHIEMPHWLFFIILVIAFGATFILHYMRKG 248 Query: 239 KKRE 242 + RE Sbjct: 249 QARE 252 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.330 0.145 0.441 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,383,862,573 Number of extensions: 54120498 Number of successful extensions: 214301 Number of sequences better than 1.0e-05: 1562 Number of HSP's gapped: 212020 Number of HSP's successfully gapped: 1566 Length of query: 247 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 115 Effective length of database: 1,817,122,223 Effective search space: 208969055645 Effective search space used: 208969055645 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 129 (54.3 bits)