BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0459.0019.p (277 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B7IMY1.1 B7IMY1_BACC2 Putative uncharacterized protein 181 7e-44 C3DS86.1 C3DS86_BACTS Hydrolase (HAD superfamily) 181 7e-44 Q3EZH6.1 Q3EZH6_BACTI Hydrolase (HAD superfamily) 181 8e-44 B7HBK4.1 B7HBK4_BACC4 Putative uncharacterized protein 181 9e-44 C0Z9Y8.1 C0Z9Y8_BREBN Putative uncharacterized protein 179 2e-43 >B7IMY1.1 B7IMY1_BACC2 Putative uncharacterized protein Length = 271 Score = 181 bits (458), Expect = 7e-44, Method: Compositional matrix adjust. Identities = 100/276 (36%), Positives = 154/276 (55%), Gaps = 15/276 (5%) Query: 1 MIYASDLDRTLIYSPSAIGVPGDSPGLVPAEVVDGRTVSYISQHALDLLKELAAKIVFMP 60 MI+ASDLD+TLIYS + P + + E +DG+ +S+IS + LLK+L F+P Sbjct: 1 MIFASDLDQTLIYSRKSFRAPIEDESIQLIETLDGKEISFISHKTISLLKKLQLHSHFIP 60 Query: 61 VTTRTMAQYRRINLFQETVIPHYAITSNGGNILVNGVVDQEWRAAVGRLVERGSAPAEEA 120 VTTRT+ Q++RI LFQ +IP YAITSNGGNIL NG D W V ER S E Sbjct: 61 VTTRTIEQFQRITLFQNEIIPEYAITSNGGNILHNGKQDGTWNRTVK---ERLSVECIER 117 Query: 121 GRLVR---SVVRTGWIISERYCDELFYTFVVHRDLLPMDEITRLAEHLNGWGWKLSLQGR 177 +++ + W+IS++ D+LF+ ++ R+ +P DE++ L+ GW SLQGR Sbjct: 118 NDILKEFGQIAHKDWVISQKTADDLFHYCIIKRENIPYDELSSFTSWLDKQGWNHSLQGR 177 Query: 178 KLYVVPEAVNKSDAILHVRRTADSGPMVXXXXXXXXXXXXXXXXXXIAPCHGEIFAEQ-- 235 KLY VP+ +NK DA+ +++ ++ AP HGE+ A Sbjct: 178 KLYFVPKPINKWDAVQYIKEILQIDTIITAGDSLLDLCMLEKANHAFAPLHGELGAMHDN 237 Query: 236 -QAGLVKLAYPFTKQAGVFAGDEIM-EYVQRVYNNL 269 Q+ ++K T G++A +EI+ + +Q ++++L Sbjct: 238 LQSYILK-----TDAIGIYAAEEIVNKSLQIIHSSL 268 >C3DS86.1 C3DS86_BACTS Hydrolase (HAD superfamily) Length = 271 Score = 181 bits (458), Expect = 7e-44, Method: Compositional matrix adjust. Identities = 100/276 (36%), Positives = 154/276 (55%), Gaps = 15/276 (5%) Query: 1 MIYASDLDRTLIYSPSAIGVPGDSPGLVPAEVVDGRTVSYISQHALDLLKELAAKIVFMP 60 MI+ASDLD+TLIYS + P + + E +DG+ +S+IS + LLK+L F+P Sbjct: 1 MIFASDLDQTLIYSRKSFRAPIEDESIQLIETLDGKEISFISHKTISLLKKLQLHSHFIP 60 Query: 61 VTTRTMAQYRRINLFQETVIPHYAITSNGGNILVNGVVDQEWRAAVGRLVERGSAPAEEA 120 VTTRT+ Q++RI LFQ +IP YAITSNGGNIL NG D W V ER S E Sbjct: 61 VTTRTIEQFQRITLFQNEIIPEYAITSNGGNILHNGKQDGTWNRTVK---ERLSVECIER 117 Query: 121 GRLVR---SVVRTGWIISERYCDELFYTFVVHRDLLPMDEITRLAEHLNGWGWKLSLQGR 177 +++ + W+IS++ D+LF+ ++ R+ +P DE++ L+ GW SLQGR Sbjct: 118 NDILKEFGQIAHKDWVISQKTADDLFHYCIIKRENIPYDELSSFTSWLDKQGWNHSLQGR 177 Query: 178 KLYVVPEAVNKSDAILHVRRTADSGPMVXXXXXXXXXXXXXXXXXXIAPCHGEIFAEQ-- 235 KLY VP+ +NK DA+ +++ ++ AP HGE+ A Sbjct: 178 KLYFVPKPINKWDAVQYIKEILQIDTIITAGDSLLDLCMLEKANHAFAPLHGELGAMHDN 237 Query: 236 -QAGLVKLAYPFTKQAGVFAGDEIM-EYVQRVYNNL 269 Q+ ++K T G++A +EI+ + +Q ++++L Sbjct: 238 LQSYILK-----TDAIGIYAAEEIVNKSLQIIHSSL 268 >Q3EZH6.1 Q3EZH6_BACTI Hydrolase (HAD superfamily) Length = 271 Score = 181 bits (458), Expect = 8e-44, Method: Compositional matrix adjust. Identities = 100/276 (36%), Positives = 154/276 (55%), Gaps = 15/276 (5%) Query: 1 MIYASDLDRTLIYSPSAIGVPGDSPGLVPAEVVDGRTVSYISQHALDLLKELAAKIVFMP 60 MI+ASDLD+TLIYS + P + + E +DG+ +S+IS + LLK+L F+P Sbjct: 1 MIFASDLDQTLIYSRKSFRAPIEDESIQLIETLDGKEISFISHKTISLLKKLQLHSHFIP 60 Query: 61 VTTRTMAQYRRINLFQETVIPHYAITSNGGNILVNGVVDQEWRAAVGRLVERGSAPAEEA 120 VTTRT+ Q++RI LFQ +IP YAITSNGGNIL NG D W V ER S E Sbjct: 61 VTTRTIEQFQRITLFQNEIIPEYAITSNGGNILHNGKQDGTWNRTVK---ERLSVECIER 117 Query: 121 GRLVR---SVVRTGWIISERYCDELFYTFVVHRDLLPMDEITRLAEHLNGWGWKLSLQGR 177 +++ + W+IS++ D+LF+ ++ R+ +P DE++ L+ GW SLQGR Sbjct: 118 NDILKEFGQIAHKDWVISQKTADDLFHYCIIKRENIPYDELSSFTSWLDKQGWNHSLQGR 177 Query: 178 KLYVVPEAVNKSDAILHVRRTADSGPMVXXXXXXXXXXXXXXXXXXIAPCHGEIFAEQ-- 235 KLY VP+ +NK DA+ +++ ++ AP HGE+ A Sbjct: 178 KLYFVPKPINKWDAVQYIKEILQIDTIITAGDSLRDLCMLEKANHAFAPLHGELGAMHDN 237 Query: 236 -QAGLVKLAYPFTKQAGVFAGDEIM-EYVQRVYNNL 269 Q+ ++K T G++A +EI+ + +Q ++++L Sbjct: 238 LQSYILK-----TDAIGIYAAEEIVNKSLQIIHSSL 268 >B7HBK4.1 B7HBK4_BACC4 Putative uncharacterized protein Length = 271 Score = 181 bits (458), Expect = 9e-44, Method: Compositional matrix adjust. Identities = 100/276 (36%), Positives = 155/276 (56%), Gaps = 15/276 (5%) Query: 1 MIYASDLDRTLIYSPSAIGVPGDSPGLVPAEVVDGRTVSYISQHALDLLKELAAKIVFMP 60 MI+ASDLD+TLIYS + P + + E +DG+ +S+IS + LLK+L F+P Sbjct: 1 MIFASDLDQTLIYSRKSFRAPIEDESIQLIETLDGKEISFISHKTISLLKKLQLHSHFIP 60 Query: 61 VTTRTMAQYRRINLFQETVIPHYAITSNGGNILVNGVVDQEWRAAVGRLVERGSAPAEEA 120 VTTRT+ Q++RI+LFQ+ +IP YAITSNGGNIL NG D W V ER S E Sbjct: 61 VTTRTIEQFQRISLFQDEIIPEYAITSNGGNILHNGKQDGTWNRIVK---ERLSFECIEG 117 Query: 121 GRLVR---SVVRTGWIISERYCDELFYTFVVHRDLLPMDEITRLAEHLNGWGWKLSLQGR 177 +++ + W+IS++ D+LF+ ++ R+ +P DE++ L+ GW SLQGR Sbjct: 118 NDILKEFGQIAHKDWVISQKTADDLFHYCIIKRENIPYDELSSFTSWLDKQGWNHSLQGR 177 Query: 178 KLYVVPEAVNKSDAILHVRRTADSGPMVXXXXXXXXXXXXXXXXXXIAPCHGEIFAEQ-- 235 KLY VP+ +NK DA+ +++ ++ AP HGE+ A Sbjct: 178 KLYFVPKPINKWDAVQYIKEILQIDTIITAGDSLLDLCMLEKANHAFAPLHGELGAMHDS 237 Query: 236 -QAGLVKLAYPFTKQAGVFAGDEIM-EYVQRVYNNL 269 Q ++K T G++A +EI+ + +Q ++++L Sbjct: 238 LQPHILK-----TDAIGIYAAEEIVNKSLQIIHSSL 268 >C0Z9Y8.1 C0Z9Y8_BREBN Putative uncharacterized protein Length = 271 Score = 179 bits (455), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 95/269 (35%), Positives = 152/269 (56%), Gaps = 2/269 (0%) Query: 1 MIYASDLDRTLIYSPSAIGVPGDSPGLVPAEVVDGRTVSYISQHALDLLKELAAKIVFMP 60 MI+ASDLD+TL+Y + S +V EV++G +S++S A++LL E+ K +F+P Sbjct: 1 MIFASDLDQTLLYGKRFLTDEMQSFQVV--EVLEGEPISHMSDRAIELLHEVHQKSLFIP 58 Query: 61 VTTRTMAQYRRINLFQETVIPHYAITSNGGNILVNGVVDQEWRAAVGRLVERGSAPAEEA 120 VTTRT+ QY+RI +FQ ++P YAITSNGGN++ +G +D++W V + AP + Sbjct: 59 VTTRTIEQYQRILIFQNELLPRYAITSNGGNVIRDGRIDEQWSRQVHSRMVNECAPFPDM 118 Query: 121 GRLVRSVVRTGWIISERYCDELFYTFVVHRDLLPMDEITRLAEHLNGWGWKLSLQGRKLY 180 + W+I ER ++LF+ ++ R+ +P+DE+ + W +S+QGRKLY Sbjct: 119 LAGFAQISNDSWVIRERMAEDLFFYSIIDREKMPVDELRDFERWVAQQNWSMSIQGRKLY 178 Query: 181 VVPEAVNKSDAILHVRRTADSGPMVXXXXXXXXXXXXXXXXXXIAPCHGEIFAEQQAGLV 240 +VP+AVNK DA L+V ++ I P HGEI+ + G Sbjct: 179 LVPQAVNKRDATLYVCELEQDEQLIAAGDSLLDLPLLEAAQLAIVPPHGEIYQIRNHGGA 238 Query: 241 KLAYPFTKQAGVFAGDEIMEYVQRVYNNL 269 + FT+ +G+ A +EI++ V Y+ L Sbjct: 239 VGNFHFTQASGISAAEEILQTVLSWYHQL 267 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,514,626,661 Number of extensions: 56613087 Number of successful extensions: 139094 Number of sequences better than 1.0e-05: 48 Number of HSP's gapped: 139006 Number of HSP's successfully gapped: 48 Length of query: 277 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 143 Effective length of database: 1,798,280,193 Effective search space: 257154067599 Effective search space used: 257154067599 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)