BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0459.0015.p (268 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K6V6.1 B3K6V6_9BACI Formate/nitrite transporter 415 e-114 Q65DB6.1 Q65DB6_BACLD YwcJ 349 2e-94 A8HU49.1 A8HU49_BACSU Putative formate/nitrite transporter 317 5e-85 P39608.1 YWCJ_BACSU Uncharacterized transporter ywcJ 317 5e-85 A8HU81.1 A8HU81_BACSU Putative formate/nitrite transporter 313 7e-84 >B3K6V6.1 B3K6V6_9BACI Formate/nitrite transporter Length = 265 Score = 415 bits (1066), Expect = e-114, Method: Compositional matrix adjust. Identities = 198/263 (75%), Positives = 223/263 (84%) Query: 1 MENEALLQVEKLALKKQKIYQQSIFRYIARAMLASMFIGFGVIVAFKTGNFFYMEHSPMT 60 ME E+LL+VE+LALKK KI++QS RYIAR+MLASMFIGFGVIVAFKTGNFFY+EHSP+T Sbjct: 1 METESLLKVEQLALKKHKIFKQSQLRYIARSMLASMFIGFGVIVAFKTGNFFYLEHSPLT 60 Query: 61 YPMAAITFGAAIILISYGGGDLFTGDTFYYTYAALRRKLQWTEVVRMWVISYIGNILGAT 120 YPMAA+TFGAAIILI+YGGGDLFTG+TFYYTYAALR+KL+W EVV++W+ SY GNILGA Sbjct: 61 YPMAALTFGAAIILIAYGGGDLFTGNTFYYTYAALRKKLKWLEVVKLWIFSYAGNILGAC 120 Query: 121 AFALLIFLTGLFYDSSVNGFLLNVVAHKMEAPAMQLFFRAILCNWLVCLAFFVPMSMKSD 180 FALLIFLTGLF DSSVNGFLLNVV HKM P +QLFFRAILCNWLVCLAFF+PMS+K D Sbjct: 121 TFALLIFLTGLFTDSSVNGFLLNVVEHKMTTPTVQLFFRAILCNWLVCLAFFIPMSLKGD 180 Query: 181 GAKMFAMVLFVFCFFISGYEHSIANMCTFAIALVLDHPGTISWGGVVHNLVPVTXXXXXX 240 GAKMFAM+LFVFCFFISGYEHS+ANMCTFAIALVL+HPGTIS+ GV+HNL+PVT Sbjct: 181 GAKMFAMMLFVFCFFISGYEHSVANMCTFAIALVLNHPGTISFDGVLHNLIPVTFGNLIG 240 Query: 241 XXXXXXXXXXXXXKPFLDEPHDE 263 KPFLD Sbjct: 241 GVLLMGFMYYFVNKPFLDAKESS 263 >Q65DB6.1 Q65DB6_BACLD YwcJ Length = 262 Score = 349 bits (895), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 165/234 (70%), Positives = 196/234 (83%) Query: 1 MENEALLQVEKLALKKQKIYQQSIFRYIARAMLASMFIGFGVIVAFKTGNFFYMEHSPMT 60 ME+++L+ VE+LALKKQ I+ QS RY+ RAMLASMFIGFGVIVAFKTGN FY+EHSP T Sbjct: 1 MESKSLVLVEELALKKQSIFVQSPLRYLLRAMLASMFIGFGVIVAFKTGNLFYVEHSPFT 60 Query: 61 YPMAAITFGAAIILISYGGGDLFTGDTFYYTYAALRRKLQWTEVVRMWVISYIGNILGAT 120 YP+AAITFG AIILI YGGGDLFTG+TFYYT AALR+K++W V+++W+ SY GN++GA Sbjct: 61 YPIAAITFGGAIILIVYGGGDLFTGNTFYYTLAALRKKMRWPTVIKLWMFSYAGNLMGAV 120 Query: 121 AFALLIFLTGLFYDSSVNGFLLNVVAHKMEAPAMQLFFRAILCNWLVCLAFFVPMSMKSD 180 FA LIF TGLF D S GF+L+V HKM P +LFF+AILCNWLVCLAFFVPM+ K D Sbjct: 121 FFAGLIFATGLFKDPSSTGFILSVAEHKMNTPVTELFFKAILCNWLVCLAFFVPMAQKGD 180 Query: 181 GAKMFAMVLFVFCFFISGYEHSIANMCTFAIALVLDHPGTISWGGVVHNLVPVT 234 GAKMF+M+LFVF FFISGYEHSIANM TFA++LV +HP TIS G +HNL+PVT Sbjct: 181 GAKMFSMMLFVFTFFISGYEHSIANMATFAVSLVTEHPETISIAGAIHNLIPVT 234 >A8HU49.1 A8HU49_BACSU Putative formate/nitrite transporter Length = 256 Score = 317 bits (813), Expect = 5e-85, Method: Compositional matrix adjust. Identities = 143/234 (61%), Positives = 191/234 (81%) Query: 1 MENEALLQVEKLALKKQKIYQQSIFRYIARAMLASMFIGFGVIVAFKTGNFFYMEHSPMT 60 ME +AL +VE+ ALKKQ I+ S RY+ R++LAS+FIGFG+ A KTG++F+M SP Sbjct: 1 METQALQKVEQYALKKQNIFASSKIRYVLRSILASIFIGFGITAASKTGSYFFMADSPFA 60 Query: 61 YPMAAITFGAAIILISYGGGDLFTGDTFYYTYAALRRKLQWTEVVRMWVISYIGNILGAT 120 +P AA+TFGAAI++I+YGGGDLFTG+TFY+TY ALR+K+ W + + +W+ SY GN++GA Sbjct: 61 FPAAAVTFGAAILMIAYGGGDLFTGNTFYFTYTALRKKISWRDTLYLWMSSYAGNLIGAI 120 Query: 121 AFALLIFLTGLFYDSSVNGFLLNVVAHKMEAPAMQLFFRAILCNWLVCLAFFVPMSMKSD 180 FA+LI TGLF + SV+ FL+++ HKME PA +LFFR +LCNWLVCLAFF+PMS+K + Sbjct: 121 LFAILISATGLFEEPSVHSFLIHLAEHKMEPPASELFFRGMLCNWLVCLAFFIPMSLKGE 180 Query: 181 GAKMFAMVLFVFCFFISGYEHSIANMCTFAIALVLDHPGTISWGGVVHNLVPVT 234 GAK+F M+LFVFCFFISG+EHSIANMCTFAI+L+++HP T++ G V NL+PVT Sbjct: 181 GAKLFTMMLFVFCFFISGFEHSIANMCTFAISLLIEHPDTVTLMGAVRNLIPVT 234 >P39608.1 YWCJ_BACSU Uncharacterized transporter ywcJ Length = 256 Score = 317 bits (813), Expect = 5e-85, Method: Compositional matrix adjust. Identities = 143/234 (61%), Positives = 191/234 (81%) Query: 1 MENEALLQVEKLALKKQKIYQQSIFRYIARAMLASMFIGFGVIVAFKTGNFFYMEHSPMT 60 ME +AL +VE+ ALKKQ I+ S RY+ R++LAS+FIGFG+ A KTG++F+M SP Sbjct: 1 METQALQKVEQYALKKQNIFASSKIRYVLRSILASIFIGFGITAASKTGSYFFMADSPFA 60 Query: 61 YPMAAITFGAAIILISYGGGDLFTGDTFYYTYAALRRKLQWTEVVRMWVISYIGNILGAT 120 +P AA+TFGAAI++I+YGGGDLFTG+TFY+TY ALR+K+ W + + +W+ SY GN++GA Sbjct: 61 FPAAAVTFGAAILMIAYGGGDLFTGNTFYFTYTALRKKISWRDTLYLWMSSYAGNLIGAI 120 Query: 121 AFALLIFLTGLFYDSSVNGFLLNVVAHKMEAPAMQLFFRAILCNWLVCLAFFVPMSMKSD 180 FA+LI TGLF + SV+ FL+++ HKME PA +LFFR +LCNWLVCLAFF+PMS+K + Sbjct: 121 LFAILISATGLFEEPSVHSFLIHLAEHKMEPPASELFFRGMLCNWLVCLAFFIPMSLKGE 180 Query: 181 GAKMFAMVLFVFCFFISGYEHSIANMCTFAIALVLDHPGTISWGGVVHNLVPVT 234 GAK+F M+LFVFCFFISG+EHSIANMCTFAI+L+++HP T++ G V NL+PVT Sbjct: 181 GAKLFTMMLFVFCFFISGFEHSIANMCTFAISLLIEHPDTVTLMGAVRNLIPVT 234 >A8HU81.1 A8HU81_BACSU Putative formate/nitrite transporter Length = 256 Score = 313 bits (803), Expect = 7e-84, Method: Compositional matrix adjust. Identities = 143/234 (61%), Positives = 190/234 (81%) Query: 1 MENEALLQVEKLALKKQKIYQQSIFRYIARAMLASMFIGFGVIVAFKTGNFFYMEHSPMT 60 ME +AL +VE+ AL K I+ S RY+ R++LASMFIGFG+ A KTG++F M SP Sbjct: 1 METQALQKVEQYALNKLNIFVSSKIRYVLRSILASMFIGFGITAASKTGSYFSMADSPFA 60 Query: 61 YPMAAITFGAAIILISYGGGDLFTGDTFYYTYAALRRKLQWTEVVRMWVISYIGNILGAT 120 +P AA+TFGAAI+LI+YGGGDLFTG+TFY+TY ALR+K++W + + +W+ SY GN++GA Sbjct: 61 FPAAAVTFGAAILLIAYGGGDLFTGNTFYFTYTALRKKIRWRDTLYLWMSSYAGNLIGAI 120 Query: 121 AFALLIFLTGLFYDSSVNGFLLNVVAHKMEAPAMQLFFRAILCNWLVCLAFFVPMSMKSD 180 FA+LI TGLF ++SV+ FL+++ HKME PA +LFFR +LCNWLVCLAFF+PMS+K + Sbjct: 121 LFAILISATGLFEEASVHSFLIHLAEHKMEPPASELFFRGMLCNWLVCLAFFIPMSLKGE 180 Query: 181 GAKMFAMVLFVFCFFISGYEHSIANMCTFAIALVLDHPGTISWGGVVHNLVPVT 234 GAK+F M+LFVFCFFISG+EHSIANMCTFAI+L+++HP T++ G V NL+PVT Sbjct: 181 GAKLFTMMLFVFCFFISGFEHSIANMCTFAISLLIEHPDTVTVMGAVRNLIPVT 234 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.331 0.142 0.444 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,453,859,641 Number of extensions: 55468818 Number of successful extensions: 173575 Number of sequences better than 1.0e-05: 1174 Number of HSP's gapped: 171945 Number of HSP's successfully gapped: 1174 Length of query: 268 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 135 Effective length of database: 1,807,701,208 Effective search space: 244039663080 Effective search space used: 244039663080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 130 (54.7 bits)