BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0459.0014.p (325 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1BBV5.1 B1BBV5_CLOBO Transposase 439 e-121 C4IXR1.1 C4IXR1_CLOBO Putative transposase OrfB 431 e-119 C3KS58.1 C3KS58_CLOB6 Transposase 431 e-119 B1INY3.1 B1INY3_CLOBK Transposase 431 e-119 C3HBT9.1 C3HBT9_BACTU Transposase, IS605 OrfB 424 e-117 >B1BBV5.1 B1BBV5_CLOBO Transposase Length = 362 Score = 439 bits (1129), Expect = e-121, Method: Compositional matrix adjust. Identities = 214/330 (64%), Positives = 256/330 (77%), Gaps = 5/330 (1%) Query: 1 MLADKIEHYKQTETM-----LNNTPAQYKKDFDWLKEVDSLALANAQLNLDKAYKNFFRD 55 ML+D+I+ Y++ + + TPAQYKK+F WLKEVDSLALANAQ+NLDKAYKNFFRD Sbjct: 33 MLSDRIKSYEENKDLDIKKVKYPTPAQYKKEFTWLKEVDSLALANAQMNLDKAYKNFFRD 92 Query: 56 XXXXXXXXXXXXTNNHSFTTNNQNGTVAIENGXXXXXXXXXXXXXXXHRQLVGLIKSCTI 115 +N S+TTNNQ GTV IENG HRQ GLIKSCTI Sbjct: 93 KSVGFPKFKSKKSNRFSYTTNNQKGTVYIENGYIKIPKLKSMIKVKLHRQFNGLIKSCTI 152 Query: 116 SKTPTGKYYAAVLVETGVISLPKLESKIGVDLGLKDFAITSHGEIIPNPKYLRTSEKRLV 175 SKTP+ KYY ++L++T I LPK+E+KIGVD+GLK+FA+ S+G+ NPK+LR + KRL Sbjct: 153 SKTPSNKYYISILIDTENIKLPKVENKIGVDVGLKEFAVCSNGDRYENPKWLRKTTKRLA 212 Query: 176 KLQKDLARKKKGSHNRYKARLKVAKLHEKIANQRKDFLHKLSTQMINDNQVIVMEDLRVK 235 KLQKDL+RKKKGS+NRYKARLKVAKLHEKIANQRKDFLHKLS ++I +NQ IV+EDL+VK Sbjct: 213 KLQKDLSRKKKGSNNRYKARLKVAKLHEKIANQRKDFLHKLSIKLIRENQSIVIEDLKVK 272 Query: 236 NMMQNHKLAKAISEVSWSMFREILTYKSKWYGRELIIAPKNFASSQLCSCCGYKNAEVKN 295 NM+QNH LAKAISEVSW FR +L YK++WYGR +IIAP N+ASSQLCS CGYKN EVKN Sbjct: 273 NMLQNHNLAKAISEVSWYEFRTMLEYKAEWYGRNIIIAPSNYASSQLCSNCGYKNKEVKN 332 Query: 296 LGVRQWTCPECTTLHDRDVNAAKNLLKLAM 325 LG+R+W CP+C T HDRDVNA NLLKLA+ Sbjct: 333 LGLREWICPQCGTHHDRDVNAGINLLKLAI 362 >C4IXR1.1 C4IXR1_CLOBO Putative transposase OrfB Length = 365 Score = 431 bits (1109), Expect = e-119, Method: Compositional matrix adjust. Identities = 211/330 (63%), Positives = 254/330 (76%), Gaps = 5/330 (1%) Query: 1 MLADKIEHYKQTETM-----LNNTPAQYKKDFDWLKEVDSLALANAQLNLDKAYKNFFRD 55 ML+D+I+ Y++ + + TPAQYKK+F WLKEVDSLALANAQ+NLDKAYKNFFRD Sbjct: 36 MLSDRIKLYEENKDLDIKKVKYPTPAQYKKEFTWLKEVDSLALANAQMNLDKAYKNFFRD 95 Query: 56 XXXXXXXXXXXXTNNHSFTTNNQNGTVAIENGXXXXXXXXXXXXXXXHRQLVGLIKSCTI 115 TN +S+TTNNQ GTV IE+G HR+ GLIKSCTI Sbjct: 96 KSMGFPKFKSKKTNYYSYTTNNQKGTVYIEDGYIKLPKLKTMIKIKQHRKFNGLIKSCTI 155 Query: 116 SKTPTGKYYAAVLVETGVISLPKLESKIGVDLGLKDFAITSHGEIIPNPKYLRTSEKRLV 175 SKTP+ KYY ++LV+T LPK++ K+G+D+GLK+FAITS GE NPK+LR SEKRL Sbjct: 156 SKTPSNKYYISILVDTENKQLPKVDKKVGIDVGLKEFAITSDGEFFSNPKWLRKSEKRLR 215 Query: 176 KLQKDLARKKKGSHNRYKARLKVAKLHEKIANQRKDFLHKLSTQMINDNQVIVMEDLRVK 235 KLQKDL+RK+KG +NR KARLKVAKLHEKI NQRK+FLHKLS ++I +NQ IV+EDL+VK Sbjct: 216 KLQKDLSRKQKGGNNRCKARLKVAKLHEKITNQRKNFLHKLSIKLIRENQSIVIEDLKVK 275 Query: 236 NMMQNHKLAKAISEVSWSMFREILTYKSKWYGRELIIAPKNFASSQLCSCCGYKNAEVKN 295 NM+QNHKLAKAISEVSW FR IL YK+ WYGRELIIAP N+ASSQ+CS CGYKN EVKN Sbjct: 276 NMLQNHKLAKAISEVSWYEFRTILEYKADWYGRELIIAPSNYASSQICSNCGYKNKEVKN 335 Query: 296 LGVRQWTCPECTTLHDRDVNAAKNLLKLAM 325 L +R+W CP+C HDRD+NA+KNLLKLA+ Sbjct: 336 LELREWVCPKCGIHHDRDINASKNLLKLAI 365 >C3KS58.1 C3KS58_CLOB6 Transposase Length = 362 Score = 431 bits (1108), Expect = e-119, Method: Compositional matrix adjust. Identities = 209/330 (63%), Positives = 251/330 (76%), Gaps = 5/330 (1%) Query: 1 MLADKIEHYKQTETM-----LNNTPAQYKKDFDWLKEVDSLALANAQLNLDKAYKNFFRD 55 MLAD+I+ Y++ + + TPAQYK +F WLKEVDSLALAN QLNLDKAYKNFFRD Sbjct: 33 MLADRIKSYEENKNLDIKQIKYPTPAQYKTEFPWLKEVDSLALANEQLNLDKAYKNFFRD 92 Query: 56 XXXXXXXXXXXXTNNHSFTTNNQNGTVAIENGXXXXXXXXXXXXXXXHRQLVGLIKSCTI 115 N +S+TTNNQ GT+ IE+ HRQ GLIKSCTI Sbjct: 93 KSVGFPKFKSKKINYYSYTTNNQKGTIYIESNKIKIPKLKTMIKIKQHRQFDGLIKSCTI 152 Query: 116 SKTPTGKYYAAVLVETGVISLPKLESKIGVDLGLKDFAITSHGEIIPNPKYLRTSEKRLV 175 SK P+G+YY ++LV+T I LP + KIGVD+GLK+FAI S G PNPK+LR SEKRL Sbjct: 153 SKNPSGRYYISILVDTENIILPNTDKKIGVDMGLKEFAICSDGYREPNPKHLRKSEKRLA 212 Query: 176 KLQKDLARKKKGSHNRYKARLKVAKLHEKIANQRKDFLHKLSTQMINDNQVIVMEDLRVK 235 KLQKDL+RK+KGS+NR KARLKVAKLHE+IA+QR DFLHKLS ++I +NQ IV+EDL+VK Sbjct: 213 KLQKDLSRKRKGSNNRNKARLKVAKLHERIADQRADFLHKLSIKLIRENQSIVIEDLKVK 272 Query: 236 NMMQNHKLAKAISEVSWSMFREILTYKSKWYGRELIIAPKNFASSQLCSCCGYKNAEVKN 295 NM QNH+LAKAISE SWS FR +L YK++WYGREL+IAP N+ASSQLCS CGYKN EVKN Sbjct: 273 NMQQNHRLAKAISEASWSEFRRMLEYKAEWYGRELVIAPSNYASSQLCSECGYKNEEVKN 332 Query: 296 LGVRQWTCPECTTLHDRDVNAAKNLLKLAM 325 L +R+WTCP+C+ HDRD+NA+KNLLKLAM Sbjct: 333 LALREWTCPQCSAHHDRDINASKNLLKLAM 362 >B1INY3.1 B1INY3_CLOBK Transposase Length = 362 Score = 431 bits (1107), Expect = e-119, Method: Compositional matrix adjust. Identities = 211/330 (63%), Positives = 253/330 (76%), Gaps = 5/330 (1%) Query: 1 MLADKIEHYKQTETM-----LNNTPAQYKKDFDWLKEVDSLALANAQLNLDKAYKNFFRD 55 MLAD+I+ YK+ + + +TPAQYKK+F+WLKEVDSLALANAQ+NL+KAYKNFF+D Sbjct: 33 MLADRIKSYKENKDLDIKKVKYSTPAQYKKEFEWLKEVDSLALANAQINLNKAYKNFFKD 92 Query: 56 XXXXXXXXXXXXTNNHSFTTNNQNGTVAIENGXXXXXXXXXXXXXXXHRQLVGLIKSCTI 115 +N ++TTNNQ GT+ IENG HRQL GLIKSCTI Sbjct: 93 KSIGFPKFKSKKSNYRNYTTNNQKGTIFIENGHIKVPKLKTMIRIKQHRQLNGLIKSCTI 152 Query: 116 SKTPTGKYYAAVLVETGVISLPKLESKIGVDLGLKDFAITSHGEIIPNPKYLRTSEKRLV 175 S+ P+GKYY ++LV+T I LPK KIGVD+GLK+FAI S+G PNPK+LR SEKRL Sbjct: 153 SQVPSGKYYISILVDTENIQLPKTNKKIGVDVGLKEFAICSNGYRQPNPKHLRKSEKRLK 212 Query: 176 KLQKDLARKKKGSHNRYKARLKVAKLHEKIANQRKDFLHKLSTQMINDNQVIVMEDLRVK 235 KLQKDL+RK+K S+NR KARLKVA LHEKI NQR DFLHKLS ++I +NQ IV+EDL+VK Sbjct: 213 KLQKDLSRKQKVSNNRKKARLKVANLHEKIVNQRADFLHKLSIKLIRENQSIVIEDLKVK 272 Query: 236 NMMQNHKLAKAISEVSWSMFREILTYKSKWYGRELIIAPKNFASSQLCSCCGYKNAEVKN 295 NM QNHKLAK ISE SWS FR +L YK++WYGRE+IIAP N+ASSQLCS CGYKN EVKN Sbjct: 273 NMQQNHKLAKVISEASWSEFRRMLEYKAEWYGREIIIAPSNYASSQLCSECGYKNPEVKN 332 Query: 296 LGVRQWTCPECTTLHDRDVNAAKNLLKLAM 325 L +R+W CP+C T HDRD+NA+KNLLKLAM Sbjct: 333 LALREWICPQCGTQHDRDLNASKNLLKLAM 362 >C3HBT9.1 C3HBT9_BACTU Transposase, IS605 OrfB Length = 362 Score = 424 bits (1090), Expect = e-117, Method: Compositional matrix adjust. Identities = 205/330 (62%), Positives = 255/330 (77%), Gaps = 5/330 (1%) Query: 1 MLADKIEHYKQTETMLNN-----TPAQYKKDFDWLKEVDSLALANAQLNLDKAYKNFFRD 55 MLAD+I+ Y++++ L+ TPAQYK +F +LKEVDSLALANAQ+NL+KAY NFFRD Sbjct: 33 MLADRIKSYQESQDKLDKSVKYPTPAQYKAEFPFLKEVDSLALANAQMNLNKAYANFFRD 92 Query: 56 XXXXXXXXXXXXTNNHSFTTNNQNGTVAIENGXXXXXXXXXXXXXXXHRQLVGLIKSCTI 115 N+ S+TTNNQ GTV IE+G HRQ GLIKS T+ Sbjct: 93 KSVGFPKFKSKKDNHRSYTTNNQKGTVYIESGYIKLPKLKTLVKIKQHRQFFGLIKSTTV 152 Query: 116 SKTPTGKYYAAVLVETGVISLPKLESKIGVDLGLKDFAITSHGEIIPNPKYLRTSEKRLV 175 S+TPTGKY+ ++LVE PK+++K+G+D+GLKDFAI S+G+ NPK+LR +E+RLV Sbjct: 153 SQTPTGKYFVSILVEENEQLFPKVDAKVGIDVGLKDFAILSNGKKYENPKWLRKAERRLV 212 Query: 176 KLQKDLARKKKGSHNRYKARLKVAKLHEKIANQRKDFLHKLSTQMINDNQVIVMEDLRVK 235 LQ+ L+RKKKGS+NR KARL+VA+LHEKIANQR DFLHK+S ++ N+NQVIV+EDL+VK Sbjct: 213 LLQRSLSRKKKGSNNRNKARLQVARLHEKIANQRNDFLHKISNEITNENQVIVIEDLKVK 272 Query: 236 NMMQNHKLAKAISEVSWSMFREILTYKSKWYGRELIIAPKNFASSQLCSCCGYKNAEVKN 295 NM +NHKLAKAISEVSW+ FRE L YK+ W GR+LIIAPKN+ASSQLCSCCGYKN EVKN Sbjct: 273 NMQKNHKLAKAISEVSWTKFREHLDYKAIWKGRDLIIAPKNYASSQLCSCCGYKNKEVKN 332 Query: 296 LGVRQWTCPECTTLHDRDVNAAKNLLKLAM 325 L +R+WTCPEC + HDRDVNA+ NLLKLAM Sbjct: 333 LSLREWTCPECNSHHDRDVNASINLLKLAM 362 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.131 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,680,638,405 Number of extensions: 60323227 Number of successful extensions: 168338 Number of sequences better than 1.0e-05: 2097 Number of HSP's gapped: 164671 Number of HSP's successfully gapped: 2098 Length of query: 325 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 189 Effective length of database: 1,779,438,163 Effective search space: 336313812807 Effective search space used: 336313812807 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)