BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0459.0011.p (218 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C1F3E0.1 C1F3E0_ACIC5 3-oxoadipate CoA-succinyl transferase, bet... 262 1e-68 B0VIH2.1 B0VIH2_9BACT Acetoacetate:butyrate CoA-transferase beta... 261 3e-68 Q1D5V2.1 Q1D5V2_MYXXD 3-oxoacid CoA-transferase family, subunit B 257 4e-67 A8MJB0.1 A8MJB0_ALKOO 3-oxoacid CoA-transferase, B subunit EC=2.... 257 6e-67 Q1ISI3.1 Q1ISI3_ACIBL Butyryl-CoA:acetate CoA transferase EC=2.8... 256 8e-67 >C1F3E0.1 C1F3E0_ACIC5 3-oxoadipate CoA-succinyl transferase, beta subunit EC=2.8.3.5 Length = 229 Score = 262 bits (670), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 125/215 (58%), Positives = 164/215 (76%), Gaps = 1/215 (0%) Query: 3 NREFIVRRIAQEFKDGDVVNLGIGLPTMAVDYIPDNVHIMLQAENGILGVGPKAAKEQEN 62 RE IVRR+AQE +DG VNLGIG+PT+ +++P + ++LQ+ENG+LGVGP +E+ Sbjct: 15 KRERIVRRVAQELQDGFYVNLGIGMPTLVANHVPAGIEVVLQSENGMLGVGPYPFAGEED 74 Query: 63 IDCIDSGGSYVTTMSGACYFDSALSFSIIRGGHVDITVLGALEVDKNGNLASWMIPGKKV 122 D I++G V+ + G +F SA SF++IRGGH+D+++LGA+EVD+ GNLA+WMIPGK V Sbjct: 75 PDLINAGKETVSELPGTAFFSSADSFAMIRGGHIDLSILGAMEVDEAGNLANWMIPGKMV 134 Query: 123 PGMGGAMDLVVGAKRVIVAMDHVNKNGEPKILEKCKLPLTAVNVVKTIVTEMAVIDVIPG 182 GMGGAMDLV A+RVIVAM+H K+G PKIL C LP+T + VV TI TEMA I V P Sbjct: 135 KGMGGAMDLVASARRVIVAMEHTTKDGRPKILRHCTLPITGLKVVDTIATEMAWIKVTP- 193 Query: 183 TGLMLKEIAPGLSVEDVQKATGAKLLVSPNLKAIA 217 GL+L+EIAPGLSVEDVQ+ T +LL+SP LK +A Sbjct: 194 EGLLLQEIAPGLSVEDVQRVTEPRLLLSPQLKQMA 228 >B0VIH2.1 B0VIH2_9BACT Acetoacetate:butyrate CoA-transferase beta subunit EC=2.8.3.9 Length = 217 Score = 261 bits (667), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 127/209 (60%), Positives = 166/209 (79%), Gaps = 1/209 (0%) Query: 1 MNNREFIVRRIAQEFKDGDVVNLGIGLPTMAVDYIPDNVHIMLQAENGILGVGPKAAKEQ 60 ++ R I RIA+EFKDGD VNLGIGLPT A ++IP+ + ++ Q+ENG+LG GPK + Q Sbjct: 3 LDKRAMIGARIAKEFKDGDYVNLGIGLPTEAANHIPEGISVIFQSENGLLGAGPKPPEGQ 62 Query: 61 ENIDCIDSGGSYVTTMSGACYFDSALSFSIIRGGHVDITVLGALEVDKNGNLASWMIPGK 120 E+ D I++GG Y+T + GA +FDSA SF+IIRGGH+D TVLGAL+VD+ GNLA+W+IPGK Sbjct: 63 EDKDMINAGGGYITCIPGASFFDSATSFAIIRGGHIDATVLGALQVDQEGNLANWLIPGK 122 Query: 121 KVPGMGGAMDLVVGAKRVIVAMDHVNKNGEPKILEKCKLPLTAVNVVKTIVTEMAVIDVI 180 VPGMGGAMDLV GAK+VIVAM+H +K G KIL+KC LPLTA V I+T+MAVI+V Sbjct: 123 MVPGMGGAMDLVTGAKKVIVAMEHCDKYGNSKILKKCTLPLTAKGKVNLIITDMAVIEVC 182 Query: 181 PGTGLMLKEIAPGLSVEDVQKATGAKLLV 209 P GL L+EIA GL+++DV +AT A+L++ Sbjct: 183 P-EGLYLREIAEGLTIDDVVQATEAELII 210 >Q1D5V2.1 Q1D5V2_MYXXD 3-oxoacid CoA-transferase family, subunit B Length = 218 Score = 257 bits (657), Expect = 4e-67, Method: Compositional matrix adjust. Identities = 123/216 (56%), Positives = 166/216 (76%), Gaps = 1/216 (0%) Query: 3 NREFIVRRIAQEFKDGDVVNLGIGLPTMAVDYIPDNVHIMLQAENGILGVGPKAAKEQEN 62 RE I +RIAQE +DG VNLGIG+PT+ +YIP + I+LQ+ENG+LG+GP + QE+ Sbjct: 4 TREQIAQRIAQELQDGFYVNLGIGMPTLVANYIPPGMDIVLQSENGLLGIGPWPVEGQED 63 Query: 63 IDCIDSGGSYVTTMSGACYFDSALSFSIIRGGHVDITVLGALEVDKNGNLASWMIPGKKV 122 D I++G VT + GA +FDSALSF +IRGGH+D+ VLGA+EV + G+LA+WMIPGK + Sbjct: 64 PDLINAGKETVTAVPGAAFFDSALSFGMIRGGHIDMAVLGAMEVSEEGDLANWMIPGKMI 123 Query: 123 PGMGGAMDLVVGAKRVIVAMDHVNKNGEPKILEKCKLPLTAVNVVKTIVTEMAVIDVIPG 182 GMGGAMDL VGA+R+ VAM+H NK+G+PKIL+KC LPLT + + IVT+ A IDV P Sbjct: 124 KGMGGAMDLAVGAQRIFVAMEHANKDGKPKILKKCSLPLTGLKCIHHIVTDHAYIDVTP- 182 Query: 183 TGLMLKEIAPGLSVEDVQKATGAKLLVSPNLKAIAV 218 GL+L+E+APG++VE VQK T KL V+P+++ + V Sbjct: 183 QGLILREVAPGVTVEQVQKLTEPKLTVAPDVREMKV 218 >A8MJB0.1 A8MJB0_ALKOO 3-oxoacid CoA-transferase, B subunit EC=2.8.3.9 Length = 218 Score = 257 bits (656), Expect = 6e-67, Method: Compositional matrix adjust. Identities = 133/217 (61%), Positives = 168/217 (77%), Gaps = 3/217 (1%) Query: 1 MNN-REFIVRRIAQEFKDGDVVNLGIGLPTMAVDYIPDNVHIMLQAENGILGVGPKAAKE 59 MNN +EFI RR+A+E KDGDVVNLGIGLPTM V+YI +V I+LQ+ENG +G+GP Sbjct: 3 MNNPKEFIARRVAKELKDGDVVNLGIGLPTMVVNYIEKDVDIILQSENGFVGLGPAPEAG 62 Query: 60 QENIDCIDSGGSYVTTMSGACYFDSALSFSIIRGGHVDITVLGALEVDKNGNLASWMIPG 119 +EN +++GG VT G +FDSA+SF+IIRGGHVD TVLGAL+VD+ GNLA+WMIPG Sbjct: 63 KENPYIVNAGGQPVTIKEGGVFFDSAMSFAIIRGGHVDATVLGALQVDQEGNLANWMIPG 122 Query: 120 KKVPGMGGAMDLVVGAKRVIVAMDHVNKNGEPKILEKCKLPLTAVNVVKTIVTEMAVIDV 179 K VPGMGGAMDLVVGAK+VIVAM+H K G KIL+KC LPLTA V IVTEM V++V Sbjct: 123 KMVPGMGGAMDLVVGAKKVIVAMEHTAK-GSAKILKKCTLPLTAAAQVDLIVTEMGVMEV 181 Query: 180 IPGTGLMLKEIAPGLSVEDVQKATGAKLLVSPNLKAI 216 I GL+LKEI P ++++V+ AT A L++ +LK++ Sbjct: 182 IK-EGLVLKEINPAFTLDEVKAATEADLIIPADLKSM 217 >Q1ISI3.1 Q1ISI3_ACIBL Butyryl-CoA:acetate CoA transferase EC=2.8.3.8 Length = 229 Score = 256 bits (655), Expect = 8e-67, Method: Compositional matrix adjust. Identities = 123/213 (57%), Positives = 162/213 (76%), Gaps = 1/213 (0%) Query: 4 REFIVRRIAQEFKDGDVVNLGIGLPTMAVDYIPDNVHIMLQAENGILGVGPKAAKEQENI 63 RE I+RR+A+E +DG VNLGIG+PT+ +++P V ++LQ+ENG+LGVGP E+ Sbjct: 16 RERIIRRVARELRDGYYVNLGIGMPTLVANHVPAGVQVVLQSENGMLGVGPYPYPGDEDP 75 Query: 64 DCIDSGGSYVTTMSGACYFDSALSFSIIRGGHVDITVLGALEVDKNGNLASWMIPGKKVP 123 D I++G VT + G YF SA SF++IRGGH+D++VLGA+EVD+ GNLA+WMIPGK V Sbjct: 76 DLINAGKETVTELPGTSYFSSADSFAMIRGGHIDLSVLGAMEVDEEGNLANWMIPGKMVK 135 Query: 124 GMGGAMDLVVGAKRVIVAMDHVNKNGEPKILEKCKLPLTAVNVVKTIVTEMAVIDVIPGT 183 GMGGAMDLV A+RV+VAM+H + G PKIL KC LP+T + VV TIVTEMA I V Sbjct: 136 GMGGAMDLVASARRVVVAMEHTTREGSPKILTKCTLPITGLKVVNTIVTEMAYIRVT-DK 194 Query: 184 GLMLKEIAPGLSVEDVQKATGAKLLVSPNLKAI 216 GL+L+E+APGL+ +DVQKATG KL+VS L+ + Sbjct: 195 GLVLEEVAPGLTADDVQKATGPKLIVSEKLRTM 227 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.137 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,344,994,141 Number of extensions: 57187676 Number of successful extensions: 124965 Number of sequences better than 1.0e-05: 1165 Number of HSP's gapped: 123679 Number of HSP's successfully gapped: 1166 Length of query: 218 Length of database: 3,060,696,203 Length adjustment: 130 Effective length of query: 88 Effective length of database: 1,835,964,253 Effective search space: 161564854264 Effective search space used: 161564854264 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)