BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0459.0009.p (246 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B2IEG3.1 B2IEG3_BEII9 Acetoacetate decarboxylase EC=4.1.1.4 310 6e-83 B6R751.1 B6R751_9RHOB Putative acetoacetate decarboxylase EC=4.1... 306 1e-81 B8ID67.1 B8ID67_METNO Acetoacetate decarboxylase EC=4.1.1.4 291 2e-77 Q22MK2.1 Q22MK2_TETTH Acetoacetate decarboxylase EC=4.1.1.4 274 5e-72 A9VBK8.1 A9VBK8_MONBE Predicted protein 254 6e-66 >B2IEG3.1 B2IEG3_BEII9 Acetoacetate decarboxylase EC=4.1.1.4 Length = 245 Score = 310 bits (795), Expect = 6e-83, Method: Compositional matrix adjust. Identities = 149/246 (60%), Positives = 183/246 (74%), Gaps = 2/246 (0%) Query: 1 MNPKEVLSLQSMPAASASYGRPPYRFIDREFFIITYESDPAAIRQAVPEPLQPDGSNTVS 60 MN E+L+ SMPA SY + PYRFI+RE+ II+YESDP AIR AVPEPL P SN ++ Sbjct: 1 MNRDEILTAASMPAFGPSYPKGPYRFINREYMIISYESDPDAIRAAVPEPLVP-ASNVIT 59 Query: 61 YEWIKMPDSSGLGSYEESGIVIPCTFQGEPCNFVAQMYLDNAPAILGGREIWGFPKKWAN 120 YEW++MPDSSG G Y E+G VIPC G+P +F QMYLD+ I GREIWGFPKK A Sbjct: 60 YEWMRMPDSSGFGDYTETGTVIPCLLDGQPVSFTVQMYLDDEAPIAAGREIWGFPKKHAQ 119 Query: 121 PRLSVEGTETLTGTLHYNHVLVAMGTMPFKHNLLDQADTAAAIAKTQVNLKLIPDVDGSP 180 P+L + T+TLTGTL+Y + VA+GTM +K LD+A A+ K QV LKLIPDVDG P Sbjct: 120 PKLELV-TDTLTGTLYYANERVALGTMVYKQQTLDEATALRALGKDQVCLKLIPDVDGKP 178 Query: 181 KIAQLVAYSLENVTVKGAWSGPARLNLIPHVNAPVADLPVKAYIGGKHFIADLTLPYARV 240 KIAQLV +++ ++ KGAW GPARL+LIPHVNAPVADLPV GG H +AD+TLPY +V Sbjct: 179 KIAQLVKFAMTDIAFKGAWQGPARLHLIPHVNAPVADLPVLRTRGGIHLLADMTLPYGKV 238 Query: 241 IHDYLQ 246 +HDYL+ Sbjct: 239 VHDYLE 244 >B6R751.1 B6R751_9RHOB Putative acetoacetate decarboxylase EC=4.1.1.4 Length = 256 Score = 306 bits (783), Expect = 1e-81, Method: Compositional matrix adjust. Identities = 154/256 (60%), Positives = 182/256 (71%), Gaps = 11/256 (4%) Query: 1 MNPKEVLSLQSMPAASASYGRPPYRFIDREFFIITYESDPAAIRQAVPEPLQPDGSNTVS 60 M ++L SMP AS +Y + PYRFIDREFF+++YESDP AIR+A+PEPL+PDGSN V Sbjct: 1 MKIADILEQASMPIASPAYPKGPYRFIDREFFVVSYESDPDAIREALPEPLEPDGSNKVM 60 Query: 61 YEWIKMPDSSGLGSYEESGIVIPCTFQGEPCNFVAQMYLDNAPAILGGREIWGFPKKWAN 120 +E I+MPDS+G G Y ESGIVIP F P NF QM+LD+ P I GGREIWGFPKK + Sbjct: 61 FEVIRMPDSAGFGDYTESGIVIPAKFGDHPVNFTQQMFLDDTPPIFGGREIWGFPKKLGH 120 Query: 121 PRLSVEGTETLTGTLHYNHVLVAMGTMPFK--HNLLDQADTAAA--------IAKTQVNL 170 P LSV G TLTG L ++ V +A+ TM +K H L D + KTQVNL Sbjct: 121 PELSVTGA-TLTGHLEFDGVTIAIATMGYKYQHLLYDVEHGTQCHPDAILKKLQKTQVNL 179 Query: 171 KLIPDVDGSPKIAQLVAYSLENVTVKGAWSGPARLNLIPHVNAPVADLPVKAYIGGKHFI 230 KLIPDVDGS IAQLVAY + +VTVKGAWSGPA L L H NAPVADLP++ +GG HFI Sbjct: 180 KLIPDVDGSLAIAQLVAYEVADVTVKGAWSGPASLQLFHHANAPVADLPMRKSLGGVHFI 239 Query: 231 ADLTLPYARVIHDYLQ 246 DLTLPY RVIHDYL+ Sbjct: 240 TDLTLPYGRVIHDYLK 255 >B8ID67.1 B8ID67_METNO Acetoacetate decarboxylase EC=4.1.1.4 Length = 247 Score = 291 bits (746), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 142/247 (57%), Positives = 171/247 (69%), Gaps = 3/247 (1%) Query: 1 MNPKEVLSLQSMPAASASYGRPPYRFIDREFFIITYESDPAAIRQAVPEPLQPDGSNTVS 60 M E+L SMP S SY PYRFI RE+ IITYE+DP A+R A+PEPL+P N Sbjct: 1 MTRDEILRSPSMPVFSPSYPHGPYRFIRREYLIITYETDPDALRAALPEPLEPAPGNLAF 60 Query: 61 YEWIKMPDSSGLGSYEESGIVIPCTFQGEPCNFVAQMYLDNAPAILGGREIWGFPKKWAN 120 YEW+KMPDSSG G YEESG I + GEPCNF QMYLD+ P I GREIWGFPKK+ Sbjct: 61 YEWMKMPDSSGFGDYEESGTGIVARYNGEPCNFSVQMYLDDEPPITAGREIWGFPKKYGV 120 Query: 121 PRLSVEGTETLTGTLHYNHVLVAMGTMPFKHNLL--DQADTAAAIAKTQVNLKLIPDVDG 178 PRL V +TLTGTLHY+ VA+GTM +KH+ L + I + VNLKL+PDVDG Sbjct: 121 PRLKVM-KDTLTGTLHYDEERVALGTMTYKHHSLAGELDRVREGIGQLNVNLKLLPDVDG 179 Query: 179 SPKIAQLVAYSLENVTVKGAWSGPARLNLIPHVNAPVADLPVKAYIGGKHFIADLTLPYA 238 K+AQL+ Y L+++TV GAW G ARL+LIPH N VADLPV+ + G+H + D TLPY Sbjct: 180 GVKVAQLIGYRLQDITVHGAWEGDARLHLIPHANCRVADLPVRRIVRGRHQVVDFTLPYG 239 Query: 239 RVIHDYL 245 RV+HDYL Sbjct: 240 RVLHDYL 246 >Q22MK2.1 Q22MK2_TETTH Acetoacetate decarboxylase EC=4.1.1.4 Length = 553 Score = 274 bits (700), Expect = 5e-72, Method: Compositional matrix adjust. Identities = 131/246 (53%), Positives = 170/246 (69%), Gaps = 2/246 (0%) Query: 1 MNPKEVLSLQSMPAASASYGRPPYRFIDREFFIITYESDPAAIRQAVPEPLQPDGSNTVS 60 + +E+L L S PA S S+ PYRFIDRE+ II+YE+D +R+ VP PL P SN V Sbjct: 35 LKREEILKLPSQPAISPSFPFGPYRFIDREYLIISYETDIEVLRKVVPYPLVPQ-SNVVL 93 Query: 61 YEWIKMPDSSGLGSYEESGIVIPCTFQGEPCNFVAQMYLDNAPAILGGREIWGFPKKWAN 120 YEWI MPDSSG GSY E+G VIPC GEP NF QMYL+N I GREIWGFPKK+A Sbjct: 94 YEWINMPDSSGFGSYSETGTVIPCLLNGEPVNFTLQMYLNNEAPIASGREIWGFPKKYAK 153 Query: 121 PRLSVEGTETLTGTLHYNHVLVAMGTMPFKHNLLDQADTAAAIAKTQVNLKLIPDVDGSP 180 P + + +TL G L+Y+ +A GTM +K+ + +A+ ++ K Q LK IPDVD SP Sbjct: 154 PSMGI-NKDTLVGRLNYSGEQIAFGTMTYKYKKIPEAEALKSLTKLQCVLKYIPDVDWSP 212 Query: 181 KIAQLVAYSLENVTVKGAWSGPARLNLIPHVNAPVADLPVKAYIGGKHFIADLTLPYARV 240 KIAQL+ ++L ++ +K AW GPA+L LIPHVNAP LPV +GGKH AD+TLP+ +V Sbjct: 213 KIAQLIGFNLCDIQLKEAWEGPAKLELIPHVNAPTTLLPVLKVLGGKHIKADITLPFGKV 272 Query: 241 IHDYLQ 246 +HDY++ Sbjct: 273 LHDYVE 278 Score = 155 bits (392), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 89/243 (36%), Positives = 131/243 (53%), Gaps = 3/243 (1%) Query: 4 KEVLSLQSMPAASASYGRPPYRFIDREFFIITYESDPAAIRQAVPEPLQPDGSNTVSYEW 63 ++VL SMP + S+ + P DRE+ I YES P AI +A+PEPL P N V Y + Sbjct: 306 EDVLRAASMPLIAPSFSKGPLIMDDREYLIFQYESTPEAIARALPEPLIPYDHNYV-YLY 364 Query: 64 IKMPDSSGLGSYEESGIVIPCTFQGEPCNFVAQMYLDNAPAILGGREIWGFPKKWANPRL 123 SGLG + + + +PC E F Q YL+++ + GRE++G P+K+ + + Sbjct: 365 FINASGSGLGEFHKLDMYVPCYLGNELVMFGVQSYLNSSASRTAGREVFGQPQKFGDAEI 424 Query: 124 SVEGTETLTGTLHYNHVLVAMGTMPFKHNLLDQADTAAAIAKTQVNLKLIPD-VDGSPKI 182 +V +TL TL Y+ +A TM +K N LD + VNLK IP V+G I Sbjct: 425 TV-CRDTLKATLKYSDETIAFATMSYKFNKLDPHKAREMLEIPMVNLKFIPSAVEGKVDI 483 Query: 183 AQLVAYSLENVTVKGAWSGPARLNLIPHVNAPVADLPVKAYIGGKHFIADLTLPYARVIH 242 AQL ++ V ++ P +++L+ HVNAP+ DLPV + G H AD + A V + Sbjct: 484 AQLTKMEYSDIHVTASYHSPCQVSLVHHVNAPLKDLPVVKTLDGYHIKADTIIKKATVAY 543 Query: 243 DYL 245 DYL Sbjct: 544 DYL 546 >A9VBK8.1 A9VBK8_MONBE Predicted protein Length = 523 Score = 254 bits (648), Expect = 6e-66, Method: Compositional matrix adjust. Identities = 124/240 (51%), Positives = 159/240 (66%), Gaps = 2/240 (0%) Query: 5 EVLSLQSMPAASASYGRPPYRFIDREFFIITYESDPAAIRQAVPEPLQPDGSNTVSYEWI 64 +++ L S PA S S+ PYRF++RE+ +I YE+D +++ VP PL+P SN V YEWI Sbjct: 25 DIVGLPSAPAISPSFPLGPYRFVNREYCLIEYETDLDRLKKVVPWPLKPT-SNRVIYEWI 83 Query: 65 KMPDSSGLGSYEESGIVIPCTFQGEPCNFVAQMYLDNAPAILGGREIWGFPKKWANPRLS 124 MPDSSG GSY ESG ++PC G+ N+ MYLD+ P GREIWGFPK+ PRL Sbjct: 84 NMPDSSGFGSYSESGCIVPCELNGQSVNYTLSMYLDDEPPTAAGREIWGFPKRHGLPRLK 143 Query: 125 VEGTETLTGTLHYNHVLVAMGTMPFKHNLLDQADTAAAIAKTQVNLKLIPDVDGSPKIAQ 184 VE +TLTG+L Y A+GTM +KH L + + KT NL+LIPDV+G AQ Sbjct: 144 VE-KDTLTGSLTYVGQEAALGTMVYKHTPLSEEKVREKLCKTTCNLRLIPDVNGGLARAQ 202 Query: 185 LVAYSLENVTVKGAWSGPARLNLIPHVNAPVADLPVKAYIGGKHFIADLTLPYARVIHDY 244 LV + LE+VTV AWSGPARL+L+PH P ADL V+ IGG H DLTLP+ RV++DY Sbjct: 203 LVGFELEDVTVHEAWSGPARLHLVPHATTPAADLTVRRVIGGTHVRTDLTLPHGRVLYDY 262 Score = 155 bits (392), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 90/248 (36%), Positives = 137/248 (55%), Gaps = 5/248 (2%) Query: 1 MNPKEVLSLQSMPAASASYGRPPYRFIDREFFIITYESDPAAIRQAVPEPLQPDGSNTVS 60 ++P E+L SMP + SY R +RE+ +++D +RQ +PE + + + Sbjct: 277 LHPAEILKAPSMPIMAPSYPRKFSTLRNREYVFFKFKTDVEILRQHLPELCEVNPEGDIY 336 Query: 61 YEWIKMPDSSGLGSYEESGIVIPCTFQGEPCNFVAQMYLDNAPAILGGREIWGFPKKWAN 120 + WI + GLG+Y + I IPCTF+G+P F LD+ A+ GREI G P K+ Sbjct: 337 FAWIST-EGHGLGAYNKCDIKIPCTFKGKPYLFSVLAVLDSGVALNAGREILGQPCKYGF 395 Query: 121 PRLSVEGTETLTGTLHYNHVLVAMGTMPFKHNLLDQADTAAAIAKTQVNLKLIPDVDGSP 180 P ++VE +TL TL + LVA GT FK+ D + + ++NLK IP VDG Sbjct: 396 PTVAVE-KDTLACTLRFGEQLVATGTSVFKYQRADSDEARDLLTTPELNLKFIPGVDGRA 454 Query: 181 KIAQLVAYSLENVTVKGA--WSGPARLNLIPHVNAPVADLPVKAYIGGKHF-IADLTLPY 237 IAQLV+ +V + + ++G RL L PHVNAP+AD PV++ H + +++ Sbjct: 455 DIAQLVSVKHGDVHLDDSLLYTGKGRLALRPHVNAPLADFPVQSDSQVLHVRVNSMSIKQ 514 Query: 238 ARVIHDYL 245 +V+HDYL Sbjct: 515 TQVVHDYL 522 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,596,054,491 Number of extensions: 68252546 Number of successful extensions: 121803 Number of sequences better than 1.0e-05: 127 Number of HSP's gapped: 121545 Number of HSP's successfully gapped: 129 Length of query: 246 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 114 Effective length of database: 1,817,122,223 Effective search space: 207151933422 Effective search space used: 207151933422 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)