BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0454.0016.p (468 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A6LZV2.1 A6LZV2_CLOB8 Glycoside hydrolase, family 3 domain protein 632 e-179 B8HZZ6.1 B8HZZ6_CLOCE Glycoside hydrolase family 3 domain protein 419 e-115 Q0UWV6.1 Q0UWV6_PHANO Putative uncharacterized protein 303 3e-80 B8MWY2.1 B8MWY2_ASPFN Periplasmic beta-glucosidase, putative 292 4e-77 Q2USQ4.1 Q2USQ4_ASPOR Beta-glucosidase-related glycosidases 292 6e-77 >A6LZV2.1 A6LZV2_CLOB8 Glycoside hydrolase, family 3 domain protein Length = 689 Score = 632 bits (1630), Expect = e-179, Method: Compositional matrix adjust. Identities = 295/470 (62%), Positives = 371/470 (78%), Gaps = 3/470 (0%) Query: 1 LYHGVHCGHHDYLLNQVLKDEWDFDGFVISDFAWGIKDTVEAANGGMDVEMCHTKFFGGK 60 LY G HCGHHDYLLNQVLK EWDFDGFV+SDF WG++DTVEAANGG D+EMC T+FFG K Sbjct: 220 LYKGTHCGHHDYLLNQVLKKEWDFDGFVMSDFIWGVRDTVEAANGGQDMEMCCTQFFGDK 279 Query: 61 LVEAVRNGQVSEEKIDESAVRIVRTLLAFTEADKDTYGKELIASKEHIALALEAAEKSMT 120 LVEAV+NGQV E KIDESA+RIVRTLLAF +A + Y + +I ++HI LAL+AA + +T Sbjct: 280 LVEAVKNGQVKESKIDESALRIVRTLLAFEDAYSNEYDESVIGCEDHIKLALQAAREGIT 339 Query: 121 LMKNDHHVLPFSKTETKRIAVIGELGSKGNIGDHGSSRVFPPYIVTPLEGIKRLLPNAEV 180 L+KN+++VLP SK + K+I V+G+LG K IGDHGSS+V P Y++TPL+GI P A+V Sbjct: 340 LIKNENNVLPLSKDKAKKIVVLGKLGDKEVIGDHGSSQVRPAYVITPLQGIANSAPKAQV 399 Query: 181 IFDSGEDLEQAKQLAKSVDAVVFVAGYDHDDEGESVTNEDADL---KAGGDRKNSLGLHT 237 ++ +GE+LE AK+LAK DAV+FV GY++DDEGE ++ ++ + GGDRKNSLGLH Sbjct: 400 VYYNGENLEHAKELAKDADAVIFVVGYNYDDEGEYISEDEFESYTGAVGGDRKNSLGLHE 459 Query: 238 KDIELIKAVGPVNPNSAAVLIGGNMIMIEEWKQSVSSILMAYYPGMEGGTAIAKTLFGDV 297 +I+LI+ VGPVN NS AVLIGGNMIM+EEWK+SV +I+MAYYPGMEGGTAI + +FGDV Sbjct: 460 NEIKLIQEVGPVNTNSIAVLIGGNMIMMEEWKESVGAIMMAYYPGMEGGTAIGEIIFGDV 519 Query: 298 NPGGKLPFVVPVSESHLPQVDWDATEIKYDYYHGYAKLEKEGIEPSLPYGYGLSYTSFAL 357 NP GKLP+V+P ES LPQV+WD T YDYYHGY KLEKEGI+PS PYGYGLSYT F Sbjct: 520 NPSGKLPYVIPFKESDLPQVNWDTTSQWYDYYHGYTKLEKEGIKPSAPYGYGLSYTKFDF 579 Query: 358 SNASFKTVDDHIIAACEVENTGDVTGDEVVQLYVGFGNSGLDRPVKVLRGFKRITLKPGQ 417 S+A F DD +IA C V+N G + GDEVVQ+YVGF NS ++RPVK+LRGF R+ L+PG+ Sbjct: 580 SDAEFDASDDSVIAKCTVKNIGKMAGDEVVQMYVGFKNSSVERPVKLLRGFARVNLQPGE 639 Query: 418 KEKVEIACPVEKIKWFNPAANRWELEEMAYEVYIGTSSAEKHLIKGSINL 467 ++V I CP E++ W+NP + ELE+M YEVYIG+SSA+ L G + L Sbjct: 640 SKEVTITCPKEELCWYNPKTEQMELEKMEYEVYIGSSSADSDLHIGKVTL 689 >B8HZZ6.1 B8HZZ6_CLOCE Glycoside hydrolase family 3 domain protein Length = 724 Score = 419 bits (1077), Expect = e-115, Method: Compositional matrix adjust. Identities = 222/468 (47%), Positives = 297/468 (63%), Gaps = 8/468 (1%) Query: 3 HGVHCGHHDYLLNQVLKDEWDFDGFVISDFAWGIKDTVEAANGGMDVEMCHTKFFGGKLV 62 G CGHHDYLLNQ+L EW F GFV+SDF WGI+DTV+AANGG +EM T+FFG +LV Sbjct: 261 RGEWCGHHDYLLNQILNGEWGFKGFVMSDFIWGIRDTVDAANGGQCMEMYATQFFGKRLV 320 Query: 63 EAVRNGQVSEEKIDESAVRIVRTLLAFT-EADKDTYGKELIASKEHIALALEAAEKSMTL 121 +AVR+G+V E KID + RI+R + F+ DK Y +E + KEH LA E +EKS L Sbjct: 321 DAVRDGKVDEIKIDAAVKRILREKIRFSFVGDKAQYSEEKMGCKEHAELAREVSEKSTIL 380 Query: 122 MKNDHHVLPFSKTETKRIAVIGELGSKGNIGD-HGSSRVFPPYIVTPLEGI-KRLLPNAE 179 +KN+ +LP K + K+I V+G L + N GD GSS VFPPY++TPL+GI E Sbjct: 381 LKNEG-LLPLEKDKIKKITVVGRLAVEPNTGDLKGSSAVFPPYVITPLQGILNEAGSGIE 439 Query: 180 VIFDSGEDLEQAKQLAKSVDAVVFVAGYDHDDEGESVTNEDADLKAGGDRKNSLGLHTKD 239 + + G QA++L+K D VV +AG DEGE +TN GGDRK LGLH +D Sbjct: 440 IKYADGRIPHQAQELSKDADVVVIIAGLTSLDEGEFITNGG---DVGGDRK-YLGLHPED 495 Query: 240 IELIKAVGPVNPNSAAVLIGGNMIMIEEWKQSVSSILMAYYPGMEGGTAIAKTLFGDVNP 299 +LI+AV N N VL GG+ I++E WK +IL+ +YPGMEGGTA+A LFG NP Sbjct: 496 EDLIEAVSKANKNVIVVLQGGSTIIVEPWKNLAKAILIQWYPGMEGGTALANILFGKANP 555 Query: 300 GGKLPFVVPVSESHLPQVDWDATEIKYDYYHGYAKLEKEGIEPSLPYGYGLSYTSFALSN 359 KLP VP + LP D +AT I YDYYHGY +KE S P+G+GLSYT+++ SN Sbjct: 556 SAKLPITVPATPQQLPYFDMNATSIAYDYYHGYFLADKEKYPVSYPFGFGLSYTNYSYSN 615 Query: 360 ASFKTVDDHIIAACEVENTGDVTGDEVVQLYVGFGNSGLDRPVKVLRGFKRITLKPGQKE 419 D I + ++ NTG + GDEVVQLY+G+ S ++R VK L+GF +I L+PG+K+ Sbjct: 616 PKVTKNGDTITVSVDISNTGKIAGDEVVQLYIGYKGSKVERHVKDLKGFTKIHLEPGEKK 675 Query: 420 KVEIACPVEKIKWFNPAANRWELEEMAYEVYIGTSSAEKHLIKGSINL 467 I E + ++ P + +W +E++ Y IG SSA K L+K +L Sbjct: 676 TAIIEIDKEDLAYYCPESKQWVVEDIGYVALIGASSACKDLLKADFDL 723 >Q0UWV6.1 Q0UWV6_PHANO Putative uncharacterized protein Length = 740 Score = 303 bits (775), Expect = 3e-80, Method: Compositional matrix adjust. Identities = 176/496 (35%), Positives = 261/496 (52%), Gaps = 52/496 (10%) Query: 3 HGVHCGHHDYLLNQVLKDEWDFDGFVISDFAWGIKDTVEAANGGMDVEMCHTKFFGGKLV 62 +G CG LL ++L++ W FDGF +SDF +G +D + G+D+E ++ L Sbjct: 230 NGEWCGQSRKLLTEILRETWKFDGFTMSDFVFGFRDAPLSVKNGLDIEAPFSQQRAMHLE 289 Query: 63 EAVRNGQVSEEKIDESAVRIVRTLLAFTEADKDTY-GKELIASKEHIALALEAAEKSMTL 121 +A++ G++ E ++++ RI+ L F K K ++ EH ALA EAA++SM L Sbjct: 290 KALQTGELDWEDVEKACTRILSKQLDFVAGLKTPKPDKSVVFCDEHRALAREAAQRSMVL 349 Query: 122 MKNDH----HVLPFSKTETKRIAVIGELGSKGNIGDHGSSRVFPPYIVTPLEGIKRLLPN 177 +KN++ +LP IA++G L + NIGDHGSS+V PP++ TP EG+K LP+ Sbjct: 350 LKNEYVGNSAILPLQGPNIGSIALVGRLANVANIGDHGSSKVEPPHVATPYEGLKTALPS 409 Query: 178 AEVIFDSGEDLEQAKQLAKSVDAVVFVAGYDHDDEGESV---------------TNEDAD 222 A ++ + + E+AK A D + + GYDH DEGE + T ED D Sbjct: 410 ASIVLEGTDSPEKAKDAASKADVAICIVGYDHHDEGEHMLPNHDAGVASLFPPPTPEDGD 469 Query: 223 LK--------------------------AGGDRKNSLGLHTKDIELIKAVGPVNPNSAAV 256 + +GGDRK SL L D+ LI+AV NP + V Sbjct: 470 VSQIIGVLNGEAKPSAALTQEGSIIEAGSGGDRK-SLRLRPNDVSLIEAVTAANPRTIVV 528 Query: 257 LIGGNMIMIEEWKQSVSSILMAYYPGMEGGTAIAKTLFGDVNPGGKLPFVVPVSESHLPQ 316 ++ ++IEEWK V +ILM +Y G EGG A+A L G NP G+LPF VP SE HLP Sbjct: 529 IVAAGAVIIEEWKDKVPAILMGWYAGSEGGHALADVLLGRTNPSGRLPFSVPKSEEHLPY 588 Query: 317 VDWDATEIKYDYYHGYAKLEKEGIEPSLPYGYGLSYTSFALSNASFKTVD---DHIIAAC 373 D +A +I YD +HG L+K G+E + P G+GLSYT+FA+S+ T D + I Sbjct: 589 FDMNAKKITYDRWHGQHLLDKLGVEAAFPSGFGLSYTTFAISDLRVGTPDLGNESIDVKV 648 Query: 374 EVENTGDVTGDEVVQLYVGFGNSGLDRPVKVLRGFKRITLKPGQKEKVEIACPVEKIKWF 433 V+NTG G + Q+Y G D P +VL GF + L+ G+ + I+ + ++ + Sbjct: 649 SVKNTGGRAGRFIAQVY-GLSEQP-DFPKRVLLGFAPVDLEAGESKTASISASLRPLQRW 706 Query: 434 NPAANRWELEEMAYEV 449 E E+ EV Sbjct: 707 KEGKFSLETGEVTVEV 722 >B8MWY2.1 B8MWY2_ASPFN Periplasmic beta-glucosidase, putative Length = 726 Score = 292 bits (748), Expect = 4e-77, Method: Compositional matrix adjust. Identities = 174/492 (35%), Positives = 264/492 (53%), Gaps = 52/492 (10%) Query: 3 HGVHCGHHDYLLNQVLKDEWDFDGFVISDFAWGIKDTVEAANGGMDVEMCHTKFFGGKLV 62 +G G + +LL ++L+D+W FDG V+SDF +G++D + G+D+E + KL Sbjct: 226 NGEWAGQNRHLLTEILRDQWGFDGLVMSDFIFGLRDAAASVKNGLDIEAPFRQQRARKLP 285 Query: 63 EAVRNGQVSEEKIDESAVRIVRTLLAFTEADKDTY-GKELIASKEHIALALEAAEKSMTL 121 A+ +G++ + +D + RI+R + FT +D+ ++++ EH ALA E A +SM L Sbjct: 286 RALESGELDWKYVDRACERILRKQIEFTVRTEDSQPSRDVVFCDEHRALAREVAARSMVL 345 Query: 122 MKN----DHHVLPFSKTETKRIAVIGELGSKGNIGDHGSSRVFPPYIVTPLEGIKRLLPN 177 +KN D VLP R+AV+G L + N GD GSS+VFPP +VTPL+GIK LP Sbjct: 346 LKNDTVDDKAVLPLQAESLSRVAVVGRLANIANTGDKGSSQVFPPGVVTPLDGIKAALPG 405 Query: 178 AEVIFDSGEDLEQAKQLAKSVDAVVFVAGYDHDDEGESVT-------------------- 217 EV+F + + +A+QLA VD V+ + GY H+DEGE V Sbjct: 406 TEVLF--ADSVAKAEQLASQVDVVICIVGYTHEDEGEYVVPALQDNPALRDTLPPATTAE 463 Query: 218 -NEDADL---------------KAGGDRKNSLGLHTKDIELIKAVGPVNPNSAAVLIGGN 261 E D+ AGGDR NSL L +D +LI AV NP + +I Sbjct: 464 ERETLDIFEGNSDKGGNGGIKAGAGGDR-NSLRLREEDEKLISAVTAHNPRTIVSVITAG 522 Query: 262 MIMIEEWKQSVSSILMAYYPGMEGGTAIAKTLFGDVNPGGKLPFVVPVSESHLPQVDWDA 321 +++E WK V ++L+++Y G EGG + L G V+ G+LPF +P SE++LP + +A Sbjct: 523 AVIMESWKDRVPALLISWYSGSEGGHGLGDVLLGKVDASGRLPFSIPTSEAYLPFFNRNA 582 Query: 322 TEIKYDYYHGYAKLEKEGIEPSLPYGYGLSYTSFALSNASFKTVD-DHIIAACEVENTGD 380 EI YD + G L+K G++ P G+GLSYT+FA+ N ++VD + I A V+NTG Sbjct: 583 AEIYYDRWFGQHMLDKLGVKAEFPLGFGLSYTTFAVDNIIAESVDKESIQVAVNVQNTGR 642 Query: 381 VTGDEVVQLYVGFGNSGLDRPVKVLRGFKRITLKPGQKEKVEIACPVEKI-KW----FNP 435 G + Q Y + D P +VL GF + L GQK K+ + +W F Sbjct: 643 RPGRFIAQAYA--VTNIPDFPTRVLLGFAPVDLDVGQKTKMRFLASTRPLQQWKAGTFTL 700 Query: 436 AANRWELEEMAY 447 N+ +L+ ++ Sbjct: 701 RTNQIQLQVASF 712 >Q2USQ4.1 Q2USQ4_ASPOR Beta-glucosidase-related glycosidases Length = 726 Score = 292 bits (747), Expect = 6e-77, Method: Compositional matrix adjust. Identities = 174/492 (35%), Positives = 264/492 (53%), Gaps = 52/492 (10%) Query: 3 HGVHCGHHDYLLNQVLKDEWDFDGFVISDFAWGIKDTVEAANGGMDVEMCHTKFFGGKLV 62 +G G + +LL ++L+D+W FDG V+SDF +G++D + G+D+E + KL Sbjct: 226 NGEWAGQNRHLLTEILRDQWGFDGLVMSDFIFGLRDAAASVKNGLDIEAPFRQQRARKLP 285 Query: 63 EAVRNGQVSEEKIDESAVRIVRTLLAFTEADKDTY-GKELIASKEHIALALEAAEKSMTL 121 A+ +G++ + +D + RI+R + FT +D+ ++++ EH ALA E A +SM L Sbjct: 286 RALESGELDWKYVDRACERILRKQIEFTVRTEDSQPSRDVVFCDEHRALAREVAARSMVL 345 Query: 122 MKND----HHVLPFSKTETKRIAVIGELGSKGNIGDHGSSRVFPPYIVTPLEGIKRLLPN 177 +KND VLP R+AV+G L + N GD GSS+VFPP +VTPL+GIK LP Sbjct: 346 LKNDTVDGKAVLPLQAESLSRVAVVGRLANIANTGDKGSSQVFPPGVVTPLDGIKAALPG 405 Query: 178 AEVIFDSGEDLEQAKQLAKSVDAVVFVAGYDHDDEGESVT-------------------- 217 EV+F + + +A+QLA VD V+ + GY H+DEGE V Sbjct: 406 TEVLF--ADSVAKAEQLASQVDVVICIVGYTHEDEGEYVVPALQDNPALRDTLPPATTAE 463 Query: 218 -NEDADL---------------KAGGDRKNSLGLHTKDIELIKAVGPVNPNSAAVLIGGN 261 E D+ AGGDR NSL L +D +LI AV NP + +I Sbjct: 464 ERETLDIFEGNSDKGGNGGIKAGAGGDR-NSLRLREEDEKLIPAVTAHNPRTIVSVITAG 522 Query: 262 MIMIEEWKQSVSSILMAYYPGMEGGTAIAKTLFGDVNPGGKLPFVVPVSESHLPQVDWDA 321 +++E WK V ++L+++Y G EGG + L G V+ G+LPF +P SE++LP + +A Sbjct: 523 AVIMESWKDRVPALLISWYSGSEGGHGLGDVLLGKVDASGRLPFSIPTSEAYLPFFNRNA 582 Query: 322 TEIKYDYYHGYAKLEKEGIEPSLPYGYGLSYTSFALSNASFKTVD-DHIIAACEVENTGD 380 EI YD + G L+K G++ P G+GLSYT+FA+ N ++VD + I A V+NTG Sbjct: 583 AEIYYDRWFGQHMLDKLGVKAEFPLGFGLSYTTFAVDNIIAESVDKESIQVAVNVQNTGR 642 Query: 381 VTGDEVVQLYVGFGNSGLDRPVKVLRGFKRITLKPGQKEKVEIACPVEKI-KW----FNP 435 G + Q Y + D P +VL GF + L GQK K+ + +W F Sbjct: 643 RPGRFIAQAYA--VTNIPDFPTRVLLGFAPVDLDVGQKTKMRFLASTRPLQQWKAGTFTL 700 Query: 436 AANRWELEEMAY 447 N+ +L+ ++ Sbjct: 701 RTNQIQLQVASF 712 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.136 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,999,974,262 Number of extensions: 131065104 Number of successful extensions: 246390 Number of sequences better than 1.0e-05: 1870 Number of HSP's gapped: 239603 Number of HSP's successfully gapped: 2289 Length of query: 468 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 329 Effective length of database: 1,751,175,118 Effective search space: 576136613822 Effective search space used: 576136613822 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 133 (55.8 bits)