BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0454.0007.p (313 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q2BFJ8.1 Q2BFJ8_9BACI Putative transport system permease ABC tra... 289 2e-76 Q9KDU3.1 Q9KDU3_BACHD Lactose transport system (Permease) 286 2e-75 C5C4L6.1 C5C4L6_9MICO Binding-protein-dependent transport system... 286 2e-75 Q5WL27.1 Q5WL27_BACSK Lactose ABC transporter permease 283 2e-74 B3KAH3.1 B3KAH3_9BACI Binding-protein-dependent transport system... 279 3e-73 >Q2BFJ8.1 Q2BFJ8_9BACI Putative transport system permease ABC transporter protein Length = 323 Score = 289 bits (740), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 150/303 (49%), Positives = 206/303 (67%), Gaps = 5/303 (1%) Query: 7 RPAVTVKPTKKAGKSKNVLIAYLFLLPWLIGLFVLTLWPFINSFYXXXXXXXXXXX-XFV 65 + V ++ K++ + KN + Y FL PWLIGL L++ P S Y ++ Sbjct: 23 KEGVCMQSLKRSWQ-KN-WVGYFFLTPWLIGLIGLSVIPMAASLYFSFTSYDMFTAPEWI 80 Query: 66 GLQNYIDMLSDARFWKSMEVTLKYVVLSVPLKLAMALFVAILLYKASLGMTIYRTLFYLP 125 GL NYI+M +DA++ KS++VTL YV L VPL+LA AL VA+LL + G+ +YR ++Y+P Sbjct: 81 GLSNYIEMFNDAKWLKSVKVTLIYVFLGVPLQLAFALGVAVLLNRGLKGLQVYRAIYYVP 140 Query: 126 SLIGGSVAVAVMWRNIFGMEGLLNSFLSLMGITGKEWLGQPDYALYVLILLVVWQFGSSM 185 SL GGSVA+A++WR +FG EGL+N L+L+GI G+ W+ PD ALY LI+L +WQFG+ M Sbjct: 141 SLFGGSVAIALLWRQLFGGEGLINKVLALVGIEGQNWISSPDTALYTLIILTIWQFGAPM 200 Query: 186 VIFLAGLKNVPGDLYEAAQIDGAGSVARFFKITLPMISPILLFNLILQTISSFQVFTQGY 245 VIFLAGLK VP +LYE++ IDGAG V +FF ITLP+I+PI+ FNL++Q IS+FQ FT Y Sbjct: 201 VIFLAGLKQVPVELYESSLIDGAGPVKQFFSITLPLITPIIFFNLVMQIISAFQAFTPAY 260 Query: 246 IIT--KGGPMDETLFSVLYIYDLAFKQQRMGYASAVSWXXXXXXXXXXXXXXXXSKRWVY 303 I++ KGGP+D TLF LY+Y F Q +MGYASA++W SK+WVY Sbjct: 261 IVSGGKGGPLDSTLFYTLYLYQKGFTQFQMGYASAMAWFLLACISVVTAIVFISSKKWVY 320 Query: 304 YEN 306 Y+ Sbjct: 321 YQE 323 >Q9KDU3.1 Q9KDU3_BACHD Lactose transport system (Permease) Length = 318 Score = 286 bits (732), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 145/297 (48%), Positives = 196/297 (65%), Gaps = 2/297 (0%) Query: 13 KPTKKAGKSKNVLIAYLFLLPWLIGLFVLTLWPFINSFYXXXXXX-XXXXXXFVGLQNYI 71 K ++ K K + Y + PWL+G F L + P I S Y +VGLQNYI Sbjct: 18 KTRQERKKWKESFVGYAMISPWLLGFFGLVIGPMIASLYFSFTDYDMLSSANWVGLQNYI 77 Query: 72 DML-SDARFWKSMEVTLKYVVLSVPLKLAMALFVAILLYKASLGMTIYRTLFYLPSLIGG 130 + SD RF +S++VTL +V + PLKLA ALF+A+L G ++ T++Y+PS+IGG Sbjct: 78 HIFTSDPRFVQSLKVTLIFVFVPTPLKLAFALFLAMLFNTGRKGSGLFSTIYYIPSIIGG 137 Query: 131 SVAVAVMWRNIFGMEGLLNSFLSLMGITGKEWLGQPDYALYVLILLVVWQFGSSMVIFLA 190 SVAVAV+W+ +FG G +NSFL L G +G W+G DYAL +LILLVVWQFGS M+IFLA Sbjct: 138 SVAVAVVWKQLFGSRGAVNSFLELFGFSGVNWIGSTDYALSILILLVVWQFGSPMIIFLA 197 Query: 191 GLKNVPGDLYEAAQIDGAGSVARFFKITLPMISPILLFNLILQTISSFQVFTQGYIITKG 250 GL+ +P +LYEAA +DGA S +FF ITLPM++P++ FNLI+QTI F FTQ Y+IT G Sbjct: 198 GLRQIPQELYEAASVDGANSFRKFFSITLPMLTPVIFFNLIMQTIGGFMTFTQAYLITGG 257 Query: 251 GPMDETLFSVLYIYDLAFKQQRMGYASAVSWXXXXXXXXXXXXXXXXSKRWVYYEND 307 GP+D TLF +Y+Y++AF+ RMGYASA++W S WV+YE++ Sbjct: 258 GPLDSTLFYAVYLYEVAFEYLRMGYASAMAWILLVIIGGITFVFFKSSSYWVHYESE 314 >C5C4L6.1 C5C4L6_9MICO Binding-protein-dependent transport systems inner membrane component Length = 324 Score = 286 bits (731), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 151/317 (47%), Positives = 200/317 (63%), Gaps = 14/317 (4%) Query: 6 TRPAVTVKPTKKAGKSKNV-----------LIAYLFLLPWLIGLFVLTLWPFINS-FYXX 53 TR +PT +NV L AY FLLPWLIGL VLT+ P S +Y Sbjct: 4 TRSLSATRPTAAVVVPRNVTPPLRSRLRASLPAYGFLLPWLIGLVVLTIGPMALSLYYSF 63 Query: 54 XXXXXXXXXXFVGLQNYIDMLSDARFWKSMEVTLKYVVLSVPLKLAMALFVAILLYKASL 113 ++GL NY +M SD R+ S VTL YV++SVPL++ +AL VA+LL + Sbjct: 64 SEYSLLAPPEWIGLDNYREMFSDPRYLASARVTLTYVLVSVPLEMVIALGVAMLLNRGLR 123 Query: 114 GMTIYRTLFYLPSLIGGSVAVAVMWRNIFGMEGLLNSFLSLMGITGKEWLGQPDYALYVL 173 G+ YR ++Y+PSL+GGSVA+A++WR IFG EGL+N L GI W+ PD +L+ L Sbjct: 124 GIAAYRAVYYVPSLLGGSVAIAILWREIFGSEGLVNDVLGWFGIESGSWVTNPDTSLWTL 183 Query: 174 ILLVVWQFGSSMVIFLAGLKNVPGDLYEAAQIDGAGSVARFFKITLPMISPILLFNLILQ 233 I+L VWQFG+ MVIFLAGL+ +P ++ EAAQ+DGAG+V RF +ITLP++SPI+ FNL+LQ Sbjct: 184 IILRVWQFGAPMVIFLAGLRQIPSEVLEAAQVDGAGAVRRFLRITLPLLSPIVFFNLVLQ 243 Query: 234 TISSFQVFTQGYIIT--KGGPMDETLFSVLYIYDLAFKQQRMGYASAVSWXXXXXXXXXX 291 I SFQ FT YI++ GGP D TLF LY+Y AF RMGYA+A++W Sbjct: 244 IIGSFQAFTPAYIVSGGSGGPADSTLFYTLYLYQEAFGNFRMGYAAAMAWVLLLVIAAFT 303 Query: 292 XXXXXXSKRWVYYENDT 308 S+ WV+YE+DT Sbjct: 304 AANFLMSRFWVFYESDT 320 >Q5WL27.1 Q5WL27_BACSK Lactose ABC transporter permease Length = 314 Score = 283 bits (724), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 143/308 (46%), Positives = 194/308 (62%), Gaps = 2/308 (0%) Query: 2 ETVQTRPAVTVKPTKKAGKSKNVLIAYLFLLPWLIGLFVLTLWPFINS-FYXXXXXXXXX 60 ET++ K + K K L+ + F+ PWL+G + P I S +Y Sbjct: 5 ETIRAEYPPNRKTSAKWRVWKQYLVGFAFISPWLVGFLGFVIGPMIASLYYSFTDFDMLT 64 Query: 61 XXXFVGLQNYIDMLS-DARFWKSMEVTLKYVVLSVPLKLAMALFVAILLYKASLGMTIYR 119 +VGL NYI M + D RF S+ VTL +V +S P+KLA AL +A+L G Y Sbjct: 65 SPNWVGLDNYITMFTNDPRFRTSITVTLVFVFVSTPIKLAFALLIAMLFNNKRKGSGFYT 124 Query: 120 TLFYLPSLIGGSVAVAVMWRNIFGMEGLLNSFLSLMGITGKEWLGQPDYALYVLILLVVW 179 TLFY+PS+IGGSVAVAVMWR +FG +G +N L G+ GK W+ PD+AL VLILLV+W Sbjct: 125 TLFYIPSIIGGSVAVAVMWRQLFGGQGAINDILLFFGVEGKSWIASPDHALMVLILLVIW 184 Query: 180 QFGSSMVIFLAGLKNVPGDLYEAAQIDGAGSVARFFKITLPMISPILLFNLILQTISSFQ 239 QFGS M+IFLAGL+ +P DLYEAA +DGAG +F IT+P+++P++ FNLI+Q I +F Sbjct: 185 QFGSPMIIFLAGLRQIPEDLYEAASVDGAGKFRKFLSITIPLLTPVIFFNLIMQMIGAFL 244 Query: 240 VFTQGYIITKGGPMDETLFSVLYIYDLAFKQQRMGYASAVSWXXXXXXXXXXXXXXXXSK 299 VFTQ ++ T+GGP+D TLF LY+Y+ AF MGYASA++W SK Sbjct: 245 VFTQAFVTTQGGPLDRTLFYALYLYERAFTNYEMGYASAMAWTLLIVIAIVTGLLFYSSK 304 Query: 300 RWVYYEND 307 +WV+YE++ Sbjct: 305 KWVFYESE 312 >B3KAH3.1 B3KAH3_9BACI Binding-protein-dependent transport systems inner membrane component Length = 317 Score = 279 bits (713), Expect = 3e-73, Method: Compositional matrix adjust. Identities = 148/307 (48%), Positives = 202/307 (65%), Gaps = 5/307 (1%) Query: 10 VTVKPTKKAGKSKNVLIAYLFLLPWLIGLFVLTLWPFINS-FYXXXXXXXXXXXXFVGLQ 68 V KP +K S L YLF+ PWLIG LT +P + S +Y +VGL Sbjct: 13 VRKKPFRKRWDSP--LAGYLFISPWLIGFLALTAYPMLLSLYYSFTNYTLMKPIEWVGLD 70 Query: 69 NYIDMLS-DARFWKSMEVTLKYVVLSVPLKLAMALFVAILLYKASLGMTIYRTLFYLPSL 127 NY +++ D +F +S+++T YV+ SVPLKL AL VA+LL KA G+++YRT Y PSL Sbjct: 71 NYERIITADDKFAQSLKLTFYYVLASVPLKLIAALMVALLLKKAVRGISVYRTAIYFPSL 130 Query: 128 IGGSVAVAVMWRNIFGMEGLLNSFLSLMGITGKEWLGQPDYALYVLILLVVWQFGSSMVI 187 IG S+AV+++W+NIFG++G N L GI G W+ PD AL+ LI L VWQFGSSMVI Sbjct: 131 IGASIAVSLLWKNIFGVDGFFNQVLGFFGIPGTGWVTNPDTALWSLIGLSVWQFGSSMVI 190 Query: 188 FLAGLKNVPGDLYEAAQIDGAGSVARFFKITLPMISPILLFNLILQTISSFQVFTQGYII 247 FLAGLK +P DLYEA+ +DGAG + +FFKITLPM+SP L FNLI+ I+SFQ+FT Y+I Sbjct: 191 FLAGLKQIPNDLYEASAVDGAGKLKQFFKITLPMLSPTLFFNLIMGVINSFQMFTPAYVI 250 Query: 248 TKGGPMDETLFSVLYIYDLAFKQQRMGYASAVSWXXXXXXXXXXXXXXXXSKRWVYYEND 307 T GGPM+ T LY+Y+ AF + ++GY+S+++W SK WV+YE++ Sbjct: 251 TGGGPMNSTYVYALYLYERAFSRYQLGYSSSLAWIMLILIVAAAVIINLTSKYWVFYESE 310 Query: 308 TK-ARGK 313 ++ +GK Sbjct: 311 SQGGKGK 317 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.327 0.141 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,635,924,670 Number of extensions: 61781750 Number of successful extensions: 238946 Number of sequences better than 1.0e-05: 5859 Number of HSP's gapped: 230005 Number of HSP's successfully gapped: 5871 Length of query: 313 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 178 Effective length of database: 1,788,859,178 Effective search space: 318416933684 Effective search space used: 318416933684 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 131 (55.1 bits)