BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0454.0006.p (433 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K418.1 B3K418_9BACI Extracellular solute-binding protein family 1 184 2e-44 B3KAH6.1 B3KAH6_9BACI Extracellular solute-binding protein family 1 172 5e-41 B9JLC9.1 B9JLC9_AGRRK Sugar ABC transporter 165 7e-39 A4AJA3.1 A4AJA3_9ACTN Sugar-binding protein 161 1e-37 A6UFG3.1 A6UFG3_SINMW Extracellular solute-binding protein family 1 154 2e-35 >B3K418.1 B3K418_9BACI Extracellular solute-binding protein family 1 Length = 438 Score = 184 bits (466), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 120/343 (34%), Positives = 182/343 (53%), Gaps = 19/343 (5%) Query: 27 DAGTANSAAD---TVELRFAWWGSEDRHTATLEAIKIFEEKHPGIKILPEYSGFDGYESK 83 + G A++ A+ VELR WWG + R T EA+K+F+ KHP IKI+ E+S GY K Sbjct: 28 EGGNADAGAEPSGQVELRMMWWGDQKRADITNEALKVFQSKHPNIKIVGEFSPSSGYFDK 87 Query: 84 IQAQIAGNSAPDLFQSTGEINVRLGADSLLPLD----GVLDTTGMSDSIMESVAMDGKTV 139 + Q+A +APD+F G + D LL LD L GM +++E +DGK Sbjct: 88 LNTQLASGTAPDIFFLGGNVVDYAKKDVLLNLDPYVGSELKLDGMDATMVEYGRLDGKLQ 147 Query: 140 GAYIGLAGDAYLYDETLLKQLGVEPPVPPYSWDDLAAKFKEIAEKSGGKVYGAVDSSV-A 198 G + ++ L ++ G+ P + W+D AA KE+++K G VYG + +V Sbjct: 148 HISAGANARGIVINKALFEKAGIPLPEQDWDWEDFAAISKELSDKLGDGVYGTYNFTVDG 207 Query: 199 FDIF-KNYGITALDASAPFPNSDSAFTFTEEQIRSYYQYWDDLRKAGAVAPPDLSATS-- 255 DIF K G D + F +E I+ +++YW+ KAG V P+L ++ Sbjct: 208 MDIFLKQRGKQLYDM------ENGKLGFAKEDIQEWFEYWEQASKAGGVVTPELQVSNPH 261 Query: 256 DDSANSLLVKGKAAFVPIASSSFSRLQSQTKNTLNMIMLPQSAKTGVTVTPGTSQVLVAS 315 DD++ SLL+ GKAA + S+ + QS T++ L + +P+ K G V +SQ L Sbjct: 262 DDTSKSLLITGKAAMSLLPSNQLAAFQSLTEDPLILHPVPRGPK-GTGVVFESSQGLSGY 320 Query: 316 AKTKHPQEVAEFINFFVHDTDAANALKTTRGVLPTTQQQEALL 358 A TKHP+EVA ++F+++D +AA L RGV P T+ LL Sbjct: 321 ANTKHPKEVATLMDFWINDPEAAKILGNDRGV-PVTEANRNLL 362 >B3KAH6.1 B3KAH6_9BACI Extracellular solute-binding protein family 1 Length = 441 Score = 172 bits (437), Expect = 5e-41, Method: Compositional matrix adjust. Identities = 117/401 (29%), Positives = 194/401 (48%), Gaps = 19/401 (4%) Query: 31 ANSAADTVELRFAWWGSEDRHTATLEAIKIFEEKHPGIKILPEYSGFDGYESKIQAQIAG 90 + SAA+ V+LR WWGS+ RH ATL+A++ + +K+P + PEYSG DGY K+ Q A Sbjct: 47 SGSAAENVKLRIMWWGSQPRHEATLKALETYTQKNPHVTFEPEYSGMDGYLDKLSTQAAA 106 Query: 91 NSAPDLFQ-STGEINVRLGADSLLPLDGVLDTTGMSDSIMESVAMDGKTVGAYIGLAGDA 149 ++ PD+FQ G ++ + L LD + ++ S++ DGK G +G Sbjct: 107 HNEPDIFQMDPGWVSDWTSRNQLEELDTKVRLDELTPSLLPIGQRDGKQYGVPLGSVAFG 166 Query: 150 YLYDETLLKQLGVEPPVPPYSWDDLAAKFKEIAEKSGGKVYGAVDSSVAFDIFKNYGITA 209 +YD+ L++LG P ++WDD A +E+ K Y +D + + +F Y Sbjct: 167 MIYDKAALEKLGATMPTDGWTWDDFFALAEEVKPKLAEGQYFTLDYAGNYFMFSAYHYAK 226 Query: 210 LDASAPFPNSDSAFTFTEEQIRSYYQYWDDLRKAGAVAPPDLSATSD--DSANSLLVKGK 267 D F E + + ++ LRK G + P DL+A+ D LLV GK Sbjct: 227 --GKGTLITEDGKFNIDEATFLEWTKKFEQLRKDGLIPPADLNASDKEMDPTADLLVNGK 284 Query: 268 AAFVPIASSSFSRLQSQTKNTLNMIMLPQSAKTGVTVTPGTSQVLVASAKTKHPQEVAEF 327 F S++++ S + +++ +P++ + G + P S S +KH +E A+F Sbjct: 285 ILFRYSFSNNYTTWDSMKEGAYDLVTMPRAEEAGGWLKP--SMYFSLSPNSKHKEEAAKF 342 Query: 328 INFFVHDTDAANALKTTRGVLPTTQQQEALLANADLSEGDKKNIDLIQQLNKLEXXXXXX 387 I++F++D +A L T RGV + E+L+ N L EG+K + LI + Sbjct: 343 IDWFINDPEAGAILGTARGVPANAKIAESLIPN--LPEGEKVGMKLI------DATTPDG 394 Query: 388 XXXXXQVLGWKGLIEK----VGQEIAFGKISVDDGAKKLMK 424 GW I+K V E++FG+ + + + L K Sbjct: 395 QIFTTGPEGWVNFIDKDFPLVRDELSFGRTTPEKAYESLKK 435 >B9JLC9.1 B9JLC9_AGRRK Sugar ABC transporter Length = 425 Score = 165 bits (418), Expect = 7e-39, Method: Compositional matrix adjust. Identities = 123/409 (30%), Positives = 198/409 (48%), Gaps = 35/409 (8%) Query: 35 ADTVELRFAWWGSEDRHTATLEAIKIFEEKHPGIKILPEYSGFDGYESKIQAQIAGNSAP 94 A+T LR AWWG +R T E +++++++ G+++ E S + Y +K+ Q+ G SAP Sbjct: 29 AETTRLRLAWWGGNERTRRTQEVLRLYQQRVNGVELAGEVSNSE-YFTKLATQVVGRSAP 87 Query: 95 DLFQ----STGEINVRLGADSLLPLDGVL----DTTGMSDSIMESVAMDGKTVGAYIGLA 146 D+FQ S + R D +L LDG++ D G+S ++++ A+DGK G L Sbjct: 88 DVFQLEPNSFADFVKR---DCVLALDGLMPNPIDAAGISRAMLDLCAVDGKVWGIPQALN 144 Query: 147 GDAYLYDETLLKQLGVEPPVPPYSWDDLAAKFKEIAEKSGGKVY-GAVDS---SVAFDIF 202 A +YD+ GV PP SWDD A + E+ + + Y G+ D+ S AF ++ Sbjct: 145 SFAMMYDKDAFATAGVAPPDAQTSWDDYADRMVELTKALNRRRYWGSCDASGYSYAFQVW 204 Query: 203 -KNYGITALDASAPFPNSDSAFTFTEEQIRSYYQYWDDLRKAGAVAPPDLSATSDDSANS 261 + G A D A F ++ + ++ YWD LR+ G P D++A S DS + Sbjct: 205 LRQRGRDLFTA-------DQALGFGKDDVAEWFAYWDKLRQRGGCVPADVAALSPDSTIN 257 Query: 262 L--LVKGKAAFVPIASSSFSRLQSQTKNTLNMIMLPQ---SAKTGVTVTPGTSQVLVASA 316 L L++GKAA S+ +Q+ T L M M P AK G P +S + A Sbjct: 258 LMPLIQGKAATSFAFSNQIVGMQATTPVQLAMTMFPNGGAGAKPGQFYRPASSWSIY--A 315 Query: 317 KTKHPQEVAEFINFFVHDTDAANALKTTRGVLPTTQQQEALLANADLSEGDKKNIDLIQQ 376 ++ +P+ A F+NFFV D +AA L RGV + + ++L L ++ ++ I Sbjct: 316 QSPNPEAAARFVNFFVSDPEAAKILGVERGVPMSPALRASILPT--LRPVERATVEYIDA 373 Query: 377 LNKLEXXXXXXXXXXXQVLGWKGLIEKVGQEIAFGKISVDDGAKKLMKE 425 + Q G L+++ +AF S+ D A LMK+ Sbjct: 374 IADKVGPYPPPPPKGSQQFG--QLMKRAADAVAFNSASISDSAATLMKD 420 >A4AJA3.1 A4AJA3_9ACTN Sugar-binding protein Length = 469 Score = 161 bits (407), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 97/331 (29%), Positives = 169/331 (51%), Gaps = 13/331 (3%) Query: 38 VELRFAWWGSEDRHTATLEAIKIFEEKHPGIKILPEYSGFDGYESKIQAQIAGNSAPDLF 97 VELRF+WWG++ RH T EA+ +FEEK+PGI ++ ++ GFDGY K+ Q AGN++PD+F Sbjct: 81 VELRFSWWGTDSRHELTNEALDLFEEKNPGITVVRDFGGFDGYIDKLLTQAAGNNSPDVF 140 Query: 98 QSTGEINVRLGADSLL-----PLDGVLDTTGMSDSIMESVAMDGKTVGAYIGLAGDAYLY 152 Q E+ + L L G ++++ +DG GL A+++ Sbjct: 141 QLYEEVLREFASRGQLYDLNEATSQGLSLDGWDQGLLDTSTIDGSLSALQFGLTTQAFIF 200 Query: 153 DETLLKQLGVEPPVPPYSWDDLAAKFKEIAEKSGGKVYGAVDSSVAFDIFKNYGITALDA 212 + L Q GV P +SWDDLA K++++ + +G D S + +F+ + + Sbjct: 201 NTELFDQAGVSIPTEGWSWDDLATAAKKVSDGTDSGTFGVTDLSTGYQVFEVWA----NQ 256 Query: 213 SAPFPNSDSAFTFTEEQIRSYYQYWDDLRKAGAVAPPDLSATSDDSANSLLVKGKAAFVP 272 + +D F+ + ++ YW DLR +GA P L++ ++++ KAA Sbjct: 257 NGESYLTDDGLGFSAGTLEDFWNYWADLRASGAATPGSLTSEYPTPFDAIIAS-KAASGF 315 Query: 273 IASSSFSRLQSQTKNTLNMIMLP-QSAKTGVTVTPGTSQVLVASAKTKHPQEVAEFINFF 331 I ++ + +QS + + + +P +S + G + T+ + +KT+HP+E A ++F Sbjct: 316 IFANQMAAVQSSIEAEIAVDRMPGESPEAGSYLR--TAMNIAIGSKTEHPKEAAMLVDFL 373 Query: 332 VHDTDAANALKTTRGVLPTTQQQEALLANAD 362 ++D +A L RGV +A AN D Sbjct: 374 LNDPEAYAILGIDRGVPANPAVGDAATANVD 404 >A6UFG3.1 A6UFG3_SINMW Extracellular solute-binding protein family 1 Length = 428 Score = 154 bits (388), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 122/412 (29%), Positives = 198/412 (48%), Gaps = 39/412 (9%) Query: 34 AADTVELRFAWWGSEDRHTATLEAIKIFEEKHPGIKILPEYSGFDGYESKIQAQIAGNSA 93 AA++V+LR WWGS DR TL K+F E +P I+I+ E DGY +K+ Q+AG S Sbjct: 28 AAESVQLRAMWWGSNDRSKRTLAVAKLFAEANPDIRIIGESLSGDGYWTKLATQMAGRSI 87 Query: 94 PDLFQ----STGEINVRLGADSLLP-LDGVLDTTGMSDSIMESVAMDGKTVGAYIGLAGD 148 D+FQ + + + R +L P + LD +++ +DGK G +GL Sbjct: 88 ADIFQLEPSTISDYSKRGACMALDPFISSALDVDAFGKDVLKLTTVDGKLWGVGLGLNSF 147 Query: 149 AYLYDETLLKQLGVEPPVPPYSWDDLAAKFKEIA---------EKSGGKVYGAVDSSVAF 199 A YD + G++PP +W A++ EIA + +GG YGA + V F Sbjct: 148 ALFYDADAFARAGIDPPGIDTTW----AEYAEIAVEMTKAVGKKSAGGGPYGARYAYV-F 202 Query: 200 DIFKNYGITALDASAPFPNSDSAFTFTEEQIRSYYQYWDDLRKAGAVAPPDLSATSDDSA 259 D + ++L +DS F E+ + +Y YW++LRK G D+ ++ Sbjct: 203 DAWLRQRGSSL-------YTDSGLGFGVEEAKEWYAYWEELRKRGGTVGADIQTLDQNTI 255 Query: 260 NS-LLVKGKAAFVPIASSSFSRLQSQTKNTLNMIMLPQSAK---TGVTVTPGTSQVLVAS 315 ++ L G +A S+ Q K+ L + MLP++ K +G P + + Sbjct: 256 DTNCLALGYSAMGMAYSNQMVGYQLIMKSKLGIGMLPRAEKGGPSGHYYRP--ALIWSIG 313 Query: 316 AKTKHPQEVAEFINFFVHDTDAANALKTTRGVLPTTQQQEALLANADLSEGDK-KNID-L 373 A T+H +E A+FINFFV+D +A L RGV + +EA+L + + +E + K I+ L Sbjct: 314 ASTEHGEEAAKFINFFVNDVEAGKILGVERGVPMSPTVREAILPSLNPTETETVKYINGL 373 Query: 374 IQQLNKLEXXXXXXXXXXXQVLGWKGLIEKVGQEIAFGKISVDDGAKKLMKE 425 Q+ Q ++ + E+AF +IS+ D A +L++E Sbjct: 374 KDQVGSYPSPAPLGATEFDQR-----VLRPIADELAFERISIGDAATRLVEE 420 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.313 0.130 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,325,273,520 Number of extensions: 90787783 Number of successful extensions: 201719 Number of sequences better than 1.0e-05: 398 Number of HSP's gapped: 201499 Number of HSP's successfully gapped: 398 Length of query: 433 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 294 Effective length of database: 1,751,175,118 Effective search space: 514845484692 Effective search space used: 514845484692 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)