BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0450.0028.p (392 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C5D1Y0.1 C5D1Y0_VARPD Alpha/beta hydrolase fold protein 219 4e-55 Q7NL16.1 Q7NL16_GLOVI Gll1310 protein 172 4e-41 B4WUK0.1 B4WUK0_9SYNE Hydrolase, alpha/beta fold family, putative 163 2e-38 Q022C4.1 Q022C4_SOLUE Alpha/beta hydrolase fold 158 8e-37 Q01VS1.1 Q01VS1_SOLUE Alpha/beta hydrolase fold 155 7e-36 >C5D1Y0.1 C5D1Y0_VARPD Alpha/beta hydrolase fold protein Length = 339 Score = 219 bits (558), Expect = 4e-55, Method: Compositional matrix adjust. Identities = 130/312 (41%), Positives = 172/312 (55%), Gaps = 16/312 (5%) Query: 77 YEQAARSRAIKDFPAPGRLVDIG-GRQIHLNCQGRGTPLVLLEAGADTSGSALWQPVQEQ 135 YEQ R RA++DFPAPG++VD+G GR+I L+C+G G+P V+ EAG GS W VQ + Sbjct: 30 YEQIGRYRAVRDFPAPGKMVDVGAGRRIQLDCRGTGSPTVVFEAGLSVDGSLSWSAVQPK 89 Query: 136 VARFTRVCSYDRAGIMWSDPAKGQRNSHAIAEDLHKVLRAANEQPPYILVGASMGGPYVM 195 VA TR C+Y RAG+MWSDP+ + DLH VL + E+ P++L G S+GG Y + Sbjct: 90 VAAHTRACAYSRAGLMWSDPSNTPPGAAQAVHDLHAVLAKSGERGPFVLAGQSLGGLYAL 149 Query: 196 TFTKYYRKEVAGMVLVDASHPDQTARLAQATGEPAS--NPIPIA---FRALAAVAWTGLP 250 FT + EVAG+VLVD SHPDQ R+A E S + I IA F L A A LP Sbjct: 150 GFTHRFGAEVAGLVLVDPSHPDQVERVAHFMTESRSMRSRIGIALAPFGVLRAAAPVLLP 209 Query: 251 RLVLPAAEVPELPSQTAKAITAYQPTSLXXXXXXXXXXXDSLREAGTFRTLGHLPLAVLS 310 E P +Q +A+ A+ P SL SL EA F+ LG PL VL+ Sbjct: 210 -------ESPHHSAQDMQAVRAFAPKSLRTQLAESDAVDASLAEAMDFQHLGDRPLVVLT 262 Query: 311 RGKPWSAFSEAERAAGGLTREQFNRAEAAWASMQAEEAGWSSNSTHQVLTDSSHVMQLER 370 P++A AE LT EQ + +A W M E A WSS ++ + H +Q + Sbjct: 263 AMAPYTA---AELRGMKLTPEQGRQVQAIWQHMHGEMAAWSSRGRQLLVAGAGHDIQFDE 319 Query: 371 PDAVIAAIKDVV 382 P AV+ A+ VV Sbjct: 320 PQAVVDAVLSVV 331 >Q7NL16.1 Q7NL16_GLOVI Gll1310 protein Length = 341 Score = 172 bits (437), Expect = 4e-41, Method: Compositional matrix adjust. Identities = 125/339 (36%), Positives = 169/339 (49%), Gaps = 35/339 (10%) Query: 64 SILAIALVSTGGIYEQAARSRAIKDFPAPGRLVDIGGRQIHLNCQGRGTPLVLLEAGADT 123 ++ + G IY+ A + +P PGRLVD+GG ++HL G G P V+LEAG Sbjct: 15 ALFIFVAIDVGAIYQAVASIADRRKWPPPGRLVDVGGHRLHLLEVGTGGPTVILEAGGG- 73 Query: 124 SGSALWQPVQEQVARFTRVCSYDRAGIMWSDPAKGQRNSHAIAEDLHKVLRAANEQPPYI 183 + S W VQ +VA+FTR SYDRAG WSD S ++E+LH +L A PYI Sbjct: 74 AWSLEWCRVQAEVAKFTRTVSYDRAGYGWSDAGLKNPTSRQVSEELHALLEKAEIAGPYI 133 Query: 184 LVGASMGGPYVMTFTKYYRKEVAGMVLVDASHPDQTARL---AQATGEPASNPI------ 234 LVG S+GG ++ F Y +EVAG+VLVDASH D+ R+ +A GE N + Sbjct: 134 LVGRSLGGLHMRMFAHLYPQEVAGVVLVDASHEDENRRILPQLRAAGERNLNRLGWLRLL 193 Query: 235 -PIAFRALAAVAWTGLPRLVLPAAEVPELPSQTAKAITA-----YQPTSLXXXXXXXXXX 288 PI A W LP+L + +LP+Q A A ++L Sbjct: 194 APIGLIRFAG-WWEILPKL----GTIEKLPAQRQDAARAGIFRSLYCSTLYSEYRHFDES 248 Query: 289 XDSLREAGTFRTLGHLPLAVLSRGKPWSAFSEAERAAGGLTREQF--NRAEAAWASMQAE 346 D +R AG+ R LPLAVL + A R+ F + W +QAE Sbjct: 249 ADQVRAAGSLRD---LPLAVL---------TAANHLDPADYRQDFPIEASRRIWLELQAE 296 Query: 347 EAGWSSNSTHQVLTDSSHVMQLERPDAVIAAIKDVVGKA 385 A SS+S VL D + + LERP+AV+AAI+ V KA Sbjct: 297 LAALSSDSHQSVLPDCGNYVSLERPEAVVAAIRSVYLKA 335 >B4WUK0.1 B4WUK0_9SYNE Hydrolase, alpha/beta fold family, putative Length = 322 Score = 163 bits (413), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 109/337 (32%), Positives = 173/337 (51%), Gaps = 36/337 (10%) Query: 65 ILAIALVSTGGIYEQAARSRAIKDFPAPGRLVDIGGRQIHLNCQGRGTPLVLLEAGADTS 124 I+AI+L+ TG +Y+ + + + +P G L+DIGG ++HLNC G+GTP V+++AG + Sbjct: 6 IVAISLL-TGILYQVVSTAIDQRRYPPQGELIDIGGFRLHLNCMGQGTPTVVMDAGG-CA 63 Query: 125 GSALWQPVQEQVARFTRVCSYDRAGIMWSDPA-KGQRNSHAIAEDLHKVLRAANEQPPYI 183 S W V ++A+F RVC YDRAG WS+P R S A++LH +L A +PPYI Sbjct: 64 PSIAWGLVPSEIAKFARVCIYDRAGFGWSEPNFSAARTSQQSADELHLLLSKAEIEPPYI 123 Query: 184 LVGASMGGPYVMTFTKYYRKEVAGMVLVDASHPDQTARLAQATGEPASNPIPIAFRALAA 243 LVG S+GG + + + +++AG+VLVD+SH +Q + E ++ Sbjct: 124 LVGHSLGGVNMRLYASQHLEDIAGLVLVDSSHENQMT----SEMERRIKMTSWLYQGFRI 179 Query: 244 VAWTGLPRLVLPAAEVP---ELPSQTAKAITA------------YQPTSLXXXXXXXXXX 288 V+ GL RL+ +P E+ +T A Y+P Sbjct: 180 VSQFGLLRLIGEMNLLPVLKEIKQETQNYPLAVQSLFDTFKSFCYRPDYWAAASSELANI 239 Query: 289 XDSLREAGTFRTLGHLPLAVLSRGKPWSAFSEAERAAGGLTREQFNRAEAAWASMQAEEA 348 +SL + +LG LPL VLS+G S S AER WA++Q + Sbjct: 240 KESLEGIRSVSSLGSLPLIVLSQGSKDSTVS-AERV-------------ERWAALQLDLT 285 Query: 349 GWSSNSTHQVLTDSSHVMQLERPDAVIAAIKDVVGKA 385 SS S H + DS H++ L++P+ +++A++ + +A Sbjct: 286 NLSSCSQHVIAEDSGHLIPLDQPELIVSAVRQLTDRA 322 >Q022C4.1 Q022C4_SOLUE Alpha/beta hydrolase fold Length = 334 Score = 158 bits (400), Expect = 8e-37, Method: Compositional matrix adjust. Identities = 107/313 (34%), Positives = 157/313 (50%), Gaps = 9/313 (2%) Query: 74 GGIYEQAARSRAIKDFPAPGRLVDIGGRQIHLNCQGRGTPLVLLEAGADTSGSALWQPVQ 133 G +Y++ A + P PGRLVD+GG +++L+C G G+P V+L++ S W P+Q Sbjct: 25 GFVYQRVAERYERGNLPRPGRLVDVGGHRLYLSCTGEGSPTVVLDSALGVV-SYSWTPIQ 83 Query: 134 EQVARFTRVCSYDRAGIMWSDPAKGQRNSHAIAEDLHKVLRAANEQPPYILVGASMGGPY 193 +V+RFTRVCSYDRAG SDP R S +A +LH +L A E PY+LVG S+GG Sbjct: 84 AEVSRFTRVCSYDRAGYGLSDPGPLPRTSKQLASELHSLLGTAEEAGPYVLVGHSLGGLT 143 Query: 194 VMTFTKYYRKEVAGMVLVDASHPDQTARLAQATGEPASNPIPIAFRALAAVAWTGLPRLV 253 + FT+ + EVAG+VLVD++ + A L P P + +A G+ R+V Sbjct: 144 IRLFTQAHPAEVAGLVLVDSASENAEAHLTPEL-RPLMMPPLTQMKFAGVLAEFGMHRIV 202 Query: 254 LPAAE-VPELPSQTAKAITAYQPTSLXXXXXXXXXXXDSLREAGTFRTLGHLPLAVLSRG 312 L + +Q A A+ + + S + + LG PL VL+RG Sbjct: 203 LSMRKGRGSTEAQRAAALNLRRRAYIQAAIGELEALPLSTEQVRSAGKLGDRPLIVLTRG 262 Query: 313 KPWSAFSEAERAAGGLTREQFNRAEAAWAS-MQAEEAGWSSNSTHQVLTDSSHVMQLERP 371 EA R E F R W + +QA G S+ + S H++ E P Sbjct: 263 PVHPG--EAPRGVSPAIYENFER---IWRTEIQATMVGLSTRGEQRFAKHSGHMIPQEEP 317 Query: 372 DAVIAAIKDVVGK 384 +AVI I++VV + Sbjct: 318 EAVIDGIREVVSR 330 >Q01VS1.1 Q01VS1_SOLUE Alpha/beta hydrolase fold Length = 350 Score = 155 bits (392), Expect = 7e-36, Method: Compositional matrix adjust. Identities = 119/337 (35%), Positives = 159/337 (47%), Gaps = 41/337 (12%) Query: 68 IALVSTGGIYEQAARSRAIKDFPAPGRLVDIGGRQIHLNCQGRGTPLVLLEAGADTSGSA 127 L S G IYE A + FP GR + IG +++L+C GRG V+L++G G A Sbjct: 27 FVLASAGAIYEAAGNWIDARRFPQQGRSIQIGSLRLNLDCSGRGGITVILDSG--MGGPA 84 Query: 128 L-WQPVQEQVARFTRVCSYDRAGIMWSDPAKGQRNSHAIAEDLHKVLRAANEQPPYILVG 186 L W VQ +VA+FTRVCSYDRAG WSD + R S IA +L +L AA E+ P+++VG Sbjct: 85 LDWILVQPEVAKFTRVCSYDRAGYGWSDASPEPRTSRQIAGELKSLLDAAGEKGPFVMVG 144 Query: 187 ASMGGPYVMTFTKYYRKEVAGMVLVDASHPD------------QTARLAQ-----ATGEP 229 S GG V F Y ++VAGMVLVDASHPD Q AR Q T E Sbjct: 145 HSFGGYNVRVFAGRYPRDVAGMVLVDASHPDEGSRTDAVLSGAQLARQQQDERRDETWEQ 204 Query: 230 ASNPIPIAFRALAAVAWTGLPRLVLPAAEVP--ELPSQTAKAITAYQ--PTSLXXXXXXX 285 + P+ I G+ R + P L + + + P + Sbjct: 205 ITEPLTIHL---------GIRRATVALGRKPSRHLSRRLQEEFLYLENMPKFMRTVRQED 255 Query: 286 XXXXDSLREAGTFRTLGHLPLAVLSRGKPWSAFSEAERAAGGLTREQFNRAEAAWA-SMQ 344 S +A LG PL VL+ GKP+ LT +Q R W +Q Sbjct: 256 AMFKISGVQAVAAGPLGERPLIVLTAGKPYEPDPL-------LTPQQIERQANIWIRELQ 308 Query: 345 AEEAGWSSNSTHQVLTDSSHVMQLERPDAVIAAIKDV 381 EE S+ V+ DS+H M +RPDA+++AI V Sbjct: 309 VEEMRLSTRGEQIVVPDSTHDMPQDRPDAIVSAIHGV 345 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.130 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,254,349,179 Number of extensions: 89196996 Number of successful extensions: 244691 Number of sequences better than 1.0e-05: 278 Number of HSP's gapped: 244852 Number of HSP's successfully gapped: 278 Length of query: 392 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 254 Effective length of database: 1,760,596,133 Effective search space: 447191417782 Effective search space used: 447191417782 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)