BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0450.0016.p (328 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIP3.1 B3KIP3_9BACI FAD-dependent pyridine nucleotide-disulphi... 458 e-127 B1DLX8.1 B1DLX8_9BACL FAD-dependent pyridine nucleotide-disulphi... 358 5e-97 C0ZCS1.1 C0ZCS1_BREBN Putative uncharacterized protein ypdA 342 2e-92 A8VXS0.1 A8VXS0_9BACI Formate-tetrahydrofolate ligase, FTHFS 337 1e-90 C3IYR1.1 C3IYR1_9BACI FAD-dependent pyridine nucleotide-disulphi... 325 3e-87 >B3KIP3.1 B3KIP3_9BACI FAD-dependent pyridine nucleotide-disulphide oxidoreductase Length = 325 Score = 458 bits (1179), Expect = e-127, Method: Compositional matrix adjust. Identities = 215/324 (66%), Positives = 259/324 (79%) Query: 1 MKDVIIIGAGPCGLSAAIECQRRGLSSLIIEKNFIVHSIYLYPTNMQFFSTTALLEIGDV 60 M DV+IIGAGPCGL+AAIEC R+GLS II+K+ +VHSIYLYPT+MQFFST +LEIG+V Sbjct: 1 MLDVLIIGAGPCGLAAAIECGRQGLSCEIIDKHNVVHSIYLYPTHMQFFSTAEMLEIGEV 60 Query: 61 PFTSPNDKPFRHEALVYYRRAAEQHGLDIAAYEEALSVKPLEDGTFAVHTRNKRGETYTR 120 PF NDKP+RHEAL YYRR A+ L+I+ YEEAL++ DG+F V TR + GE R Sbjct: 61 PFAISNDKPYRHEALAYYRRVAKHFNLNISPYEEALTIAKQPDGSFQVKTRRRGGEETLR 120 Query: 121 TAANVIISTGYFDQPNLIGIPGEELPKVTHYFGEAHPYTGMKVTVIGGSNSAVDAALELL 180 A +V+I+TGYFD PN IGIPGE+L KVTHYF EAHPY GMK +IGGSNSAVDAA+EL+ Sbjct: 121 QAKHVVIATGYFDHPNYIGIPGEDLEKVTHYFREAHPYAGMKTAIIGGSNSAVDAAMELI 180 Query: 181 RVGAEVDMVYRGASISENIKPWVRPIFESMVAKDKIVLHLESRVTEITPGSVHITGRNGE 240 RVGAEV MVYRG +S+NIKPWVRP+F+SMV K I LHL SRV +I S+ + G+ Sbjct: 181 RVGAEVTMVYRGEEVSQNIKPWVRPLFDSMVQKGHIQLHLNSRVVQIAESSITVLSNQGQ 240 Query: 241 VKELDNDFVLALTGFRPSRTLLSSAGVSMDETQEKPAFNPSTMESNVPGLYIAGVIASGR 300 ++NDFVLALTGFRP R LL+S+GV +DE EKP +NP TME+NVPGLY+AGVIASGR Sbjct: 241 ESIIENDFVLALTGFRPDRKLLASSGVQLDEDMEKPLYNPDTMETNVPGLYVAGVIASGR 300 Query: 301 NANEVFIETGRGHGKLIADHIISK 324 NANEVFIETGR HG+LIA+H+ +K Sbjct: 301 NANEVFIETGRLHGRLIAEHLGTK 324 >B1DLX8.1 B1DLX8_9BACL FAD-dependent pyridine nucleotide-disulphide oxidoreductase Length = 325 Score = 358 bits (918), Expect = 5e-97, Method: Compositional matrix adjust. Identities = 182/324 (56%), Positives = 235/324 (72%), Gaps = 2/324 (0%) Query: 2 KDVIIIGAGPCGLSAAIECQRRGLSSLIIEKNFIVHSIYLYPTNMQFFSTTALLEIGDVP 61 ++ IIIGAGPCGL+AAIE +R GL +IIEK+ I HSI +YPT M FFST LEIG++P Sbjct: 4 EEAIIIGAGPCGLAAAIELERIGLHPVIIEKSNIAHSISMYPTYMHFFSTPERLEIGEIP 63 Query: 62 FTSPNDKPFRHEALVYYRRAAEQHGLDIAAYEEALSVKPLEDGTFAVHTRNKRGETYTRT 121 FT+PNDKP R EA+ YYR AA ++ L + AYE L ++ ++G F + + ++ GE Sbjct: 64 FTTPNDKPSRLEAINYYRTAALRNSLRVNAYETVLRIERQDNGLFHLTSADRYGEEKQYE 123 Query: 122 AANVIISTGYFDQPNLIGIPGEELPKVTHYFGEAHPYTGMKVTVIGGSNSAVDAALELLR 181 A V+++TGYFD PN +GIPGEE+ KVTH+F EAHPYTGM+V +IGGSNSA+DAALEL R Sbjct: 124 ARYVVVATGYFDHPNYLGIPGEEMDKVTHFFREAHPYTGMEVAIIGGSNSAIDAALELER 183 Query: 182 VGAEVDMVYRGASISENIKPWVRPIFESMVAKDKIVLHLESRVTEITPGSVHITGRNGEV 241 VGA+V ++YRGA S +IKPWVRP FE+ V I + ESRV EI V + G Sbjct: 184 VGAKVTVIYRGAGYSSSIKPWVRPGFEAKVNNGIIRMLFESRVVEIDKRHVIVESPEGR- 242 Query: 242 KELDNDFVLALTGFRPSRTLLSSAGVSMDETQEKPAFNPSTMESNVPGLYIAGVIASGRN 301 + NDFVLALTGF P R L+S+GV ++E + P F+ +TME+NVPGLY+AGV+AS Sbjct: 243 HPVANDFVLALTGFHPDRDFLTSSGVRIEE-EGFPYFDETTMETNVPGLYLAGVVASKHE 301 Query: 302 ANEVFIETGRGHGKLIADHIISKR 325 ANE+FIE+GR HG IA+HI+S R Sbjct: 302 ANEIFIESGRFHGNKIAEHIVSLR 325 >C0ZCS1.1 C0ZCS1_BREBN Putative uncharacterized protein ypdA Length = 322 Score = 342 bits (878), Expect = 2e-92, Method: Compositional matrix adjust. Identities = 174/324 (53%), Positives = 224/324 (69%), Gaps = 4/324 (1%) Query: 1 MKDVIIIGAGPCGLSAAIECQRRGLSSLIIEKNFIVHSIYLYPTNMQFFSTTALLEIGDV 60 M+DV+I+G GPCGL+AAI C+R GL +IIEK +VHSIY YPT M F ST LLEIG V Sbjct: 1 MEDVLIVGGGPCGLAAAIACKRAGLDPIIIEKGSLVHSIYRYPTYMIFHSTPDLLEIGGV 60 Query: 61 PFTSPNDKPFRHEALVYYRRAAEQHGLDIAAYEEALSVKPLEDGTFAVHTRNKRGETYTR 120 PF + NDKP R EAL YYR A + L + YE K +G F + T ++ +T+T Sbjct: 61 PFATANDKPTRQEALNYYRLVAAREKLRVNVYETVTEAKKTPEG-FQITTTDRFTKTHTY 119 Query: 121 TAANVIISTGYFDQPNLIGIPGEELPKVTHYFGEAHPYTGMKVTVIGGSNSAVDAALELL 180 A N++I+TGYFD PN + +PGEELPKV+ ++ EAHPYTGMKV V+GG+NSAVDAA+EL Sbjct: 120 EAKNLVIATGYFDNPNRLQVPGEELPKVSSFYKEAHPYTGMKVAVVGGNNSAVDAAMELE 179 Query: 181 RVGAEVDMVYRGASISENIKPWVRPIFESMVAKDKIVLHLESRVTEITPGSVHITGRNG- 239 R GAEV ++ R +S+ +K W RP+FES++ K +I + + V I S+ + R G Sbjct: 180 RAGAEVTVICRRTELSDKVKAWTRPVFESLIKKGRIHMLFGATVESIEERSIRV--RMGD 237 Query: 240 EVKELDNDFVLALTGFRPSRTLLSSAGVSMDETQEKPAFNPSTMESNVPGLYIAGVIASG 299 EV L ND V +L G+RP RT L S GV +DE PA +P TME+N+PGLYIAGVIA+G Sbjct: 238 EVITLANDHVFSLIGYRPDRTFLHSLGVMIDEETGIPAHHPETMETNIPGLYIAGVIAAG 297 Query: 300 RNANEVFIETGRGHGKLIADHIIS 323 R+AN +FIE GR HG+ IA HI S Sbjct: 298 RHANAIFIENGRFHGEGIARHIAS 321 >A8VXS0.1 A8VXS0_9BACI Formate-tetrahydrofolate ligase, FTHFS Length = 329 Score = 337 bits (863), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 163/320 (50%), Positives = 215/320 (67%) Query: 2 KDVIIIGAGPCGLSAAIECQRRGLSSLIIEKNFIVHSIYLYPTNMQFFSTTALLEIGDVP 61 + VIIIGAGPCGLSAAIEC++ G +IE+ IVH++Y YPT+ QFFST+ L IGD+P Sbjct: 4 RKVIIIGAGPCGLSAAIECEKAGFDVTVIERGNIVHALYRYPTHQQFFSTSEKLAIGDIP 63 Query: 62 FTSPNDKPFRHEALVYYRRAAEQHGLDIAAYEEALSVKPLEDGTFAVHTRNKRGETYTRT 121 F S KP R+EALVYYR+ E++GL I AYEE + L F V + + G T R Sbjct: 64 FYSTERKPKRNEALVYYRKVVEEYGLTIEAYEEVTKIDRLGIHDFKVTSVKQNGLTMERD 123 Query: 122 AANVIISTGYFDQPNLIGIPGEELPKVTHYFGEAHPYTGMKVTVIGGSNSAVDAALELLR 181 A VI++TGY+D PN + IPGE+ V HYF EAHP+ KV VIGG NSA+DAALEL + Sbjct: 124 AHAVIVATGYYDSPNYMHIPGEDQKHVFHYFHEAHPFYNQKVAVIGGKNSAIDAALELEK 183 Query: 182 VGAEVDMVYRGASISENIKPWVRPIFESMVAKDKIVLHLESRVTEITPGSVHITGRNGEV 241 GAEV + R + S +IKPW+ P FES+V + KI++H E+ + EI ++ ++ G Sbjct: 184 AGAEVSVYCRYDNYSSSIKPWILPDFESLVKQGKIMMHFETEIHEIGEDTISVSDVTGTK 243 Query: 242 KELDNDFVLALTGFRPSRTLLSSAGVSMDETQEKPAFNPSTMESNVPGLYIAGVIASGRN 301 + DFV A+TG+ P + + S GV +D +P FN +ME+NV G+YIAGVIA+G N Sbjct: 244 RNDRADFVFAMTGYHPDHSFIRSMGVEVDMETGRPVFNSESMETNVDGIYIAGVIAAGNN 303 Query: 302 ANEVFIETGRGHGKLIADHI 321 ANE+FIE GR HG LIA H+ Sbjct: 304 ANEIFIENGRHHGGLIASHL 323 >C3IYR1.1 C3IYR1_9BACI FAD-dependent pyridine nucleotide-disulphide oxidoreductase Length = 330 Score = 325 bits (834), Expect = 3e-87, Method: Compositional matrix adjust. Identities = 162/324 (50%), Positives = 215/324 (66%), Gaps = 7/324 (2%) Query: 2 KDVIIIGAGPCGLSAAIECQRRGLSSLIIEKNFIVHSIYLYPTNMQFFSTTALLEIGDVP 61 + +II+G GPCGL+AAI Q G S L+IEK IVHSIY +PT+ FFST+ LEIG VP Sbjct: 4 EKIIIVGGGPCGLAAAIALQDAGFSPLVIEKGNIVHSIYRFPTHQTFFSTSDRLEIGGVP 63 Query: 62 FTSPNDKPFRHEALVYYRRAAEQHGLDIAAYEEALSVKPLEDGTFAVHTRNKRGETYTRT 121 F + N KP R++AL YYR + + + +EE +VKP EDG F + T T Sbjct: 64 FITENQKPTRNQALAYYREVVVRKQVRVNTFEEVKAVKPQEDGAFLIETTKG-----TYR 118 Query: 122 AANVIISTGYFDQPNLIGIPGEELPKVTHYFGEAHPYTGMKVTVIGGSNSAVDAALELLR 181 A V+I+TGY+D PN + +PGEELPKV HYF EAHPY VIGG NS+VDAA+EL++ Sbjct: 119 AQYVVIATGYYDHPNYMNVPGEELPKVMHYFKEAHPYFNTDCVVIGGKNSSVDAAMELVK 178 Query: 182 VGAEVDMVYRGASISENIKPWVRPIFESMVAKDKIVLHLESRVTEITPGSVHITGRNGEV 241 GA V ++YRG S++IKPW+ P F+S+V K I + + V IT +V + +GE Sbjct: 179 AGARVTVLYRGNEYSKSIKPWILPEFDSLVKKGVIRMEFRAHVKAITEDAV-VYEVDGET 237 Query: 242 KELDNDFVLALTGFRPSRTLLSSAGVSMDETQEKPAFNPSTMESNVPGLYIAGVIASGRN 301 K + NDFV A+TG+RP L + GV +D +P ++P TME+NVPG++IAGVIA+G + Sbjct: 238 KTIKNDFVFAMTGYRPDHRFLMNIGVQIDPESGRPHYDPETMETNVPGVFIAGVIAAGND 297 Query: 302 ANEVFIETGRGHGKLIADHIISKR 325 ANE+FIE GR HG IA I+KR Sbjct: 298 ANEIFIENGRFHGDAIA-ACIAKR 320 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,965,195,876 Number of extensions: 82502927 Number of successful extensions: 294015 Number of sequences better than 1.0e-05: 677 Number of HSP's gapped: 294546 Number of HSP's successfully gapped: 677 Length of query: 328 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 192 Effective length of database: 1,779,438,163 Effective search space: 341652127296 Effective search space used: 341652127296 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)