BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0450.0013.p (223 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KH31.1 B3KH31_9BACI HAD-superfamily hydrolase, subfamily IA, v... 152 2e-35 B9XL18.1 B9XL18_9BACT HAD-superfamily hydrolase, subfamily IA, v... 127 5e-28 Q2BCC5.1 Q2BCC5_9BACI Hydrolase (HAD superfamily) protein 126 1e-27 C2N826.1 C2N826_BACCE Hydrolase (HAD superfamily) 118 3e-25 C3ES40.1 C3ES40_BACTK Hydrolase (HAD superfamily) 118 5e-25 >B3KH31.1 B3KH31_9BACI HAD-superfamily hydrolase, subfamily IA, variant 1 Length = 232 Score = 152 bits (384), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 78/219 (35%), Positives = 119/219 (54%) Query: 4 VQALLFDLDNTLMDRDHTFRSFSRKFVQDFLGHMDPEEAERTVEDIIVRDADGYRDKDGF 63 V+A++FDLD TL DR +F ++ + + D + D +GYRDK Sbjct: 3 VKAIIFDLDETLTDRRAAINTFIKRLIARYFPDSDEAAQMMIAKRFKEADHNGYRDKREV 62 Query: 64 FAELSDVLPWRKKVTAADIRAYYDRTYITHGALMKHAVEILEYCRERGYTLGLVTNGKKD 123 + L + LPW A + +++ + M V +L + G LG++TNG Sbjct: 63 YEMLVEQLPWVNPPEADEYLSFFRGEIASCIQPMDQLVSVLRELKTWGLKLGIITNGTVQ 122 Query: 124 IQYGKIDLLKLRGYFKAIVISGEAGISKPDPEIYRLALERLGTKAEETLFIGDHPVNDIW 183 +Q GKI L +R YF +IVIS EAG+ KP+P I+ AL +L E ++GDHP ND+ Sbjct: 123 VQEGKIHQLGIREYFDSIVISEEAGVKKPEPAIFTRALSQLHVMPSEAWYVGDHPHNDVI 182 Query: 184 GAAKAGMETIWLKRNHPWDEKLDAHPWKTIHELDELKSI 222 GAA+ G++ IW R+ WD +D P++TIH+L++L I Sbjct: 183 GAAQCGIKAIWYTRDGGWDASMDVKPYRTIHKLEQLIPI 221 >B9XL18.1 B9XL18_9BACT HAD-superfamily hydrolase, subfamily IA, variant 1 Length = 231 Score = 127 bits (320), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 72/218 (33%), Positives = 115/218 (52%), Gaps = 6/218 (2%) Query: 7 LLFDLDNTLMDRDHTFRSFSRKFVQDFLGHMDPEEAERTVEDIIVRDADGYRDKDGFFAE 66 + FDLDNTL+DRD +R + +F+Q +P ++ +E II+ D G+RD+ FF+ Sbjct: 6 IFFDLDNTLLDRDAAWRIYWSQFIQQNPAIFNPH-SQSALEQIIIEDQHGWRDRAAFFSW 64 Query: 67 LSDVLPWRKKVTAADIRAYYD-RTYITHGALMKHAVEILEYCRERGYTLGLVTNGKKDIQ 125 L+ P K+ + + R + ++ V L C ++ Y L LV+NG +Q Sbjct: 65 LTQSFP---KLDQPPMALWEQCRQQLGKLSVPYPGVRELLICLKKTYPLTLVSNGSSTVQ 121 Query: 126 YGKIDLLKLRGYFKAIVISGEAGISKPDPEIYRLALERLGTKAEETLFIGDHPVNDIWGA 185 K+ L +F I ISGE G+ KPDP I++ AL+ E LF+GD PV D++GA Sbjct: 122 RMKLLHSGLAVFFDHIFISGEVGVDKPDPGIFKAALKESNYAPENILFVGDDPVRDVFGA 181 Query: 186 AKAGMETIWLKRNHPWDEKLDAHPWKTIHELDELKSIL 223 G++T W+ + W + P + E+ EL ++L Sbjct: 182 GSLGLQTCWISHGNSWTAN-QSKPDFILREVTELPALL 218 >Q2BCC5.1 Q2BCC5_9BACI Hydrolase (HAD superfamily) protein Length = 274 Score = 126 bits (317), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 77/219 (35%), Positives = 120/219 (54%), Gaps = 11/219 (5%) Query: 4 VQALLFDLDNTLMDRDHTFRSFSRKFVQ---DFLGHMDPEEAERTVEDIIVRDADGYRDK 60 ++A+ FDLD TL+DRD + + FS + Q D L H+ +++ DA GY K Sbjct: 1 MKAVFFDLDGTLLDRDASIKEFSIQQYQRYYDKLSHIPQAVFTNRFKEL---DARGYVWK 57 Query: 61 DGFFAELSDVLPWRKKVTAADIRAYYDRTYITHGALMKHAVEILEYCRERGYTLGLVTNG 120 D + ++ + + + A D+ Y + H +E L R+R +G+++NG Sbjct: 58 DKVYQQMEAEFTF-QGIAAEDLLMDYLNHFHQSCVPFPHLIETLSDLRDRQIKMGIISNG 116 Query: 121 KKDIQYGKIDLLKLRGYFKAIVISGEAGISKPDPEIYRLALERLGTKAEETLFIGDHPVN 180 + Q I L++ YF I+IS + GISKPDP+I+ A++RL E++F+GDHPVN Sbjct: 117 RWGFQMASIQALQVEEYFGVILISEQEGISKPDPQIFHRAMDRLCVSPYESVFVGDHPVN 176 Query: 181 DIWGAAKAGMETIWLKRNHPWDEKLDAHPWKTIHELDEL 219 DI G+ + GME +W KR+ W+ A WK I +L EL Sbjct: 177 DIEGSKRCGMEAVW-KRDPNWNNT--AVKWK-IDDLKEL 211 >C2N826.1 C2N826_BACCE Hydrolase (HAD superfamily) Length = 221 Score = 118 bits (296), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 69/202 (34%), Positives = 105/202 (51%), Gaps = 10/202 (4%) Query: 4 VQALLFDLDNTLMDRDHTFRSFSRKFVQDFLGHMDPEEAERTVEDIIVRDADGYRDKDGF 63 ++A+LFDLD TL+DR + F + F H+ E + D +GY KD Sbjct: 2 IRAILFDLDGTLLDRRQSLEQFICEQYNRFASHLKSIEKSEYCSRFLELDNNGYTWKDKV 61 Query: 64 FAELSDVLPWRKKVTAADIRAYYDRTYIT----HGALMKHAVEILEYCRERGYTLGLVTN 119 +A L L T + +D YIT H K+ E+L+ ++R +G++TN Sbjct: 62 YATL---LSEYNITTLTQEQLLHD--YITNFQHHCIPFKNTHELLQQLKQRNIKIGIITN 116 Query: 120 GKKDIQYGKIDLLKLRGYFKAIVISGEAGISKPDPEIYRLALERLGTKAEETLFIGDHPV 179 G + Q + L + Y AI++S GI KP PEI+ AL+RL KAEE L++GDHP Sbjct: 117 GFTEFQMSNLRALHIHTYTNAILVSEAEGIKKPHPEIFERALQRLDVKAEECLYVGDHPE 176 Query: 180 NDIWGAAKAGMETIWLKRNHPW 201 ND+ G+ + G+ +W K++ W Sbjct: 177 NDVLGSEQVGILGVW-KKDSFW 197 >C3ES40.1 C3ES40_BACTK Hydrolase (HAD superfamily) Length = 221 Score = 118 bits (295), Expect = 5e-25, Method: Compositional matrix adjust. Identities = 68/202 (33%), Positives = 105/202 (51%), Gaps = 10/202 (4%) Query: 4 VQALLFDLDNTLMDRDHTFRSFSRKFVQDFLGHMDPEEAERTVEDIIVRDADGYRDKDGF 63 ++A+LFDLD TL+DR + F + F H+ E + + D +GY KD Sbjct: 2 IRAILFDLDGTLLDRRQSLEQFICEQYNRFASHLKSIEKSKYCSRFLELDNNGYTWKDKV 61 Query: 64 FAELSDVLPWRKKVTAADIRAYYDRTYIT----HGALMKHAVEILEYCRERGYTLGLVTN 119 +A L L T + +D YIT H K+ E+L+ ++R +G++TN Sbjct: 62 YATL---LSEYNITTLTQEQLLHD--YITNFQHHCIPFKNTHELLQQLKQRNIKIGIITN 116 Query: 120 GKKDIQYGKIDLLKLRGYFKAIVISGEAGISKPDPEIYRLALERLGTKAEETLFIGDHPV 179 G + Q + L + Y I++S GI KP PEI+ AL+RL KAEE L++GDHP Sbjct: 117 GFTEFQMSNLRALHIHTYTNTILVSEAEGIKKPHPEIFERALQRLDVKAEECLYVGDHPE 176 Query: 180 NDIWGAAKAGMETIWLKRNHPW 201 ND+ G+ + G+ +W K++ W Sbjct: 177 NDVLGSEQVGILGVW-KKDSFW 197 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.140 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,395,409,742 Number of extensions: 58138649 Number of successful extensions: 161898 Number of sequences better than 1.0e-05: 1915 Number of HSP's gapped: 161418 Number of HSP's successfully gapped: 1915 Length of query: 223 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 92 Effective length of database: 1,826,543,238 Effective search space: 168041977896 Effective search space used: 168041977896 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)