BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0450.0002.p (343 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0ZH48.1 C0ZH48_BREBN Putative uncharacterized protein 230 2e-58 Q2B035.1 Q2B035_9BACI Putative uncharacterized protein 218 9e-55 B2A7I2.1 B2A7I2_NATTJ Metal dependent phosphohydrolase 213 2e-53 Q9K6D5.1 Q9K6D5_BACHD BH3794 protein 209 4e-52 B3KBK2.1 B3KBK2_9BACI Metal dependent phophohydrolase 201 6e-50 >C0ZH48.1 C0ZH48_BREBN Putative uncharacterized protein Length = 360 Score = 230 bits (586), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 115/284 (40%), Positives = 172/284 (60%), Gaps = 2/284 (0%) Query: 60 SEAFYQLAVDDCTAAMENIFDQLRYTKSKRLPMLELKNEVIPFIQQVSEKNDFFGILAAL 119 ++ +Q + D + ++N+ R +P++E++ V P I + ++ L Sbjct: 79 AKPVFQESFDKAVSTLKNLMT--RVQGGNNIPVMEVREVVTPIITEFQQQPQVLLSLRRF 136 Query: 120 QTKDDYTYRHNVAVGILSTLLGKWLKLDPEELGMLTIAATLHDIGKIMIPAEILTKPGPL 179 D Y Y H VAVGI+S ++ KW+K+ +E + +A TL DIGK I IL KPG L Sbjct: 137 AKMDSYAYEHAVAVGIISYMIAKWVKVPEKEWMQVALAGTLLDIGKTKIDRRILQKPGKL 196 Query: 180 TDEEFDIMKKHTTHGYELVRDTPGTTHMQALVALQHHERMDGSGYPFGVLGHRITDFSKI 239 T +EF+ MKKHT +GY++++ + G + AL ALQHHER DGSGYP G+ G ++ +SKI Sbjct: 197 TPDEFEEMKKHTVYGYQIIKSSHGLSEGVALAALQHHEREDGSGYPLGLPGSKLHLYSKI 256 Query: 240 VAVTDIFHAMTSDRFYSEAAPLYEVLTQMEDYSFGKLDPYICGVFISKLMQSMIGNEVLL 299 VAV D++HAM+SDR + +A Y+V+ Q+ SFGKLDP I F+ + Q +G V L Sbjct: 257 VAVADVYHAMSSDRVHQKALSPYQVVEQLVQDSFGKLDPTIVRSFVEGITQFAVGTLVEL 316 Query: 300 TDGRMGKIIMILAHDPLRPLVNIGEDFIDLSKNRHLGIVRVIPQ 343 +DG +GKI+ + P RP+V G ++L + RHL IVRV+ Q Sbjct: 317 SDGTIGKIVFTDRNHPTRPMVETGGKIVNLVEARHLSIVRVMEQ 360 >Q2B035.1 Q2B035_9BACI Putative uncharacterized protein Length = 325 Score = 218 bits (554), Expect = 9e-55, Method: Compositional matrix adjust. Identities = 111/274 (40%), Positives = 164/274 (59%), Gaps = 2/274 (0%) Query: 68 VDDCTAAMENIFDQLRYTKSKRLPMLELKNEVIPFIQQVSEKNDFFGILAALQTKDDYTY 127 +D +E+IF R K + + +K +++P I+ +++ + L KD YT Sbjct: 54 IDHAVGQVEDIFAGARLGKG--VGITAIKKDILPVIRTLADIPGVSHVFHQLDRKDRYTS 111 Query: 128 RHNVAVGILSTLLGKWLKLDPEELGMLTIAATLHDIGKIMIPAEILTKPGPLTDEEFDIM 187 H V V +++ ++G+WL + + L + + LHDIGKI IP EIL+KPG LT E++ M Sbjct: 112 SHAVCVSMIAGMIGRWLGIGGALMEDLLLGSILHDIGKIKIPDEILSKPGKLTLSEYEEM 171 Query: 188 KKHTTHGYELVRDTPGTTHMQALVALQHHERMDGSGYPFGVLGHRITDFSKIVAVTDIFH 247 K H+ +GYE+VRD + AL+ LQHHER DG GYP G+ G I +KI A+ D+FH Sbjct: 172 KLHSVYGYEMVRDLDAVSPKAALIVLQHHEREDGRGYPLGLKGREIEHLAKITAIADVFH 231 Query: 248 AMTSDRFYSEAAPLYEVLTQMEDYSFGKLDPYICGVFISKLMQSMIGNEVLLTDGRMGKI 307 AM+S R Y EA P Y+V+ +M+D +FG +P +FI LM S+ G+ V LTD G+I Sbjct: 232 AMSSARIYHEALPFYQVMRKMKDGAFGSFEPGFMLIFIQNLMNSLAGSRVRLTDQSEGRI 291 Query: 308 IMILAHDPLRPLVNIGEDFIDLSKNRHLGIVRVI 341 +MI +DPL LV + IDL +R L I R++ Sbjct: 292 LMIHPYDPLSALVQTEDRLIDLRYSRGLQIERIL 325 >B2A7I2.1 B2A7I2_NATTJ Metal dependent phosphohydrolase Length = 346 Score = 213 bits (542), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 114/337 (33%), Positives = 198/337 (58%), Gaps = 12/337 (3%) Query: 13 GKQLVDNLFNHNGVFVLPAMTLLSSEHVRLITQHRI--ILQPHDVIQLD-----SEAFYQ 65 G +L ++ + +G ++P +LS + ++ + I +L + Q+ S+A ++ Sbjct: 12 GMKLSKDVNSKSGATLVPKGVILSKKTIKFLINWGIKSVLIEEEEEQITRGRGHSQAKFK 71 Query: 66 LAVDDCTAAMENIFDQLRYTKSKRL-PMLELKNEVIPFIQQVSEKNDFFGILAALQTKDD 124 + T + + + L+ +K+ L + E+ E++ + ++ +L ++ KD Sbjct: 72 KEYFETTEKVISFMEGLKGSKAIELDEVKEIAQELVTYSDDINS----VKLLTEVKDKDY 127 Query: 125 YTYRHNVAVGILSTLLGKWLKLDPEELGMLTIAATLHDIGKIMIPAEILTKPGPLTDEEF 184 YTY+H++ VGI ++LLG+WL ++ L++A LHDIGK IP E+L KP LTD+EF Sbjct: 128 YTYQHSINVGIYASLLGRWLGYCTNDIKNLSLAGLLHDIGKTQIPDEVLLKPESLTDKEF 187 Query: 185 DIMKKHTTHGYELVRDTPGTTHMQALVALQHHERMDGSGYPFGVLGHRITDFSKIVAVTD 244 +I+K H +GYEL++ + + LQHHER +GSGYPFG+ G +I +SKI++V D Sbjct: 188 EIIKNHPIYGYELLKPCEDLSPEINIAVLQHHERENGSGYPFGITGEKIHSYSKIISVVD 247 Query: 245 IFHAMTSDRFYSEAAPLYEVLTQMEDYSFGKLDPYICGVFISKLMQSMIGNEVLLTDGRM 304 IF A+TS+R Y +E + ++ D SF L+P I F ++ + +G+ V+ DG + Sbjct: 248 IFDAITSNRVYKGRNSPFEAIREVRDKSFQDLNPEIAYTFFKRISEFFVGSTVIFNDGSI 307 Query: 305 GKIIMILAHDPLRPLVNIGEDFIDLSKNRHLGIVRVI 341 GKI+ + +DP RPLV I + F+DLSK+++ I+ V+ Sbjct: 308 GKIVFLNKNDPTRPLVQIEDKFVDLSKHKNREIIDVM 344 >Q9K6D5.1 Q9K6D5_BACHD BH3794 protein Length = 231 Score = 209 bits (532), Expect = 4e-52, Method: Compositional matrix adjust. Identities = 102/212 (48%), Positives = 139/212 (65%) Query: 118 ALQTKDDYTYRHNVAVGILSTLLGKWLKLDPEELGMLTIAATLHDIGKIMIPAEILTKPG 177 ++ KDDYTYRH V V ILS L+G WLKLD L LT+ A LHDIGK+ IP IL KPG Sbjct: 3 VIKAKDDYTYRHTVGVSILSRLIGTWLKLDEVILKELTLGAVLHDIGKVEIPDAILNKPG 62 Query: 178 PLTDEEFDIMKKHTTHGYELVRDTPGTTHMQALVALQHHERMDGSGYPFGVLGHRITDFS 237 LT +E+ I++ HT GY +++++ + AL+AL+HHER DG+GYP+ + I S Sbjct: 63 ALTKDEYRIIQDHTVKGYRIIQESGIYSDTVALMALEHHEREDGTGYPYQKTRNEIHLLS 122 Query: 238 KIVAVTDIFHAMTSDRFYSEAAPLYEVLTQMEDYSFGKLDPYICGVFISKLMQSMIGNEV 297 KI+AV D+FHAMTSDR Y + LY+VL QM FGKL+P I F+ + M+ IG++ Sbjct: 123 KILAVADVFHAMTSDRIYRKGMALYDVLLQMRQDRFGKLEPRITDFFVRRFMEQCIGSKA 182 Query: 298 LLTDGRMGKIIMILAHDPLRPLVNIGEDFIDL 329 L DG +I+ I +P+ P+V G ++IDL Sbjct: 183 RLNDGSRVEIVFIPYENPICPIVKQGTNYIDL 214 >B3KBK2.1 B3KBK2_9BACI Metal dependent phophohydrolase Length = 348 Score = 201 bits (512), Expect = 6e-50, Method: Compositional matrix adjust. Identities = 126/344 (36%), Positives = 183/344 (53%), Gaps = 22/344 (6%) Query: 13 GKQLVDNLFNHNGVFVLPAMTLLSSEHVRLITQH---RIILQPHDV-IQLD-----SEAF 63 G Q+ + +N NGV VL LL SE + + QH I +QP + + LD S++F Sbjct: 11 GDQIQQDAYNTNGVHVLQKGALLRSEDISKLLQHGIDYIEIQPRALSVSLDPLSEISDSF 70 Query: 64 ------YQLAVDDCTAAMENIFDQLRYTKSKRLPMLELKNEVIPFIQQVSEKNDFFGILA 117 + LA D C+A ++ +S + + P + ++E D +L Sbjct: 71 NKVKPHFNLAFDGCSALFTEASQSGKFNESVVDDVFQ------PLVGSLNEHTDVVSLLL 124 Query: 118 ALQTKDDYTYRHNVAVGILSTLLGKWLKLDPEELGMLTIAATLHDIGKIMIPAEILTKPG 177 DDYTYRH++ VG+LS + W+ +E + A LHDIGK I +IL KPG Sbjct: 125 LFNGTDDYTYRHSIQVGMLSYYIADWMGYSGKEAYEIGKAGYLHDIGKSKISRDILDKPG 184 Query: 178 PLTDEEFDIMKKHTTHGYELVRDTPGTTHMQALVALQHHERMDGSGYPFGVLGHRITDFS 237 LT EEF+ +K HT +GYE++ ++ + A+VALQHHER D SGYP + +I +S Sbjct: 185 KLTPEEFEEVKLHTIYGYEMIAESMDDK-VTAMVALQHHERDDRSGYPRALHADQIHPYS 243 Query: 238 KIVAVTDIFHAMTSDRFYSEAAPLYEVLTQMEDYSFGKLDPYICGVFISKLMQSMIGNEV 297 I AV D++ AMT++R Y L VL ++ SFGKL+ FI +++ + IG +V Sbjct: 244 HICAVADVYSAMTTNRVYQSKQQLLTVLRELYSLSFGKLNGKPTHAFIQQMLPNFIGKKV 303 Query: 298 LLTDGRMGKIIMILAHDPLRPLVNIGEDFIDLSKNRHLGIVRVI 341 LLT G G I+M D RPLV + FIDLSK H I ++ Sbjct: 304 LLTTGESGIIVMNHPSDYFRPLVKTDDRFIDLSKEYHTEIQEIL 347 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.140 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,045,679,208 Number of extensions: 82542658 Number of successful extensions: 201779 Number of sequences better than 1.0e-05: 2435 Number of HSP's gapped: 199774 Number of HSP's successfully gapped: 2436 Length of query: 343 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 207 Effective length of database: 1,779,438,163 Effective search space: 368343699741 Effective search space used: 368343699741 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)