BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0449.0011.p (273 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q65NT0.1 Q65NT0_BACLD Putative uncharacterized protein 338 3e-91 A8F9L5.1 A8F9L5_BACP2 Amidase 305 2e-81 B4AMP3.1 B4AMP3_BACPU Amidase 302 2e-80 C1PAQ8.1 C1PAQ8_BACCO Amidase 295 2e-78 Q8ESC9.1 Q8ESC9_OCEIH 6-aminohexanoate-cyclic-dimer hydrolase 264 7e-69 >Q65NT0.1 Q65NT0_BACLD Putative uncharacterized protein Length = 499 Score = 338 bits (867), Expect = 3e-91, Method: Compositional matrix adjust. Identities = 163/272 (59%), Positives = 204/272 (75%) Query: 1 MLDLLQTLQPEAAFQTPLYPGVYRDDLMKPLQRKLRVAYTTASPVGTPVSGEAVAAVLKT 60 +LD+LQT+QPEAAFQTPL+PG Y +D+ K L+R L++A++ SPVGTPVS EA AV +T Sbjct: 225 LLDILQTIQPEAAFQTPLFPGQYEEDMKKGLERPLKIAFSVESPVGTPVSDEAKQAVRRT 284 Query: 61 VKWLQAEGHEVEEKLSPVNGVWLMENYYTMNAGEVAAMFMSLENDMGRSIAAHEVDIVTW 120 V+WL+A+GH VEE+ + V+G+ LMENYY MN+GE++AM + +E +GR+I +V+IVTW Sbjct: 285 VRWLEAQGHHVEEQTNGVDGIRLMENYYLMNSGEISAMILGMEKTLGRAITVQDVEIVTW 344 Query: 121 VLGEAGKKVTAAEFVHSLNEWDVAAAQMAGLLSRYDLYITPANADSAPEIGELTQQPEEI 180 VL EAGKKV+AAEF SL WD AAAQMA YDLY+TPA A AP++GELT +E Sbjct: 345 VLNEAGKKVSAAEFTASLASWDRAAAQMAAFHETYDLYVTPATAYPAPKVGELTHTEKEA 404 Query: 181 EALLHISELPKEAQQRMIYEMFEPSLTYTPFTQLANLTGQPAMSVPLHLTPAGLPIGVQV 240 EALL +SEL K QQ +IY+MF SLTYTPFTQLANLTGQPAMSVP+HLT GLP+GVQV Sbjct: 405 EALLKVSELEKIEQQSLIYQMFLKSLTYTPFTQLANLTGQPAMSVPVHLTNEGLPLGVQV 464 Query: 241 MASKGREDXXXXXXXXXXXXXXWVGMKGNPLF 272 A KG+E W+GMKGNP+ Sbjct: 465 TAPKGKEHWLLRTAHLLEQSDLWIGMKGNPML 496 >A8F9L5.1 A8F9L5_BACP2 Amidase Length = 495 Score = 305 bits (782), Expect = 2e-81, Method: Compositional matrix adjust. Identities = 151/265 (56%), Positives = 188/265 (70%), Gaps = 1/265 (0%) Query: 1 MLDLLQTLQPEAAFQTPLYPGVYRDDLMKPLQRKLRVAYTTASPVGTPVSGEAVAAVLKT 60 +LDLLQ +QPEAAFQTPLY G Y++DL+K +R+AY+ SPVGT VS EA AV +T Sbjct: 226 LLDLLQVIQPEAAFQTPLYAGSYQEDLVK-RTSSMRIAYSVESPVGTKVSEEAKQAVQQT 284 Query: 61 VKWLQAEGHEVEEKLSPVNGVWLMENYYTMNAGEVAAMFMSLENDMGRSIAAHEVDIVTW 120 VKWL +GH+VEE ++G+ LM+ YY MN+GE++A+FMSL +GR + E DIV W Sbjct: 285 VKWLSDQGHQVEEAKPAIDGIHLMQQYYVMNSGEMSALFMSLNRSLGRPVNPEETDIVAW 344 Query: 121 VLGEAGKKVTAAEFVHSLNEWDVAAAQMAGLLSRYDLYITPANADSAPEIGELTQQPEEI 180 VL EAGK VTAA + SL+ WD+AAAQMA YDL++TPA A SAP++GEL EEI Sbjct: 345 VLAEAGKNVTAAAYTESLDAWDMAAAQMASFHQTYDLFVTPATAYSAPKVGELMHSKEEI 404 Query: 181 EALLHISELPKEAQQRMIYEMFEPSLTYTPFTQLANLTGQPAMSVPLHLTPAGLPIGVQV 240 LL +S L +AQQ +IY+MF SLTYTPFTQLANLTGQP+MSVP+HLT AG+P+GVQV Sbjct: 405 TQLLRVSSLSMQAQQDLIYDMFLKSLTYTPFTQLANLTGQPSMSVPVHLTEAGMPLGVQV 464 Query: 241 MASKGREDXXXXXXXXXXXXXXWVG 265 A KG+ED W G Sbjct: 465 TAPKGKEDWLLRLAAEMETSSIWKG 489 >B4AMP3.1 B4AMP3_BACPU Amidase Length = 495 Score = 302 bits (774), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 150/265 (56%), Positives = 187/265 (70%), Gaps = 1/265 (0%) Query: 1 MLDLLQTLQPEAAFQTPLYPGVYRDDLMKPLQRKLRVAYTTASPVGTPVSGEAVAAVLKT 60 +LDLLQ +QPEAAFQTPLY G Y++DL+K +R+AY+ SPVGT VS EA AV +T Sbjct: 226 LLDLLQVIQPEAAFQTPLYDGSYQEDLVK-RTSSMRIAYSVESPVGTKVSEEAKQAVQQT 284 Query: 61 VKWLQAEGHEVEEKLSPVNGVWLMENYYTMNAGEVAAMFMSLENDMGRSIAAHEVDIVTW 120 VKWL +GH+VEE ++G+ LM+ YY MN+GE++A+F SL +GR + E DIV W Sbjct: 285 VKWLSDQGHQVEEAGPAIDGIHLMQQYYVMNSGEMSALFTSLNRSLGRPVNPEETDIVAW 344 Query: 121 VLGEAGKKVTAAEFVHSLNEWDVAAAQMAGLLSRYDLYITPANADSAPEIGELTQQPEEI 180 VL EAGK VTAA + SL+ WD+AAAQMA YDL++TPA A SAP++GEL EEI Sbjct: 345 VLAEAGKNVTAAAYTESLDAWDMAAAQMASFHQTYDLFVTPATAYSAPKVGELMHSKEEI 404 Query: 181 EALLHISELPKEAQQRMIYEMFEPSLTYTPFTQLANLTGQPAMSVPLHLTPAGLPIGVQV 240 LL +S L +AQQ +IY+MF SLTYTPFTQLANLTGQP+MSVP+HLT AG+P+GVQV Sbjct: 405 TELLRVSSLSMQAQQDLIYDMFLKSLTYTPFTQLANLTGQPSMSVPVHLTKAGMPLGVQV 464 Query: 241 MASKGREDXXXXXXXXXXXXXXWVG 265 A KG+ED W G Sbjct: 465 TAPKGKEDWLLRLAAEMETSSIWKG 489 >C1PAQ8.1 C1PAQ8_BACCO Amidase Length = 495 Score = 295 bits (756), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 149/272 (54%), Positives = 192/272 (70%), Gaps = 1/272 (0%) Query: 1 MLDLLQTLQPEAAFQTPLYPGVYRDDLMKPLQRKLRVAYTTASPVGTPVSGEAVAAVLKT 60 +LD+LQT++P AAFQTPL+PG Y +L + +K R+A++ SPVGTPVS +A AV KT Sbjct: 225 LLDVLQTVEPAAAFQTPLFPG-YVKELERSGTKKYRIAFSVESPVGTPVSRDAKQAVYKT 283 Query: 61 VKWLQAEGHEVEEKLSPVNGVWLMENYYTMNAGEVAAMFMSLENDMGRSIAAHEVDIVTW 120 KWL+ EGH VEE + V+G LMENYY MN GE+AA LE+ + R + +++ V+W Sbjct: 284 AKWLEQEGHLVEEAKNGVDGRRLMENYYLMNCGEMAAELSDLESALRRPLTEKDMEPVSW 343 Query: 121 VLGEAGKKVTAAEFVHSLNEWDVAAAQMAGLLSRYDLYITPANADSAPEIGELTQQPEEI 180 VL AGK VTAAE+ SL EWD AAAQMA YDLYITP+ A AP+IGELTQ +E Sbjct: 344 VLYAAGKNVTAAEYAKSLAEWDKAAAQMAAFHETYDLYITPSTAFPAPKIGELTQTGKER 403 Query: 181 EALLHISELPKEAQQRMIYEMFEPSLTYTPFTQLANLTGQPAMSVPLHLTPAGLPIGVQV 240 + L ++ LPKE QQ ++Y+MFEPSLT++PFTQLANLTGQPA+S+P+H+T AGLP+GVQ+ Sbjct: 404 DRLFGVTGLPKEKQQALVYDMFEPSLTFSPFTQLANLTGQPAISLPVHVTAAGLPLGVQL 463 Query: 241 MASKGREDXXXXXXXXXXXXXXWVGMKGNPLF 272 +A KG E WVGM+GNP Sbjct: 464 VAPKGNEHWLLDVAYALEQSELWVGMQGNPCL 495 >Q8ESC9.1 Q8ESC9_OCEIH 6-aminohexanoate-cyclic-dimer hydrolase Length = 502 Score = 264 bits (674), Expect = 7e-69, Method: Compositional matrix adjust. Identities = 134/273 (49%), Positives = 180/273 (65%), Gaps = 1/273 (0%) Query: 1 MLDLLQTLQPEAAFQTPLYPGVYRDDLMKPLQRKLRVAYTTASPVGTPVSGEAVAAVLKT 60 MLD LQ ++P AAFQTPL+ G Y +D+ K ++ LR+AY+T SPVGTPVS +A AV Sbjct: 226 MLDQLQVVEPHAAFQTPLFAGGYLNDIEKSPEKSLRIAYSTESPVGTPVSEDAKEAVNNL 285 Query: 61 VKWLQAEGHEVEEKLSPVNGVWLMENYYTMNAGEVAAMFMSLENDMGRSIAAHEVDIVTW 120 V+ L EGH++EE +P++G LM +YY MN+GE+ A + +EN + R + +++I +W Sbjct: 286 VQLLSNEGHDLEEVQAPIDGRQLMNDYYIMNSGEMNATILGIENMLNRKLTDDDMEIESW 345 Query: 121 VLGEAGKKVTAAEFVHSLNEWDVAAAQMAGLLSRYDLYITPANADSAPEIGELTQQPEEI 180 +L AG+ V+AA + SL+ WD+AA +MA YD YITP+ A SAP +GELT E+ Sbjct: 346 LLHRAGESVSAAAYSQSLSSWDLAAEKMAYFHQTYDFYITPSTAYSAPRVGELTMTKEKQ 405 Query: 181 EALLH-ISELPKEAQQRMIYEMFEPSLTYTPFTQLANLTGQPAMSVPLHLTPAGLPIGVQ 239 E I + +Q +IY+MF PSLTYTPFTQLANLTGQPAMS+PL+LT GLP+GVQ Sbjct: 406 EDYKECIEKASAREKQSIIYDMFLPSLTYTPFTQLANLTGQPAMSLPLYLTEQGLPLGVQ 465 Query: 240 VMASKGREDXXXXXXXXXXXXXXWVGMKGNPLF 272 VMA+KG E WV + NP F Sbjct: 466 VMANKGEEHRLLRFARYLEQSNLWVKVNENPYF 498 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.131 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,591,172,212 Number of extensions: 63928139 Number of successful extensions: 172560 Number of sequences better than 1.0e-05: 338 Number of HSP's gapped: 172205 Number of HSP's successfully gapped: 338 Length of query: 273 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 139 Effective length of database: 1,798,280,193 Effective search space: 249960946827 Effective search space used: 249960946827 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)