BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0449.0010.p (191 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B4AMP3.1 B4AMP3_BACPU Amidase 207 3e-52 A8F9L5.1 A8F9L5_BACP2 Amidase 202 1e-50 Q65NT0.1 Q65NT0_BACLD Putative uncharacterized protein 182 9e-45 C1PAQ8.1 C1PAQ8_BACCO Amidase 174 3e-42 Q8ESC9.1 Q8ESC9_OCEIH 6-aminohexanoate-cyclic-dimer hydrolase 156 8e-37 >B4AMP3.1 B4AMP3_BACPU Amidase Length = 495 Score = 207 bits (528), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 97/156 (62%), Positives = 123/156 (78%), Gaps = 3/156 (1%) Query: 1 MTLSAYSTYDALGLAALVRAREVSPRELVEAAFARLDEVNPLLNAVVRTRREAALKEADA 60 M + Y TYDA+GLAALVR ++V+P ELV+AAFARL+EVNP LNA+++TR++ LKE Sbjct: 3 MNVKEYMTYDAIGLAALVRNKQVTPDELVQAAFARLNEVNPELNALIQTRQDQVLKEIKT 62 Query: 61 MPPDDPSRPFAGVPFLLKDISQAIGGEPLTSGALLLKDNIAKRDSNYVARIRAGGFIPLG 120 + S+PFAGVPF+LK+ISQ + EPLT+GA LLKD AK DS++V R++ GF+ +G Sbjct: 63 L---HTSQPFAGVPFVLKNISQGLENEPLTAGAALLKDVKAKTDSHFVQRLKQAGFLMMG 119 Query: 121 HTNTPEFGLKNITEPLLHGPARNPWSTDCSPGGSSG 156 HTNTPEFGL+N+TEP LHGP RNPW D SPGGSSG Sbjct: 120 HTNTPEFGLRNVTEPALHGPTRNPWHPDYSPGGSSG 155 >A8F9L5.1 A8F9L5_BACP2 Amidase Length = 495 Score = 202 bits (515), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 95/156 (60%), Positives = 121/156 (77%), Gaps = 3/156 (1%) Query: 1 MTLSAYSTYDALGLAALVRAREVSPRELVEAAFARLDEVNPLLNAVVRTRREAALKEADA 60 M + Y TYDA GLAALVR ++V+P ELV+AAFARL+EVNP LNA+ +TR++ +KE Sbjct: 3 MNVKEYMTYDATGLAALVRNKQVTPDELVQAAFARLNEVNPELNALTQTRQDQVVKEMKT 62 Query: 61 MPPDDPSRPFAGVPFLLKDISQAIGGEPLTSGALLLKDNIAKRDSNYVARIRAGGFIPLG 120 + ++PFAGVPF+LK+ISQ + EPLT+GA LLKD AK DS++V R++ GF+ +G Sbjct: 63 L---HTNQPFAGVPFVLKNISQGLENEPLTAGAALLKDVKAKTDSHFVQRLKQAGFLMVG 119 Query: 121 HTNTPEFGLKNITEPLLHGPARNPWSTDCSPGGSSG 156 HTNTPEFGL+N+TEP LHGP RNPW D SPGGSSG Sbjct: 120 HTNTPEFGLRNVTEPALHGPTRNPWHPDYSPGGSSG 155 >Q65NT0.1 Q65NT0_BACLD Putative uncharacterized protein Length = 499 Score = 182 bits (463), Expect = 9e-45, Method: Compositional matrix adjust. Identities = 88/151 (58%), Positives = 115/151 (76%), Gaps = 2/151 (1%) Query: 1 MTLSAYSTYDALGLAALVRAREVSPRELVEAAFARLDEVNPLLNAVVRTRREAALKEADA 60 M +S Y +DA+ L+ L++ EV+ EL++AAF+RL+EVNP LNAV+RTR+E E+ Sbjct: 1 MNISDYVKHDAVALSKLIKHGEVTVNELIDAAFSRLEEVNPELNAVIRTRKEQVKIESKQ 60 Query: 61 MPPDDPSRPFAGVPFLLKDISQAIGGEPLTSGALLLKDNIAKRDSNYVARIRAGGFIPLG 120 + D +PFAGVP LLKDISQA+ GEPLT+GA LL KRDSN+VA++R GF+ LG Sbjct: 61 L--DIRHQPFAGVPILLKDISQALKGEPLTAGAKLLSSYKPKRDSNFVAKLRGAGFLFLG 118 Query: 121 HTNTPEFGLKNITEPLLHGPARNPWSTDCSP 151 TNTPE+GLKNI+EP L+GP+RNPW+TD SP Sbjct: 119 QTNTPEYGLKNISEPELYGPSRNPWNTDYSP 149 >C1PAQ8.1 C1PAQ8_BACCO Amidase Length = 495 Score = 174 bits (441), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 87/146 (59%), Positives = 111/146 (76%), Gaps = 2/146 (1%) Query: 1 MTLSAYSTYDALGLAALVRAREVSPRELVEAAFARLDEVNPLLNAVVRTRREAALKEADA 60 M SAY +YDA GLA LV+ +EVS E++EAA AR+++ N LNAV+RTR E A KEA Sbjct: 1 MEESAYLSYDATGLAGLVKRKEVSVEEVLEAAMARIEKTNRRLNAVIRTRFEKAKKEAAT 60 Query: 61 MPPDDPSRPFAGVPFLLKDISQAIGGEPLTSGALLLKDNIAKRDSNYVARIRAGGFIPLG 120 + D+ +PFAGVP LLKD+SQA+ GE LTSG+ LL+ N A+ D+ + A+++ GFI LG Sbjct: 61 LRTDE--QPFAGVPILLKDLSQAVKGELLTSGSKLLQQNRAREDAFFTAKLKEAGFIILG 118 Query: 121 HTNTPEFGLKNITEPLLHGPARNPWS 146 HTN PEFGLKNITEP L+GPARNPW+ Sbjct: 119 HTNVPEFGLKNITEPELYGPARNPWN 144 >Q8ESC9.1 Q8ESC9_OCEIH 6-aminohexanoate-cyclic-dimer hydrolase Length = 502 Score = 156 bits (395), Expect = 8e-37, Method: Compositional matrix adjust. Identities = 76/151 (50%), Positives = 101/151 (66%), Gaps = 1/151 (0%) Query: 1 MTLSAYSTYDALGLAALVRAREVSPRELVEAAFARLDEVNPLLNAVVRTRREAALKEADA 60 M + Y ++DA+GLA L++ ++V EL+ AF RL+EVN LN + +R E KEA Sbjct: 1 MNFNEYISHDAIGLAKLIKNKQVHANELINLAFNRLNEVNDELNIITHSREERVKKEA-L 59 Query: 61 MPPDDPSRPFAGVPFLLKDISQAIGGEPLTSGALLLKDNIAKRDSNYVARIRAGGFIPLG 120 P+ F GVP L+K+ISQAI GEP++SG+ LLKD K D+ +V + R GF+ +G Sbjct: 60 NEITIPNGMFQGVPILMKNISQAIEGEPMSSGSKLLKDVTYKHDNYFVKKFREAGFLFMG 119 Query: 121 HTNTPEFGLKNITEPLLHGPARNPWSTDCSP 151 HTNTPEFGLKNITEP L+G NPW+T SP Sbjct: 120 HTNTPEFGLKNITEPQLYGATHNPWNTLHSP 150 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.135 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,033,185,392 Number of extensions: 44442411 Number of successful extensions: 97072 Number of sequences better than 1.0e-05: 2432 Number of HSP's gapped: 95581 Number of HSP's successfully gapped: 2432 Length of query: 191 Length of database: 3,060,696,203 Length adjustment: 128 Effective length of query: 63 Effective length of database: 1,854,806,283 Effective search space: 116852795829 Effective search space used: 116852795829 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)