BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0449.0009.p (554 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value O66185.1 O66185_BACCI Mannanase 388 e-106 Q49HI4.1 Q49HI4_BACCI Mannanase EC=3.2.1.78 384 e-104 Q0IJR6.1 Q0IJR6_BACCI Mannanase EC=3.2.1.78 384 e-104 Q4W8M3.1 Q4W8M3_9BACI Mannanase 375 e-102 Q6QHT4.1 Q6QHT4_BACCI Mannanase 369 e-100 >O66185.1 O66185_BACCI Mannanase Length = 516 Score = 388 bits (996), Expect = e-106, Method: Compositional matrix adjust. Identities = 194/322 (60%), Positives = 237/322 (73%), Gaps = 4/322 (1%) Query: 14 ILALTFILILSLSIPQKQAEAAGTSVKGFYVSGNNLFDATGSPFVMRGVNHAHTWYKNDL 73 I + F+L+ S + Q + GFYVSG L DATG PFVMRGVNHAHTWYK+ L Sbjct: 13 IAFVAFLLMFSWT---GQLTNKAHAASGFYVSGTKLLDATGQPFVMRGVNHAHTWYKDQL 69 Query: 74 XXXXXXXXXTGANTVRIVLSNGGQWSLDSLSDVQNILALCDQYKLTAMLEVHDATGSDNA 133 TGANT+RIVL+NG +W+LD ++ V NIL LC+Q KL A+LEVHDATGSD+ Sbjct: 70 STAIPAIAKTGANTIRIVLANGHKWTLDDVNTVNNILTLCEQNKLIAVLEVHDATGSDSL 129 Query: 134 STLNAAVNYWISIKDALIGKEDRVIVNIANEWFGSWSTPAWASGYQSAIPALRAAGIKNT 193 S L+ AVNYWI IK ALIGKEDRVI+NIANEW+G+W AWA+GY+ AIP LR AG+ +T Sbjct: 130 SDLDNAVNYWIGIKSALIGKEDRVIINIANEWYGTWDGVAWANGYKQAIPKLRNAGLTHT 189 Query: 194 LVVDAAGWGQYPSSIFTSGNAVFNSDPLANTIFSIHMYEYSGGTAATVKSNIDNALAIGV 253 L+VD+AGWGQYP S+ G V N+DPL NT+FSIHMYEY+GG A+TVKSNID L + Sbjct: 190 LIVDSAGWGQYPDSVKNYGTEVLNADPLKNTVFSIHMYEYAGGNASTVKSNIDGVLNKNL 249 Query: 254 PVIIGEFGWKHSGGDVDEPAIMSYSQEKGVGWLAWSWYGNGGGVEYLDLASGPAG-TLSD 312 +IIGEFG +H+ GDVDE IMSYSQEKGVGWLAWSW GN + YLD+ + AG +L+ Sbjct: 250 ALIIGEFGGQHTNGDVDEATIMSYSQEKGVGWLAWSWKGNSSDLAYLDMTNDWAGNSLTS 309 Query: 313 WGKSVVNGPYGTKATSALNKIY 334 +G +VVNG G KATS L+ I+ Sbjct: 310 FGNTVVNGSNGIKATSVLSGIF 331 >Q49HI4.1 Q49HI4_BACCI Mannanase EC=3.2.1.78 Length = 327 Score = 384 bits (987), Expect = e-104, Method: Compositional matrix adjust. Identities = 187/294 (63%), Positives = 224/294 (76%) Query: 41 GFYVSGNNLFDATGSPFVMRGVNHAHTWYKNDLXXXXXXXXXTGANTVRIVLSNGGQWSL 100 GFYV+G L+D+TG FVMRGVNH HTWYKNDL TGANTVR+VLSNG QW+ Sbjct: 34 GFYVNGTKLYDSTGKAFVMRGVNHPHTWYKNDLNAAIPAIAQTGANTVRVVLSNGSQWTK 93 Query: 101 DSLSDVQNILALCDQYKLTAMLEVHDATGSDNASTLNAAVNYWISIKDALIGKEDRVIVN 160 D L+ V +I++L Q+++ A+LEVHDATG D ++L AAV+YWISIK ALIGKEDRVIVN Sbjct: 94 DDLNSVNSIISLVSQHQMIAVLEVHDATGKDEYASLEAAVDYWISIKGALIGKEDRVIVN 153 Query: 161 IANEWFGSWSTPAWASGYQSAIPALRAAGIKNTLVVDAAGWGQYPSSIFTSGNAVFNSDP 220 IANEW+G+W++ WA GY+ AIP LR AGIKNTL+VDAAGWGQYP SI G AVF SD Sbjct: 154 IANEWYGNWNSSGWADGYKQAIPKLRNAGIKNTLIVDAAGWGQYPQSIVDEGAAVFASDQ 213 Query: 221 LANTIFSIHMYEYSGGTAATVKSNIDNALAIGVPVIIGEFGWKHSGGDVDEPAIMSYSQE 280 L NT+FSIHMYEY+G AATVK+N+D+ L G+P+IIGEFG H G DVDE AIM Y Q+ Sbjct: 214 LKNTVFSIHMYEYAGKDAATVKTNMDDVLNKGLPLIIGEFGGYHQGADVDEIAIMKYGQQ 273 Query: 281 KGVGWLAWSWYGNGGGVEYLDLASGPAGTLSDWGKSVVNGPYGTKATSALNKIY 334 K VGWLAWSWYGN + LDLA+GP+G L+ WG +VV+G G + TS IY Sbjct: 274 KEVGWLAWSWYGNSPELNDLDLAAGPSGNLTGWGNTVVHGTDGIQQTSKKAGIY 327 >Q0IJR6.1 Q0IJR6_BACCI Mannanase EC=3.2.1.78 Length = 326 Score = 384 bits (985), Expect = e-104, Method: Compositional matrix adjust. Identities = 186/294 (63%), Positives = 226/294 (76%) Query: 41 GFYVSGNNLFDATGSPFVMRGVNHAHTWYKNDLXXXXXXXXXTGANTVRIVLSNGGQWSL 100 GFYV+G L+D+TG PF MRG+NH H+W+KNDL TGANTVRIVLSNG Q++ Sbjct: 33 GFYVNGGKLYDSTGKPFYMRGINHGHSWFKNDLNTAIPAIAKTGANTVRIVLSNGTQYTK 92 Query: 101 DSLSDVQNILALCDQYKLTAMLEVHDATGSDNASTLNAAVNYWISIKDALIGKEDRVIVN 160 D L+ V+NI+ + + K+ A+LEVHDATG D+ ++L+AAVNYWISIK+ALIGKEDRVIVN Sbjct: 93 DDLNSVKNIINVVNANKMIAVLEVHDATGKDDFNSLDAAVNYWISIKEALIGKEDRVIVN 152 Query: 161 IANEWFGSWSTPAWASGYQSAIPALRAAGIKNTLVVDAAGWGQYPSSIFTSGNAVFNSDP 220 IANEW+G+W+ AWA GY+ AIP LR AGIKNTL+VDAAGWGQYP SI G +VF +D Sbjct: 153 IANEWYGTWNGSAWADGYKKAIPKLRDAGIKNTLIVDAAGWGQYPQSIVDYGQSVFAADS 212 Query: 221 LANTIFSIHMYEYSGGTAATVKSNIDNALAIGVPVIIGEFGWKHSGGDVDEPAIMSYSQE 280 NT FSIHMYEY+G AATVKSN++N L G+ +IIGEFG H+ GDVDE AIM Y E Sbjct: 213 QKNTAFSIHMYEYAGKDAATVKSNMENVLNKGLALIIGEFGGYHTNGDVDEYAIMKYGLE 272 Query: 281 KGVGWLAWSWYGNGGGVEYLDLASGPAGTLSDWGKSVVNGPYGTKATSALNKIY 334 KGVGWLAWSWYGN G+ YLDLA+GP G+L+ +G +VVN YG K TS I+ Sbjct: 273 KGVGWLAWSWYGNSSGLNYLDLATGPNGSLTSYGNTVVNDTYGIKNTSQKAGIF 326 >Q4W8M3.1 Q4W8M3_9BACI Mannanase Length = 490 Score = 375 bits (963), Expect = e-102, Method: Compositional matrix adjust. Identities = 176/299 (58%), Positives = 224/299 (74%), Gaps = 1/299 (0%) Query: 41 GFYVSGNNLFDATGSPFVMRGVNHAHTWYKNDLXXXXXXXXXTGANTVRIVLSNGGQWSL 100 GFYVSG L+DA G+PFVMRG+NH H WYK+ TGANTVRIVLS+GGQW+ Sbjct: 35 GFYVSGTTLYDANGNPFVMRGINHGHAWYKDQATTAIEGIANTGANTVRIVLSDGGQWTK 94 Query: 101 DSLSDVQNILALCDQYKLTAMLEVHDATGSDNASTLNAAVNYWISIKDALIGKEDRVIVN 160 D + V+N+++L + L A+LEVHDATG D+ ++LN AV+YWI ++ ALIGKED VI+N Sbjct: 95 DDIQTVRNLISLAEDNNLVAVLEVHDATGYDSIASLNRAVDYWIEMRSALIGKEDTVIIN 154 Query: 161 IANEWFGSWSTPAWASGYQSAIPALRAAGIKNTLVVDAAGWGQYPSSIFTSGNAVFNSDP 220 IANEWFGSW AWA GY+ AIP LR AG+ NTL++DAAGWGQ+P SI G VFN+DP Sbjct: 155 IANEWFGSWDGAAWADGYKQAIPRLRNAGLNNTLMIDAAGWGQFPQSIHDYGREVFNADP 214 Query: 221 LANTIFSIHMYEYSGGTAATVKSNIDNALAIGVPVIIGEFGWKHSGGDVDEPAIMSYSQE 280 NT+FSIHMYEY+GG A+ V++NID L + ++IGEFG +H+ GDVDE IMSYS++ Sbjct: 215 QRNTMFSIHMYEYAGGNASQVRTNIDRVLNQDLALVIGEFGHRHTNGDVDESTIMSYSEQ 274 Query: 281 KGVGWLAWSWYGNGGGVEYLDLASGPAG-TLSDWGKSVVNGPYGTKATSALNKIYTTPG 338 +GVGWLAWSW GNG EYLDL++ AG L+ WG ++VNGPYG + TS L+ ++T G Sbjct: 275 RGVGWLAWSWKGNGPEWEYLDLSNDWAGNNLTAWGNTIVNGPYGLRETSKLSTVFTGGG 333 >Q6QHT4.1 Q6QHT4_BACCI Mannanase Length = 493 Score = 369 bits (946), Expect = e-100, Method: Compositional matrix adjust. Identities = 172/295 (58%), Positives = 222/295 (75%), Gaps = 1/295 (0%) Query: 41 GFYVSGNNLFDATGSPFVMRGVNHAHTWYKNDLXXXXXXXXXTGANTVRIVLSNGGQWSL 100 GFYVSG L+DA G+PFVMRG+NH H WYK+ T ANT+RIVLS+GGQW+ Sbjct: 38 GFYVSGTTLYDANGNPFVMRGINHGHAWYKDQATTAIEGIANTDANTIRIVLSDGGQWTK 97 Query: 101 DSLSDVQNILALCDQYKLTAMLEVHDATGSDNASTLNAAVNYWISIKDALIGKEDRVIVN 160 D + V+N+++L ++ L A+LEVHDATG D+ ++LN AV+YWI ++ ALIGKED VI+N Sbjct: 98 DDIQTVRNLISLAEENNLVAVLEVHDATGYDSIASLNRAVDYWIEMRSALIGKEDTVIIN 157 Query: 161 IANEWFGSWSTPAWASGYQSAIPALRAAGIKNTLVVDAAGWGQYPSSIFTSGNAVFNSDP 220 IANEWFGSW AWA GY+ AIP LR AG+ +TL+VDAAGWGQ+P SI G VFN+DP Sbjct: 158 IANEWFGSWEGAAWADGYKQAIPRLRNAGLNHTLMVDAAGWGQFPQSIHDFGREVFNADP 217 Query: 221 LANTIFSIHMYEYSGGTAATVKSNIDNALAIGVPVIIGEFGWKHSGGDVDEPAIMSYSQE 280 NT+FSIHMYEY+GG A+ V++NID L + ++IGEFG +H+ GDVDE IMSYS++ Sbjct: 218 QRNTMFSIHMYEYAGGNASQVRTNIDRVLNQDLALVIGEFGHRHTNGDVDEATIMSYSEQ 277 Query: 281 KGVGWLAWSWYGNGGGVEYLDLASGPAG-TLSDWGKSVVNGPYGTKATSALNKIY 334 +GVGWLAWSW GNG EYLDL++ AG L+ WG ++VNGPYG + TS L+ ++ Sbjct: 278 RGVGWLAWSWKGNGPEWEYLDLSNDWAGNNLTAWGNTIVNGPYGLRETSRLSTVF 332 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.132 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,122,583,777 Number of extensions: 129711023 Number of successful extensions: 310161 Number of sequences better than 1.0e-05: 231 Number of HSP's gapped: 309647 Number of HSP's successfully gapped: 261 Length of query: 554 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 413 Effective length of database: 1,732,333,088 Effective search space: 715453565344 Effective search space used: 715453565344 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 134 (56.2 bits)