BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0449.0007.p (346 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K5H0.1 B3K5H0_9BACI Periplasmic binding protein 502 e-140 A3I5R9.1 A3I5R9_9BACI Iron (III) dicitrate transport system (Iro... 335 4e-90 B1HRI4.1 B1HRI4_LYSSC Achromobactin-binding periplasmic protein 333 2e-89 Q9K6D8.1 Q9K6D8_BACHD Iron (III) dicitrate transport system (Iro... 331 5e-89 B4AG44.1 B4AG44_BACPU Ferric siderophore ABC superfamily ATP bin... 324 8e-87 >B3K5H0.1 B3K5H0_9BACI Periplasmic binding protein Length = 337 Score = 502 bits (1292), Expect = e-140, Method: Compositional matrix adjust. Identities = 247/340 (72%), Positives = 267/340 (78%), Gaps = 7/340 (2%) Query: 8 WKKTWXXXXXXXXXXXXXSCGQNPGXXXXXXXXXXXXXXXXXXXXXXXXNSTEPASE-EV 66 WKK W CGQN G + PA+E EV Sbjct: 4 WKKPWLFAMLLVIVLVVSGCGQNQGSVSKEPASQQSGSPTIGSQS----EAITPANESEV 59 Query: 67 RTIKHEMGETQITGTPVRIVSLYQGANDAXXXXAYGVKPVGIVESWLEKPVYEYLRADLG 126 RTIKHEMGET+I G P RI++LYQGANDA AYGVKPVGIVESWL+KPVYEYLR +L Sbjct: 60 RTIKHEMGETKIQGNPTRIITLYQGANDAAV--AYGVKPVGIVESWLQKPVYEYLRDELD 117 Query: 127 GIAQIGTEVQPNLEEIYKLKPDVIFANKIRHEAVYDQLSQIAPTVMTGEVYDWKATVKLM 186 + Q+GTEVQPNLEEIYKLKPDVIFA K RHE +YDQLSQIAPTVM GEVYDWKA+V LM Sbjct: 118 DVPQLGTEVQPNLEEIYKLKPDVIFATKTRHEGIYDQLSQIAPTVMIGEVYDWKASVNLM 177 Query: 187 GEVLNQQDKTAELMGEWDSRVADFKQKMGDRLPIEATITNFREDHARIFYMGYAGGILKE 246 GEVLN++DK+ ELM EWDSRV DFKQKMGDRLPIEATITNFR DHARIFYMGYAGGILKE Sbjct: 178 GEVLNREDKSKELMAEWDSRVDDFKQKMGDRLPIEATITNFRADHARIFYMGYAGGILKE 237 Query: 247 LGFTRPEGHDADTWGVQLTSKESIPDMNADVIFNFNSGTDEAAVNKLYTDWTSHPLWKNL 306 LGF RPEGHDA+ WGVQLTSKESIPDMNADVIFNFNSG D AAV K Y DWTSHPLW NL Sbjct: 238 LGFARPEGHDAEVWGVQLTSKESIPDMNADVIFNFNSGDDMAAVEKYYKDWTSHPLWANL 297 Query: 307 DAVKNNQVHMVDEVAWNMAGGILSAHLMLDSLYEMFDLPK 346 DAVKNNQV+MVDEV+WNMAGGILSAHLMLDSLY++F+L K Sbjct: 298 DAVKNNQVYMVDEVSWNMAGGILSAHLMLDSLYDLFELDK 337 >A3I5R9.1 A3I5R9_9BACI Iron (III) dicitrate transport system (Iron (III)-binding protein) Length = 335 Score = 335 bits (858), Expect = 4e-90, Method: Compositional matrix adjust. Identities = 164/285 (57%), Positives = 205/285 (71%), Gaps = 9/285 (3%) Query: 67 RTIKHEMGETQITGTPVRIVSLYQGANDAXXXXAYGVKPVGIVESWLEKPVYEYLRADLG 126 R I H MG T I GTP +IV+LYQGA D A G+KPVG VESW E P Y YL+ DL Sbjct: 55 REITHAMGTTTIEGTPTKIVTLYQGATDVAV--AMGIKPVGAVESWAEAPFYNYLKKDLD 112 Query: 127 GIAQIGTEVQPNLEEIYKLKPDVIFANKIRHEAVYDQLSQIAPTVMTGEVYDWKATVKLM 186 G+A +G E QPNLEEI KLKPD+I A+K+RHE +YDQLSQIAPTV V+D+K T ++M Sbjct: 113 GVAVVGQETQPNLEEIAKLKPDLIIASKVRHEEIYDQLSQIAPTVAHETVFDFKGTAEMM 172 Query: 187 GEVLNQQDKTAELMGEWDSRVADF----KQKMGDRLPIEATITNFREDHARIFYMGYAGG 242 G+ +NQ++K EL GEWD+RVADF K+K+GD+ P ++ NFR DHARI+ GYAG Sbjct: 173 GQAMNQEEKVKELFGEWDTRVADFQTKIKEKLGDKWPFHVSVVNFRADHARIYVTGYAGS 232 Query: 243 ILKELGFTRPEGHDADTWG-VQLTSKESIPDMNADVIFNFNSGTDEAAVNKLYTDWTSHP 301 IL ELGF P+ D+ V+LT KESI MNADVI+ F ++ AV K Y +WT+HP Sbjct: 233 ILSELGFQGPKNLTDDSLEIVKLTDKESITQMNADVIYMFME--NDEAVKKNYEEWTNHP 290 Query: 302 LWKNLDAVKNNQVHMVDEVAWNMAGGILSAHLMLDSLYEMFDLPK 346 LWK LDAVK NQV+ VDE+ WN+AGG +SA+LMLD +Y+ F+L K Sbjct: 291 LWKELDAVKANQVYQVDEINWNLAGGFISANLMLDDIYDKFELDK 335 >B1HRI4.1 B1HRI4_LYSSC Achromobactin-binding periplasmic protein Length = 311 Score = 333 bits (853), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 166/294 (56%), Positives = 209/294 (71%), Gaps = 12/294 (4%) Query: 58 STEPASEEVRTIKHEMGETQITGTPVRIVSLYQGANDAXXXXAYGVKPVGIVESWLEKPV 117 +TE +S R I H MG T I GTP +IV+LYQGA D A G+KPVG VESW E P Sbjct: 25 NTESSS---REITHAMGTTTIEGTPTKIVTLYQGATDVAV--AMGIKPVGAVESWAEAPF 79 Query: 118 YEYLRADLGGIAQIGTEVQPNLEEIYKLKPDVIFANKIRHEAVYDQLSQIAPTVMTGEVY 177 Y YL+ DL G+A +G E QPNLEEI KLKPD+I A+K+RHE +YDQLSQIAPTV V+ Sbjct: 80 YNYLKKDLDGVAVVGQETQPNLEEIAKLKPDLIIASKVRHEEIYDQLSQIAPTVAHETVF 139 Query: 178 DWKATVKLMGEVLNQQDKTAELMGEWDSRVADF----KQKMGDRLPIEATITNFREDHAR 233 D+K T ++MG+ +N+++K EL G+WD+RVADF K+K+GD+ P + NFR DHAR Sbjct: 140 DFKGTAEMMGQAMNEEEKVKELFGDWDTRVADFQTKMKEKLGDKWPFHVAVVNFRADHAR 199 Query: 234 IFYMGYAGGILKELGFTRPEGHDADTWG-VQLTSKESIPDMNADVIFNFNSGTDEAAVNK 292 I+ GYAG IL ELGF P+ D+ V+LT KESI MNADVI+ F D AV K Sbjct: 200 IYVTGYAGSILSELGFQGPKNLTDDSLEIVKLTDKESIAQMNADVIYMFMENDD--AVKK 257 Query: 293 LYTDWTSHPLWKNLDAVKNNQVHMVDEVAWNMAGGILSAHLMLDSLYEMFDLPK 346 Y +WT+HPLWK LDAVK NQV+ VDE+ WN+AGG++SA+LMLD +Y+ F+L K Sbjct: 258 NYEEWTNHPLWKELDAVKANQVYQVDEINWNLAGGLISANLMLDDIYDKFELDK 311 >Q9K6D8.1 Q9K6D8_BACHD Iron (III) dicitrate transport system (Iron (III)-binding protein) Length = 334 Score = 331 bits (849), Expect = 5e-89, Method: Compositional matrix adjust. Identities = 160/285 (56%), Positives = 209/285 (73%), Gaps = 8/285 (2%) Query: 66 VRTIKHEMGETQITGTPVRIVSLYQGANDAXXXXAYGVKPVGIVESWLEKPVYEYLRADL 125 VR + H MG T I G P RIV+LYQGA DA A+G+ PVG+VESWLE P+YEYLR DL Sbjct: 54 VREVSHAMGVTTIEGKPERIVTLYQGATDATV--AFGMTPVGVVESWLEPPMYEYLRDDL 111 Query: 126 GGIAQIGTEVQPNLEEIYKLKPDVIFANKIRHEAVYDQLSQIAPTVMTGEVYDWKATVKL 185 G+ +G E QPNLEEI KL+PD+I A+++RHE VYDQLS+IAPTV+ V+++K TV+L Sbjct: 112 EGVEFVGQETQPNLEEIAKLQPDLIIASQLRHEEVYDQLSEIAPTVVHETVFEFKETVEL 171 Query: 186 MGEVLNQQDKTAELMGEWDSRVADFK----QKMGDRLPIEATITNFREDHARIFYMGYAG 241 MGE +++++K A+++ WD RV DFK +KMGD P+E ++ NFREDHARI+ G++ Sbjct: 172 MGEAMDEEEKAADILAAWDERVEDFKTKVVEKMGDEWPVEVSVLNFREDHARIYATGFSA 231 Query: 242 GILKELGFTRPEGHDADTWGVQLTSKESIPDMNADVIFNFNSGTDEAAVNKLYTDWTSHP 301 IL++LGF PE LT ESIP+MNADV + F S D+ V K Y +WTSHP Sbjct: 232 IILEDLGFEFPENQKNSDEIFMLTDMESIPEMNADVFYMFMS--DDETVQKTYEEWTSHP 289 Query: 302 LWKNLDAVKNNQVHMVDEVAWNMAGGILSAHLMLDSLYEMFDLPK 346 LW+NLDAVK +QV+MVDE+AWNM GGI +A+LMLD +Y+ F+L K Sbjct: 290 LWENLDAVKADQVYMVDEIAWNMGGGINAANLMLDDIYDRFELEK 334 >B4AG44.1 B4AG44_BACPU Ferric siderophore ABC superfamily ATP binding cassette transporter, binding protein Length = 317 Score = 324 bits (830), Expect = 8e-87, Method: Compositional matrix adjust. Identities = 162/297 (54%), Positives = 209/297 (70%), Gaps = 10/297 (3%) Query: 57 NSTEPAS---EEVRTIKHEMGETQITGTPVRIVSLYQGANDAXXXXAYGVKPVGIVESWL 113 NS+E S E+ RT++ +G T+I G+P R+V+LYQGA DA A G+KPVG+VESWL Sbjct: 24 NSSEGKSNSKEKARTVESAIGSTEIKGSPKRVVTLYQGATDAAV--AMGIKPVGVVESWL 81 Query: 114 EKPVYEYLRADLGGIAQIGTEVQPNLEEIYKLKPDVIFANKIRHEAVYDQLSQIAPTVMT 173 E P Y+YLR DL + +G E+QPNLEEI KL PD+I A+K+RHE ++DQL +IAPTV T Sbjct: 82 EAPTYKYLRDDLKDVKIVGQEIQPNLEEIEKLNPDLIIASKVRHEQIFDQLKEIAPTVAT 141 Query: 174 GEVYDWKATVKLMGEVLNQQDKTAELMGEWDSRVADFKQKMGDRL---PIEATITNFRED 230 V+ +K TVKLMGE LNQQDK+ EL+ +WD RVADFK+K + P+ ++ NFR D Sbjct: 142 ETVFTFKDTVKLMGEALNQQDKSKELLTKWDDRVADFKEKAKKDIKNWPMSVSVVNFRAD 201 Query: 231 HARIFYMGYAGGILKELGFTRPEG-HDADTWGVQLTSKESIPDMNADVIFNFNSGTDEAA 289 HARI+ G+AG IL ELGF P+ D + LT KESIP M+ADVI+ F D+ A Sbjct: 202 HARIYQTGFAGSILTELGFEGPKNVKDKKQDIITLTDKESIPQMDADVIYYFMDDQDK-A 260 Query: 290 VNKLYTDWTSHPLWKNLDAVKNNQVHMVDEVAWNMAGGILSAHLMLDSLYEMFDLPK 346 V K Y +WTSHPLWK LDAVK QVH VDE+ WNM GGI++A++MLD + + + K Sbjct: 261 VEKAYKEWTSHPLWKQLDAVKAKQVHKVDEITWNMGGGIIAANMMLDDIDDRLGIDK 317 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.133 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,929,959,220 Number of extensions: 76243957 Number of successful extensions: 169861 Number of sequences better than 1.0e-05: 1637 Number of HSP's gapped: 167690 Number of HSP's successfully gapped: 1638 Length of query: 346 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 210 Effective length of database: 1,779,438,163 Effective search space: 373682014230 Effective search space used: 373682014230 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)