BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0448.0003.p (180 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B5CP50.1 B5CP50_9FIRM Putative uncharacterized protein 134 3e-30 B1C455.1 B1C455_9FIRM Putative uncharacterized protein 118 2e-25 Q65QE2.1 Q65QE2_MANSM AcrR protein 86 1e-15 A6VKF9.1 A6VKF9_ACTSZ Transcriptional regulator, TetR family 82 2e-14 A8MG83.1 A8MG83_ALKOO Transcriptional regulator, TetR family 75 3e-12 >B5CP50.1 B5CP50_9FIRM Putative uncharacterized protein Length = 188 Score = 134 bits (338), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 71/171 (41%), Positives = 107/171 (62%), Gaps = 5/171 (2%) Query: 1 MEQNLQIVSPMSNQGRNAYVTERLTNTLLVLLRDKPISDISISELCEGAGVGRVSFYRNY 60 M Q + VSPMSN+GRNAYV E + LL LL++K +++ISISE+CE AGVGR+SFYRNY Sbjct: 9 MIQKSKSVSPMSNEGRNAYVIEHINEALLGLLKEKSLNEISISEICETAGVGRMSFYRNY 68 Query: 61 TEKEDILKAYIDLLFQSFMDEYHNYSEGIERPLSDLIRALFTHIEDHLDFYRLLNERGLV 120 KED++K + L Q + +Y EG P + +L H H DFY LL +GL Sbjct: 69 ESKEDVIKKQLLQLIQEWEKDY----EGKNDP-TYFSESLLRHYYKHKDFYLLLYNQGLS 123 Query: 121 YLLKDVVVEICGLKPESSKIEAYSKAFVAYTLYGWVEVWFQRGMKESATEM 171 ++ + + L+ ++ +E Y+K+ +A ++GWV+ W ++GM E+ E+ Sbjct: 124 NMILEALRVSVKLEEANNNLERYAKSMIAGMIWGWVDEWMRQGMPETPEEI 174 >B1C455.1 B1C455_9FIRM Putative uncharacterized protein Length = 168 Score = 118 bits (296), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 68/165 (41%), Positives = 99/165 (60%), Gaps = 5/165 (3%) Query: 11 MSNQGRNAYVTERLTNTLLVLLRDKPISDISISELCEGAGVGRVSFYRNYTEKEDILKAY 70 M N +N YV +T LL LL++K +++ISI E+ A VGRVSFYRNY KEDIL+ Y Sbjct: 1 MDNLKKNQYVKSEITKALLELLKEKDLNEISIREITTRAQVGRVSFYRNYKRKEDILEQY 60 Query: 71 IDLLFQSFMDEYHNYSEGIERPLSDLIRALFTHIEDHLDFYRLLNERGLVYLLKDVVVEI 130 + + + + + S + L++ LF H+ + DFY LL +GL+YL KD + + Sbjct: 61 LFFIIKEWGE-----SNTDSLSMDKLLKKLFEHLVSYRDFYTLLYTKGLLYLFKDTLKRL 115 Query: 131 CGLKPESSKIEAYSKAFVAYTLYGWVEVWFQRGMKESATEMADMF 175 E + AYS AFV+Y +YGW+E W RGM+ESA E+ ++ Sbjct: 116 ITKDQELPNVAAYSVAFVSYGIYGWIEEWIARGMQESADEIYNIL 160 >Q65QE2.1 Q65QE2_MANSM AcrR protein Length = 170 Score = 85.9 bits (211), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 50/162 (30%), Positives = 84/162 (51%), Gaps = 4/162 (2%) Query: 17 NAYVTERLTNTLLVLLRDKPISDISISELCEGAGVGRVSFYRNYTEKEDILKAYIDLLFQ 76 N V E +T LL L+ K +I+I+ + E AGV R+SFYRN+ KED+L Y+ + + Sbjct: 6 NFVVKESITEALLRLMAKKNFDEINITAITELAGVSRISFYRNFDSKEDVLIKYMYVRAK 65 Query: 77 SFMDEYHNYSEGIERPLSDLIRALFTHIEDHLDFYRLLNERGLVYLLKDVVVEICGLKPE 136 E + E + + D + +F IE D LL + L ++ + G KPE Sbjct: 66 ----ELYKPFESQDVSVRDKLIGMFKSIEGMEDIINLLYAQNLSHIFLQYFNFVRGAKPE 121 Query: 137 SSKIEAYSKAFVAYTLYGWVEVWFQRGMKESATEMADMFKNL 178 ++AY + V +G ++ W +RG +E+ +M D+ +N+ Sbjct: 122 QENLDAYQNSIVVGVCFGALDEWIKRGRQETPEQMVDLLQNV 163 >A6VKF9.1 A6VKF9_ACTSZ Transcriptional regulator, TetR family Length = 168 Score = 82.4 bits (202), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 52/161 (32%), Positives = 82/161 (50%), Gaps = 6/161 (3%) Query: 17 NAYVTERLTNTLLVLLRDKPISDISISELCEGAGVGRVSFYRNYTEKEDILKAYIDLLFQ 76 N V E +T L+ L++ K +ISI+E+ AGV R+SFYRN+ KED+L I L++ Sbjct: 6 NFVVKESITEALIRLMKKKSFDNISITEITALAGVSRISFYRNFESKEDVL---IKHLYE 62 Query: 77 SFMDEYHNYSEGIERPLSDLIRALFTHIEDHLDFYRLLNERGLVYLLKDVVVEICGLKPE 136 M + R + + LF+ ++ D LL +GL +L + G KPE Sbjct: 63 RAMTSFTALHADTVR---ERLLGLFSVTDELGDVLDLLYSQGLSHLFLRYFAFVIGAKPE 119 Query: 137 SSKIEAYSKAFVAYTLYGWVEVWFQRGMKESATEMADMFKN 177 S +A+ A + +G ++ W +RG +ES M D+ N Sbjct: 120 QSNEQAFQSAIMMGICFGSLDEWIKRGRQESPEVMVDILLN 160 >A8MG83.1 A8MG83_ALKOO Transcriptional regulator, TetR family Length = 195 Score = 74.7 bits (182), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 48/172 (27%), Positives = 89/172 (51%), Gaps = 16/172 (9%) Query: 16 RNAYVTERLTNTLLVLLRDKPISDISISELCEGAGVGRVSFYRNYTEKEDILKAYIDLLF 75 + Y L N+LL L+ KPI+ I+I ELCE A + R +FY +Y ++ D+L+ D F Sbjct: 9 KTRYTRMALQNSLLELMEKKPIAKITIKELCENADINRTTFYAHYADQYDLLQKIEDETF 68 Query: 76 QSFMDEYHNYSEGIERPLS-DLIRALFTH-IEDHLDFYRLLNERGLVYLLKDVVVEI--- 130 + N + ++ S +++ +F H +E+ L++E+G + K+++ I Sbjct: 69 SWVKESLTNLIDKADQYESMEILEGIFQHFVENRNHLQILMSEKGNINFQKELISLIYQQ 128 Query: 131 CGLKPESSKIEAYSK--------AFVAYTLYGWVEVWFQRGMKESATEMADM 174 C + P ++ AY+ FV G ++ WF+ G+ +SA EM+++ Sbjct: 129 CMISPSTT---AYTNMNFLEDYFVFVVSGSIGLIQHWFKSGLNKSAKEMSEI 177 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.138 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,026,091,149 Number of extensions: 40742237 Number of successful extensions: 117636 Number of sequences better than 1.0e-05: 178 Number of HSP's gapped: 117747 Number of HSP's successfully gapped: 178 Length of query: 180 Length of database: 3,060,696,203 Length adjustment: 127 Effective length of query: 53 Effective length of database: 1,864,227,298 Effective search space: 98804046794 Effective search space used: 98804046794 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)