BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0447.0011.p (166 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value O34663.1 YBCL_BACSU Uncharacterized MFS-type transporter ybcL 228 2e-58 Q65P40.1 Q65P40_BACLD YbcL 223 5e-57 C0ZIQ5.1 C0ZIQ5_BREBN Putative transporter 216 4e-55 B3KC79.1 B3KC79_9BACI Major facilitator superfamily MFS_1 214 3e-54 C1PFN3.1 C1PFN3_BACCO Major facilitator superfamily MFS_1 210 4e-53 >O34663.1 YBCL_BACSU Uncharacterized MFS-type transporter ybcL Length = 390 Score = 228 bits (580), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 115/155 (74%), Positives = 136/155 (87%), Gaps = 2/155 (1%) Query: 1 MSVSWKVYILAIISFLVGTSEYIVSGILDRISLSFGVSLXXXXGGQLITIFSLVYAICTP 60 MS +WK+YILAI+SFLVGTSEYI+SGILD+I+ + G++L GQLITIFSLVYA+ TP Sbjct: 1 MSNTWKIYILAIVSFLVGTSEYIISGILDQIAHTLGITLAA--AGQLITIFSLVYALSTP 58 Query: 61 ILMALTASMDRRRLILSGLGLFVAANILSFVLPGYGLFVVARIIMALGAGIVVVTALNIA 120 +LMALTASMDRR+L++ LGLFV N+L+FVLPGYG F+ ARIIMA+GAG+VVVTAL IA Sbjct: 59 VLMALTASMDRRKLMMYALGLFVFGNVLAFVLPGYGWFIAARIIMAMGAGVVVVTALTIA 118 Query: 121 AKIAREGRQASSIATVVMGFTASLIIGVPLGRMTA 155 AKIA EG+Q S+IATVVMGFTASLIIGVPLGRM A Sbjct: 119 AKIASEGKQGSAIATVVMGFTASLIIGVPLGRMIA 153 >Q65P40.1 Q65P40_BACLD YbcL Length = 390 Score = 223 bits (568), Expect = 5e-57, Method: Compositional matrix adjust. Identities = 114/155 (73%), Positives = 134/155 (86%), Gaps = 2/155 (1%) Query: 1 MSVSWKVYILAIISFLVGTSEYIVSGILDRISLSFGVSLXXXXGGQLITIFSLVYAICTP 60 M +WK+Y+LAIISFLVGTSEYI+SGILD+I+ + G++L GQLITIFSLVYA+ TP Sbjct: 1 MFSTWKIYLLAIISFLVGTSEYIISGILDQIAHTLGITLAA--AGQLITIFSLVYAVFTP 58 Query: 61 ILMALTASMDRRRLILSGLGLFVAANILSFVLPGYGLFVVARIIMALGAGIVVVTALNIA 120 +LMALTASMDRR+L++ LGLFV NIL+F LPGYG FV AR++MALGAG+VVVTAL IA Sbjct: 59 VLMALTASMDRRKLMMYALGLFVIGNILAFTLPGYGWFVAARVVMALGAGMVVVTALTIA 118 Query: 121 AKIAREGRQASSIATVVMGFTASLIIGVPLGRMTA 155 AKIA G+Q S+IATVVMGFTASLIIGVPLGRMTA Sbjct: 119 AKIAPAGKQGSAIATVVMGFTASLIIGVPLGRMTA 153 >C0ZIQ5.1 C0ZIQ5_BREBN Putative transporter Length = 401 Score = 216 bits (551), Expect = 4e-55, Method: Compositional matrix adjust. Identities = 107/155 (69%), Positives = 136/155 (87%), Gaps = 2/155 (1%) Query: 1 MSVSWKVYILAIISFLVGTSEYIVSGILDRISLSFGVSLXXXXGGQLITIFSLVYAICTP 60 MS +WKVY+L+++SFLVGTSEY++SGILD+IS + G+S+ GQL+TIFS VYAI TP Sbjct: 1 MSNTWKVYLLSLVSFLVGTSEYVISGILDKISDTMGISITA--AGQLVTIFSFVYAILTP 58 Query: 61 ILMALTASMDRRRLILSGLGLFVAANILSFVLPGYGLFVVARIIMALGAGIVVVTALNIA 120 ++MA+TA MDRRRL++S LG+FV ANILSF L G+ F++AR+IMA+GAG+VVVTAL+IA Sbjct: 59 VMMAITAKMDRRRLLVSALGIFVVANILSFALQGFEFFIMARVIMAIGAGMVVVTALDIA 118 Query: 121 AKIAREGRQASSIATVVMGFTASLIIGVPLGRMTA 155 AKI+ EG+QASSIATV+MGFTASLIIGVPLGR+ A Sbjct: 119 AKISPEGKQASSIATVIMGFTASLIIGVPLGRVAA 153 >B3KC79.1 B3KC79_9BACI Major facilitator superfamily MFS_1 Length = 394 Score = 214 bits (544), Expect = 3e-54, Method: Compositional matrix adjust. Identities = 109/155 (70%), Positives = 135/155 (87%), Gaps = 2/155 (1%) Query: 1 MSVSWKVYILAIISFLVGTSEYIVSGILDRISLSFGVSLXXXXGGQLITIFSLVYAICTP 60 M+ SWK+Y+LAI+SFLVGTSEYI+SG+LD+I+ S G+++ GQLITIFSLVYAI TP Sbjct: 1 MTGSWKIYVLAIVSFLVGTSEYIISGVLDKIADSLGITITA--AGQLITIFSLVYAILTP 58 Query: 61 ILMALTASMDRRRLILSGLGLFVAANILSFVLPGYGLFVVARIIMALGAGIVVVTALNIA 120 +LMALTA ++RR+L++ LG+FV AN+LSF LPGY FV+AR++MA+GAG+VVVTAL IA Sbjct: 59 VLMALTAKVERRKLLVYSLGVFVVANLLSFALPGYIPFVIARVLMAMGAGMVVVTALGIA 118 Query: 121 AKIAREGRQASSIATVVMGFTASLIIGVPLGRMTA 155 AKIA G+QASSIATVVMGFTASLIIGVPLGR+ A Sbjct: 119 AKIAPPGKQASSIATVVMGFTASLIIGVPLGRVAA 153 >C1PFN3.1 C1PFN3_BACCO Major facilitator superfamily MFS_1 Length = 394 Score = 210 bits (534), Expect = 4e-53, Method: Compositional matrix adjust. Identities = 108/155 (69%), Positives = 133/155 (85%), Gaps = 2/155 (1%) Query: 1 MSVSWKVYILAIISFLVGTSEYIVSGILDRISLSFGVSLXXXXGGQLITIFSLVYAICTP 60 MS + K+YILAI+SFLVGTS+YI+SGILD IS S G++L GQLITIFSLVYAI TP Sbjct: 1 MSNTGKIYILAIVSFLVGTSQYIISGILDNISESLGITLAA--AGQLITIFSLVYAIFTP 58 Query: 61 ILMALTASMDRRRLILSGLGLFVAANILSFVLPGYGLFVVARIIMALGAGIVVVTALNIA 120 +LMA+T+ MD+R+L++ LGLFV NIL+F+LPGYG F+V+RIIMA GAG+VVVTAL IA Sbjct: 59 VLMAITSRMDKRKLMIYSLGLFVIGNILAFILPGYGFFIVSRIIMAFGAGMVVVTALTIA 118 Query: 121 AKIAREGRQASSIATVVMGFTASLIIGVPLGRMTA 155 KIA +G++AS+IATV+MG TASLIIGVPLGRMT+ Sbjct: 119 EKIAPKGKKASAIATVIMGLTASLIIGVPLGRMTS 153 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.329 0.142 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 806,627,878 Number of extensions: 27602445 Number of successful extensions: 161838 Number of sequences better than 1.0e-05: 1847 Number of HSP's gapped: 161022 Number of HSP's successfully gapped: 1847 Length of query: 166 Length of database: 3,060,696,203 Length adjustment: 125 Effective length of query: 41 Effective length of database: 1,883,069,328 Effective search space: 77205842448 Effective search space used: 77205842448 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 126 (53.1 bits)