BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0447.0010.p (224 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C1PFN3.1 C1PFN3_BACCO Major facilitator superfamily MFS_1 220 8e-56 O34663.1 YBCL_BACSU Uncharacterized MFS-type transporter ybcL 218 3e-55 C0ZIQ5.1 C0ZIQ5_BREBN Putative transporter 209 1e-52 A7Z5E1.1 A7Z5E1_BACA2 YbcL 206 1e-51 B3KC79.1 B3KC79_9BACI Major facilitator superfamily MFS_1 204 3e-51 >C1PFN3.1 C1PFN3_BACCO Major facilitator superfamily MFS_1 Length = 394 Score = 220 bits (560), Expect = 8e-56, Method: Compositional matrix adjust. Identities = 113/219 (51%), Positives = 145/219 (66%) Query: 1 LGLISMLIIFLTIPRIKGDEPIPLSQQLALLKEPKVALGLAVTFFWLGGYSIAYTYLSPY 60 LGL++M+II LTIP+I+ ++ PL+QQLALLK+PKV LGLA+TFFWLGGYSI YTYLSPY Sbjct: 168 LGLVAMVIISLTIPKIQVEKLTPLNQQLALLKKPKVVLGLAITFFWLGGYSIVYTYLSPY 227 Query: 61 LITISGINDSYISGALLIFGIASMIGSKIGGFSADRWGVYPTLFGXXXXXXXXXXXXXXX 120 L+ ISG++D +SG LLIFGIAS++GSK GGFS DRWGV TL G Sbjct: 228 LLNISGVSDKLLSGVLLIFGIASLVGSKFGGFSTDRWGVRFTLIGGMILHITMLVLLSIV 287 Query: 121 XXXXXXXXAVLLLWSFAAWSTGPTQQYHITTLKPEASGVMLGLNQSXXXXXXXXXXXXXX 180 +L+LWSFAAWSTGPTQQYH+ T++PE+S V+LGLNQS Sbjct: 288 THSYVGVLVILILWSFAAWSTGPTQQYHLATIEPESSSVLLGLNQSMMQLAMAAGAGIGG 347 Query: 181 XXXXXXXLSSITWIGAASVFLATSATLILYRLGAERKTV 219 L+SITW+ A V +A T++ R +++ +V Sbjct: 348 IFVERISLTSITWLAALGVTIAIGLTILTSRSPSKKTSV 386 >O34663.1 YBCL_BACSU Uncharacterized MFS-type transporter ybcL Length = 390 Score = 218 bits (555), Expect = 3e-55, Method: Compositional matrix adjust. Identities = 108/211 (51%), Positives = 141/211 (66%) Query: 1 LGLISMLIIFLTIPRIKGDEPIPLSQQLALLKEPKVALGLAVTFFWLGGYSIAYTYLSPY 60 LGLI+M++IF T+P +GD+P+PL QQLAL K+ KVA+GL++TFFWLGGYS+AYTYLSPY Sbjct: 168 LGLIAMVVIFFTLPYTEGDKPVPLLQQLALFKKRKVAMGLSITFFWLGGYSVAYTYLSPY 227 Query: 61 LITISGINDSYISGALLIFGIASMIGSKIGGFSADRWGVYPTLFGXXXXXXXXXXXXXXX 120 L+ ISGIN +SG LLIFGIAS++GSK GG+S D+WGV TL G Sbjct: 228 LLNISGINGKLLSGVLLIFGIASLVGSKFGGYSTDKWGVPFTLVGGMTLHIVTLILLSLV 287 Query: 121 XXXXXXXXAVLLLWSFAAWSTGPTQQYHITTLKPEASGVMLGLNQSXXXXXXXXXXXXXX 180 +L+LWSFAAWSTGPTQQ+H+ T++PE SGV+L +NQS Sbjct: 288 THSYIGVLVILILWSFAAWSTGPTQQFHLATIEPEMSGVLLSMNQSMMQFAMAVGAGIGG 347 Query: 181 XXXXXXXLSSITWIGAASVFLATSATLILYR 211 L+SITW+GA V +A A+L+++ Sbjct: 348 VFVENVSLASITWVGALGVMIAIIASLLIFN 378 >C0ZIQ5.1 C0ZIQ5_BREBN Putative transporter Length = 401 Score = 209 bits (533), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 109/218 (50%), Positives = 141/218 (64%), Gaps = 3/218 (1%) Query: 2 GLISMLIIFLTIPRIKGDEPIPLSQQLALLKEPKVALGLAVTFFWLGGYSIAYTYLSPYL 61 GL++M+++ TIP++KGD P+PLS+QLALLK PKV LGL+VTFFWLGGYSIAYTY+SPYL Sbjct: 169 GLLAMVVLSRTIPQVKGDVPVPLSKQLALLKNPKVGLGLSVTFFWLGGYSIAYTYISPYL 228 Query: 62 ITISGINDSYISGALLIFGIASMIGSKIGGFSADRWGVYPTLFGXXXXXXXXXXXXXXXX 121 + + I+++ +S ALL FG+AS+IGSK GG+S D+WGV PTL G Sbjct: 229 LEAADISENLLSSALLAFGLASLIGSKFGGYSTDKWGVSPTLLGGMLLHVAALILLSITV 288 Query: 122 XXXX---XXXAVLLLWSFAAWSTGPTQQYHITTLKPEASGVMLGLNQSXXXXXXXXXXXX 178 A+L+LWSFAAWS+GPTQQ+++ + P SGVMLGLNQS Sbjct: 289 SISHEPFTVIAILVLWSFAAWSSGPTQQFNLVRIAPNNSGVMLGLNQSMMQLAMAAGAGI 348 Query: 179 XXXXXXXXXLSSITWIGAASVFLATSATLILYRLGAER 216 LSSITW+G + V +A A LIL + ER Sbjct: 349 GGIAVDQVSLSSITWLGMSGVAIAIVAALILQVMLKER 386 >A7Z5E1.1 A7Z5E1_BACA2 YbcL Length = 391 Score = 206 bits (524), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 106/213 (49%), Positives = 133/213 (62%) Query: 6 MLIIFLTIPRIKGDEPIPLSQQLALLKEPKVALGLAVTFFWLGGYSIAYTYLSPYLITIS 65 MLII +P IKGD+P+PL QQ +LLK+ KVA GLA+TFFWLGGYS+AYTYLSPYL+ ++ Sbjct: 173 MLIIRFALPEIKGDKPVPLIQQFSLLKKRKVAAGLAITFFWLGGYSVAYTYLSPYLLNVT 232 Query: 66 GINDSYISGALLIFGIASMIGSKIGGFSADRWGVYPTLFGXXXXXXXXXXXXXXXXXXXX 125 I +S LLIFG+AS+ GSK GGFSAD+WG TL G Sbjct: 233 HIGSGILSTLLLIFGVASLAGSKAGGFSADKWGARMTLTGGMALHAIMLMLLPFVTHSAF 292 Query: 126 XXXAVLLLWSFAAWSTGPTQQYHITTLKPEASGVMLGLNQSXXXXXXXXXXXXXXXXXXX 185 AVL+ WSFAAWS+GP QQYH+ T++PE+SGV+LGLNQS Sbjct: 293 GAAAVLIFWSFAAWSSGPAQQYHLATMEPESSGVLLGLNQSVMQLAMAAGAGIGGIVVTH 352 Query: 186 XXLSSITWIGAASVFLATSATLILYRLGAERKT 218 L+S+TWIGAA V +A L L L +E+ + Sbjct: 353 LSLASVTWIGAAGVVIAMCGVLSLASLTSEKTS 385 >B3KC79.1 B3KC79_9BACI Major facilitator superfamily MFS_1 Length = 394 Score = 204 bits (520), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 114/220 (51%), Positives = 141/220 (64%), Gaps = 2/220 (0%) Query: 1 LGLISMLIIFLTIPRIKGDEPIPLSQQLALLKEPKVALGLAVTFFWLGGYSIAYTYLSPY 60 LG+ISML++ IP+ GD P+PL QQ ALLK+PKVALGLA+TFFWLGGYS+AYTYLSPY Sbjct: 168 LGVISMLVLHKAIPKTTGDAPVPLLQQFALLKKPKVALGLAITFFWLGGYSVAYTYLSPY 227 Query: 61 LITISGINDSYISGALLIFGIASMIGSKIGGFSADRWGVYPTLFGXXXXXXXXXXXXXXX 120 L+ +SG+N+ +IS AL FGIAS+IGSK GGFS DRWGV TL G Sbjct: 228 LLNVSGLNEQWISSALFAFGIASLIGSKFGGFSTDRWGVPLTLIGGMLLHIIALIVLTFT 287 Query: 121 X--XXXXXXXAVLLLWSFAAWSTGPTQQYHITTLKPEASGVMLGLNQSXXXXXXXXXXXX 178 AVL++WS AAWS+GPTQQY++ L+P +SGVMLGLNQS Sbjct: 288 DAGASVMLVFAVLVIWSLAAWSSGPTQQYNLVQLEPNSSGVMLGLNQSMMQLSMAAGAGI 347 Query: 179 XXXXXXXXXLSSITWIGAASVFLATSATLILYRLGAERKT 218 LSSI WIGAA V +A +L+L++ + T Sbjct: 348 GGIAIERISLSSIAWIGAAGVAIAAVVSLVLFQYIKQEST 387 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.139 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,016,560,218 Number of extensions: 31377135 Number of successful extensions: 93651 Number of sequences better than 1.0e-05: 671 Number of HSP's gapped: 93571 Number of HSP's successfully gapped: 671 Length of query: 224 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 93 Effective length of database: 1,826,543,238 Effective search space: 169868521134 Effective search space used: 169868521134 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)