BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0447.0007.p (153 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q65J92.1 Q65J92_BACLD Putative uncharacterized protein 111 2e-23 Q5WHL0.1 Q5WHL0_BACSK Putative uncharacterized protein 111 2e-23 >Q65J92.1 Q65J92_BACLD Putative uncharacterized protein Length = 207 Score = 111 bits (278), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 56/140 (40%), Positives = 85/140 (60%), Gaps = 1/140 (0%) Query: 4 IGQGYRSHSGTVEDMVTASELLLTSLFRFAGSNRYFME-LLYAGAGNDEVIRSAVNETRN 62 I QGYR +DM+ ++ L+ +F FA NR M LL G G D IR A+ E Sbjct: 68 IHQGYRQTFALADDMIQSTTALMKRIFTFAQENRNLMTFLLVKGQGADPEIREAIEEALI 127 Query: 63 EMERAFRRNIERAVELQMLPANIDSSLRAMLLVSLIEGVISRWLFGPREAESELSRKTAE 122 +E+AF++NI+RA EL ML ++ ++ L+A +L SL+ G +S+WLFGP+ + + + + + Sbjct: 128 SVEQAFKKNIQRATELGMLNSSHNTDLQATMLTSLVLGTLSKWLFGPKHDLNYVPKFSVD 187 Query: 123 ELAAETARFEFFGLLGTTKN 142 E+ E FEFFGL+G N Sbjct: 188 EMTEELVHFEFFGLIGQRGN 207 >Q5WHL0.1 Q5WHL0_BACSK Putative uncharacterized protein Length = 203 Score = 111 bits (277), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 65/142 (45%), Positives = 82/142 (57%), Gaps = 4/142 (2%) Query: 1 VKVIGQGYRSHSGTVEDMVTASELLLTSLFRFAGSNRYFMELLY-AGAGNDEVIRSAVNE 59 + VI GYR G V+DMV +S L+T LF FA +N +M LL+ G +E I ++ Sbjct: 65 LNVIRSGYRQTEGNVDDMVESSRRLMTDLFSFAQANPDYMRLLFKTGQDAEEKICRHISH 124 Query: 60 TRNEMERAFRRNIERAVELQMLPANIDSSLRAMLLVSLIEGVISRWLFGPREAESELSRK 119 T +E AF RNI+RA EL+MLP + S A +LVSL+ G + RWLF L Sbjct: 125 TLIAIEEAFERNIKRAQELEMLPKDSSSKRLAAMLVSLVLGTLERWLFSE---SPHLDNI 181 Query: 120 TAEELAAETARFEFFGLLGTTK 141 AE LA E FEFFGLLG TK Sbjct: 182 AAESLAVEIVSFEFFGLLGKTK 203 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.132 0.355 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 777,080,370 Number of extensions: 25924270 Number of successful extensions: 75272 Number of sequences better than 1.0e-05: 2 Number of HSP's gapped: 75270 Number of HSP's successfully gapped: 2 Length of query: 153 Length of database: 3,060,696,203 Length adjustment: 115 Effective length of query: 38 Effective length of database: 1,977,279,478 Effective search space: 75136620164 Effective search space used: 75136620164 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 125 (52.8 bits)