BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0447.0006.p (93 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q65J91.1 Q65J91_BACLD Putative uncharacterized protein 70 6e-11 B3K3R5.1 B3K3R5_9BACI OsmC family protein 54 6e-06 >Q65J91.1 Q65J91_BACLD Putative uncharacterized protein Length = 128 Score = 70.1 bits (170), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 39/86 (45%), Positives = 53/86 (61%), Gaps = 2/86 (2%) Query: 1 MDILCESLGMCIAVSLVRLMGDAGLD--SNLLTVEVVPHKAHGGAPRVENMNVSVIFPGA 58 +++L SLGMCI +SL ++ G++ + +V V KA G RVE +VSV P Sbjct: 35 IELLEASLGMCITISLQKMFERDGIEVLEDEFSVSVSAAKAPEGPSRVEKCSVSVKLPEH 94 Query: 59 LSTEIKEKLLLQASRVCTIGNTLKRG 84 L T+ K+KL+ A R CTIGNTLKRG Sbjct: 95 LPTDYKKKLITSAERACTIGNTLKRG 120 >B3K3R5.1 B3K3R5_9BACI OsmC family protein Length = 139 Score = 53.5 bits (127), Expect = 6e-06, Method: Compositional matrix adjust. Identities = 31/83 (37%), Positives = 46/83 (55%), Gaps = 2/83 (2%) Query: 2 DILCESLGMCIAVSLVRLMGDAGL--DSNLLTVEVVPHKAHGGAPRVENMNVSVIFPGAL 59 ++L SL +C+++SL ++M G+ D + + +EV KA + + + NV V P L Sbjct: 37 ELLEASLALCVSISLQKIMAYDGVEYDKSSIHIEVSSVKAEDKSNKFSHFNVLVSLPPTL 96 Query: 60 STEIKEKLLLQASRVCTIGNTLK 82 KEKL R CTIGNTLK Sbjct: 97 EPAYKEKLKSVVERACTIGNTLK 119 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 512,127,053 Number of extensions: 16904653 Number of successful extensions: 36494 Number of sequences better than 1.0e-05: 2 Number of HSP's gapped: 36496 Number of HSP's successfully gapped: 2 Length of query: 93 Length of database: 3,060,696,203 Length adjustment: 62 Effective length of query: 31 Effective length of database: 2,476,593,273 Effective search space: 76774391463 Effective search space used: 76774391463 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)