BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0447.0005.p (450 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q65J90.1 Q65J90_BACLD Putative uncharacterized protein 507 e-141 B2A881.1 B2A881_NATTJ Permease for cytosine/purines uracil thiam... 190 3e-46 Q9KBP3.1 Q9KBP3_BACHD Cytosine permease 190 3e-46 C0BX33.1 C0BX33_9CLOT Putative uncharacterized protein 181 1e-43 B0K7W7.1 B0K7W7_THEP3 Permease for cytosine/purines, uracil, thi... 181 2e-43 >Q65J90.1 Q65J90_BACLD Putative uncharacterized protein Length = 449 Score = 507 bits (1305), Expect = e-141, Method: Compositional matrix adjust. Identities = 258/436 (59%), Positives = 317/436 (72%), Gaps = 4/436 (0%) Query: 19 EDYAIQSVPAEKRKSWLNVAFTSCGWIISLSTIFVGGSLAAGMTFGQAILAAVIGMTILA 78 +D+A+ SVP KRKS N+ TSC WIISLSTI GG+L G+ F A+LAAV+GM ILA Sbjct: 14 DDFAVTSVPENKRKSIFNITITSCAWIISLSTIVTGGALIQGLNFAHAVLAAVLGMLILA 73 Query: 79 VYGFFQGWMGAKYGVATAVLARQAFGRAGAGLFGILLSLTMGIGWFGWQVAFFGSTIEQM 138 VYGFFQG MGAKYG++T VLAR AFGR G+ LFGI+L+LT+GIGWFGWQVAFFG+TI +M Sbjct: 74 VYGFFQGVMGAKYGISTTVLARLAFGRYGSSLFGIVLALTLGIGWFGWQVAFFGTTISEM 133 Query: 139 FPGQWFADPTFAIIWGGVLMIFTALIGYKGITYLSFVAVPLVIVLSIWGIFVAADASGGL 198 FPG W A P AI+WGG+LMI TA IGY+G+ LSF+AVPLV++ SIWGI A + SG Sbjct: 134 FPGVWLAKPEVAIVWGGILMILTAFIGYRGLAALSFIAVPLVVIFSIWGIISAVNYSGSW 193 Query: 199 SNLFALQPTGASLGLFAGITLVVGNAALGAIVFPDVSRYGKTPLAGGMGAAVGYFVGGIF 258 NL PTG L +FAGIT+VVGNAALGA+VFPDV+RY K ++GG+GA+VGYF+GG+F Sbjct: 194 HNLLTFHPTGDPLTIFAGITIVVGNAALGAVVFPDVTRYAKGAVSGGIGASVGYFLGGVF 253 Query: 259 SVVAGAAMTFAAQLPDLGATPNIPAVMAKLGMGFFAFLILVFAQWSNNDNNLYTGALGLR 318 +VAGAAM AA +PD+G+TPNIPA M+KLG+GF AFLI+VFAQW+ NDNNLYTG+LGLR Sbjct: 254 CLVAGAAMAIAANVPDIGSTPNIPAAMSKLGLGFLAFLIIVFAQWTTNDNNLYTGSLGLR 313 Query: 319 NVVKLPKFXXXXXXXXXXXXXXXTGYQDHFVPFLNFLGVYVPPIAGVMIADHWI----VR 374 NVV +PK G Q+ FVPFL FLG+YVPPIAGVMIADHWI ++ Sbjct: 314 NVVNIPKKALVAIMGVIGIIIALLGVQNMFVPFLTFLGLYVPPIAGVMIADHWITAPVIK 373 Query: 375 RGTYQFGEGTKYSXXXXXXXXXXXXXXXXXXXXXWGISPINSTVLGLIIYPVLTALLRLL 434 + YQFG GT YS +GI PINST+L +IY + T + L Sbjct: 374 KKQYQFGAGTTYSKLNVAAILSVIIGGFTASKITFGIGPINSTILAFLIYIIFTFIFTKL 433 Query: 435 RIPYEWGQTTEDSTGY 450 + YE G+ TE+S+G+ Sbjct: 434 NLRYEIGEATEESSGF 449 >B2A881.1 B2A881_NATTJ Permease for cytosine/purines uracil thiamine allantoin Length = 455 Score = 190 bits (482), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 143/462 (30%), Positives = 217/462 (46%), Gaps = 28/462 (6%) Query: 9 AGRQGNIAALEDYAIQSVPAEKRKSWLNVAFTSCGWIISLSTIFVGGSLAAGMTFGQAIL 68 + + ED+A+ +VP E+++S+ + G ++ ++ I+ GG L G+ IL Sbjct: 2 SDKTNQADVFEDHAVSAVPKEQQRSFTGMFMVYTGVLVCMAVIWTGGELGRGLALNNMIL 61 Query: 69 AAVIGMTILAVYGFFQGWMGAKYGVATAVLARQAFGRAGAGLFGILLS-LTMGIGWFGWQ 127 A + G I A+ G G +G +T V+ R +FGR G+ + G +S ++ GIGWF Q Sbjct: 62 AILGGSVITAIIGLLTGIIGGYTRTSTYVILRHSFGRYGSMVAGAAVSGISCGIGWFFIQ 121 Query: 128 VAFFGSTIEQMFPGQWFADP-----TFAIIWGGVLMIFTALIGYKGITYLSFVAVPLVIV 182 F +E + W + P + WG +LM TA+ G+KG+ LS+V VPL IV Sbjct: 122 AWLFSEVMETISLELWGSVPYLFQGHISAFWGAILMTLTAIFGFKGLALLSYVTVPLFIV 181 Query: 183 LSIWGIFVAADASGGLSNLFALQPTGASLGLFAGITLVVGNAALGAIVFPDVSRYGKTPL 242 + G A +GG S + P + + A IT +VG+ GA + PD++RY P Sbjct: 182 ILGGGSVAAITEAGGFSQIAQATP-AEPMTIGAAITAIVGSYIAGATISPDIARYAAKPT 240 Query: 243 AGGMGAAVGYFVGGIFSVVAGAAMTFAAQLPDLGATPNIPAVMAKLGMGFFAFLILVFAQ 302 GG +F +F + A L L ++ MA LG+G A LI++ Q Sbjct: 241 HGGW----AWFWQVLF--MQPLLFLAAGLLTLLTPQADVAEAMAHLGIGVGALLIVILGQ 294 Query: 303 WSNNDNNLYTGALGLRNVVKLPKFXXXXXXXXXXXXXXXTGY-----QDHFVPFLNFLGV 357 W+ NDNNLY GAL N +K+ K D F+ FLN LG Sbjct: 295 WTTNDNNLYNGALAFANTIKVKKAVITGILGFVGAILASLTALGVFGADPFMTFLNQLGR 354 Query: 358 YVPPIAGVMIADHWIVR---------RGTYQFGEGTKYSXXXXXXXXXXXXXXXXXXXXX 408 ++PPIAG++IAD +I++ YQFG GT+Y+ Sbjct: 355 FLPPIAGILIADFYILKPYLQGKKDPENRYQFGPGTEYACINWVGILSWIGAGYFSVYLP 414 Query: 409 WGISPINSTVLGLIIYPVLTALLRLLRIPYEWGQTTEDSTGY 450 GI +NS +L I + +LT + I Y G E+STG+ Sbjct: 415 -GIVALNSILLSFIAFIILTYTCEMFNIQYTSGSYVEESTGF 455 >Q9KBP3.1 Q9KBP3_BACHD Cytosine permease Length = 445 Score = 190 bits (482), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 134/379 (35%), Positives = 211/379 (55%), Gaps = 18/379 (4%) Query: 10 GRQGNIAALEDYAIQSVPAEKRKSWLNVAFTSCGWIISLSTIFVGGSLAAGMTFGQAILA 69 +Q ++D+ VP E RK W++++ G I+LS +GG+L G+T GQAILA Sbjct: 2 NKQSEARKIDDFERMPVPEEHRKGWIHLSLVWLGIAIALSATVLGGTLGGGLTLGQAILA 61 Query: 70 AVIGMTILAVYGFFQGWMGAKYGVATAVLARQAFGRAGAGLFGILLSLTMGIGWFGWQVA 129 ++G +L++ +GA+ G++T ++++ A G GA + ++++ + GWFG Q+ Sbjct: 62 TMLGALVLSIVSACCSVVGARTGLSTGLVSKFALGTYGAYVVSAVIAIAL-FGWFGVQLD 120 Query: 130 FFGSTIEQMFPGQWFAD--PTFAIIWGGVLMIFTALIGYKGITYLSFVAVP---LVIVLS 184 FG++ +Q+ + + +I GG+LM TA+IGYK I LS AVP L+++ S Sbjct: 121 LFGTSFQQVIMNVFGLNIPKILLVIIGGILMTSTAVIGYKAIEKLSKAAVPLMGLLLIAS 180 Query: 185 IWGIFVAADASGGLSNLFALQPTGASLGLFAGITLVVGNAALGAIVFPDVSRYGKTPLAG 244 +W + +S S++ TG L L I+LV+G+ A+GA++ PD++RY KTP Sbjct: 181 LWRVL----SSQQWSDVVNAPMTGDPLPLGIAISLVIGSFAVGAVIGPDIARYAKTPKDA 236 Query: 245 GMGAAVGYFVGGIFSVVAGAAMTFAAQLPDLGATPNIPAVMAKLGMGFFAFLILVFAQWS 304 +G+ G+ VG FS A + AA L +I A+M LG G FA LIL+ AQW+ Sbjct: 237 VIGSFSGFLVG--FS----AVLIVAAILAKATGEVDIVAIMLGLGWGTFAMLILILAQWT 290 Query: 305 NNDNNLYTGALGLRNVVK-LPKFXXXXXXXXXXXXXXXTGYQDHFVPFLNFLGVYVPPIA 363 NDNNLY+ ALG + K +PK G D+F+PFL FL +PPI Sbjct: 291 TNDNNLYSAALGFSVIFKSIPKAKLTIGAGIIGTLMAVFGIYDNFIPFLIFLSALIPPIG 350 Query: 364 GVMIADHWIVRRGTYQFGE 382 G+ +AD+ ++ +G Y F Sbjct: 351 GIYVADY-LLNKGKYSFAN 368 >C0BX33.1 C0BX33_9CLOT Putative uncharacterized protein Length = 445 Score = 181 bits (459), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 133/379 (35%), Positives = 198/379 (52%), Gaps = 28/379 (7%) Query: 19 EDYAIQSVPAEKRKSWLNVAFTSCGWIISLSTIFVGGSLAAGMTFGQAILAAVIGMTILA 78 +D+A + VP +K++ W + A G IS+S +GGSLA G+T ++I+A ++G ILA Sbjct: 18 DDFARKPVPEDKKRGWASSAVVYIGCAISISAFTLGGSLATGLTLNESIIAVLVGALILA 77 Query: 79 VYGFFQGWMGAKYGVATAVLARQAFGRAGAGLFGILLSLTMGIGWFGWQVAFFGSTIEQM 138 G++T+++ + FG GA L G+L + GWFG Q FG TI QM Sbjct: 78 AMACLCAEPAMYTGLSTSMIGKFTFGLRGAALVGLLYA-GCAWGWFGVQAGLFGDTIGQM 136 Query: 139 F---PGQWFADPTFA--IIWGGVLMIFTALIGYKGITYLSFVAVPLVIVLSIWGIFVAAD 193 F G + T I+ GG+LM +A+ G+K I LS+VA+P + +L + + + Sbjct: 137 FRLMTGSALSPFTVKVIIVIGGLLMTTSAIFGFKSIEKLSWVAIPAIAILMVSSLVMVLK 196 Query: 194 AS--GGLSNLFALQPTGASLGLFAGITLVVGNAALGAIVFPDVSRYGK----TPLAGGMG 247 L+ F P SLG GI+LV+G+ A GA+ PD+ RY K T A +G Sbjct: 197 GHTYADLNTSFITNPM--SLG--TGISLVIGSFAAGAVGSPDILRYAKNFKDTVKAMVIG 252 Query: 248 AAVGYFVGGIFSVVAGAAMTFAAQLPDLGATPNIPAVMAKLGMGFFAFLILVFAQWSNND 307 +GY GI V+A A A + D NI VM LG G A ++LV AQW++ND Sbjct: 253 LVIGY---GITIVIAAAC---AKAVGD----ANIVTVMIGLGWGVLAMVVLVLAQWTSND 302 Query: 308 NNLYTGALGLRNVV-KLPKFXXXXXXXXXXXXXXXTGYQDHFVPFLNFLGVYVPPIAGVM 366 NN+Y+G+LGL V K+PK+ G + +PF + LG++ PP+ G Sbjct: 303 NNVYSGSLGLSVVFEKMPKYKLAIFTGVSGTIFAVIGLSNMMIPFFSVLGIFTPPLGGCY 362 Query: 367 IADHWIVRRGTYQFGEGTK 385 +AD ++ R+ Y F K Sbjct: 363 LADFYLCRK-FYSFDRLNK 380 >B0K7W7.1 B0K7W7_THEP3 Permease for cytosine/purines, uracil, thiamine, allantoin Length = 420 Score = 181 bits (458), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 124/372 (33%), Positives = 196/372 (52%), Gaps = 17/372 (4%) Query: 18 LEDYAIQSVPAEKRKSWLNVAFTSCGWIISLSTIFVGGSLAAGMTFGQAILAAVIGMTIL 77 +E +A+ +P +++ W ++A G +I +S++ VGG++ GM +AI+A IG TI+ Sbjct: 1 MEQHALDPIPESEKRPWYSIALIWAGSMICVSSLMVGGAIVQGMPLLEAIIAGAIGYTII 60 Query: 78 AVYGFFQGWMGAKYGVATAVLARQAFGRAGAG-LFGILLSLTMGIGWFGWQVAFFG---S 133 VY FQG GA GV T V + AFGR GA + +L+++ IGWFG Q + G S Sbjct: 61 IVYMTFQGMQGADLGVPTVVTSSSAFGREGARFIISTILAISC-IGWFGVQASVCGAAFS 119 Query: 134 TIEQMFPGQWFADPTFAIIWGGVLMIFTALIGYKGITYLSFVAVPLVIVLSIWGIFVAAD 193 I M+ G + ++W G++M+ TA+ GYK + YL+++A+P ++ LSI G+ + Sbjct: 120 QIMNMWLGIYIPVWLSGLVW-GIIMLVTAVYGYKALEYLNYIAIPALVFLSIVGL-IQVL 177 Query: 194 ASGGLSNLFALQPTGASLGLFAGITLVVGNAALGAIVFPDVSRYGKTPLAGGMGAAVGYF 253 + G+ NL P +A I L VG A+G ++ D SRY K+ VG + Sbjct: 178 TTFGIENLINYVPKDRYSWSYA-IGLAVGGFAVGGVIAADFSRYAKSRKDALTSGLVGVW 236 Query: 254 VGGIFSVVAGAAMTFAAQLPDLGATPNIPAVMAKLGMGFFAFLILVFAQWSNNDNNLYTG 313 G+F +V GA +T A T +I +A+LG+GF +IL+ A W+ N N Y+G Sbjct: 237 PAGMFLLVVGALLTVVA------GTYDITIALAQLGLGFVGAVILILATWTTNTVNAYSG 290 Query: 314 ALGLRNVVKLP---KFXXXXXXXXXXXXXXXTGYQDHFVPFLNFLGVYVPPIAGVMIADH 370 L + ++ + + G ++F FL L +PPIAG MIAD+ Sbjct: 291 GLAIVSLFNMENKLRPLMTFIAGAIGTVLAIMGILNYFTSFLTVLTASIPPIAGSMIADY 350 Query: 371 WIVRRGTYQFGE 382 WI ++G E Sbjct: 351 WICKKGNRNMWE 362 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.142 0.448 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,547,106,823 Number of extensions: 104313744 Number of successful extensions: 489788 Number of sequences better than 1.0e-05: 594 Number of HSP's gapped: 488683 Number of HSP's successfully gapped: 594 Length of query: 450 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 311 Effective length of database: 1,751,175,118 Effective search space: 544615461698 Effective search space used: 544615461698 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)