BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0447.0004.p (501 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q65J89.1 Q65J89_BACLD Putative uncharacterized protein 698 0.0 A5A672.1 A5A672_BACLD Hydantoin utilization protein A 696 0.0 B0UAR1.1 B0UAR1_METS4 5-oxoprolinase (ATP-hydrolyzing) EC=3.5.2.9 434 e-119 B8IJK0.1 B8IJK0_METNO Hydantoinase/oxoprolinase EC=3.5.2.9 426 e-117 C4CLI9.1 C4CLI9_9CHLR N-methylhydantoinase A/acetone carboxylase... 422 e-116 >Q65J89.1 Q65J89_BACLD Putative uncharacterized protein Length = 701 Score = 698 bits (1801), Expect = 0.0, Method: Compositional matrix adjust. Identities = 328/457 (71%), Positives = 389/457 (85%) Query: 1 MTAENNSWMLGIDVGGTFTDLAALDERGNITATKTPSTPDPSDGVIQGIEKLSRQLNISM 60 M E N W+ GIDVGGTFTDL A+++ G + +TKTPSTPD SDGV+ GI+K++ + I + Sbjct: 1 MMNEKNQWVFGIDVGGTFTDLVAVNKDGKMVSTKTPSTPDQSDGVMNGIKKMAEIIGIDV 60 Query: 61 LDFLTRCSLLVHGTTVATNTILEYNGAKVGLITTEGFRDEIEFRRAYKESVFSPRLPAPY 120 D L L+VHGTTVATN +LEYNGAKVGL+TTEGFRDEIEFRRAYKESVFSPRL APY Sbjct: 61 KDLLANSPLVVHGTTVATNALLEYNGAKVGLLTTEGFRDEIEFRRAYKESVFSPRLEAPY 120 Query: 121 PIVPRRLRIGVPERVGAQGQTIIPLDEAAVREAIRFFKEEKVEAIAVSFLFSFLDPAHEQ 180 IVPRR R+G+PER+ G + LDE AVR A+R F +E VEA+AV FLFSF++P+HE Sbjct: 121 QIVPRRYRVGIPERLDHTGNVVTKLDEEAVRRAVRGFIQEGVEAVAVCFLFSFMNPSHEI 180 Query: 181 KVKQIAQEEAPDIFVTLSSEVLPQIREFERVSTTIVNAYTAPAMKRYMERLDRRLREQQF 240 +VK+I QEEAP +FV+LS EVLPQIREFERVSTTIVNA+T P+M+ Y+ LD R RE F Sbjct: 181 RVKEIIQEEAPGMFVSLSYEVLPQIREFERVSTTIVNAFTGPSMQSYLNHLDERFREDGF 240 Query: 241 KGELFVMQSSGGVQNIVQSGQLAASCLLSGPAGGVTAAAFIGERIGYKDLITVDMGGTSY 300 GELFVMQS+GGVQN++QSG+ AA LLSGPAGGVTAA+FIGE+ GY+++ITVDMGGTSY Sbjct: 241 TGELFVMQSNGGVQNVIQSGKFAAGGLLSGPAGGVTAASFIGEKSGYENVITVDMGGTSY 300 Query: 301 DVSVIEQLQPTLTTESWISRYRVALPMLDIHTVGAGGGSIAWIDNGGMLQVGPRSAGSTP 360 DVSVIE+L P +TTE+WISRYRVALPM+DIHT+G+GGGSIAWID+GG LQVGPRSAGS P Sbjct: 301 DVSVIEKLNPAITTENWISRYRVALPMMDIHTIGSGGGSIAWIDSGGALQVGPRSAGSNP 360 Query: 361 GPACYGRGGTEPTVTDANVVLGYINPDNFLGGEMKLDRTLSEEAIRRAVAEPLGVSVIEA 420 GPACYGRGG EPTVTD NV LGYINPDNFLGGEMKLDR+L+E+AIR +AEPLG+S +EA Sbjct: 361 GPACYGRGGEEPTVTDVNVFLGYINPDNFLGGEMKLDRSLAEKAIRTRIAEPLGISTVEA 420 Query: 421 ALAISEIVNNNMSNAIRFVTTQRGYDPRKFALLAAGG 457 ALAIS+IVN++MSNA+ FVTTQRG+DPR FAL+A GG Sbjct: 421 ALAISQIVNSDMSNAVHFVTTQRGHDPRNFALMAVGG 457 >A5A672.1 A5A672_BACLD Hydantoin utilization protein A Length = 700 Score = 696 bits (1797), Expect = 0.0, Method: Compositional matrix adjust. Identities = 327/454 (72%), Positives = 388/454 (85%) Query: 4 ENNSWMLGIDVGGTFTDLAALDERGNITATKTPSTPDPSDGVIQGIEKLSRQLNISMLDF 63 E N W+ GIDVGGTFTDL A+++ G + +TKTPSTPD SDGV+ GI+K++ + I + D Sbjct: 3 EKNQWVFGIDVGGTFTDLVAVNKDGKMVSTKTPSTPDQSDGVMNGIKKMAEIIGIDVKDL 62 Query: 64 LTRCSLLVHGTTVATNTILEYNGAKVGLITTEGFRDEIEFRRAYKESVFSPRLPAPYPIV 123 L L+VHGTTVATN +LEYNGAKVGL+TTEGFRDEIEFRRAYKESVFSPRL APY IV Sbjct: 63 LANSPLVVHGTTVATNALLEYNGAKVGLLTTEGFRDEIEFRRAYKESVFSPRLEAPYQIV 122 Query: 124 PRRLRIGVPERVGAQGQTIIPLDEAAVREAIRFFKEEKVEAIAVSFLFSFLDPAHEQKVK 183 PRR R+G+PER+ G + LDE AVR A+R F +E VEA+AV FLFSF++P+HE +VK Sbjct: 123 PRRYRVGIPERLDHTGNVVTKLDEEAVRRAVRGFIQEGVEAVAVCFLFSFMNPSHEIRVK 182 Query: 184 QIAQEEAPDIFVTLSSEVLPQIREFERVSTTIVNAYTAPAMKRYMERLDRRLREQQFKGE 243 +I QEEAP +FV+LS EVLPQIREFERVSTTIVNA+T P+M+ Y+ LD R RE F GE Sbjct: 183 EIIQEEAPGMFVSLSYEVLPQIREFERVSTTIVNAFTGPSMQSYLNHLDERFREDGFTGE 242 Query: 244 LFVMQSSGGVQNIVQSGQLAASCLLSGPAGGVTAAAFIGERIGYKDLITVDMGGTSYDVS 303 LFVMQS+GGVQN++QSG+ AA LLSGPAGGVTAA+FIGE+ GY+++ITVDMGGTSYDVS Sbjct: 243 LFVMQSNGGVQNVIQSGKFAAGGLLSGPAGGVTAASFIGEKSGYENVITVDMGGTSYDVS 302 Query: 304 VIEQLQPTLTTESWISRYRVALPMLDIHTVGAGGGSIAWIDNGGMLQVGPRSAGSTPGPA 363 VIE+L P +TTE+WISRYRVALPM+DIHT+G+GGGSIAWID+GG LQVGPRSAGS PGPA Sbjct: 303 VIEKLNPAITTENWISRYRVALPMMDIHTIGSGGGSIAWIDSGGALQVGPRSAGSNPGPA 362 Query: 364 CYGRGGTEPTVTDANVVLGYINPDNFLGGEMKLDRTLSEEAIRRAVAEPLGVSVIEAALA 423 CYGRGG EPTVTD NV LGYINPDNFLGGEMKLDR+L+E+AIR +AEPLG+S +EAALA Sbjct: 363 CYGRGGEEPTVTDVNVFLGYINPDNFLGGEMKLDRSLAEKAIRTRIAEPLGISTVEAALA 422 Query: 424 ISEIVNNNMSNAIRFVTTQRGYDPRKFALLAAGG 457 IS+IVN++MSNA+ FVTTQRG+DPR FAL+A GG Sbjct: 423 ISQIVNSDMSNAVHFVTTQRGHDPRNFALMAVGG 456 >B0UAR1.1 B0UAR1_METS4 5-oxoprolinase (ATP-hydrolyzing) EC=3.5.2.9 Length = 692 Score = 434 bits (1115), Expect = e-119, Method: Compositional matrix adjust. Identities = 227/451 (50%), Positives = 310/451 (68%), Gaps = 2/451 (0%) Query: 8 WMLGIDVGGTFTDLAALDERGNITATKTPSTP-DPSDGVIQGIEKLSRQLNISMLDFLTR 66 + +GIDVGGTFTDLA +D G +T KTPSTP D S GV+ G+ L+ +L + + L+ Sbjct: 11 YRIGIDVGGTFTDLACVDPSGALTFVKTPSTPEDQSVGVLTGLGDLAARLGLDLAGLLSA 70 Query: 67 CSLLVHGTTVATNTILEYNGAKVGLITTEGFRDEIEFRRAYKESVFSPRLPAPYPIVPRR 126 +VHGTTVATN +LE GA+VGL+TT G RD +E R K + + +P P P+VPR Sbjct: 71 TDRIVHGTTVATNALLERKGARVGLLTTRGHRDVLEMREGLKPNRYDLLMPPPEPLVPRH 130 Query: 127 LRIGVPERVGAQGQTIIPLDEAAVREAIRFFKEEKVEAIAVSFLFSFLDPAHEQKVKQIA 186 LR GVPER+G G + PLDEAA+ E I + VEA+AV++L S+ +PAHE++ + Sbjct: 131 LRRGVPERIGHDGTVVTPLDEAALDEEIARLRSAGVEAVAVAYLHSYRNPAHERRTAERL 190 Query: 187 QEEAPDIFVTLSSEVLPQIREFERVSTTIVNAYTAPAMKRYMERLDRRLREQQFKGELFV 246 P+ +VTLSSEVLPQI+E+ERVSTT VNAY PA++RY+ L+RRL E +F+G LF+ Sbjct: 191 AALMPEAYVTLSSEVLPQIKEYERVSTTAVNAYVGPAVRRYLGSLERRLAEVRFEGGLFI 250 Query: 247 MQSSGGVQNIVQSGQLAASCLLSGPAGGVTAAAFIGERIGYKDLITVDMGGTSYDVSVIE 306 + S GGV + ++ +LAA+ +LSGPAGGV + + G +LI DMGGTS D+S++ Sbjct: 251 ILSQGGVVPLGEAARLAAATVLSGPAGGVAGSRHVAALTGACNLIPFDMGGTSTDISLVA 310 Query: 307 QLQPTLTTESWISRYRVALPMLDIHTVGAGGGSIAWIDNGGMLQVGPRSAGSTPGPACYG 366 L+ + ++ R+AL LDI ++GAGGGSIA +D GG+LQVGP SAG+ PGP CY Sbjct: 311 DGAIALSADGGLAGERIALRSLDIISIGAGGGSIAHVDGGGILQVGPESAGAKPGPVCYS 370 Query: 367 RGGTEPTVTDANVVLGYINPDNFLGGEMKLDRTLSEEAIRRAVAEPLGVSVIEAALAISE 426 +GGTEPTVTDAN++LGY++P++FLGG +LD + A+ R A LG S EAA I Sbjct: 371 QGGTEPTVTDANLLLGYLDPEHFLGGRRRLDAAGAAAAMDRLAAS-LGASRDEAAAGIYR 429 Query: 427 IVNNNMSNAIRFVTTQRGYDPRKFALLAAGG 457 +VN M++ IR +T +RG DPR FA+L+ GG Sbjct: 430 LVNLKMADGIRLMTLRRGVDPRGFAVLSFGG 460 >B8IJK0.1 B8IJK0_METNO Hydantoinase/oxoprolinase EC=3.5.2.9 Length = 686 Score = 426 bits (1095), Expect = e-117, Method: Compositional matrix adjust. Identities = 225/454 (49%), Positives = 309/454 (68%), Gaps = 2/454 (0%) Query: 5 NNSWMLGIDVGGTFTDLAALDERGNITATKTPSTP-DPSDGVIQGIEKLSRQLNISMLDF 63 + ++ +GIDVGGTFTDLA +D+ G +T KTPSTP D S GV+ G+ L+ +L + + Sbjct: 2 SKTYRIGIDVGGTFTDLACVDDAGTLTFVKTPSTPEDQSVGVLTGLGDLADRLGVDLAGL 61 Query: 64 LTRCSLLVHGTTVATNTILEYNGAKVGLITTEGFRDEIEFRRAYKESVFSPRLPAPYPIV 123 L+ +VHGTTVATN +LE GAKVGL+TT G RD +E R K ++ +P P P+V Sbjct: 62 LSATDRIVHGTTVATNALLERKGAKVGLLTTRGHRDVLEMREGLKPDRYNLLMPPPEPLV 121 Query: 124 PRRLRIGVPERVGAQGQTIIPLDEAAVREAIRFFKEEKVEAIAVSFLFSFLDPAHEQKVK 183 PR+LR GV ERV G + LDEA + I + VEA+AV++L S+ +PAHE++ Sbjct: 122 PRQLRRGVTERVAHDGTVLAALDEAELDAEIARLRSAGVEAVAVAYLHSYRNPAHERRTA 181 Query: 184 QIAQEEAPDIFVTLSSEVLPQIREFERVSTTIVNAYTAPAMKRYMERLDRRLREQQFKGE 243 + PD +VTLSSEVLPQI+EFERVSTT+VNAY PA++RY+ L+RRL E +F+G Sbjct: 182 ERLAALMPDAYVTLSSEVLPQIKEFERVSTTVVNAYVGPAVRRYLASLERRLAEIRFEGA 241 Query: 244 LFVMQSSGGVQNIVQSGQLAASCLLSGPAGGVTAAAFIGERIGYKDLITVDMGGTSYDVS 303 LF++ S GGV + ++ +LAA+ +LSGPAGGV + ++ G ++LI DMGGTS D+S Sbjct: 242 LFIILSQGGVVPLREAARLAAATVLSGPAGGVAGSRYVAALTGARNLIPFDMGGTSTDIS 301 Query: 304 VIEQLQPTLTTESWISRYRVALPMLDIHTVGAGGGSIAWIDNGGMLQVGPRSAGSTPGPA 363 ++ L+ ++ R+AL LDI ++GAGGGSIA +D G+LQVGP SAGS PGP Sbjct: 302 LVADGSIALSAAGGLAGERIALRSLDIISIGAGGGSIARVDASGILQVGPDSAGSKPGPV 361 Query: 364 CYGRGGTEPTVTDANVVLGYINPDNFLGGEMKLDRTLSEEAIRRAVAEPLGVSVIEAALA 423 CYG+GGTEPTVTDAN++LGY++ D+FLGG +LD + + R A LG + EAA Sbjct: 362 CYGQGGTEPTVTDANLLLGYLDADHFLGGRRRLDEAGAIAVMDRLAAR-LGTTRDEAAAG 420 Query: 424 ISEIVNNNMSNAIRFVTTQRGYDPRKFALLAAGG 457 I +VN M++ IR +T +RG DPR FA+L+ GG Sbjct: 421 IYRLVNLKMADGIRLMTLRRGVDPRGFAILSFGG 454 >C4CLI9.1 C4CLI9_9CHLR N-methylhydantoinase A/acetone carboxylase, beta subunit Length = 686 Score = 422 bits (1084), Expect = e-116, Method: Compositional matrix adjust. Identities = 231/452 (51%), Positives = 294/452 (65%), Gaps = 9/452 (1%) Query: 10 LGIDVGGTFTDLAALDE-RGNITATKTPSTP-DPSDGVIQGIEKLSRQLNISMLDFLTRC 67 +GID GGTFTD+ A E G I A K PSTP +P++ I G+ +L S D Sbjct: 5 IGIDTGGTFTDVIAFREADGRIVALKVPSTPQNPAEAFIAGVRAALEELGASPGD----V 60 Query: 68 SLLVHGTTVATNTILEYNGAKVGLITTEGFRDEIEFRRAYK--ESVFSPRLPAPYPIVPR 125 + L HGTTVATN +LE GA+ GLITTEGFRD E RR + E +++ P P+VPR Sbjct: 61 AFLAHGTTVATNALLEGKGARTGLITTEGFRDVYEIRRLNRSFEDLYNIFWTKPTPLVPR 120 Query: 126 RLRIGVPERVGAQGQTIIPLDEAAVREAIRFFKEEKVEAIAVSFLFSFLDPAHEQKVKQI 185 LR V ERV G ++ LD VR+A EE VE++AV FL S+L+P HE++ I Sbjct: 121 YLRKEVRERVNVDGVAVVSLDADGVRDAAAALGEEGVESVAVCFLHSYLNPEHERQAAAI 180 Query: 186 AQEEAPDIFVTLSSEVLPQIREFERVSTTIVNAYTAPAMKRYMERLDRRLREQQFKGELF 245 +E P + ++LS EV P+ RE+ER STTIVNA+ AP ++RY+ L+RRL E L Sbjct: 181 IREVLPGVSISLSHEVNPEYREYERTSTTIVNAFVAPIIERYLTDLERRLAETGVSAALH 240 Query: 246 VMQSSGGVQNIVQSGQLAASCLLSGPAGGVTAAAFIGERIGYKDLITVDMGGTSYDVSVI 305 VM S G Q + + +LSGPA GV A +G IG DLIT+DMGGTS DV+++ Sbjct: 241 VMHSGGAAQTGRAARSRPITTILSGPAAGVIGAEALGREIGVSDLITLDMGGTSTDVALL 300 Query: 306 EQLQPTLTTESWISRYRVALPMLDIHTVGAGGGSIAWIDNGGMLQVGPRSAGSTPGPACY 365 Q +PTL +ES ++ + MLDI T+GAGGGSIAW D GG L+VGP+SAG+ PGPACY Sbjct: 301 RQGKPTLASESELAGEVIRAAMLDITTIGAGGGSIAWADEGGRLKVGPQSAGADPGPACY 360 Query: 366 GRGGTEPTVTDANVVLGYINPDNFLGGEMKLDRTLSEEAIRRAVAEPLGVSVIEAALAIS 425 GRGGT+PTVTDAN+VLGY+ D FLGG M+LDR +E A+ R AE LG+S +E A I Sbjct: 361 GRGGTQPTVTDANLVLGYLGTDAFLGGRMRLDRAAAEAAVDRLAAE-LGLSRLETAHGIF 419 Query: 426 EIVNNNMSNAIRFVTTQRGYDPRKFALLAAGG 457 I N NM AIR V+ +RG DPR+FALLA GG Sbjct: 420 RIANANMLRAIRLVSVERGLDPRRFALLAFGG 451 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,161,552,817 Number of extensions: 139537345 Number of successful extensions: 394894 Number of sequences better than 1.0e-05: 977 Number of HSP's gapped: 389915 Number of HSP's successfully gapped: 995 Length of query: 501 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 361 Effective length of database: 1,741,754,103 Effective search space: 628773231183 Effective search space used: 628773231183 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)