BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0444.0031.p (389 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q97MJ4.1 Q97MJ4_CLOAB Hydrolase of the alpha/beta superfamily 454 e-126 Q24TL1.1 Q24TL1_DESHY Putative uncharacterized protein 238 5e-61 B8FHP4.1 B8FHP4_DESAA Putative uncharacterized protein 232 5e-59 Q5EVE7.1 Q5EVE7_DESHY Putative hydrolase 201 1e-49 B8FG48.1 B8FG48_DESAA Alpha/beta hydrolase fold protein 197 2e-48 >Q97MJ4.1 Q97MJ4_CLOAB Hydrolase of the alpha/beta superfamily Length = 388 Score = 454 bits (1167), Expect = e-126, Method: Compositional matrix adjust. Identities = 216/378 (57%), Positives = 281/378 (74%), Gaps = 5/378 (1%) Query: 11 FPVGFYEELHPDFSINFQMNRFYSWTNDLEMLEEMRAAAPGIHNYAELIEICLKLGQQAV 70 FPVGFY+++HPDFS+NFQMNR Y+ TND ML+EMR +P IHNY E IE L+L +A+ Sbjct: 11 FPVGFYDDIHPDFSVNFQMNRCYTMTNDDVMLKEMREISPSIHNYEEFIEKFLELYNKAL 70 Query: 71 AADKKLKAANYLRGAEFYMPEGHPLKHTSRQQFIVLTRAHYQVEDNQHFDIPYGKGFLSA 130 KL+AA YLR AEFYM HP K R+QFI L + ++ + D Q + +PY GFL A Sbjct: 71 NEGHKLRAAYYLRTAEFYMTADHPQKQNYRKQFISLMQEYFNISDEQPYKVPYENGFLPA 130 Query: 131 YRFSPEVPAKGSIVLFGGFDSYIEEIFLMALVFKDAGYDVVCFDGPGQGAALEDWQLPMT 190 YRF+ E P KG+IV+FGGFDSY+EE+F MAL K+AGYDVV FDGPGQG+ALED+++PMT Sbjct: 131 YRFTSEAP-KGTIVMFGGFDSYVEEMFKMALFLKEAGYDVVFFDGPGQGSALEDYKIPMT 189 Query: 191 HEWEKPVATVLDFFNLQEVTLIXXXXXXXXXXXXXXYETNRVKRVVADDICTDFYKVLLR 250 HEWEKPV ++LD+F L +VTL+ +E RV RV+ADDI ++FY+V+L Sbjct: 190 HEWEKPVKSILDYFELDDVTLLGISLGGYLTVRAAAFE-KRVHRVIADDILSNFYEVVL- 247 Query: 251 QTGP--LEDSISTLMAKENEAEINALFSRLMKENLMLEWGITQGMHVTGSQTPYGFLKQS 308 T P L++ +++LM+ NE E+N LF LMK++LM+EW I QGMH+TGS+TPY FL + Sbjct: 248 NTLPSQLKEKVNSLMSDGNEQEVNILFKDLMKKSLMVEWAINQGMHITGSKTPYEFLNKI 307 Query: 309 MLYTTAGISPLLSQDVLLLAAQEDHYIPLVQFGEQTGSLTSVRSLTTRLFTRKEQAQNHC 368 +LY T+ IS L QDVLL+AAQEDHYIPL QF +Q+ SL +VRSLTTR+FTRKE AQNHC Sbjct: 308 LLYNTSKISEKLEQDVLLMAAQEDHYIPLNQFFDQSKSLINVRSLTTRMFTRKETAQNHC 367 Query: 369 HVGNIGLSIHVMLHWLQQ 386 GN+GL+ V+ +W++Q Sbjct: 368 QSGNLGLAFEVIANWIEQ 385 >Q24TL1.1 Q24TL1_DESHY Putative uncharacterized protein Length = 388 Score = 238 bits (608), Expect = 5e-61, Method: Compositional matrix adjust. Identities = 141/390 (36%), Positives = 216/390 (55%), Gaps = 17/390 (4%) Query: 8 TADFPVGFYEELHPDFSINFQMNRFYSWTNDLEMLEEMRAAAPGIHNYAELIEICLKLGQ 67 T +F G Y LH D + N+Q+NR + L+E+R+ I + IE L L Sbjct: 3 TLEFRTGTYP-LHHDPNFNYQLNRAVMLSGG--ALDEIRSVTNQIKDIPTWIETLLSLAD 59 Query: 68 QAVAADKKLKAANYLRGAEFYMPEGHPLKHTSRQQFIVLTRAHYQVEDN-------QHFD 120 +A A + A Y+R AEF+M E + K T +++ L Y+V + D Sbjct: 60 KAKAEKRTPNALAYIRAAEFFMNESNTAKETLYREYKAL---FYEVHGGFIASQGIKRID 116 Query: 121 IPYGKGFLSAYRFSPEVPAKGSIVLFGGFDSYIEEIFLMALVF-KDAGYDVVCFDGPGQG 179 I Y KG L + +PE + G++V+ GGFDSY+EE FL AL++ K+ G+ V F+GPGQG Sbjct: 117 IGYEKGSLPVFYAAPETGSLGTLVIHGGFDSYMEE-FLPALLYCKELGFAVYLFEGPGQG 175 Query: 180 AALEDWQLPMTHEWEKPVATVLDFFNLQEVTLIXXXXXXXXXXXXXXYETNRVKRVVADD 239 + ++P T +W KPV VL++F L++VTLI +E R+KRVVA Sbjct: 176 ECIHKQKIPFTLDWHKPVGAVLNYFGLEDVTLIGISLGSLLALRAAAWE-KRIKRVVAWS 234 Query: 240 ICTDFYKVLLR-QTGPLEDSISTLMAKENEAEINALFSRLMKENLMLEWGITQGMHVTGS 298 I +FY VLL + L + +L+ + +N+L +M++ WGI QG +V G Sbjct: 235 IMENFYDVLLSSRPKALRVVLDSLIKAHAKGAVNSLAKTMMEKEPYAAWGIEQGKYVFGQ 294 Query: 299 QTPYGFLKQSMLYTTAGISPLLSQDVLLLAAQEDHYIPLVQFGEQTGSLTSVRSLTTRLF 358 + Y FL ++ Y +S ++QDVLLL A EDH+IPL + + LT V+SLT RLF Sbjct: 295 PSAYDFLAEAKKYNLCSLSGQITQDVLLLGAAEDHFIPLSMYKNEIDCLTKVKSLTFRLF 354 Query: 359 TRKEQAQNHCHVGNIGLSIHVMLHWLQQWE 388 T++E A++HC++GN+ L+++ + W+ E Sbjct: 355 TKEESAESHCNIGNMPLALNTICSWITAME 384 >B8FHP4.1 B8FHP4_DESAA Putative uncharacterized protein Length = 389 Score = 232 bits (591), Expect = 5e-59, Method: Compositional matrix adjust. Identities = 146/384 (38%), Positives = 206/384 (53%), Gaps = 15/384 (3%) Query: 11 FPVGFYEELHPDFSINFQMNRFYSWTNDLEMLEEMRAAAPGIHNYAELIEICLKLGQQAV 70 F VG Y+ HP+ +INFQ+NR S +E++RA + I N + L L + A Sbjct: 5 FRVGTYD-FHPESNINFQLNRQISLGGG--RMEDIRAVSSKIKNLDDWKREFLGLARLAE 61 Query: 71 AADKKLKAANYLRGAEFYMPEGHPLKHTSRQQFIVLTRAHYQVEDNQHFDI------PYG 124 ++ AA+Y RGAEF+M G P K + Q L + V+D+ PY Sbjct: 62 QEERWENAASYYRGAEFFMTPGDPDKEWAYDQAAALFQK--IVKDDLESGALKVEAAPYE 119 Query: 125 KGFLSAYRF-SPE-VPAKGSIVLFGGFDSYIEEIFLMALVFKDAGYDVVCFDGPGQGAAL 182 L +R +PE V KG+IVL GG+DS EE++ A + GYDV F+GPGQG AL Sbjct: 120 TSRLPVFRLPAPEGVEFKGTIVLHGGYDSSKEELYPSADFVRKQGYDVYLFEGPGQGEAL 179 Query: 183 EDWQLPMTHEWEKPVATVLDFFNLQEVTLIXXXXXXXXXXXXXXYETNRVKRVVADDICT 242 L MT+EWEKPV VLD + L+ V L+ +E R+ RVVA + Sbjct: 180 VKNGLYMTYEWEKPVGAVLDHYGLEGVALVGASLGGYLAPRAAAFEP-RINRVVAWGVMY 238 Query: 243 DFYKVLLRQTG-PLEDSISTLMAKENEAEINALFSRLMKENLMLEWGITQGMHVTGSQTP 301 DF++VL G +E + +L+A + +N + + M+ + +WGI GM V G TP Sbjct: 239 DFFEVLTSVRGKAMEIGMKSLIALRAKKALNRMAALRMERDPFAKWGINHGMFVMGKPTP 298 Query: 302 YGFLKQSMLYTTAGISPLLSQDVLLLAAQEDHYIPLVQFGEQTGSLTSVRSLTTRLFTRK 361 Y FL+ + ++ G+S + QD LLLA DH+IPL + Q LT+VRS T R+FT Sbjct: 299 YDFLQYARNFSMKGVSGKVRQDFLLLAGTRDHFIPLKHYHRQARELTNVRSFTGRIFTEA 358 Query: 362 EQAQNHCHVGNIGLSIHVMLHWLQ 385 EQA+NHC GNI L +V+ W+ Sbjct: 359 EQAENHCQCGNIPLVFNVISEWMN 382 >Q5EVE7.1 Q5EVE7_DESHY Putative hydrolase Length = 308 Score = 201 bits (510), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 106/274 (38%), Positives = 163/274 (59%), Gaps = 4/274 (1%) Query: 117 QHFDIPYGKGFLSAYRFSPEVPAKGSIVLFGGFDSYIEEIFLMALVF-KDAGYDVVCFDG 175 + DI Y KG LS + +PE + G++V+ GGFDSY+EE FL AL++ K+ G+ V F+G Sbjct: 33 KRIDIGYEKGSLSVFYAAPETGSLGTLVIHGGFDSYMEE-FLPALLYCKELGFAVYLFEG 91 Query: 176 PGQGAALEDWQLPMTHEWEKPVATVLDFFNLQEVTLIXXXXXXXXXXXXXXYETNRVKRV 235 PGQG + ++P T +W KPV VL++F L++V LI +E R+KRV Sbjct: 92 PGQGECIHKQKIPFTLDWHKPVGAVLNYFGLEDVPLIGISLGSLLALRAAAWE-KRIKRV 150 Query: 236 VADDICTDFYKVLLR-QTGPLEDSISTLMAKENEAEINALFSRLMKENLMLEWGITQGMH 294 VA I +FY VLL + L + +L+ + +N+L +M++ WGI QG + Sbjct: 151 VAWSIMENFYDVLLSSRPKALRVVLDSLIKAHAKGAVNSLAKXMMEKEPYAAWGIEQGKY 210 Query: 295 VTGSQTPYGFLKQSMLYTTAGISPLLSQDVLLLAAQEDHYIPLVQFGEQTGSLTSVRSLT 354 V G + Y FL ++ Y +S ++QDVLLL A DH+IPL + + LT V+SLT Sbjct: 211 VFGQPSAYDFLAEAKKYNLCSLSGQITQDVLLLGAAXDHFIPLSMYKNEIDCLTKVKSLT 270 Query: 355 TRLFTRKEQAQNHCHVGNIGLSIHVMLHWLQQWE 388 RLFT++E A++HC++GN+ L+++ + W+ E Sbjct: 271 FRLFTKEESAESHCNIGNMPLALNTICSWITAME 304 >B8FG48.1 B8FG48_DESAA Alpha/beta hydrolase fold protein Length = 378 Score = 197 bits (501), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 128/383 (33%), Positives = 196/383 (51%), Gaps = 20/383 (5%) Query: 11 FPVGFYEELHPDFSINFQMNRFYSWTNDLEMLEEMRAAAPGIHNYAELIEICLKLGQQAV 70 FPVG Y +LH +FQ+NR++SW L++M+AAA I + + L A+ Sbjct: 6 FPVG-YHDLHKVKIFDFQLNRWHSWG--YTSLQDMKAAAAKIRKLDDWKDALLARMNIAL 62 Query: 71 AADKKLKAANYLRGAEFYMPEGHPLKHTSRQQFI-VLTRAHYQVEDNQHFDIPYGKGFLS 129 + L A R A+F++ P K FI + ++ E + IPY + Sbjct: 63 EQGRLLNAVFNCRAADFFVHPSDPDKKRIYDMFIDMFYNDLFKHESLERVWIPYEGAAIP 122 Query: 130 AYRFSPEVPA-----KGSIVLFGGFDSYIEEIFLMALVFKDAGYDVVCFDGPGQGAALED 184 A ++PA KG +V+ GGFDSY+EE+ A+ F + GYDV+ F+GPGQGAAL++ Sbjct: 123 AV----QIPASGDNSKGPMVIHGGFDSYMEELLSCAVYFSNRGYDVILFEGPGQGAALKE 178 Query: 185 WQLPMTHEWEKPVATVLDFFNLQEVTLIXXXXXXXXXXXXXXYETNRVKRVVADDICTDF 244 L + + WEKPV VLD F L+E L+ +E R+K+V+A I D+ Sbjct: 179 KGLYLDYRWEKPVKAVLDHFGLEECALLGFSMGGWFCFRAAAFEP-RIKQVIASSIAYDY 237 Query: 245 YKVLLRQTGPLEDSISTLMAKENEAEINALFSRLMKENLMLEWGITQGMHVTGSQTPYGF 304 ++ L+ + M E+++ + MK +WG+ M++TG + Sbjct: 238 MQI---PPKALQALANWFMKHPRLTEVSSRWK--MKFMPQEKWGVENFMYITGKNSIVDL 292 Query: 305 LKQSMLYTTAGI-SPLLSQDVLLLAAQEDHYIPLVQFGEQTGSLTSVRSLTTRLFTRKEQ 363 + + S L+ QDVL+L EDH+IPL Q +L + RS+ R+FTRKE Sbjct: 293 FHEMQKLNADNLKSELVKQDVLILTGDEDHFIPLKMHHMQVAALKNARSVEGRIFTRKEH 352 Query: 364 AQNHCHVGNIGLSIHVMLHWLQQ 386 AQNHC +GN GL + V+ WL++ Sbjct: 353 AQNHCMIGNTGLMLAVIKAWLEK 375 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.136 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,274,977,327 Number of extensions: 90702793 Number of successful extensions: 174209 Number of sequences better than 1.0e-05: 56 Number of HSP's gapped: 174126 Number of HSP's successfully gapped: 56 Length of query: 389 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 251 Effective length of database: 1,760,596,133 Effective search space: 441909629383 Effective search space used: 441909629383 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)