BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0444.0030.p (188 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C2W9M6.1 C2W9M6_BACCE Transcriptional regulator, TetR 147 6e-34 C2QZ56.1 C2QZ56_BACCE Transcriptional regulator, TetR 124 3e-27 Q81L20.1 Q81L20_BACAN Transcriptional regulator, TetR family 123 9e-27 Q6HCW0.1 Q6HCW0_BACHK Transcriptional regulator, TetR family 123 9e-27 Q633M6.1 Q633M6_BACCZ Transcriptional regulator, TetR family 123 9e-27 >C2W9M6.1 C2W9M6_BACCE Transcriptional regulator, TetR Length = 204 Score = 147 bits (370), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 73/190 (38%), Positives = 106/190 (55%), Gaps = 6/190 (3%) Query: 1 VKTKKLIRDALVSLIAEKGFDSVTVNDIAGRAQINRSTFYLHYTDKYELLEKITAEITGK 60 ++TKK I DA + LIAEKG+D++T+ DIA A INR+TFY HY DK +LL K++ + Sbjct: 10 IRTKKFINDAFIKLIAEKGYDAITIQDIADEALINRATFYSHYRDKQDLLAKLSEGALEE 69 Query: 61 LMALIIPAAHVQGGLLGVGDLTRDLEKILAAIAEDALFYQIMLSGRGVHSFRFDLGNLLK 120 L + ++ AHVQG + + L L+ I + + A FY+ M G+H F + + Sbjct: 70 LTSKMVLYAHVQGHQIDLSKLEEVLQSIFECMVKHADFYKTMFGPHGIHDFNLQMQQTIM 129 Query: 121 QKLDEGFREQPL------ISKDLFVELLSSLYLGAIAWWLDNGMKYSPAFMAGQLVQLMT 174 L+ + E + I KD+ + +S +G WWL N MKYSP + A QLV+LMT Sbjct: 130 DNLERNYIELKIGEHDSDIPKDMVLRFFTSCVVGMAIWWLQNDMKYSPKYTAQQLVKLMT 189 Query: 175 MGPVKVSGLV 184 GP G Sbjct: 190 KGPFGAVGFT 199 >C2QZ56.1 C2QZ56_BACCE Transcriptional regulator, TetR Length = 205 Score = 124 bits (312), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 67/189 (35%), Positives = 108/189 (57%), Gaps = 7/189 (3%) Query: 2 KTKKLIRDALVSLIAEKGFDSVTVNDIAGRAQINRSTFYLHYTDKYELLEKITAEITGKL 61 +T++LI+DA V+L+ EKGF++VTV IA RA +NR+TFY HY DKY+LL+K E+ KL Sbjct: 13 RTRQLIQDAFVALVGEKGFENVTVQHIAERAPVNRATFYSHYHDKYDLLDKSIEEMLEKL 72 Query: 62 MALIIPAAHVQGGL-LGVGDLTRDLEKILAAIAEDALFYQIMLSGRGVHSFRFDLGNLLK 120 +I P + L + + IAE+A FY +ML + V ++ + + ++ Sbjct: 73 TEVIKPKNRNKEDFQLAFDSPHPNFLALFEHIAENANFYNVMLGDKAVGNYTYKMMKAIQ 132 Query: 121 QKL------DEGFREQPLISKDLFVELLSSLYLGAIAWWLDNGMKYSPAFMAGQLVQLMT 174 L + E+ ++ +D+ + ++ +LG I WL GM Y+P FMA QL +L+ Sbjct: 133 THLTLSLSISQPNDEKLMVPRDILISYVTGAHLGMIMSWLKRGMIYTPHFMAMQLTRLII 192 Query: 175 MGPVKVSGL 183 +G +GL Sbjct: 193 LGAHTAAGL 201 >Q81L20.1 Q81L20_BACAN Transcriptional regulator, TetR family Length = 205 Score = 123 bits (308), Expect = 9e-27, Method: Compositional matrix adjust. Identities = 66/189 (34%), Positives = 107/189 (56%), Gaps = 7/189 (3%) Query: 2 KTKKLIRDALVSLIAEKGFDSVTVNDIAGRAQINRSTFYLHYTDKYELLEKITAEITGKL 61 +T++LI+DA V+L+ EKGF++VTV IA RA +NR+TFY HY DKY+LL+K E+ KL Sbjct: 13 RTRQLIQDAFVALVGEKGFENVTVQHIAERAPVNRATFYSHYHDKYDLLDKSIEEMLEKL 72 Query: 62 MALIIPAAHVQGGL-LGVGDLTRDLEKILAAIAEDALFYQIMLSGRGVHSFRFDLGNLLK 120 +I P + L + + IAE+A FY +ML + ++ + + ++ Sbjct: 73 TEVIKPKNRNKEDFQLAFDSPHPNFLALFEHIAENANFYNVMLGDKAAGNYTYKMMKAIQ 132 Query: 121 QKL------DEGFREQPLISKDLFVELLSSLYLGAIAWWLDNGMKYSPAFMAGQLVQLMT 174 L + E+ ++ +D+ + ++ +LG I WL GM Y+P FMA QL +L+ Sbjct: 133 THLTLSLSISQPNDEKLMVPRDILISYVTGAHLGMIMSWLKRGMIYTPHFMAMQLTRLII 192 Query: 175 MGPVKVSGL 183 +G +GL Sbjct: 193 LGAHTAAGL 201 >Q6HCW0.1 Q6HCW0_BACHK Transcriptional regulator, TetR family Length = 205 Score = 123 bits (308), Expect = 9e-27, Method: Compositional matrix adjust. Identities = 66/189 (34%), Positives = 107/189 (56%), Gaps = 7/189 (3%) Query: 2 KTKKLIRDALVSLIAEKGFDSVTVNDIAGRAQINRSTFYLHYTDKYELLEKITAEITGKL 61 +T++LI+DA V+L+ EKGF++VTV IA RA +NR+TFY HY DKY+LL+K E+ KL Sbjct: 13 RTRQLIQDAFVALVGEKGFENVTVQHIAERAPVNRATFYSHYHDKYDLLDKSIEEMLEKL 72 Query: 62 MALIIPAAHVQGGL-LGVGDLTRDLEKILAAIAEDALFYQIMLSGRGVHSFRFDLGNLLK 120 +I P + L + + IAE+A FY +ML + ++ + + ++ Sbjct: 73 TEVIKPKNRNKEDFQLAFDSPHPNFLALFEHIAENANFYNVMLGDKAAGNYTYKMMKAIQ 132 Query: 121 QKL------DEGFREQPLISKDLFVELLSSLYLGAIAWWLDNGMKYSPAFMAGQLVQLMT 174 L + E+ ++ +D+ + ++ +LG I WL GM Y+P FMA QL +L+ Sbjct: 133 THLTLSLSISQPNDEKLMVPRDILISYVTGAHLGMIMSWLKRGMIYTPHFMAMQLTRLII 192 Query: 175 MGPVKVSGL 183 +G +GL Sbjct: 193 LGAHTAAGL 201 >Q633M6.1 Q633M6_BACCZ Transcriptional regulator, TetR family Length = 205 Score = 123 bits (308), Expect = 9e-27, Method: Compositional matrix adjust. Identities = 66/189 (34%), Positives = 107/189 (56%), Gaps = 7/189 (3%) Query: 2 KTKKLIRDALVSLIAEKGFDSVTVNDIAGRAQINRSTFYLHYTDKYELLEKITAEITGKL 61 +T++LI+DA V+L+ EKGF++VTV IA RA +NR+TFY HY DKY+LL+K E+ KL Sbjct: 13 RTRQLIQDAFVALVGEKGFENVTVQHIAERAPVNRATFYSHYHDKYDLLDKSIEEMLEKL 72 Query: 62 MALIIPAAHVQGGL-LGVGDLTRDLEKILAAIAEDALFYQIMLSGRGVHSFRFDLGNLLK 120 +I P + L + + IAE+A FY +ML + ++ + + ++ Sbjct: 73 TEVIKPKNRNKEDFQLAFDSPHPNFLALFEHIAENANFYNVMLGDKAAGNYTYKMMKAIQ 132 Query: 121 QKL------DEGFREQPLISKDLFVELLSSLYLGAIAWWLDNGMKYSPAFMAGQLVQLMT 174 L + E+ ++ +D+ + ++ +LG I WL GM Y+P FMA QL +L+ Sbjct: 133 THLTLSLSISQPNDEKLMVPRDILISYVTGAHLGMIMSWLKRGMIYTPHFMAMQLTRLII 192 Query: 175 MGPVKVSGL 183 +G +GL Sbjct: 193 LGAHTAAGL 201 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.140 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,050,811,119 Number of extensions: 42363104 Number of successful extensions: 122911 Number of sequences better than 1.0e-05: 715 Number of HSP's gapped: 122653 Number of HSP's successfully gapped: 716 Length of query: 188 Length of database: 3,060,696,203 Length adjustment: 128 Effective length of query: 60 Effective length of database: 1,854,806,283 Effective search space: 111288376980 Effective search space used: 111288376980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)