BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0444.0018.p (147 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DMK2.1 B1DMK2_9BACL Glyoxalase/bleomycin resistance protein/di... 180 3e-44 B1HNS4.1 B1HNS4_LYSSC Protein phnB 178 1e-43 A3IEW3.1 A3IEW3_9BACI Uncharacterized conserved protein, phnB fa... 177 3e-43 Q97D00.1 Q97D00_CLOAB Uncharacterized conserved protein, phnB fa... 167 2e-40 C4VD15.1 C4VD15_ENTFA Protein PhnB 144 3e-33 >B1DMK2.1 B1DMK2_9BACL Glyoxalase/bleomycin resistance protein/dioxygenase Length = 140 Score = 180 bits (457), Expect = 3e-44, Method: Compositional matrix adjust. Identities = 80/139 (57%), Positives = 105/139 (75%) Query: 4 VAINVYFNFNGNAREAVEFYAEVFGTEAPRIMTFGEAPPDPSHPLPEEAKHLVMHAMLFI 63 ++++ Y NF+GN REA EFYAEVFG + P++MTFG+ PP+P +PLPE+AK+L+MH L I Sbjct: 1 MSVDAYLNFDGNCREAAEFYAEVFGLDKPKLMTFGDVPPNPEYPLPEDAKNLIMHTRLNI 60 Query: 64 DGSPVMFSDVFPGMPYVEGNNVNLTLTNDDAGKIKDWFNKLKEGGNVIMELQETFWSKCY 123 GS VMFSDVFPGMP+ GNN+ L +D K++ F++LK GGN+ MELQETFWSK Y Sbjct: 61 GGSNVMFSDVFPGMPFTVGNNITLAFVEEDEAKLRSAFDRLKVGGNIAMELQETFWSKLY 120 Query: 124 GSLKDKFGIIWQLSHDNGQ 142 G + DKFGI WQ + G+ Sbjct: 121 GQVTDKFGIHWQFNLGIGE 139 >B1HNS4.1 B1HNS4_LYSSC Protein phnB Length = 150 Score = 178 bits (451), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 81/139 (58%), Positives = 102/139 (73%) Query: 1 VKAVAINVYFNFNGNAREAVEFYAEVFGTEAPRIMTFGEAPPDPSHPLPEEAKHLVMHAM 60 V +A+ VY FNGN REAV FY + F T+ IMTFG++P +P +PLPEEAK LVMH Sbjct: 9 VLEMAVEVYLIFNGNCREAVAFYEDAFSTDKAEIMTFGDSPQNPDYPLPEEAKDLVMHTR 68 Query: 61 LFIDGSPVMFSDVFPGMPYVEGNNVNLTLTNDDAGKIKDWFNKLKEGGNVIMELQETFWS 120 L + GS +MFSD FPG P+ GNNV L + +DD +++ F+KLK+GG V+MELQETFWS Sbjct: 69 LSVFGSRIMFSDTFPGSPFTVGNNVTLAVVSDDEEQVRKAFDKLKDGGKVVMELQETFWS 128 Query: 121 KCYGSLKDKFGIIWQLSHD 139 K YGSL DKFG+ WQ SH+ Sbjct: 129 KWYGSLTDKFGVEWQFSHE 147 >A3IEW3.1 A3IEW3_9BACI Uncharacterized conserved protein, phnB family (Fragment) Length = 131 Score = 177 bits (449), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 79/128 (61%), Positives = 97/128 (75%) Query: 12 FNGNAREAVEFYAEVFGTEAPRIMTFGEAPPDPSHPLPEEAKHLVMHAMLFIDGSPVMFS 71 FNGN REAV FY + F T+ IMTFG++P +P +PLPEEAK LVMH L + GS +MFS Sbjct: 1 FNGNCREAVAFYEDAFSTDKAEIMTFGDSPQNPDYPLPEEAKDLVMHTRLSVFGSRIMFS 60 Query: 72 DVFPGMPYVEGNNVNLTLTNDDAGKIKDWFNKLKEGGNVIMELQETFWSKCYGSLKDKFG 131 D FPG PY GNNV L + +DD +++ F+KLK+GG V+MELQETFWSKCYGSL DKFG Sbjct: 61 DTFPGSPYAVGNNVTLAVVSDDEEQMRKAFDKLKDGGKVVMELQETFWSKCYGSLTDKFG 120 Query: 132 IIWQLSHD 139 + WQ SH+ Sbjct: 121 VEWQFSHE 128 >Q97D00.1 Q97D00_CLOAB Uncharacterized conserved protein, phnB family Length = 137 Score = 167 bits (424), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 77/136 (56%), Positives = 100/136 (73%) Query: 4 VAINVYFNFNGNAREAVEFYAEVFGTEAPRIMTFGEAPPDPSHPLPEEAKHLVMHAMLFI 63 +AI Y NF+GN REAVEFYAEVF T+ P+IM +G+ PP EE K+LV++A L + Sbjct: 1 MAIQAYINFDGNCREAVEFYAEVFETKKPKIMVYGDMPPKEGAKFNEETKNLVLNAALEV 60 Query: 64 DGSPVMFSDVFPGMPYVEGNNVNLTLTNDDAGKIKDWFNKLKEGGNVIMELQETFWSKCY 123 GS +MFSD+ PGM V GNN++L + +D ++K FN+LKE G V+MELQETFWSKCY Sbjct: 61 KGSTIMFSDIVPGMKLVVGNNISLVVIINDIDELKLIFNRLKEKGTVLMELQETFWSKCY 120 Query: 124 GSLKDKFGIIWQLSHD 139 G + DKFGI WQLS++ Sbjct: 121 GYVVDKFGIEWQLSYE 136 >C4VD15.1 C4VD15_ENTFA Protein PhnB Length = 140 Score = 144 bits (363), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 70/135 (51%), Positives = 91/135 (67%), Gaps = 2/135 (1%) Query: 6 INVYFNFNGNAREAVEFYAEVFGTEAPRIMTFGEAPPDPSHPLPEEAKHLVMHAMLFIDG 65 + +Y NF G A+EA+ FY +VF T+ +MTFGEAP DP HP+ +E K LVM+A + I+G Sbjct: 2 LELYINFKGEAKEAITFYEDVFDTKCQNLMTFGEAPEDPEHPINDEIKDLVMNASIIIEG 61 Query: 66 SPVMFSDV--FPGMPYVEGNNVNLTLTNDDAGKIKDWFNKLKEGGNVIMELQETFWSKCY 123 + VM SDV G EGNN++L ++ DD KI F +L EGG V M L ETFWSK Y Sbjct: 62 TYVMISDVPDMFGFEVTEGNNLSLVVSTDDDEKIDRLFKRLSEGGTVTMPLSETFWSKKY 121 Query: 124 GSLKDKFGIIWQLSH 138 GSLKD+FGI W ++ Sbjct: 122 GSLKDQFGIHWMFNY 136 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.137 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,033,167,946 Number of extensions: 44972722 Number of successful extensions: 98553 Number of sequences better than 1.0e-05: 649 Number of HSP's gapped: 98072 Number of HSP's successfully gapped: 649 Length of query: 147 Length of database: 3,060,696,203 Length adjustment: 110 Effective length of query: 37 Effective length of database: 2,024,384,553 Effective search space: 74902228461 Effective search space used: 74902228461 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 125 (52.8 bits)