BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0444.0012.p (290 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q65KX5.1 Q65KX5_BACLD Transmembrane lipoprotein 280 1e-73 Q5WIX2.1 Q5WIX2_BACSK Sugar ABC transporter permease 278 6e-73 Q2BFC3.1 Q2BFC3_9BACI Sugar ABC transporter permease 277 1e-72 B9MLU7.1 B9MLU7_ANATD Binding-protein-dependent transport system... 276 2e-72 B1DBB5.1 B1DBB5_9BACL Binding-protein-dependent transport system... 273 9e-72 >Q65KX5.1 Q65KX5_BACLD Transmembrane lipoprotein Length = 317 Score = 280 bits (715), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 137/287 (47%), Positives = 187/287 (65%), Gaps = 1/287 (0%) Query: 5 YLLAAPGMLYFVIFHYIPMSGLVLAFKEYSPFKGIINSPWVGLDNFRQFFGTSDFYXXXX 64 YLL PG+LYF+IF Y PM G+++AFK+YSP+ G S WVG + F+ FF DF+ Sbjct: 31 YLLLVPGVLYFLIFKYWPMWGVLIAFKDYSPYLGFWQSEWVGFEYFKDFFMNPDFFRLLR 90 Query: 65 XXXXXXXXXXVIYFPFTIIXXXXXXXXXXKVFKRVSQTIVYLPHFLSWVVIYGITISFFG 124 + FP +I ++KR QT +Y+PHF+SW ++ IT FF Sbjct: 91 NTLMLAALDLIFAFPAPLIVALLLNELRHAIYKRCIQTFIYVPHFVSWTIVVSITFVFFT 150 Query: 125 -PSGVINHYLTEWFGGSANFLVSNEWFRPLVIFQSIWKDAGWGTIIFLAALAGINPEQYE 183 +GV+N L G FL EWFRP+++ QSIWK+ GWGTI+FLAALA ++ EQYE Sbjct: 151 VDTGVMNKVLQSITGREIAFLSDPEWFRPMIVLQSIWKETGWGTILFLAALASVDQEQYE 210 Query: 184 AAKVDGANRFQNIWHITLPGIKSTIVILLLLRLGSSMDSNFMQIFLMTNSLNLDVSNVFD 243 AA +DGA R + +WHITLP I+STIV+LL+LR+GS ++ F QIFLMTNSLN V+ +FD Sbjct: 211 AAVMDGAGRLRRMWHITLPAIRSTIVVLLILRIGSFLNIGFEQIFLMTNSLNRSVAEIFD 270 Query: 244 LFVYHIGLEQFNYSFAITVGMFKSVASLILVLGANYAAKLLGEEGIF 290 +VY +G+ Q YS++ VG+FKSV +IL+ ANY AK ++G+F Sbjct: 271 TYVYVVGITQGAYSYSTAVGLFKSVVGIILIFCANYIAKKFDQDGLF 317 >Q5WIX2.1 Q5WIX2_BACSK Sugar ABC transporter permease Length = 319 Score = 278 bits (710), Expect = 6e-73, Method: Compositional matrix adjust. Identities = 132/287 (45%), Positives = 190/287 (66%), Gaps = 1/287 (0%) Query: 5 YLLAAPGMLYFVIFHYIPMSGLVLAFKEYSPFKGIINSPWVGLDNFRQFFGTSDFYXXXX 64 YL+ APG+LYF ++ YIPM GL++AF++Y P+ GI S WVGLD+FR+ F DF+ Sbjct: 33 YLMIAPGLLYFFVYKYIPMYGLIIAFQDYKPYLGITGSEWVGLDHFRRLFSDPDFWMIFK 92 Query: 65 XXXXXXXXXXVIYFPFTIIXXXXXXXXXXKVFKRVSQTIVYLPHFLSWVVIYGIT-ISFF 123 I FP II + ++R QT++Y+PHF+SWVVI I+ + Sbjct: 93 NTLVLFALQVCISFPVPIILALMLNELRSRAYQRSIQTMIYIPHFMSWVVIVSISYVMLT 152 Query: 124 GPSGVINHYLTEWFGGSANFLVSNEWFRPLVIFQSIWKDAGWGTIIFLAALAGINPEQYE 183 G++N+ L NFL++ EWFRP+ I Q IW++AGWGTIIFLAA+A ++P+ YE Sbjct: 153 LDGGIVNNILRALQFDQINFLLNPEWFRPMYILQIIWREAGWGTIIFLAAIAAVDPQLYE 212 Query: 184 AAKVDGANRFQNIWHITLPGIKSTIVILLLLRLGSSMDSNFMQIFLMTNSLNLDVSNVFD 243 AAK+DGANRF+ +WHITLP I+S I++LL+L++G ++ F ++L+ N+ N +V+ +FD Sbjct: 213 AAKMDGANRFRQMWHITLPAIRSVIIVLLILKIGDVLELGFEHVYLLLNASNREVAEIFD 272 Query: 244 LFVYHIGLEQFNYSFAITVGMFKSVASLILVLGANYAAKLLGEEGIF 290 +VY GL Q +S++ VG FK V LILV+ AN+ AK GEEGI+ Sbjct: 273 TYVYTAGLRQGQFSYSTAVGFFKGVVGLILVVMANWLAKRYGEEGIY 319 >Q2BFC3.1 Q2BFC3_9BACI Sugar ABC transporter permease Length = 325 Score = 277 bits (708), Expect = 1e-72, Method: Compositional matrix adjust. Identities = 147/286 (51%), Positives = 194/286 (67%), Gaps = 1/286 (0%) Query: 6 LLAAPGMLYFVIFHYIPMSGLVLAFKEYSPFKGIINSPWVGLDNFRQFFGTSDFYXXXXX 65 LL PG+L+F+++ YIPM+GL+LAFK+YSPF+G +SPW GL F F + Sbjct: 40 LLIMPGILFFLVYRYIPMAGLLLAFKDYSPFRGFADSPWAGLKYFYLIFEDKEVIRVIWN 99 Query: 66 XXXXXXXXXVIYFPFTIIXXXXXXXXXXKVFKRVSQTIVYLPHFLSWVVIYGITISFFGP 125 + FP +II + FKR Q+IVYLPHFLSWVV+ GIT+ F Sbjct: 100 TLQISMLQIIFAFPISIILALMLNELRNQFFKRFLQSIVYLPHFLSWVVVIGITVIFLRS 159 Query: 126 SGVINHYLTEWFGGSA-NFLVSNEWFRPLVIFQSIWKDAGWGTIIFLAALAGINPEQYEA 184 G++N++L+ FG A FL WF+PL++ Q IWK++GWGTIIFLAAL+GI+P YEA Sbjct: 160 EGLVNNFLSGAFGMEAIPFLTDPGWFKPLIVLQIIWKESGWGTIIFLAALSGISPHLYEA 219 Query: 185 AKVDGANRFQNIWHITLPGIKSTIVILLLLRLGSSMDSNFMQIFLMTNSLNLDVSNVFDL 244 A +DGA+R++ IWHITLP +KSTI+ILL+LRLG+ MDS F QIFLM N N++ NV D Sbjct: 220 AVMDGASRWRQIWHITLPALKSTIIILLILRLGTVMDSGFEQIFLMLNPFNMESGNVLDT 279 Query: 245 FVYHIGLEQFNYSFAITVGMFKSVASLILVLGANYAAKLLGEEGIF 290 FVY G++Q NYSFA VG+FK + L+LV+ AN AK GEEG++ Sbjct: 280 FVYFKGIQQANYSFATAVGLFKGIVGLVLVVLANRLAKRFGEEGLY 325 >B9MLU7.1 B9MLU7_ANATD Binding-protein-dependent transport systems inner membrane component Length = 321 Score = 276 bits (706), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 140/288 (48%), Positives = 185/288 (64%), Gaps = 1/288 (0%) Query: 4 FYLLAAPGMLYFVIFHYIPMSGLVLAFKEYSPFKGIINSPWVGLDNFRQFFGTSDFYXXX 63 Y+LA PG+LYF+IF YIPM G+V+AF++++PF G SPWVG ++F+ F DF Sbjct: 34 LYILALPGILYFIIFRYIPMFGIVVAFQDFNPFLGFWKSPWVGFEHFKTLFTDPDFPMLF 93 Query: 64 XXXXXXXXXXXVIYFPFTIIXXXXXXXXXXKVFKRVSQTIVYLPHFLSWVVIYGIT-ISF 122 + YFP II +V+KR+ QT VY+PHF+S VVI IT + Sbjct: 94 RNTLLISFYNILFYFPVPIILALLINEVRNQVYKRIVQTCVYVPHFVSMVVIASITYVLL 153 Query: 123 FGPSGVINHYLTEWFGGSANFLVSNEWFRPLVIFQSIWKDAGWGTIIFLAALAGINPEQY 182 +GVIN+ L G FL WFRPL+I QSIWK+AGWGTIIFLAAL+ ++P Y Sbjct: 154 SSEAGVINNILYSLTGKKIEFLTDPRWFRPLIIIQSIWKEAGWGTIIFLAALSNVDPTLY 213 Query: 183 EAAKVDGANRFQNIWHITLPGIKSTIVILLLLRLGSSMDSNFMQIFLMTNSLNLDVSNVF 242 EAA VDGA R+Q WHIT+P I ST++IL +LRLG +D+ F QIFLM N +N V+ VF Sbjct: 214 EAAIVDGATRWQQTWHITIPSIMSTVIILFILRLGHLLDTGFEQIFLMKNPINRSVAEVF 273 Query: 243 DLFVYHIGLEQFNYSFAITVGMFKSVASLILVLGANYAAKLLGEEGIF 290 D +VY +G+ Q YS++ VG+FKSV LIL+ +NY +K E +F Sbjct: 274 DTYVYQVGVTQGAYSYSTAVGLFKSVVGLILIQVSNYLSKKFTETSLF 321 >B1DBB5.1 B1DBB5_9BACL Binding-protein-dependent transport systems inner membrane component Length = 323 Score = 273 bits (699), Expect = 9e-72, Method: Compositional matrix adjust. Identities = 135/278 (48%), Positives = 187/278 (67%), Gaps = 1/278 (0%) Query: 14 YFVIFHYIPMSGLVLAFKEYSPFKGIINSPWVGLDNFRQFFGTSDFYXXXXXXXXXXXXX 73 YF+IF Y+PM G++LAFK Y PF G S WVGL++FR FF +F+ Sbjct: 46 YFIIFKYVPMWGVLLAFKNYQPFLGFWKSDWVGLEHFRVFFTNPEFFMLLRNTLILSLYN 105 Query: 74 XVIYFPFTIIXXXXXXXXXXKVFKRVSQTIVYLPHFLSWVVIYGITISFFGPSG-VINHY 132 V YFP II FKR+ QT++Y+PHF+S V++ +T F G VIN + Sbjct: 106 LVFYFPAPIILALLLNELRLAFFKRIVQTLIYIPHFISLVIVASLTYVFLTTEGGVINEF 165 Query: 133 LTEWFGGSANFLVSNEWFRPLVIFQSIWKDAGWGTIIFLAALAGINPEQYEAAKVDGANR 192 L ++ G +FL S +WFRP++I Q++WK+ G+GTIIFLAALAG++ EQYEAA DGANR Sbjct: 166 LFKYTGAKIDFLASPDWFRPMIIMQTVWKETGFGTIIFLAALAGVDVEQYEAAITDGANR 225 Query: 193 FQNIWHITLPGIKSTIVILLLLRLGSSMDSNFMQIFLMTNSLNLDVSNVFDLFVYHIGLE 252 ++ +WHITLP I+STI+ILL+LR+G +D+ F QIFLM N LN + + VFD +VY +G+ Sbjct: 226 WRQLWHITLPSIRSTIIILLILRMGDVLDNGFEQIFLMLNPLNRESAEVFDTYVYMMGIT 285 Query: 253 QFNYSFAITVGMFKSVASLILVLGANYAAKLLGEEGIF 290 Q +S++ VG+FK+V + LVLGAN+ AK G+ GI+ Sbjct: 286 QGAFSYSTAVGLFKAVIGVTLVLGANWLAKRFGQSGIY 323 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.330 0.145 0.464 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,616,858,783 Number of extensions: 63894275 Number of successful extensions: 196138 Number of sequences better than 1.0e-05: 3531 Number of HSP's gapped: 194005 Number of HSP's successfully gapped: 3531 Length of query: 290 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 156 Effective length of database: 1,798,280,193 Effective search space: 280531710108 Effective search space used: 280531710108 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)