BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0444.0010.p (718 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K5Z1.1 B3K5Z1_9BACI Heparinase II/III family protein 215 2e-53 B9XDB7.1 B9XDB7_9BACT Heparinase II/III family protein 206 7e-51 C0ACW6.1 C0ACW6_9BACT Putative uncharacterized protein 182 7e-44 C0D5E8.1 C0D5E8_9CLOT Putative uncharacterized protein 180 4e-43 Q02D12.1 Q02D12_SOLUE Heparinase II/III family protein 177 4e-42 >B3K5Z1.1 B3K5Z1_9BACI Heparinase II/III family protein Length = 603 Score = 215 bits (547), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 154/561 (27%), Positives = 253/561 (45%), Gaps = 43/561 (7%) Query: 50 YFDVRDVPG--------------LKAYAVNSYSQEEEAKQDIAEADRIAEGDIPLFKGKR 95 YF R PG +K Y + + +++ + + + + + Sbjct: 46 YFTCRRTPGWWMSGEDKQYTTDYVKQYCADELETVMKTAEEVVQQTFLFRFPWDMERSQI 105 Query: 96 VVFPKGTYEWNSWLYDSSQYQLHLTRFVYVKRLARAYALTGDEKYAKCFNDMMSHFIDDN 155 V +G+ W +++ L R Y L +AYA+TGDEKYA+ F + +ID N Sbjct: 106 PVIFEGSVNWCHIPDQDAEWAYMLNRHRYWVALGQAYAMTGDEKYAEAFCRQLEDWIDRN 165 Query: 156 PMPVDGTFRAEHSTWDPLSVGVRLFMLPEAFITFFSSPSFEPEVKLKLIKSFHEHGQYVR 215 P+P + T + TW P+ G+R +A SPS P + K + S HEHG Y+ Sbjct: 166 PIPEEPT--TDTLTWRPIEAGLRSANWIKALRYMIGSPSLTPLLLGKALISLHEHGDYLA 223 Query: 216 KYHATH---GNHVCMQLRGLIQTALLLPELKDSAEWLEYGMREMPGYIRQNVYPDGVQFE 272 + N ++ GL AL PE K SA W E G + R + DG+ +E Sbjct: 224 ASFTSWKHISNWGVLETCGLYHIALYFPEFKSSARWRELGEERLKETARLQIMVDGIHWE 283 Query: 273 GSPNYHLVVMRDLYELVALFHKLGIAA-GEYQEILENMFVVMMHLLAPDGQLVKFGDTDQ 331 SP YH V+ + V L + G+ G ++ + M ++ P+ + GD+D Sbjct: 284 QSPTYHHEVLACYLDCVHLANLNGLELDGLFRHKVREMAEASLYWAKPNHRQPMLGDSDD 343 Query: 332 QVASELRNVMSLGAYLYQRSDFKALGHERLPFSLIWRVGPEAVENYNQLKAVGPAETAAC 391 S++R++++ A+L++ + G+ + W G + +Y + P + Sbjct: 344 ---SDIRSILTYAAWLFEDPVLRFGGYALPDYDHAWLFGVSGIRDYEAMVPAEPPSLSRA 400 Query: 392 FPAGGYLTSRQGWKHTDMYMAMRAGVGVGGHAHSDALSLVLYAGGRELLADSGMGLFEWD 451 FP G+ T R GW +Y+ G GGH H+D L + L+A GR+LL D +G + + Sbjct: 401 FPHSGHYTMRTGWGEDALYLYFHCGPLGGGHGHADMLHMDLHAFGRDLLTD--LGRYNYS 458 Query: 452 KE---RKYVVSTRAHNTVVVDGQDQHVRSFHWNTPATA---ACRIWDFQTNEQYDYTFAS 505 RK + + AHNT VVDG W+ A CR + + ++DY + Sbjct: 459 DHTPLRKALKESSAHNTTVVDGIGFTGVKDTWSFDRIAGPLGCR---WISKPEFDYVEGA 515 Query: 506 HYGYTRYDDPVIHSRKVLFVKNSYWLIVDLFEAKGQHRYEQYFHL--PPGGVAYDCFTGE 563 H GY DP+ R+++F+K +WL++D QH + Q+FH PPG V + GE Sbjct: 516 HDGYLYGSDPLYPRRRIIFIKPYFWLLIDSLACLEQHTFTQHFHFPHPPGEVRME---GE 572 Query: 564 V----STQLEDANLLLVHPSA 580 +T +ANL ++ +A Sbjct: 573 SLICRTTTRGEANLFIIPVTA 593 >B9XDB7.1 B9XDB7_9BACT Heparinase II/III family protein Length = 717 Score = 206 bits (524), Expect = 7e-51, Method: Compositional matrix adjust. Identities = 170/597 (28%), Positives = 270/597 (45%), Gaps = 68/597 (11%) Query: 1 MSDEEFLRTMLDLDRPELAEAAAKLHEGNVSGARDAYLEVIASGNTGRYYFDVRDVPGLK 60 +SD EF +LDL RP+LAE + + + AR A E + + + D R G+ Sbjct: 73 LSDVEFF-ALLDLSRPDLAEVKKSVAGSDWTAARHALAEHMRTRPLPHWQIDPR---GVG 128 Query: 61 AYAVNSYSQEEEAKQDIAEADRIAE--GDIPLFKGKRVVFPKGTYEWNS-WLYDSSQYQL 117 A ++ E+A + + + I G+ + P + N+ W + ++++ Sbjct: 129 HDAKSNEPDAEKALKHLVNSQGIEWQFGETIDWAFNPTTQPDSKWPRNNEWTWQFNRHEQ 188 Query: 118 HLTRFVYVKRLARAYALTGDEKYAKCFNDMMSHFIDDNPMPVDGTFRAEHSTWDPLSVGV 177 L LA+AY TGDEKYA+ F ++ ++ D P+P+ S W + G+ Sbjct: 189 WLA-------LAKAYYATGDEKYAQEFVAELNSWVHDCPVPLAAVQNGPFSRWRTIEAGI 241 Query: 178 RL-FMLPEAFITFFSSPSFEPEVKLKLIKSFHEHGQYVRKYHATHGNHVCMQLRGLIQTA 236 R + PE F S+ SF+ + ++KSF +H +Y+ K+H T GN + M+ GL Sbjct: 242 RAGTVWPEIFPRVLSAKSFDDAAVVLMLKSFVDHAEYLMKFHTT-GNWLTMEANGLYHVG 300 Query: 237 LLLPELKDSAEWLEYGMREMPGYIRQNVYPDGVQFEGSPNYHLVVMRDLYELVALFHKLG 296 L PE KD+ W + + + VYPDG Q E +P YH + +R+ V L G Sbjct: 301 ALFPEFKDAQLWRDTAAERLDHELDVQVYPDGAQIELAPGYHGIALRNFSGPVELVSMTG 360 Query: 297 I-AAGEYQEILENMFVVMMHLLAPDGQLVKFGDTDQQVASELRNVMSLGA-YLYQRSDFK 354 +Y LE MF ++ + PD ++ D+ AS + M GA Y QR DF Sbjct: 361 FQPPKDYLPKLEKMFSYLLFSMQPDRRMPPLNDSG---ASSIVVGMRPGARYFPQREDF- 416 Query: 355 ALGHERLPFSLIWRVGPEAVENYNQLKAVGPAETAACFPAGGYLTSRQGWKHTDMYMAMR 414 +W N + P ET+ FP G R GW ++ M Sbjct: 417 -----------LWVA-------TNGKEGKPPMETSHVFPYAGQFIMRSGWDADARWLCMD 458 Query: 415 AGVGVGGHAHSDALSLVLYAGGRELLADSGMGLFEWDKERKYVVSTRAHNTVVVDGQDQH 474 G GH H D LS++L A G+ LL + G +++ + R+YV+S+RAHN V+VDG +Q Sbjct: 459 GGPFGYGHQHEDKLSVILTAYGQPLLVEGGAYIYDASEWRQYVLSSRAHNVVMVDGLEQA 518 Query: 475 VR-----SFHWNTPATAACRIWDFQTNEQYDYTFASHYGYTRYDD--------PVIHSRK 521 R +F P ++W ++N+ +D+ +RYD+ P+ +R Sbjct: 519 RRHEPKETFVVKNPLP---QVW--ESNDVFDHA------ASRYDEGWGAESLHPIRQTRH 567 Query: 522 VLFVKNSYWLIVDLFEAKG--QHRYEQYFHLPPGGVAYDCFTGEVSTQLEDANLLLV 576 V F+K ++I D E+ H E FHL D V+TQ NL ++ Sbjct: 568 VFFLKPDVFIIADELESMDGKPHDCEALFHLNAPDATVDGL--RVTTQNAGPNLTVM 622 >C0ACW6.1 C0ACW6_9BACT Putative uncharacterized protein Length = 1117 Score = 182 bits (463), Expect = 7e-44, Method: Compositional matrix adjust. Identities = 133/479 (27%), Positives = 209/479 (43%), Gaps = 50/479 (10%) Query: 112 SSQYQLHLTRFVYVKRLARAYALTGDEKYAKCFNDMMSHFIDDNPMPVDG-TFRAEHSTW 170 + ++Q L R + +A AY TG+EKY + F+ P P FR +STW Sbjct: 549 NQEWQYQLCRMSFWGDMANAYRATGNEKYPSAWVTQFRSFMAQCPPPTAAANFR--YSTW 606 Query: 171 DPLSVGVRLF-MLPEAFITFFSSPSFEPEVKLKLIKSFHEHGQYVRKYHATHGNHVCMQL 229 + G+R+ P AF F SPS E L + S +H +Y++K+ + GN + M++ Sbjct: 607 RTIESGIRMRDSWPRAFQAFVRSPSVSDEDLLLYMYSNLQHAEYLKKFPSDWGNWLTMEM 666 Query: 230 RGLIQTALLLPELKDSAEWLEYGMREMPGYIRQNVYPDGVQFEGSPNYHLVVMRDLYELV 289 GL L PE +D+ +W + + M I + PDG Q+E +P YH+V L E Sbjct: 667 CGLFTIGTLFPEFRDATDWRQTAVATMHASIARQFLPDGAQYELAPGYHIVA---LDENG 723 Query: 290 ALFHKLGIAAGEYQEI-------LENMFVVMMHLLAPDGQLVKFGDTDQQVASELRNVMS 342 +L A G EI +E + +++L+ P+ +F D+ + LR V + Sbjct: 724 MAIPRLAKATGRISEIPADYIASMEKGYDYLLYLMTPNRSEPRFNDS--WPITWLRPVFN 781 Query: 343 LGAYLY-QRSDFKALGHERLPFSLIWRVGPEAVENYNQLKAVGPAETAACFPAGGYLTSR 401 G + R D++ + + G E PA T+ FP GY R Sbjct: 782 NGLLFFPNRDDWRWIATD----------GAEGRP---------PAATSHAFPYAGYYVMR 822 Query: 402 QGWKHTDMYMAMRAGVGVGGHAHSDALSLVLYAGGRELLADSGMGLFEWDKERKYVVSTR 461 GW Y+A G+ H+H D L+LV + GRE+L D G G +E K R+Y Sbjct: 823 SGWGTDANYLAFDGGLPGYSHSHMDKLNLVFWVYGREILFDGGGGSYEDSKWRQYATDAY 882 Query: 462 AHNTVVVDGQDQHVRSFHWNTPATAACRIWDFQTNEQYDYTFASH---------YGYTRY 512 +HN ++VDG+ Q + N ++ +YD+ A+ + + Sbjct: 883 SHNVIMVDGKPQRHQLLDRNANVPREPNDVTWEATSEYDFVAATFDQAFSDVGMWSAKQP 942 Query: 513 DDPVIHSRKVLFVKNSYWLIVDLFEAKGQ-----HRYEQYFHLPPGGVAYDCFTGEVST 566 PV H+R+VLF K ++ D H YE +HL P D T ++T Sbjct: 943 PGPVTHTRRVLFAKPDLVIVADTLVPTDPADTTAHTYEARWHLIPTATITDPGTQTITT 1001 >C0D5E8.1 C0D5E8_9CLOT Putative uncharacterized protein Length = 714 Score = 180 bits (457), Expect = 4e-43, Method: Compositional matrix adjust. Identities = 131/465 (28%), Positives = 208/465 (44%), Gaps = 15/465 (3%) Query: 96 VVFPKGTYEWNSWLYDSSQYQLHLTRFVYVKRLARAYALTGDEKYAKCFNDMMSHFIDDN 155 V FP+G +W D ++ R Y L +AY +TGDEKYA+ F +++ +I Sbjct: 65 VEFPEGKVDWEYKPGDDPEFIYQFNRHRYFICLGQAYWITGDEKYARHFVELLMDWI--- 121 Query: 156 PMPVDGTFRAEHSTWDPLSVGVRLFMLPEAFITFFSSPSFEPEVKLKLIKSFHEHGQYVR 215 V T E +TW L VG+R +A F SP +V EH +++ Sbjct: 122 -TTVKRTPETEKTTWRILEVGIRGEYWVKAMQYFKDSPLVTDQVADAFYNCLIEHAEFII 180 Query: 216 KYHATH---GNHVCMQLRGLIQTALLLPELKDSAEWLEYGMREMPGYIRQNVYPDGVQFE 272 + H+ + N M+ GL + + +P+ + + + + R + DGVQ+E Sbjct: 181 RMHSPYRYMSNWGVMENHGLFEIGVAIPDEELRRRYTGIALEHLEVEARMEILRDGVQWE 240 Query: 273 GSPNYHLVVMRDLYELVALFHKLGIAAGEYQEILENMFVVMMHLLA---PDGQLVKFGDT 329 SP YH V+ +++ L + I + ILE + + LA PD GD+ Sbjct: 241 QSPMYHNEVLHCYEDVLILASRNDIDVPD--AILEAVHRMAWADLAWKKPDHHQFIMGDS 298 Query: 330 DQQVASELRNVMSLGAYLYQRSDFKALGHERLPFSLIWRVGPEAVENYNQLKAVGPAETA 389 D +++R+ +S+ A+L++ K G RL F +W +G E+ Y ++A+ P T+ Sbjct: 299 DD---TDIRDYISVAAFLFKDPMLKHGGFPRLDFESVWDLGIESAGLYEDMEAMEPDFTS 355 Query: 390 ACFPAGGYLTSRQGWKHTDMYMAMRAGVGVGGHAHSDALSLVLYAGGRELLADSGMGLFE 449 P G R GW+ + G GH HSD L + L A G ++L D+G + Sbjct: 356 VALPDSGNYYLRSGWEENANLLHFHCGTLGAGHGHSDKLHVDLVAFGEDILTDAGRYNYV 415 Query: 450 WDKERKYVVSTRAHNTVVVDGQDQHVRSFHWNTPATAACRIWDFQTNEQYDYTFASHYGY 509 R AHNT+ VDG+ V W F T E Y++ H GY Sbjct: 416 AGPGRFEFKDPTAHNTITVDGEFFTVCKDSWECSKLCQPVKQQFFTAEDYEFVQGGHLGY 475 Query: 510 TRYDDPVIHSRKVLFVKNSYWLIVDLFEAKGQHRYEQYFHLPPGG 554 V +RK+L++K ++I+D + G H YEQY+H G Sbjct: 476 MDMGQGVFVNRKILYIKPDIYVIMDEMYSGGAHTYEQYWHFSERG 520 >Q02D12.1 Q02D12_SOLUE Heparinase II/III family protein Length = 660 Score = 177 bits (448), Expect = 4e-42, Method: Compositional matrix adjust. Identities = 157/566 (27%), Positives = 238/566 (42%), Gaps = 59/566 (10%) Query: 1 MSDEEFLRTMLDLDRPELAEAAAKLHEGNVSGARDAYLEVIASGNTGRYYFDVRDVPGLK 60 MS+++ + LDL P L +AA G+ + AR A E YY PG K Sbjct: 13 MSEDDLFKD-LDLTTPGLEKAAV----GDRAEARHALAEYFRHRARPLYYI----APGEK 63 Query: 61 AYAVNSYSQEEEAKQDIAEADRIAEGDIPLFKGKRVVFPK------GTYEWNSWLYDSSQ 114 A A+ D+A +R + P+ T E S +++ Sbjct: 64 AIP-------RPARPDVARGERALRHEFESIGYPHTFGPEIDWHFDKTAEAGSKYPANNE 116 Query: 115 YQLHLTRFVYVKRLARAYALTGDEKYAKCFNDMMSHFIDDNPMPVDGTFRAEHSTWDPLS 174 + L R L+RAY T DEKYA+ F M+ + PMP D S W + Sbjct: 117 WTWQLNRHAEWSALSRAYRDTADEKYAREFVAQMTAWARGCPMPKDAG-NVPRSAWRTIE 175 Query: 175 VGVRLFML-PEAFITFFSSPSFEPEVKLKLIKSFHEHGQYVRKYHATHGNHVCMQLRGLI 233 G+R + PE + F +P+ + L ++++ +H ++ +H T GN + M+ GL Sbjct: 176 TGIRAAQVWPELWYRFLRAPAMTDDALLTFLRAYIDHAHHLMAFHTT-GNWLAMEGNGLY 234 Query: 234 QTALLLPELKDSAEWLEYGMREMPGYIRQNVYPDGVQFEGSPNYHLVVMRDLYELVALFH 293 +L PE + +A W E + + + VYPDGVQ E S YH V + + + + Sbjct: 235 YVGVLFPEFRAAAAWRETAGQWIYQEMNNQVYPDGVQVELSSGYHHVSLDNFLSVYKIAR 294 Query: 294 KLGIA-AGEYQEILENMFVVMMHLLAPDGQLVKFGDTDQQVASELRNVMSLGAYLYQRSD 352 G G++ + LE M+ + PDG+L G D R + Y +RSD Sbjct: 295 LNGTELPGDFLKRLEKMYDFDVFGAMPDGRLP--GVQDGNYYPVRRALAEAAEYFPERSD 352 Query: 353 FKALGHERLPFSLIWRVGPEAVENYNQLKAVGPAETAACFPAGGYLTSRQGWKHTDMYMA 412 F+ G + P +T+ F GY R GW Y+ Sbjct: 353 FQWYGS-------------------GGRQGRPPVDTSHAFRWAGYYVMRSGWDADARYLW 393 Query: 413 MRAGVGVGGHAHSDALSLVLYAGGRELLADSGMGLFEWDKERKYVVSTRAHNTVVVDGQD 472 G GH H D L +V+ A G+ LL D G +E K R Y + + +HN V+VDG+ Sbjct: 394 FDGGPFGYGHQHEDKLQIVVEAYGKLLLVDPGNYTYERSKWRSYFIDSPSHNVVLVDGEA 453 Query: 473 QHVRSFHWNT-----PATAACRIWDFQTNEQY-DYTFASHYGYTRYDDPVIHSRKVLFVK 526 Q R +T P A +W + Y + TF ++G V H+R VLFVK Sbjct: 454 QRRRGAPRDTYVIKEPLPA---VWKSAPDTDYVEATFDENFG-GEVKRNVTHTRAVLFVK 509 Query: 527 NSYWLIVDLFEAKG--QHRYEQYFHL 550 +W+++D AK H Y+ FH Sbjct: 510 PDFWVVLDTLTAKDGKPHMYDALFHF 535 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 4,573,663,333 Number of extensions: 202620623 Number of successful extensions: 417956 Number of sequences better than 1.0e-05: 165 Number of HSP's gapped: 417532 Number of HSP's successfully gapped: 179 Length of query: 718 Length of database: 3,060,696,203 Length adjustment: 143 Effective length of query: 575 Effective length of database: 1,713,491,058 Effective search space: 985257358350 Effective search space used: 985257358350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 135 (56.6 bits)