BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0444.0008.p (388 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C4CVD4.1 C4CVD4_9SPHI Putative uncharacterized protein 392 e-107 A4BEX7.1 A4BEX7_9GAMM Small-conductance mechanosensitive channel 262 5e-68 Q8DBS3.1 Q8DBS3_VIBVU Small-conductance mechanosensitive channel 233 2e-59 Q7MI47.1 Q7MI47_VIBVY Putative uncharacterized protein VV2670 232 5e-59 C0QAF6.1 C0QAF6_DESAH Putative mechanosensitive small-conductanc... 116 5e-24 >C4CVD4.1 C4CVD4_9SPHI Putative uncharacterized protein Length = 393 Score = 392 bits (1008), Expect = e-107, Method: Compositional matrix adjust. Identities = 204/380 (53%), Positives = 262/380 (68%), Gaps = 1/380 (0%) Query: 5 NKILFLIVTFGLIFLLPLIGSLSKWKGLPPGYGDFPAQKVEADPGFNLLYFSLACIVALV 64 K LI++F F+LP +GS KW GLPPGYG FPAQKV DP FN YF++ +VA+ Sbjct: 2 KKKTLLIISFLFFFILPFLGSYLKWGGLPPGYGLFPAQKVADDPPFNQTYFNVCSLVAIF 61 Query: 65 ITLILVFPRWFGFKKIKEEPAPKDGATPFPVWFWWSLPVLAVSWFLMWARVKLVISLEYY 124 I L+ P+ FGFKK + + A +PVWF +L V+ +SW LMW R K++ + + Sbjct: 62 ILTFLLLPQLFGFKKKPDAVESEKTAVGYPVWFKPALIVVLISWLLMWGRFKVLKPADNF 121 Query: 125 TFVPLWWSFILILDGLVYKRNKGASIISRKPHLMQLLAVVSCFSWFAFEFLNFFVIENWY 184 TFVPLWW FI +LDG+VYKRN G S++S KP+ +++LAV S FSWF FE+ NFFV+ENWY Sbjct: 122 TFVPLWWGFIFVLDGIVYKRNNGVSLMSAKPNTVKILAVTSAFSWFVFEYQNFFVLENWY 181 Query: 185 YPNDKVFSNFGNVFWFSLSYTTVLPAIFEWYLLLKTFGAFRNRYKNGPKFNVSRTFLILY 244 YPN+ + SNFGN+ W +SYTTVLPAIFEWY LL+T + +NRY GP S T + Sbjct: 182 YPNNNILSNFGNISWQLVSYTTVLPAIFEWYWLLRTVKSMKNRYSFGPAIRFSTTGQYIC 241 Query: 245 YVLGLILAFGMGYYPYVLFWVLWVALVPMLSAAMALAGFWTPFTPV-KNGDWSSVILIGL 303 +G I F MGY+P LFW+LWV+++PML AMA++GFWTPFT + K GDWS V+LI + Sbjct: 242 LGIGGISLFLMGYFPNELFWMLWVSMLPMLVPAMAISGFWTPFTEISKKGDWSFVVLIAI 301 Query: 304 ATLXXXXXXXXXXXXXXXXHDSLPTNPNYWKYSVPYLDKIHIFSEMPILGYFGYLFFGLN 363 +TL HD +PTNPNYWKYSVPYLDK H+FSEMPILGYFGYL FG Sbjct: 302 STLLNGFFWEFWNFGSEWFHDDMPTNPNYWKYSVPYLDKYHLFSEMPILGYFGYLLFGNA 361 Query: 364 CWIIWLIAAYIFKFNADIDV 383 CW++W+I A + F+A +D Sbjct: 362 CWLLWVIVARLVGFDASVDA 381 >A4BEX7.1 A4BEX7_9GAMM Small-conductance mechanosensitive channel Length = 384 Score = 262 bits (669), Expect = 5e-68, Method: Compositional matrix adjust. Identities = 143/360 (39%), Positives = 212/360 (58%), Gaps = 13/360 (3%) Query: 32 LPPGYGDFPAQKVEADPGFNLLYFSLACIVALVITLILVFPRWFGFKKIKEEPAPKDGAT 91 LPP +G FP +V PGFN +YF++ I L +T +++FP+ FGF+ PAPK T Sbjct: 30 LPPSFGTFPPVEVPGTPGFNWIYFTVLAIGGLAVTALILFPKLFGFQG--ATPAPKPTPT 87 Query: 92 PFPVWFWWSLPVLAVSWFLMWARVKLVISLEYYTFVPLWWSFILILDGLVYKRNKGASII 151 FP WFW + W+LMW+R L L Y++F P+WW FI++LDG+VY R G S+I Sbjct: 88 SFPWWFWVGAAFMCAFWWLMWSRSTLFGDLVYFSFSPMWWGFIIMLDGIVYARTGGKSLI 147 Query: 152 SRKPHLMQLLAVVSCFSWFAFEFLNFFVIENWYYPNDKV--FSNFGNVFWFSLSYTTVLP 209 +P L + + S F W+ FE+ ++FV+ NWYYPN S+ V F ++Y+TV P Sbjct: 148 RNRPTLFIISTLFSVFGWYYFEYFDYFVLSNWYYPNGVTSPLSHATVVAIFLVAYSTVWP 207 Query: 210 AIFEWYLLLKTFGAFRNRYKNGPKFNVSRTFLILYYVLGLILAFGMGYYPYVLFWVLWVA 269 AIFEWY LL ++ RY+NGP + ++LI + G+++ +PY +FW LW+ Sbjct: 208 AIFEWYTLLNSYPKLVARYQNGPNLPLQGSWLI---IGGIVVIAACVAWPYPVFWGLWIG 264 Query: 270 LVPMLSAAMALAGFWTPFTPVKNGDWSSVILIGLATLXXXXXXXXXXXXXXXXHD---SL 326 + M+ + G WTP V+ G+WS ILI +A+L + S Sbjct: 265 PLAMIVGQLMRLGIWTPMESVRQGNWSPAILIAVASLFNGFLWEFWNHGSEAYNPFNMSG 324 Query: 327 P---TNPNYWKYSVPYLDKIHIFSEMPILGYFGYLFFGLNCWIIWLIAAYIFKFNADIDV 383 P NPNYW Y VPY++ IH++SEMP+LGYFGYL FG+ W++++ A +F + +D+D+ Sbjct: 325 PIAQVNPNYWIYDVPYVNIIHLYSEMPLLGYFGYLPFGIFVWVMYIWAGKLFGWRSDLDL 384 >Q8DBS3.1 Q8DBS3_VIBVU Small-conductance mechanosensitive channel Length = 403 Score = 233 bits (594), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 139/386 (36%), Positives = 215/386 (55%), Gaps = 11/386 (2%) Query: 8 LFLIVTFGLIFLLPLIGSLSKW--KGLPPGYGDFPAQKVEADPGFNLLYFSLACIVALVI 65 L LI+ L+PL + K+ +PPG+ FP Q + +P FNL+YF L + + Sbjct: 3 LKLILPILATMLVPLAAAFFKYPDTHIPPGFLVFPPQFIVEEPSFNLIYFLLMLVAVVWA 62 Query: 66 TLILVFPRWFGFKKIKEEPAPKDGATPFPVWFWWSLPVLAVSWFLMWARVKLVISLEYYT 125 L+L +P GFK+ +P P P WF+ L + W+LMW+R + ++ YY Sbjct: 63 LLVLFWPEKVGFKR-NTQPMPSPTRANLPWWFYIGLGGTVLFWWLMWSRSEAMLPWVYYA 121 Query: 126 FVPLWWSFILILDGLVYKRNKGASIISRKPHLMQLLAVVSCFSWFAFEFLNFFVIENWYY 185 F PLWWSFI++LDG+VY+ N G S+ + KP L+ +A VS F W FE+ ++FV+ENWYY Sbjct: 122 FTPLWWSFIILLDGIVYRFNGGRSLCATKPGLLLFMAGVSFFGWMYFEYYDYFVLENWYY 181 Query: 186 PNDK--VFSNFGNVFWFSLSYTTVLPAIFEWYLLLKTFGAFRNRYKNGPKFNVSRTFLIL 243 PN + S + F SYTTV PAI +WY LL +F + R RY+ GP +++ +LI Sbjct: 182 PNAEKTSLSREMILLLFCFSYTTVTPAIMQWYTLLCSFPSIRKRYRFGPVWHLKPNWLIY 241 Query: 244 YYVLGLILAFGMGYYPYVLFWVLWVALVPMLSAAMALAGFWTPFTPV-KNGDWSSVILIG 302 GL F M ++P +LFW +W+ + +++ + G W PF + +G+W++ L+G Sbjct: 242 G---GLASIFLMVFFPKLLFWAVWIGPMAVMTGVLERYGIWNPFHGILTSGNWTAAALVG 298 Query: 303 LATLXXXXXXXXXXXXXXXXHDSLPTNPNYWKYSVPYLDK--IHIFSEMPILGYFGYLFF 360 +A+L L TNPNYW Y +P+++ + + +EMP+LGY GY+ F Sbjct: 299 MASLINGFFWEMWNFGSAHPDTQLITNPNYWVYDIPFVNLTWLPLPTEMPLLGYMGYVPF 358 Query: 361 GLNCWIIWLIAAYIFKFNADIDVTGD 386 G+ W + + + I N D+ D Sbjct: 359 GILVWQVLIWSGKIMGINTDLREVTD 384 >Q7MI47.1 Q7MI47_VIBVY Putative uncharacterized protein VV2670 Length = 403 Score = 232 bits (591), Expect = 5e-59, Method: Compositional matrix adjust. Identities = 139/386 (36%), Positives = 214/386 (55%), Gaps = 11/386 (2%) Query: 8 LFLIVTFGLIFLLPLIGSLSKW--KGLPPGYGDFPAQKVEADPGFNLLYFSLACIVALVI 65 L LI+ L+PL + K+ +PPG+ FP Q + +P FNL+YF L + + Sbjct: 3 LKLILPILATMLVPLAAAFFKYPDTHIPPGFLVFPPQFIVEEPSFNLIYFLLMLVAVVWA 62 Query: 66 TLILVFPRWFGFKKIKEEPAPKDGATPFPVWFWWSLPVLAVSWFLMWARVKLVISLEYYT 125 L+L +P GFK+ +P P P WF+ L + W+LMW+R + ++ YY Sbjct: 63 LLVLFWPEKVGFKR-NTQPMPSPTRANLPWWFYIGLGGTVLFWWLMWSRSEAMLPWVYYA 121 Query: 126 FVPLWWSFILILDGLVYKRNKGASIISRKPHLMQLLAVVSCFSWFAFEFLNFFVIENWYY 185 F PLWWSFI++LDG+VY+ N G S+ + KP L+ +A VS F W FE+ ++FV+ENWYY Sbjct: 122 FTPLWWSFIILLDGIVYRFNGGRSLCATKPGLLLFMAGVSFFGWMYFEYYDYFVLENWYY 181 Query: 186 PNDK--VFSNFGNVFWFSLSYTTVLPAIFEWYLLLKTFGAFRNRYKNGPKFNVSRTFLIL 243 PN + S + F SYTTV PAI +WY LL +F + R RY+ GP +++ +LI Sbjct: 182 PNAEKTSLSREMILLLFCFSYTTVTPAIMQWYTLLCSFPSIRKRYRFGPVWHLKPNWLIY 241 Query: 244 YYVLGLILAFGMGYYPYVLFWVLWVALVPMLSAAMALAGFWTPFTPV-KNGDWSSVILIG 302 GL F M ++P +LFW +W+ + +++ + G W PF + +G+W++ L+G Sbjct: 242 G---GLASIFLMVFFPKLLFWAVWIGPMAVMTGILERYGIWNPFHGILTSGNWTAAALVG 298 Query: 303 LATLXXXXXXXXXXXXXXXXHDSLPTNPNYWKYSVPYLDKI--HIFSEMPILGYFGYLFF 360 +A+L L TNPNYW Y +P+++ + +EMP+LGY GY+ F Sbjct: 299 MASLINGFFWEMWNFGSAHPDTQLITNPNYWVYDIPFVNLTWPPLPTEMPLLGYMGYVPF 358 Query: 361 GLNCWIIWLIAAYIFKFNADIDVTGD 386 G+ W + + + I N D+ D Sbjct: 359 GILVWQVLIWSGKIMGINTDLREVTD 384 >C0QAF6.1 C0QAF6_DESAH Putative mechanosensitive small-conductance channel MscC Length = 355 Score = 116 bits (290), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 103/361 (28%), Positives = 164/361 (45%), Gaps = 23/361 (6%) Query: 10 LIVTFGLIFLLPLIGSLSKWKGLPPGYGDFPAQKVEADPGFNLLYFSLACIVALVITLIL 69 +I+ GLI + PL+G L + + P Y FP + V G FSL +A ++ ++ Sbjct: 8 MIIPTGLILIFPLLGVLIDGQSVLP-YLVFPPEPVVTCHG----TFSLPVFMAFILLILT 62 Query: 70 VFPRWFGFKKIKEEPAPKDGATPFPVWFWWSLPVLAVSWFLMWARVKLVISLEYYTFVPL 129 + I A PFP W S LA W + W R + + +TF PL Sbjct: 63 ATLPFLKVGLIHTRKKSVAAAGPFPWWGVVSFSGLACFWIIAWTRFDWFVRFQPHTFFPL 122 Query: 130 WWSFILILDGLVYKRNKGASIISRKPHLMQLLAVVSCFSWFAFEFLNFFVIENWYYPNDK 189 W +I+ ++ LV++ + + ++L +VS W+ FE+LN FV NWYY + Sbjct: 123 WICWIVSVNALVHQSKNQCPLFTSPLKFLRLF-IVSALFWWVFEYLNRFV-GNWYYSASQ 180 Query: 190 VFSNFGNVFWFSLSYTTVLPAIFEWYLLLKTFGAFRNRYKNG---PKFNVSRTFLILYYV 246 + + +LS++TVLPA+ L TF F+N + + P N S+ F I + Sbjct: 181 -YRPWRYFLLATLSFSTVLPAVETTKAYLLTFDRFKNGFAHCRPVPYLN-SKPFAICLMI 238 Query: 247 LGLILAFGMGYYPYVLFWVLWVALVPMLSAAMALAGFWTPFTPVKNGDWSSVILIGLATL 306 + M +P LF ++WV + +L L + V GD++ V+ A L Sbjct: 239 CSCVCLCLMAMFPDALFPLVWVCPLLVLLGYRILFNRRHILSGVVPGDFTMVVAYASAAL 298 Query: 307 XXXXXXXXXXXXXXXXHDSLPTNPNYWKYSVPYLDKIHIFSEMPILGYFGYLFFGLNCWI 366 W+Y++PY+D +H+F EMP+LGY GYL FGL C + Sbjct: 299 VCGFFWEMFNMYSLAR----------WQYTIPYVDVLHLF-EMPLLGYAGYLPFGLECAV 347 Query: 367 I 367 + Sbjct: 348 V 348 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.331 0.147 0.508 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,578,698,557 Number of extensions: 113653170 Number of successful extensions: 340549 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 340515 Number of HSP's successfully gapped: 10 Length of query: 388 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 250 Effective length of database: 1,760,596,133 Effective search space: 440149033250 Effective search space used: 440149033250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)