BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0444.0001.p (358 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K3B2.1 B3K3B2_9BACI NADH:flavin oxidoreductase/NADH oxidase 403 e-110 A1U7C2.1 A1U7C2_MARAV NADH:flavin oxidoreductase/NADH oxidase 350 1e-94 C0TM48.1 C0TM48_9PSEU NADH:flavin oxidoreductase 345 5e-93 Q5WBM3.1 Q5WBM3_BACSK NADH-dependent flavin oxidoreductase 340 1e-91 Q4KI60.1 Q4KI60_PSEF5 NADH-dependent flavin oxidoreductase, Oye ... 338 5e-91 >B3K3B2.1 B3K3B2_9BACI NADH:flavin oxidoreductase/NADH oxidase Length = 374 Score = 403 bits (1036), Expect = e-110, Method: Compositional matrix adjust. Identities = 199/346 (57%), Positives = 238/346 (68%), Gaps = 4/346 (1%) Query: 16 RIVMSPVARAFSPGGVPGEDVTAYYRRRAENEVGLIITEGVVIDHPAASSEPRLPGIYGE 75 R+VM+P+ R FSP GVPG DV AYYRRRAEN VGLIITEG +I+HPAA+ P +P +GE Sbjct: 28 RVVMAPMTRGFSPNGVPGPDVAAYYRRRAENGVGLIITEGTLINHPAATDNPNVPNFHGE 87 Query: 76 AAMDGWREVVRQVHEAGGTIFPQLVHLGMIRPMGAEPFPEAPSIGPSGLDLEGRQVTGPM 135 AA++GW EVVRQVHEAGG I PQ+ H+GM RP+GAEP PEA IGPSGLDL+G VT PM Sbjct: 88 AALNGWAEVVRQVHEAGGKIMPQIWHIGMTRPLGAEPNPEALPIGPSGLDLDGNVVTKPM 147 Query: 136 TEEEIXXXXXXXXXXXXXXXXXXXDGVELHGAHGFLIDQFFWEKTNQRTDRYGGDFLKRT 195 T++EI DGVE+HGAHG+LIDQF WE+TN+RTD YGGD LKR Sbjct: 148 TKDEIEQVVQAYADAAANAKRLGFDGVEIHGAHGYLIDQFLWERTNRRTDEYGGDMLKRA 207 Query: 196 XXXXXXXXXXXXXXGPDYPVSMRLSQWKMGDYAAKLVGTPEQLEQFLNVLVKAGVDIFHI 255 GPD+PV++RLSQWK Y AKL TPE+L Q L L AGVDIFH Sbjct: 208 RFAIEVVKAVRQAVGPDFPVAIRLSQWKTNQYEAKLADTPEKLGQLLTALSDAGVDIFHC 267 Query: 256 STRRFWDPEFEGSELSLAGWVKKLSGKTTITVGSVGMEDE----PSEDGEAGRHGXXXXX 311 STRRFW+PEFEGS+L+ AGW KKL+GKT ITVGSVG+++ ++ AG+ Sbjct: 268 STRRFWEPEFEGSDLNFAGWAKKLTGKTVITVGSVGLDEVFTSLFTQGKGAGQRDLEELL 327 Query: 312 XXXXXXXFDLIAVGRALLGDPAWAKKIREGRVDEIKAFTPEATAVL 357 FDL+AVGRALL DP WA K+R+GR DE+ FTPE+ L Sbjct: 328 NRLEENEFDLVAVGRALLADPEWAAKVRDGRFDELAPFTPESLQTL 373 >A1U7C2.1 A1U7C2_MARAV NADH:flavin oxidoreductase/NADH oxidase Length = 370 Score = 350 bits (897), Expect = 1e-94, Method: Compositional matrix adjust. Identities = 177/359 (49%), Positives = 226/359 (62%), Gaps = 6/359 (1%) Query: 6 FQLLLKQQIRRIVMSPVARAFSPGGVPGEDVTAYYRRRAENEVGLIITEGVVIDHPAASS 65 F+L + R+ M+P+ R FSP GVPG+DV YYRRRAE VGLIITEG V++H AA+ Sbjct: 12 FELHNLKLRNRVAMAPMTRNFSPKGVPGQDVVDYYRRRAEAGVGLIITEGTVVNHAAANG 71 Query: 66 EPRLPGIYGEAAMDGWREVVRQVHEAGGTIFPQLVHLGMIRPMGAEPFPEAPSIGPSGLD 125 P +P YG+ A+ GW++VV VHEAGG IFPQL H+G +R G EP P P PSGL Sbjct: 72 YPNVPAFYGDEALAGWKKVVDAVHEAGGAIFPQLWHVGAVRKEGIEPDPSVPGYSPSGLF 131 Query: 126 LEGRQVTGPMTEEEIXXXXXXXXXXXXXXXXXXXDGVELHGAHGFLIDQFFWEKTNQRTD 185 G+ MT+E+I DGVE+HGAHG+L+DQF WE TNQR D Sbjct: 132 APGKPNGKAMTKEDIQDVIKAFGDAAQDAKALGFDGVEIHGAHGYLLDQFLWEGTNQRDD 191 Query: 186 RYGGDFLKRTXXXXXXXXXXXXXXGPDYPVSMRLSQWKMGDYAAKLVGTPEQLEQFLNVL 245 YGG R GPDYP+ +R SQWK DY AKLV +PE+LE+FL L Sbjct: 192 EYGGSLENRLRFVVEIVEEVRHRVGPDYPIMLRFSQWKQQDYEAKLVNSPEELERFLKPL 251 Query: 246 VKAGVDIFHISTRRFWDPEFEGSELSLAGWVKKLSGKTTITVGSVGMEDE------PSED 299 V+AGVDIFH STRRFW+PEFEGS L+LAGW +K++GK T++VGSVG+ ++ S+ Sbjct: 252 VEAGVDIFHASTRRFWEPEFEGSNLNLAGWTQKITGKPTMSVGSVGLTEDFISGTFASKK 311 Query: 300 GEAGRHGXXXXXXXXXXXXFDLIAVGRALLGDPAWAKKIREGRVDEIKAFTPEATAVLF 358 + G FDLIAVGRALL DP W K++EGR++E++AF ++ A L+ Sbjct: 312 EAVEQSGIDELVERMNNGEFDLIAVGRALLQDPEWLVKVKEGRLNEVEAFAKKSLAKLY 370 >C0TM48.1 C0TM48_9PSEU NADH:flavin oxidoreductase Length = 374 Score = 345 bits (884), Expect = 5e-93, Method: Compositional matrix adjust. Identities = 175/347 (50%), Positives = 218/347 (62%), Gaps = 5/347 (1%) Query: 16 RIVMSPVARAFSPGGVPGEDVTAYYRRRAENEVGLIITEGVVIDHPAASSEPRLPGIYGE 75 R+ M+P+ RAFSP G PG DV +YY RRA VGLI+TEG ++H +A R+P +GE Sbjct: 27 RVAMAPMTRAFSPDGTPGADVASYYARRAAGGVGLIVTEGTYVNHRSAGLSDRIPRFHGE 86 Query: 76 AAMDGWREVVRQVHEAGGTIFPQLVHLGMIRPMGAEPFPEAPSIGPSGLDLEGRQVTGPM 135 A + GW VV QVH AGGTI PQL H+GM RP G+ P P+APSIGPSG+ + G M Sbjct: 87 AQLAGWANVVEQVHAAGGTIVPQLWHVGMARPAGSAPNPDAPSIGPSGVGIGGTADGDTM 146 Query: 136 TEEEIXXXXXXXXXXXXXXXXXXXDGVELHGAHGFLIDQFFWEKTNQRTDRYGGDFLKRT 195 T+ +I DG+ELHGAHG+LIDQF W TN+RTD YGGD + RT Sbjct: 147 TQRDIDDVVAAFATAAADAERIGFDGIELHGAHGYLIDQFLWAGTNRRTDAYGGDPVART 206 Query: 196 XXXXXXXXXXXXXXGPDYPVSMRLSQWKMGDYAAKLVGTPEQLEQFLNVLVKAGVDIFHI 255 PD+P+ +R+SQWKMGDYAA++ TP++LE L L AGVD+FH Sbjct: 207 RFAAEVIAAVRAAVSPDFPLILRMSQWKMGDYAARIADTPQELEAILAPLAAAGVDVFHA 266 Query: 256 STRRFWDPEFEG-SELSLAGWVKKLSGKTTITVGSVGMEDEPSE----DGEAGRHGXXXX 310 STRR+WDPEF+G +L+LAGWVKKL+GK TI+VGSVG++ + S+ D A R Sbjct: 267 STRRYWDPEFDGEGDLNLAGWVKKLTGKATISVGSVGLDADVSKFFGGDPSAARADIDAL 326 Query: 311 XXXXXXXXFDLIAVGRALLGDPAWAKKIREGRVDEIKAFTPEATAVL 357 FDLIAVGRALL DP W K+ GR DEI FT +A L Sbjct: 327 LDRLERDEFDLIAVGRALLADPEWVAKVVSGRADEITPFTKDAATTL 373 >Q5WBM3.1 Q5WBM3_BACSK NADH-dependent flavin oxidoreductase Length = 346 Score = 340 bits (872), Expect = 1e-91, Method: Compositional matrix adjust. Identities = 172/348 (49%), Positives = 216/348 (62%), Gaps = 28/348 (8%) Query: 15 RRIVMSPVARAFSPGGVPGEDVTAYYRRRAENEVGLIITEGVVIDHPAASSEPRLPGIYG 74 RIVM+P+ R FSPGGVPG DV AYYRRRAEN VGLI+TEG VI+HP +S++ +P IYG Sbjct: 23 NRIVMAPMTRQFSPGGVPGPDVAAYYRRRAENGVGLIVTEGTVIEHPDSSNQENVPHIYG 82 Query: 75 EAAMDGWREVVRQVHEAGGTIFPQLVHLGMIRPMGAEPFPEAPSIGPSGLDLEGRQVTGP 134 + A++GW VV +VH+AGG I PQ+ H+G R G Sbjct: 83 DKALNGWANVVAEVHKAGGRIIPQIWHMG------------------------ARGHVGD 118 Query: 135 MTEEEIXXXXXXXXXXXXXXXXXXXDGVELHGAHGFLIDQFFWEKTNQRTDRYGGDFLKR 194 TE ++ DG+ELHGAH +LIDQFFW++TN RTDRYGGD ++R Sbjct: 119 YTENDVAAIVDAFAQAAAEAKRIGFDGIELHGAHQYLIDQFFWDQTNPRTDRYGGDMVER 178 Query: 195 TXXXXXXXXXXXXXXGPDYPVSMRLSQWKMGDYAAKLVGTPEQLEQFLNVLVKAGVDIFH 254 T GPD+P+ +R SQWK DY AKL T +LEQFL L AGVD+FH Sbjct: 179 TRFAVEVIEACRRAVGPDFPIVLRFSQWKSSDYTAKLATTAAELEQFLAPLTDAGVDVFH 238 Query: 255 ISTRRFWDPEFEGSELSLAGWVKKLSGKTTITVGSVGMEDEPS----EDGEAGRHGXXXX 310 STRRFW+PEFEGS+L+LAGW KKL+GK TITVGSVG++ E + E AG Sbjct: 239 CSTRRFWEPEFEGSDLNLAGWTKKLTGKPTITVGSVGLDSEFTKLFAEGKGAGVSNINGL 298 Query: 311 XXXXXXXXFDLIAVGRALLGDPAWAKKIREGRVDEIKAFTPEATAVLF 358 FDL+A+GRALL DPAWA KIR+GR++E+ F+P+A L+ Sbjct: 299 IEKLEQGEFDLVAIGRALLADPAWASKIRDGRINELTPFSPDAVKTLY 346 >Q4KI60.1 Q4KI60_PSEF5 NADH-dependent flavin oxidoreductase, Oye family Length = 367 Score = 338 bits (867), Expect = 5e-91, Method: Compositional matrix adjust. Identities = 175/357 (49%), Positives = 220/357 (61%), Gaps = 5/357 (1%) Query: 6 FQLLLKQQIRRIVMSPVARAFSPGGVPGEDVTAYYRRRAENEVGLIITEGVVIDHPAASS 65 FQL + R+VM+P+ R+FSPGGVP V YYRRRA VGLIITEG + H AA+ Sbjct: 10 FQLGALELPTRVVMAPMTRSFSPGGVPNSKVIEYYRRRAAAGVGLIITEGTTVGHQAANG 69 Query: 66 EPRLPGIYGEAAMDGWREVVRQVHEAGGTIFPQLVHLGMIRPMGAEPFPEAPSIGPSGLD 125 P +P +GEAA+ GW++VV VH GG I PQL H+G +R +G EP P GP+ Sbjct: 70 YPNVPQFHGEAALAGWKKVVDAVHAEGGKIVPQLWHVGNVRRLGTEPDASVPGYGPTEKL 129 Query: 126 LEGRQVTGPMTEEEIXXXXXXXXXXXXXXXXXXXDGVELHGAHGFLIDQFFWEKTNQRTD 185 +G+ + MT ++I DGVE+HGAHG+L+DQFFWE TNQRTD Sbjct: 130 KDGKVLVHGMTHQDIQEVIAAFAQAAKDAQSIGMDGVEIHGAHGYLVDQFFWEGTNQRTD 189 Query: 186 RYGGDFLKRTXXXXXXXXXXXXXXGPDYPVSMRLSQWKMGDYAAKLVGTPEQLEQFLNVL 245 YGGD +R+ GPD+P+ +R SQWK DY+A+LV TPE LE FL L Sbjct: 190 EYGGDLAQRSRFAIELIQAVRAAVGPDFPIILRFSQWKQQDYSARLVQTPEALEAFLKPL 249 Query: 246 VKAGVDIFHISTRRFWDPEFEGSELSLAGWVKKLSGKTTITVGSVGMEDE-----PSEDG 300 AGVDIFH STRRFW+PEFEGS+L+LAGW +KL+GK TITVGSVG++ E + D Sbjct: 250 ADAGVDIFHCSTRRFWEPEFEGSDLNLAGWTRKLTGKPTITVGSVGLDGEFLQFMVNTDK 309 Query: 301 EAGRHGXXXXXXXXXXXXFDLIAVGRALLGDPAWAKKIREGRVDEIKAFTPEATAVL 357 A FDL+AVGRALL DP WA+K+REGR +I F+ +A L Sbjct: 310 VAQPASLENLLQRLNNDEFDLVAVGRALLVDPDWAQKVREGREQDILPFSRDALTTL 366 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.139 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,024,211,266 Number of extensions: 79459265 Number of successful extensions: 177208 Number of sequences better than 1.0e-05: 3211 Number of HSP's gapped: 172346 Number of HSP's successfully gapped: 3220 Length of query: 358 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 221 Effective length of database: 1,770,017,148 Effective search space: 391173789708 Effective search space used: 391173789708 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)