BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0443.0039.p (223 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A7GUK5.1 A7GUK5_BACCN SNARE associated Golgi protein 169 2e-40 A0RKL7.1 A0RKL7_BACAH DedA family protein 168 4e-40 C2XIV4.1 C2XIV4_BACCE DedA 167 5e-40 A9VP35.1 A9VP35_BACWK SNARE associated Golgi protein 167 5e-40 C2RFE8.1 C2RFE8_BACCE DedA 167 8e-40 >A7GUK5.1 A7GUK5_BACCN SNARE associated Golgi protein Length = 204 Score = 169 bits (428), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 80/163 (49%), Positives = 111/163 (68%), Gaps = 5/163 (3%) Query: 4 LKWIQELFAXXXXXXXXXXXXXXXXXXPFPGETTMAFAGFLSYTGKLDFFVLIAVAFAGT 63 ++WI ELF PFPGE T+A+AG+L++TG L+ FVLI ++F GT Sbjct: 1 MEWIHELFQQYGYYVVLVGLLLEYIALPFPGEPTLAYAGYLAHTGDLNLFVLIILSFIGT 60 Query: 64 TVGMTVTYFIGLKAGLPFIQRYGKWFLFSETKLEKTQHWFDRYGSILVSIGYFIPGVRHF 123 ++GMT+ YF+G K G+PF+Q+YGK+ ++ K++ T+ WFD+YG L+ IG+FIPGVRHF Sbjct: 61 SIGMTIQYFLGNKLGMPFVQKYGKYVFLTQRKIDLTRMWFDKYGYFLIFIGFFIPGVRHF 120 Query: 124 TGYFSGITALPFRKFALYAYSGALLWVVLFLGIGKIFGPQWMG 166 TGYF GI LPFR+FA+ Y+GAL WV+ FL G W+G Sbjct: 121 TGYFGGIINLPFRRFAITIYAGALFWVLFFL-----LGGYWLG 158 >A0RKL7.1 A0RKL7_BACAH DedA family protein Length = 228 Score = 168 bits (425), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 87/206 (42%), Positives = 122/206 (59%) Query: 2 EMLKWIQELFAXXXXXXXXXXXXXXXXXXPFPGETTMAFAGFLSYTGKLDFFVLIAVAFA 61 + ++WI ELF PFPGE T+A+AGFL++ G L +LI ++F Sbjct: 23 DDMEWIHELFQQYGYYVVLVGLLLEYIALPFPGEPTLAYAGFLAHKGDLSLPILIILSFI 82 Query: 62 GTTVGMTVTYFIGLKAGLPFIQRYGKWFLFSETKLEKTQHWFDRYGSILVSIGYFIPGVR 121 GT+VGMT+ YF+G K G+PFIQ+YGK+ ++ K++ T+ WFD+YG L+ IG+FIPGVR Sbjct: 83 GTSVGMTIQYFLGNKLGMPFIQKYGKYVFLTQRKIDLTRMWFDKYGYFLIFIGFFIPGVR 142 Query: 122 HFTGYFSGITALPFRKFALYAYSGALLWVVLFLGIGKIFGPQWMGIFHLVEKNXXXXXXX 181 HFTGYF+GI LPFR+FA+ YSGAL WV FL G G IF ++E + Sbjct: 143 HFTGYFAGIINLPFRRFAITIYSGALFWVSFFLIGGYWLGGNLHDIFGVLEGHIGKIIFG 202 Query: 182 XXXXXXXXXLYRYRHKIGSRLLRRKT 207 R+R ++ LL+ + Sbjct: 203 VIVIVAITLGVRFRKQLKRALLQNAS 228 >C2XIV4.1 C2XIV4_BACCE DedA Length = 204 Score = 167 bits (424), Expect = 5e-40, Method: Compositional matrix adjust. Identities = 88/204 (43%), Positives = 121/204 (59%) Query: 4 LKWIQELFAXXXXXXXXXXXXXXXXXXPFPGETTMAFAGFLSYTGKLDFFVLIAVAFAGT 63 ++WI ELF PFPGE T+A+AGFL++ G L +LI ++F GT Sbjct: 1 MEWIHELFQQYGYYVVLVGLLLEYIALPFPGEPTLAYAGFLAHKGDLSLPILIILSFIGT 60 Query: 64 TVGMTVTYFIGLKAGLPFIQRYGKWFLFSETKLEKTQHWFDRYGSILVSIGYFIPGVRHF 123 +VGMT+ YF+G K G+PFIQRYGK+ ++ K++ T+ WFD+YG L+ IG+FIPGVRHF Sbjct: 61 SVGMTIQYFLGNKLGMPFIQRYGKYVFLTQRKIDLTRMWFDKYGYFLIFIGFFIPGVRHF 120 Query: 124 TGYFSGITALPFRKFALYAYSGALLWVVLFLGIGKIFGPQWMGIFHLVEKNXXXXXXXXX 183 TGYF+GI LPFR+FA+ YSGAL WV FL G G IF ++E + Sbjct: 121 TGYFAGIINLPFRRFAITIYSGALFWVSFFLIGGYWLGGNLHDIFGVLEGHIGKIIFGVI 180 Query: 184 XXXXXXXLYRYRHKIGSRLLRRKT 207 R+R ++ LL+ + Sbjct: 181 VIVAVTLGIRFRKQLKRVLLQNAS 204 >A9VP35.1 A9VP35_BACWK SNARE associated Golgi protein Length = 204 Score = 167 bits (424), Expect = 5e-40, Method: Compositional matrix adjust. Identities = 83/171 (48%), Positives = 111/171 (64%) Query: 4 LKWIQELFAXXXXXXXXXXXXXXXXXXPFPGETTMAFAGFLSYTGKLDFFVLIAVAFAGT 63 ++W+ ELF PFPGE T+A+AGFL+ G L +LI ++F GT Sbjct: 1 MEWVHELFQQYGYYVVLVGLLLEYIALPFPGEPTLAYAGFLAQKGDLSLPILIILSFIGT 60 Query: 64 TVGMTVTYFIGLKAGLPFIQRYGKWFLFSETKLEKTQHWFDRYGSILVSIGYFIPGVRHF 123 +VGMT+ YF+G K G+PF+Q+YGK+ ++ K++ T+ WFD+YG L+ IG+FIPGVRHF Sbjct: 61 SVGMTIQYFLGNKLGMPFVQKYGKYVFLTQRKIDLTRMWFDKYGYFLIFIGFFIPGVRHF 120 Query: 124 TGYFSGITALPFRKFALYAYSGALLWVVLFLGIGKIFGPQWMGIFHLVEKN 174 TGYF+GI LPFR+FA+ YSGAL WV FL G G IF L+E N Sbjct: 121 TGYFAGIINLPFRRFAITIYSGALFWVSFFLIGGYWLGGNLHDIFGLLEDN 171 >C2RFE8.1 C2RFE8_BACCE DedA Length = 204 Score = 167 bits (422), Expect = 8e-40, Method: Compositional matrix adjust. Identities = 87/204 (42%), Positives = 121/204 (59%) Query: 4 LKWIQELFAXXXXXXXXXXXXXXXXXXPFPGETTMAFAGFLSYTGKLDFFVLIAVAFAGT 63 ++WI ELF PFPGE T+A+AGFL++ G L +LI ++F GT Sbjct: 1 MEWIHELFQQYGYYVVLVGLLLEYIALPFPGEPTLAYAGFLAHKGDLSLPILIILSFIGT 60 Query: 64 TVGMTVTYFIGLKAGLPFIQRYGKWFLFSETKLEKTQHWFDRYGSILVSIGYFIPGVRHF 123 +VGMT+ YF+G K G+PFIQ+YGK+ ++ K++ T+ WFD+YG L+ IG+FIPGVRHF Sbjct: 61 SVGMTIQYFLGNKLGMPFIQKYGKYVFLTQRKIDLTRMWFDKYGYFLIFIGFFIPGVRHF 120 Query: 124 TGYFSGITALPFRKFALYAYSGALLWVVLFLGIGKIFGPQWMGIFHLVEKNXXXXXXXXX 183 TGYF+GI LPFR+FA+ YSGAL WV FL G G IF ++E + Sbjct: 121 TGYFAGIINLPFRRFAITIYSGALFWVAFFLIGGYWLGGNLHDIFGVLEGHIGKIIFGVI 180 Query: 184 XXXXXXXLYRYRHKIGSRLLRRKT 207 R+R ++ LL+ + Sbjct: 181 VIVAVTLGIRFRKQLKRVLLQNAS 204 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.330 0.146 0.464 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,127,342,505 Number of extensions: 42666296 Number of successful extensions: 117785 Number of sequences better than 1.0e-05: 1786 Number of HSP's gapped: 117662 Number of HSP's successfully gapped: 1786 Length of query: 223 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 92 Effective length of database: 1,826,543,238 Effective search space: 168041977896 Effective search space used: 168041977896 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 128 (53.9 bits)