BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0443.0036.p (480 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D8X7.1 B1D8X7_9BACL Drug resistance transporter, EmrB/QacA sub... 593 e-167 B3KDK4.1 B3KDK4_9BACI Drug resistance transporter, EmrB/QacA sub... 589 e-166 B1DHG1.1 B1DHG1_9BACL Drug resistance transporter, EmrB/QacA sub... 581 e-164 C1P747.1 C1P747_BACCO Drug resistance transporter, EmrB/QacA sub... 494 e-138 C1PAM8.1 C1PAM8_BACCO Drug resistance transporter, EmrB/QacA sub... 484 e-135 >B1D8X7.1 B1D8X7_9BACL Drug resistance transporter, EmrB/QacA subfamily Length = 505 Score = 593 bits (1528), Expect = e-167, Method: Compositional matrix adjust. Identities = 281/460 (61%), Positives = 353/460 (76%) Query: 1 VALLNQTLMNVALPKMMEDLKIAANTAQWLTTGFMLVNGVLIPISAYLVEKFTTRQLFTT 60 VALL+QTL+NVALP +M DLK++ANT QWL+TG++LVNGVLIPISAYL+ +F+TR LF Sbjct: 23 VALLSQTLLNVALPSIMTDLKVSANTVQWLSTGYLLVNGVLIPISAYLISRFSTRSLFVV 82 Query: 61 AMMLFSIGTLVCAIGTGFEMIMVGRVIQAVGAGILMPLMNIVFLRIFPIEERGKAMGLMA 120 A LF++GT+V A+ FE ++ GR+IQA GAGILMPLM+I+FL IFPIE+RGKAMG+M Sbjct: 83 ATSLFTVGTIVSALAPSFETLLTGRIIQAAGAGILMPLMSIIFLTIFPIEQRGKAMGMMG 142 Query: 121 VAMIFAPAVGPTLSGWVVQNYSWRVLFYIVLPLAVFSTLLGMKTMQNVGKLTSPRLDKMG 180 +AMIFAPAVGPTLSGWVV++Y WRVLFYIVLPL++F + G +M+NV + +LD Sbjct: 143 IAMIFAPAVGPTLSGWVVEHYDWRVLFYIVLPLSLFGLIYGAISMKNVTQTAKSKLDIFA 202 Query: 181 VVLSTLAFGGLLYGFSDAGTDGWNSATVYACLILGVVALALFVWRELKTEKPLLEFRIFR 240 V+LSTL FGGLLYGFSDAGTDGWN+ TVY LI+GV++L FVW +L +KP+LE R+F+ Sbjct: 203 VILSTLGFGGLLYGFSDAGTDGWNNVTVYTSLIVGVISLIGFVWYQLVVKKPILEMRVFK 262 Query: 241 YNMYSLTTVINIIVTMALYSGMILLPIYLQTIRGFTPMESGLMLLPGAILMGVMSPITGI 300 + MYSLTTVIN+I+TMA++SGMILLPIYLQ+I GFTP++SGL+LLPGAILMG+MSPITG+ Sbjct: 263 FGMYSLTTVINVIITMAMFSGMILLPIYLQSIMGFTPLKSGLLLLPGAILMGIMSPITGM 322 Query: 301 IFDKIGARWLSVIGLIITTITTWEFSQXXXXXXXXXXXXXXXARMFGMSMLMMPIVTAGL 360 IFDK+GARWL+V GL+IT +++EFSQ RMFGMSMLMMPI TAG+ Sbjct: 323 IFDKVGARWLAVTGLVITVYSSYEFSQLTDHTTYGYLITMYTLRMFGMSMLMMPIQTAGM 382 Query: 361 NQLPQRLASHGTAMSNTLRTVGGALGMALFVSLMTNRTKSDITEAVMSGSVSQTDKAAML 420 NQLPQRL +HG+AMS TLR V GALG A V+LMTN+ S+ V + + DKAAM Sbjct: 383 NQLPQRLNAHGSAMSQTLRNVAGALGTAFLVTLMTNKAASETKHMVATQGIDPNDKAAMA 442 Query: 421 KLTQDATINGITHAFXXXXXXXXXXXXXXXFIKKTSPQPD 460 + Q+ATI GI H+F FI+K P + Sbjct: 443 HVIQEATITGINHSFVVATWFAVAALVLAFFIRKVKPHEE 482 >B3KDK4.1 B3KDK4_9BACI Drug resistance transporter, EmrB/QacA subfamily Length = 501 Score = 589 bits (1519), Expect = e-166, Method: Compositional matrix adjust. Identities = 283/460 (61%), Positives = 356/460 (77%) Query: 1 VALLNQTLMNVALPKMMEDLKIAANTAQWLTTGFMLVNGVLIPISAYLVEKFTTRQLFTT 60 VA+LNQTLMNVALP +ME+L I AQWLTTGFMLVNGVLIP++AYL+ +F+TRQLF + Sbjct: 28 VAILNQTLMNVALPSIMENLSIKPTVAQWLTTGFMLVNGVLIPVTAYLIARFSTRQLFIS 87 Query: 61 AMMLFSIGTLVCAIGTGFEMIMVGRVIQAVGAGILMPLMNIVFLRIFPIEERGKAMGLMA 120 AM LF+IGTL+CAI F ++ GR++QA GAGILMPLM +VFL IFPIE RGKAMG+M Sbjct: 88 AMTLFTIGTLLCAISPNFAFLLTGRLVQAAGAGILMPLMTVVFLNIFPIENRGKAMGMMG 147 Query: 121 VAMIFAPAVGPTLSGWVVQNYSWRVLFYIVLPLAVFSTLLGMKTMQNVGKLTSPRLDKMG 180 +AMIFAPA+GPTLSG++V++Y WRVLFY++LP +V +TL+G+ ++NV +++ P+LD + Sbjct: 148 IAMIFAPAIGPTLSGYIVEHYDWRVLFYMILPFSVIATLIGVFFLKNVTEVSRPKLDMIA 207 Query: 181 VVLSTLAFGGLLYGFSDAGTDGWNSATVYACLILGVVALALFVWRELKTEKPLLEFRIFR 240 ++LSTL FGGLLYGFSDAG DGW+S V L +G VAL LFVWRE+K + P+LEFRIF+ Sbjct: 208 IILSTLGFGGLLYGFSDAGNDGWSSTPVVTTLAVGTVALILFVWREMKADNPMLEFRIFK 267 Query: 241 YNMYSLTTVINIIVTMALYSGMILLPIYLQTIRGFTPMESGLMLLPGAILMGVMSPITGI 300 YNM++LTT+IN+IVTMA+Y+GMILLPIYLQ IRGFTP+ESGL++LPGAILMG+MSPITG Sbjct: 268 YNMFTLTTLINVIVTMAMYAGMILLPIYLQQIRGFTPVESGLLMLPGAILMGIMSPITGA 327 Query: 301 IFDKIGARWLSVIGLIITTITTWEFSQXXXXXXXXXXXXXXXARMFGMSMLMMPIVTAGL 360 IFDK+GARWL+VIGL IT ITTWEFS ARMFGMSMLMMPI TAGL Sbjct: 328 IFDKVGARWLAVIGLAITVITTWEFSNLTETMSYGQIMIIYTARMFGMSMLMMPIQTAGL 387 Query: 361 NQLPQRLASHGTAMSNTLRTVGGALGMALFVSLMTNRTKSDITEAVMSGSVSQTDKAAML 420 NQLP+ L +HGTAMSNTLR V G++G A+ V++M+ + +S + SG ++A +L Sbjct: 388 NQLPRSLNAHGTAMSNTLRMVSGSIGTAILVTVMSTQAESRGEALMQSGLFDPKNQADLL 447 Query: 421 KLTQDATINGITHAFXXXXXXXXXXXXXXXFIKKTSPQPD 460 KL +ATI GI +AF FIKKT P + Sbjct: 448 KLGNEATIYGINYAFVIATWISVVSLILAFFIKKTKPAEE 487 >B1DHG1.1 B1DHG1_9BACL Drug resistance transporter, EmrB/QacA subfamily Length = 592 Score = 581 bits (1498), Expect = e-164, Method: Compositional matrix adjust. Identities = 284/473 (60%), Positives = 357/473 (75%), Gaps = 2/473 (0%) Query: 1 VALLNQTLMNVALPKMMEDLKIAANTAQWLTTGFMLVNGVLIPISAYLVEKFTTRQLFTT 60 VALL+QT++NVALPKMM DL + +T QWL+ G+MLVNGVL+PISAYL+ +FTTR+LF Sbjct: 24 VALLSQTVLNVALPKMMADLDVGESTIQWLSNGYMLVNGVLVPISAYLINRFTTRKLFLV 83 Query: 61 AMMLFSIGTLVCAIGTGFEMIMVGRVIQAVGAGILMPLMNIVFLRIFPIEERGKAMGLMA 120 A LF+IGT+VCA+ F ++ GR++QA GAGILMPLM IV L IFP+EERGKAMGLM Sbjct: 84 ASSLFAIGTVVCALSGDFSSLLAGRLVQACGAGILMPLMTIVTLTIFPVEERGKAMGLMG 143 Query: 121 VAMIFAPAVGPTLSGWVVQNYSWRVLFYIVLPLAVFSTLLGMKTMQNVGKLTSPRLDKMG 180 VAMIFAPAVGPTLSGWVV++Y W VLFYIVLPLA+ + + G +M++V K + P LD + Sbjct: 144 VAMIFAPAVGPTLSGWVVEHYDWHVLFYIVLPLAILAVVFGAFSMKDVIKTSRPNLDILS 203 Query: 181 VVLSTLAFGGLLYGFSDAGTDGWNSATVYACLILGVVALALFVWRELKTEKPLLEFRIFR 240 VVLSTL FGGLLYGFS+AGT+GW+S V CL +G ++L LFV R + + PLLE R+F+ Sbjct: 204 VVLSTLGFGGLLYGFSEAGTEGWDSTEVIVCLAVGAISLILFVVRSILMKAPLLEMRVFK 263 Query: 241 YNMYSLTTVINIIVTMALYSGMILLPIYLQTIRGFTPMESGLMLLPGAILMGVMSPITGI 300 Y+M+SLT +IN I+TMA++SGMILLPI+LQ IRGFTP+ESGL+LLPGAILMG+MSPITG+ Sbjct: 264 YSMFSLTALINAIITMAMFSGMILLPIFLQNIRGFTPLESGLLLLPGAILMGIMSPITGM 323 Query: 301 IFDKIGARWLSVIGLIITTITTWEFSQXXXXXXXXXXXXXXXARMFGMSMLMMPIVTAGL 360 +FDKIGARWL+VIGLIITT+TT+EFS RMFGMSMLMMPI TAGL Sbjct: 324 VFDKIGARWLAVIGLIITTVTTYEFSHLEIDSSYNHMMLFYTLRMFGMSMLMMPIQTAGL 383 Query: 361 NQLPQRLASHGTAMSNTLRTVGGALGMALFVSLMTNRTKSDITEAVMSGSVSQTDKAAML 420 NQLP+RL +HG+AMS TLR V GALG A+ V++MTN+ S E +++G++S +DKA M Sbjct: 384 NQLPRRLNAHGSAMSQTLRNVSGALGTAILVTIMTNKAASHAKELIIAGNISPSDKAKMG 443 Query: 421 KLTQDATINGITHAFXXXXXXXXXXXXXXXFIKKTSPQPDFLQTED--TEPVQ 471 ++T +TI GI +F FI+K P D +Q D E VQ Sbjct: 444 EITIQSTIYGINQSFVVATWLTVAALVLAFFIRKVKPAEDAVQVPDKAAEAVQ 496 >C1P747.1 C1P747_BACCO Drug resistance transporter, EmrB/QacA subfamily Length = 500 Score = 494 bits (1272), Expect = e-138, Method: Compositional matrix adjust. Identities = 238/472 (50%), Positives = 329/472 (69%), Gaps = 1/472 (0%) Query: 1 VALLNQTLMNVALPKMMEDLKIAANTAQWLTTGFMLVNGVLIPISAYLVEKFTTRQLFTT 60 VA LNQTL+NVALP++M L I+A+TA WL+T +ML NG++IP++AYL+ +FTTRQLF Sbjct: 26 VAFLNQTLINVALPQIMNHLHISASTANWLSTIYMLTNGIVIPVTAYLMSRFTTRQLFLF 85 Query: 61 AMMLFSIGTLVCAIGTGFEMIMVGRVIQAVGAGILMPLMNIVFLRIFPIEERGKAMGLMA 120 ++ LF++GTL+CA+ GF ++++GRV+QA GAGI+ PL+ V +FP + RG MG+ Sbjct: 86 SIGLFAVGTLICAVAPGFSVLLIGRVVQAAGAGIIFPLVTNVIFVLFPPDRRGFVMGIFG 145 Query: 121 VAMIFAPAVGPTLSGWVVQNYSWRVLFYIVLPLAVFSTLLGMKTMQNVGKLTSPRLDKMG 180 VAM FAPA+GPTLSGW+V+N+SWR LF+I+LP+A+ + ++NV + + P+LDK+G Sbjct: 146 VAMNFAPAIGPTLSGWIVENHSWRTLFFIILPIAIIDLIFAFVFVKNVTETSRPKLDKIG 205 Query: 181 VVLSTLAFGGLLYGFSDAGTDGWNSATVYACLILGVVALALFVWRELKTEKPLLEFRIFR 240 V+LS+L FGG+LYGFS GT GW+S V L +GV+A LFVWR+ + P+LEFRIFR Sbjct: 206 VILSSLGFGGILYGFSTGGTKGWDSVEVMTTLAVGVIAFGLFVWRQWVIDHPILEFRIFR 265 Query: 241 YNMYSLTTVINIIVTMALYSGMILLPIYLQTIRGFTPMESGLMLLPGAILMGVMSPITGI 300 Y +++LT VINIIVTMAL+SGMIL+PIY+Q +RGF+P+ SGLMLLPG ++MG+MSPITG Sbjct: 266 YPVFTLTMVINIIVTMALFSGMILMPIYMQNVRGFSPLMSGLMLLPGGVIMGIMSPITGR 325 Query: 301 IFDKIGARWLSVIGLIITTITTWEFSQXXXXXXXXXXXXXXXARMFGMSMLMMPIVTAGL 360 +FD+ GA+WL++IGL IT +TT ++ R FG+S+LMMPI TAGL Sbjct: 326 LFDRYGAKWLALIGLAITIVTTVALTRLKLDTSFTYVVTIYTIRSFGISILMMPIFTAGL 385 Query: 361 NQLPQRLASHGTAMSNTLRTVGGALGMALFVSLMTNRTKSDITEAVMSGSVSQTDKAAML 420 N LP + +GTAM NT R V GA+G A FVS+MTN+ + + ++S +S DK Sbjct: 386 NDLPLAMNKYGTAMVNTFRMVAGAVGTAFFVSVMTNKGIAHTKDIMVSSQISPADKIHAA 445 Query: 421 KLTQDATINGITHAFXXXXXXXXXXXXXXXFIKKTSPQPDFLQTEDTEPVQP 472 + T+ GI AF FI+KT P+ D L + V+P Sbjct: 446 LAARQGTVMGINDAFMVATYLAVIAFILAFFIRKTIPR-DSLPENTAQQVKP 496 >C1PAM8.1 C1PAM8_BACCO Drug resistance transporter, EmrB/QacA subfamily Length = 608 Score = 484 bits (1247), Expect = e-135, Method: Compositional matrix adjust. Identities = 240/488 (49%), Positives = 333/488 (68%), Gaps = 28/488 (5%) Query: 1 VALLNQTLMNVALPKMMEDLKIAANTAQWLTTGFMLVNGVLIPISAYLVEKFTTRQLFTT 60 VA+LNQTL+NVA+P +M DL + ANT QWL+TG+MLVNG+L+P++A+++ K+ TR++ T Sbjct: 118 VAILNQTLLNVAIPHIMNDLNVTANTVQWLSTGYMLVNGILVPVTAFMISKWGTRKMLIT 177 Query: 61 AMMLFSIGTLVCAIGTGFEMIMVGRVIQAVGAGILMPLMNIVFLRIFPIEERGKAMGLMA 120 A+ LF+ G+++CAI T F ++M+GR++QA GAGI+MPLM VFL IFP E+RG AMG+M Sbjct: 178 AVSLFTAGSVLCAISTNFSILMLGRIVQASGAGIIMPLMMTVFLTIFPPEKRGSAMGMMG 237 Query: 121 VAMIFAPAVGPTLSGWVVQNYSWRVLFYIVLPLAVFSTLLGMKTMQNVGKLTSPRLDKMG 180 VAMIFAPA+GPTLSGW+V +Y W +LF+IV+P V + + M++V K T+P++D G Sbjct: 238 VAMIFAPAIGPTLSGWLVGHYDWHILFWIVIPFGVIDIFVTLAWMKDVMKTTNPKIDIPG 297 Query: 181 VVLSTLAFGGLLYGFSDAGTDGWNSATVYACLILGVVALALFVWRELKTEKPLLEFRIFR 240 ++ STL FG LLYGFS+AG DGW+S V LI+ V++L LFVWREL TEKP+L+ R+F+ Sbjct: 298 IIFSTLGFGFLLYGFSEAGNDGWSSKQVVISLIIAVISLVLFVWRELTTEKPMLDLRVFK 357 Query: 241 YNMYSLTTVINIIVTMALYSGMILLPIYLQTIRGFTPMESGLMLLPGAILMGVMSPITGI 300 Y++++LTT+++++V MA+++GMILLPIYLQ IRGFT ++SGL++LPGAI+MG+MSPI+G Sbjct: 358 YDIFALTTIVSMVVNMAMFAGMILLPIYLQNIRGFTALDSGLLMLPGAIVMGIMSPISGW 417 Query: 301 IFDKIGARWLSVIGLIITTITTWEFSQXXXXXXXXXXXXXXXARMFGMSMLMMPIVTAGL 360 +FDK+GAR L+V GLIIT TT+EF++ R FGMS +MM I+T G+ Sbjct: 418 LFDKLGARPLAVFGLIITVWTTYEFTKLSMTTSYGHLLFLYVLRSFGMSFIMMTIMTEGM 477 Query: 361 NQLPQRLASHGTAMSNTLRTVGGALGMALFVSLMTNRTKSDITEAV-------------- 406 NQLP + SHGTA +NT RTV G+LG A V++M+ R V Sbjct: 478 NQLPIHMTSHGTAAANTARTVAGSLGTAFLVTVMSTRADFHTANYVNAYANINPYVSAKI 537 Query: 407 ---------MSGSVSQTDKA-----AMLKLTQDATINGITHAFXXXXXXXXXXXXXXXFI 452 SG SQ + K +ATI+GI AF FI Sbjct: 538 AAIGQGLAHASGLPSQYGQVLGSSIVYQKSVVNATISGINDAFFVATGIAAFALILSFFI 597 Query: 453 KKTSPQPD 460 K+ PQP+ Sbjct: 598 KRAMPQPN 605 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.138 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,422,028,600 Number of extensions: 93635573 Number of successful extensions: 441461 Number of sequences better than 1.0e-05: 10525 Number of HSP's gapped: 425384 Number of HSP's successfully gapped: 10546 Length of query: 480 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 340 Effective length of database: 1,741,754,103 Effective search space: 592196395020 Effective search space used: 592196395020 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)