BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0443.0024.p (335 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K583.1 B3K583_9BACI UV-endonuclease UvdE 468 e-130 B1D8T1.1 B1D8T1_9BACL UV-endonuclease UvdE 371 e-101 C2Y517.1 C2Y517_BACCE UV DNA damage endonuclease 292 3e-77 C3EF54.1 C3EF54_BACTK UV DNA damage endonuclease 291 8e-77 B7IU28.1 B7IU28_BACC2 Putative UV-endonuclease 290 2e-76 >B3K583.1 B3K583_9BACI UV-endonuclease UvdE Length = 333 Score = 468 bits (1205), Expect = e-130, Method: Compositional matrix adjust. Identities = 228/335 (68%), Positives = 262/335 (78%), Gaps = 2/335 (0%) Query: 1 MIVRFGYVAMSTVIPDCSPSKTMTMASFNKLNDREAGLRRLEAIARGNLHNTLRLLKHNV 60 MIVRFGYVAMSTV+ + SPSKTMTM SF KLNDREAGL +LE IA NLHNTLRLLKHN Sbjct: 1 MIVRFGYVAMSTVVQNASPSKTMTMKSFMKLNDREAGLHKLERIANENLHNTLRLLKHNA 60 Query: 61 GSDIRVYRLTSKLIPLATHPDLQDWNPFEALAADFAEAGSYVKKHGLRVSFHPDHFTVLS 120 DI+VYR +SKLIPLATH D++DW+PF AL F G +VKKHG+RVSFHPDHFTVLS Sbjct: 61 AHDIKVYRFSSKLIPLATHNDMRDWDPFPALQEGFQAIGEFVKKHGMRVSFHPDHFTVLS 120 Query: 121 TPRPEVLASSVRDLQHHVDMLNAMDLPATAKNNIHIGGAYGDKPAAAARFLEHFAELPLD 180 TPRPEVL +SVRDL+ HV ML AM+LPA+ KNNIHIGGAYGDKP+AAARF+EHF EL Sbjct: 121 TPRPEVLVNSVRDLESHVRMLQAMELPASMKNNIHIGGAYGDKPSAAARFVEHFHELDPR 180 Query: 181 LQERTTLENDDKTFNAPETLEVSRRLGVPMVLDIHHQWVNNEGELPWELWPEIMKTWQTP 240 ++ER TLENDDKTF A ETL V ++ G+PMVLDIHHQWVNN+GE PWELWP+I++TW+T Sbjct: 181 IKERLTLENDDKTFTAQETLAVCQQTGLPMVLDIHHQWVNNDGEAPWELWPDILRTWETQ 240 Query: 241 LALKDVLPGETLPPKIHVXXXXXXXXXXXHADGVEPAPLLAFLKRIAADTPAVDAMIEAK 300 LA D P + LPPKIHV HADGV+ PLLAFL+ IA TP VD MIEAK Sbjct: 241 LAQADSEPAQPLPPKIHVSSPKSKTDIRSHADGVDVVPLLAFLRNIAGVTPKVDVMIEAK 300 Query: 301 AKDGALFGLMEALKELAEAGNGITVLDGASVNIEP 335 KDGALF LM+ L +E GIT+LDGAS+ + P Sbjct: 301 MKDGALFDLMKDLGNYSE--EGITILDGASIEVNP 333 >B1D8T1.1 B1D8T1_9BACL UV-endonuclease UvdE Length = 338 Score = 371 bits (953), Expect = e-101, Method: Compositional matrix adjust. Identities = 186/343 (54%), Positives = 235/343 (68%), Gaps = 13/343 (3%) Query: 1 MIVRFGYVAMSTVIPDCSPSKTMTMASFNKLNDREAGLRRLEAIARGNLHNTLRLLKHNV 60 MIVRFG+VAMST++ + SPS+TMT A+F+KLNDREAG+R++E IA NLH++LR++KH Sbjct: 1 MIVRFGFVAMSTILENASPSRTMTFANFSKLNDREAGIRKIERIAEENLHSSLRIMKHAN 60 Query: 61 GSDIRVYRLTSKLIPLATHPDLQDWNPFEALAADFAEAGSYVKKHGLRVSFHPDHFTVLS 120 DI +YR +SKLIPLATH DL DW+PF L E G Y KK G+R SFHPDHF V S Sbjct: 61 AHDIHMYRFSSKLIPLATHEDLLDWDPFPILRPSMKEVGDYAKKTGMRTSFHPDHFCVFS 120 Query: 121 TPRPEVLASSVRDLQHHVDMLNAMDLPATAKNNIHIGGAYGDKPAAAARFLEHFAELPLD 180 T RP+VL S RDL +H+ +L AM+LP T K NIH+GGAYGDK + RF++ F LP Sbjct: 121 TNRPDVLVKSERDLDYHIRILEAMELPETVKCNIHMGGAYGDKKLSGERFVQQFGALPDR 180 Query: 181 LQERTTLENDDKTFNAPETLEVSRRLGVPMVLDIHHQWVNNEGELPWE-----LWPEIMK 235 + R TLENDDKTF+ ET+ + +GVPMVLDIHH VN+ G++P + LWP I + Sbjct: 181 YKHRVTLENDDKTFDVRETIAAAEAVGVPMVLDIHHHAVNS-GDVPMDELSGGLWPRIAR 239 Query: 236 TW---QTPLALKDVLPGETLPPKIHVXXXXXXXXXXXHADGVEPAPLLAFLKRIAADTPA 292 TW + L L++ LPPKIH HAD VEP PL+ FL+ +AA TP Sbjct: 240 TWTLEEQRLGLEE----GGLPPKIHASSPKSLSDPRGHADNVEPEPLVQFLRSVAAATPR 295 Query: 293 VDAMIEAKAKDGALFGLMEALKELAEAGNGITVLDGASVNIEP 335 +D M+EAK KD AL LME LKE+A GNGI +LDG +V I+P Sbjct: 296 IDCMLEAKHKDAALLKLMEDLKEMAALGNGIRILDGGTVEIDP 338 >C2Y517.1 C2Y517_BACCE UV DNA damage endonuclease Length = 320 Score = 292 bits (748), Expect = 3e-77, Method: Compositional matrix adjust. Identities = 146/334 (43%), Positives = 198/334 (59%), Gaps = 14/334 (4%) Query: 1 MIVRFGYVAMSTVIPDCSPSKTMTMASFNKLNDREAGLRRLEAIARGNLHNTLRLLKHNV 60 M+VR GYVAMS + + SPS+TMT A F KL+DR+A +R+LE IA NL N LRLLKHN Sbjct: 1 MLVRLGYVAMSVHLKNASPSQTMTYAQFQKLDDRDAAIRKLERIANSNLENCLRLLKHNK 60 Query: 61 GSDIRVYRLTSKLIPLATHPDLQDWNPFEALAADFAEAGSYVKKHGLRVSFHPDHFTVLS 120 G DI +RL+SKLIPLA H +L +WN L +F + G Y +R+ FHPDHF VL+ Sbjct: 61 GHDISFFRLSSKLIPLANHEELLEWNYIRPLKENFKKLGEYAVHMNMRIDFHPDHFVVLN 120 Query: 121 TPRPEVLASSVRDLQHHVDMLNAMDLPATAKNNIHIGGAYGDKPAAAARFLEHFAELPLD 180 +P V SV+ LQ H +L M + + +H+GG Y DK A RF+E+++ +P Sbjct: 121 SPEESVFKQSVKTLQMHKKLLKGMGIEHKQRCVLHVGGGYKDKELALERFIENWSNVPRG 180 Query: 181 LQERTTLENDDKTFNAPETLEVSRRLGVPMVLDIHHQWVNNEGELPWELWPEIMKTWQTP 240 +QE LENDD TF ETL + +L +P+V D+HH +NNE E +E W ++ TW+T Sbjct: 181 IQEMIILENDDTTFTLEETLYLGEKLDIPVVFDLHHHMMNNEQEDWYEDWARVVHTWETS 240 Query: 241 LALKDVLPGETLPPKIHVXXXXXXXXXXXHADGVEPAPLLAFLKRIAADTPAVDAMIEAK 300 L LP K+H+ HAD + L+FL+RI P +D MIEAK Sbjct: 241 L----------LPIKMHISSPREGKDPRAHADYINVDAFLSFLRRIKGSVPQIDCMIEAK 290 Query: 301 AKDGALFGLMEALKELAEAGNGITVLDGASVNIE 334 KD +LF LM L E + + ++DGAS I+ Sbjct: 291 MKDESLFQLMRDLSEQVD----VEIIDGASFYIK 320 >C3EF54.1 C3EF54_BACTK UV DNA damage endonuclease Length = 320 Score = 291 bits (744), Expect = 8e-77, Method: Compositional matrix adjust. Identities = 146/334 (43%), Positives = 195/334 (58%), Gaps = 14/334 (4%) Query: 1 MIVRFGYVAMSTVIPDCSPSKTMTMASFNKLNDREAGLRRLEAIARGNLHNTLRLLKHNV 60 M+VR GYVAMS + + SPS+TMT A F KLNDR A +R+LE IA NL N LRLLKHN Sbjct: 1 MLVRLGYVAMSVHLKNASPSQTMTYAQFQKLNDRAAAIRKLERIANSNLENCLRLLKHNK 60 Query: 61 GSDIRVYRLTSKLIPLATHPDLQDWNPFEALAADFAEAGSYVKKHGLRVSFHPDHFTVLS 120 G DI +RL+SKLIPLA H +L +WN L + + G Y +R+ FHPDHF VL+ Sbjct: 61 GHDISFFRLSSKLIPLANHEELLEWNYIRPLKENLKKLGEYAVHMNMRIDFHPDHFVVLN 120 Query: 121 TPRPEVLASSVRDLQHHVDMLNAMDLPATAKNNIHIGGAYGDKPAAAARFLEHFAELPLD 180 +P V SV+ LQ H +L M + + +H+GG Y DK A RF+E ++ +P Sbjct: 121 SPEESVFKQSVKTLQMHKKLLKGMGIEHKQRCVLHVGGGYKDKELALERFIEKWSNVPRG 180 Query: 181 LQERTTLENDDKTFNAPETLEVSRRLGVPMVLDIHHQWVNNEGELPWELWPEIMKTWQTP 240 +QE LENDD TF ETL + +L +P+V D+HH +NNE E +E W ++ TW+T Sbjct: 181 IQEMIILENDDTTFTLEETLYLGEKLDIPVVFDLHHHMMNNEQEDWYEDWARVVHTWETS 240 Query: 241 LALKDVLPGETLPPKIHVXXXXXXXXXXXHADGVEPAPLLAFLKRIAADTPAVDAMIEAK 300 L LP K+H+ HAD + L+FL+RI P +D MIEAK Sbjct: 241 L----------LPVKMHISSPREGKDPRAHADYINVEAFLSFLRRIKGSVPQIDCMIEAK 290 Query: 301 AKDGALFGLMEALKELAEAGNGITVLDGASVNIE 334 KD +LF LM L E + + ++DGAS I+ Sbjct: 291 MKDESLFQLMRDLSEQVD----VEIIDGASFYIK 320 >B7IU28.1 B7IU28_BACC2 Putative UV-endonuclease Length = 320 Score = 290 bits (741), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 145/334 (43%), Positives = 196/334 (58%), Gaps = 14/334 (4%) Query: 1 MIVRFGYVAMSTVIPDCSPSKTMTMASFNKLNDREAGLRRLEAIARGNLHNTLRLLKHNV 60 M+VR GYVAMS + + SPS+TMT A F K++DR+A +R+LE IA NL N LRLLKHN Sbjct: 1 MLVRLGYVAMSVHLKNASPSQTMTYAQFQKIDDRDAAIRKLERIANSNLENCLRLLKHNK 60 Query: 61 GSDIRVYRLTSKLIPLATHPDLQDWNPFEALAADFAEAGSYVKKHGLRVSFHPDHFTVLS 120 G DI +RL+SKLIPLA H +L +WN L E G Y +R+ FHPDHF VL+ Sbjct: 61 GHDISFFRLSSKLIPLANHEELLEWNYIRPLKEKLKELGEYAIHMNMRIDFHPDHFVVLN 120 Query: 121 TPRPEVLASSVRDLQHHVDMLNAMDLPATAKNNIHIGGAYGDKPAAAARFLEHFAELPLD 180 +P V SV+ LQ H +L M + + +H+GG Y DK A RF+E+++ +P Sbjct: 121 SPEESVFKQSVKTLQMHKKLLKGMGIEHKQRCVLHVGGGYKDKELALERFIENWSNVPRG 180 Query: 181 LQERTTLENDDKTFNAPETLEVSRRLGVPMVLDIHHQWVNNEGELPWELWPEIMKTWQTP 240 +QE LENDD TF ETL + +L +P+V D+HH +NNE E +E W ++ TW+T Sbjct: 181 IQEMIILENDDTTFTLEETLYLGEKLDIPVVFDLHHHMMNNEQEDWYEDWARVVHTWETS 240 Query: 241 LALKDVLPGETLPPKIHVXXXXXXXXXXXHADGVEPAPLLAFLKRIAADTPAVDAMIEAK 300 L LP K+H+ HAD + L+FL+RI P +D MIEAK Sbjct: 241 L----------LPVKMHISSPREGKDPRAHADYINVDAFLSFLRRIKGSVPQIDCMIEAK 290 Query: 301 AKDGALFGLMEALKELAEAGNGITVLDGASVNIE 334 KD +LF LM L E + + ++DGAS I+ Sbjct: 291 MKDESLFQLMRDLSEQVD----VEIIDGASFYIK 320 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.135 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,008,501,231 Number of extensions: 83119050 Number of successful extensions: 237795 Number of sequences better than 1.0e-05: 285 Number of HSP's gapped: 237266 Number of HSP's successfully gapped: 285 Length of query: 335 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 199 Effective length of database: 1,779,438,163 Effective search space: 354108194437 Effective search space used: 354108194437 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)