BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0443.0022.p (363 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K581.1 B3K581_9BACI D-alanine--D-alanine ligase 566 e-159 B1D8S9.1 B1D8S9_9BACL D-alanine--D-alanine ligase EC=6.3.2.4 429 e-118 A7GKE0.1 DDL_BACCN D-alanine--D-alanine ligase EC=6.3.2.4 405 e-111 C2ZIW2.1 C2ZIW2_BACCE D-alanine--D-alanine ligase 400 e-109 C2Z282.1 C2Z282_BACCE D-alanine--D-alanine ligase 400 e-109 >B3K581.1 B3K581_9BACI D-alanine--D-alanine ligase Length = 364 Score = 566 bits (1458), Expect = e-159, Method: Compositional matrix adjust. Identities = 271/364 (74%), Positives = 312/364 (85%), Gaps = 2/364 (0%) Query: 1 MGDTKITVGLVYGGKSGEHEVSLQTAFAVMNAFDYAKYEIIPFYISKQGLWKVGAAMDAP 60 MG K+TVGLVYGGKSGEHEVSL TAFAVMNAFD+ KYEI+PFYISKQG W++G+ M AP Sbjct: 1 MGQDKLTVGLVYGGKSGEHEVSLSTAFAVMNAFDFDKYEIVPFYISKQGTWRIGSKMSAP 60 Query: 61 FGTIQELKLGGEAGDMSMALNAVFSGLTGG-DQLIDVMFPLLHGTNGEDGTIQGLFEMAN 119 F +ELKL G + ALNAVFS L G +Q +D+MFPLLHGT GEDGTIQGLFEMA+ Sbjct: 61 FARQEELKLAEGEGTQT-ALNAVFSRLYGEENQAVDIMFPLLHGTFGEDGTIQGLFEMAD 119 Query: 120 IPYIGAGVLASSAGMDKVVMKKLFGEAGLDQCEYCYFNISAWKRKSHELIISLEDKLGYP 179 IPY+G GVLAS+AGMDKVVMKKLF +AGL QCEYCYFN + W+R HE++ ++EDKLG+P Sbjct: 120 IPYVGTGVLASAAGMDKVVMKKLFAQAGLPQCEYCYFNRTQWERSQHEVLKNIEDKLGFP 179 Query: 180 VFVKPANLGSSVGISKAVDKESLIKAVEVAFRYDTKVIIEEFVDAREVEVSVLGNEEPEA 239 FVKPANLGSSVGISKA ++E L A+E A R+D K+I+EEFVDAREVEVSVLGN+EP A Sbjct: 180 SFVKPANLGSSVGISKAKNREQLKAAIESALRFDDKIIVEEFVDAREVEVSVLGNDEPMA 239 Query: 240 SVPGEIVSSGEYYDYAAKYTDGKSQMLIPAPVDPEVADRLRESAIQAFRAIEGSGITRAD 299 SVPGEIVSS EYYDYAAKYTDG+SQM IPA +DP+ ADRLR+ AI AF+AIEG GITRAD Sbjct: 240 SVPGEIVSSSEYYDYAAKYTDGQSQMQIPAEIDPDTADRLRDLAIAAFKAIEGGGITRAD 299 Query: 300 FFLRKSDGKILINEVNTMPGFTPYSMYPLLWRETGVSYQALLDRMIELALERYHFKQNLK 359 FF+R+SDG ILINEVNTMPGFTP+SMYPLLWRETGVSY+ LLDRMIEL LERYH KQ+L Sbjct: 300 FFVRRSDGAILINEVNTMPGFTPFSMYPLLWRETGVSYRELLDRMIELGLERYHRKQSLI 359 Query: 360 YDNE 363 Y+N+ Sbjct: 360 YENQ 363 >B1D8S9.1 B1D8S9_9BACL D-alanine--D-alanine ligase EC=6.3.2.4 Length = 375 Score = 429 bits (1103), Expect = e-118, Method: Compositional matrix adjust. Identities = 211/366 (57%), Positives = 277/366 (75%), Gaps = 10/366 (2%) Query: 5 KITVGLVYGGKSGEHEVSLQTAFAVMNAFDYAKYEIIPFYISKQGLWKVGAAMDAPFGTI 64 K+ +GL+YGGKSGEH+VSL+TAFAVM DYAKYEI PFYI++ G W+VG+ + G I Sbjct: 4 KVHLGLIYGGKSGEHDVSLRTAFAVMGELDYAKYEITPFYITRLGEWRVGSMLGGQPGAI 63 Query: 65 QELKLGGEAG-------DMSMAL---NAVFSGLTGGDQLIDVMFPLLHGTNGEDGTIQGL 114 EL+L G D+ +A L GG Q IDV+FPLLHGT GEDGTIQGL Sbjct: 64 DELRLAPVDGAPVPIVPDLPLAPLLDRLAGGALPGGGQPIDVVFPLLHGTFGEDGTIQGL 123 Query: 115 FEMANIPYIGAGVLASSAGMDKVVMKKLFGEAGLDQCEYCYFNISAWKRKSHELIISLED 174 E+A+IPY+GAGVLAS+AGMDK+ MKK+F GL QC + YFN + W++ + I+ E Sbjct: 124 LEIADIPYVGAGVLASAAGMDKITMKKIFAYEGLPQCVFRYFNRAQWEKDASFFIMECEV 183 Query: 175 KLGYPVFVKPANLGSSVGISKAVDKESLIKAVEVAFRYDTKVIIEEFVDAREVEVSVLGN 234 LGYP FVKP+NLGSSVGISKA +++ LI A++ AFR+D +VI+EEFVDARE+EVSVLGN Sbjct: 184 ALGYPCFVKPSNLGSSVGISKAKNRDELIAAIDFAFRFDRRVIVEEFVDAREIEVSVLGN 243 Query: 235 EEPEASVPGEIVSSGEYYDYAAKYTDGKSQMLIPAPVDPEVADRLRESAIQAFRAIEGSG 294 ++P+ SVPGEI S E+YDY AKY DGKS + IPA + + AD +R+ A++AF AI+G+G Sbjct: 244 DDPKISVPGEIAPSNEFYDYKAKYVDGKSTIHIPANIPNKTADAVRDMALRAFLAIDGAG 303 Query: 295 ITRADFFLRKSDGKILINEVNTMPGFTPYSMYPLLWRETGVSYQALLDRMIELALERYHF 354 ++R DFFLRK+DG++ INEVNTMPGFTP SMYPL+W+E+G+ Y+ LLD++I+LA +RY Sbjct: 304 LSRVDFFLRKTDGQLFINEVNTMPGFTPISMYPLMWKESGLPYKDLLDKLIDLAFKRYAD 363 Query: 355 KQNLKY 360 ++ + Y Sbjct: 364 RRTIDY 369 >A7GKE0.1 DDL_BACCN D-alanine--D-alanine ligase EC=6.3.2.4 Length = 362 Score = 405 bits (1040), Expect = e-111, Method: Compositional matrix adjust. Identities = 194/360 (53%), Positives = 264/360 (73%), Gaps = 4/360 (1%) Query: 4 TKITVGLVYGGKSGEHEVSLQTAFAVMNAFDYAKYEIIPFYISKQGLWKVGAAMDAPFGT 63 TKI +GL+YGGKS EH+VSLQTA A + A + K+EI P YI++QG W G ++ + Sbjct: 2 TKIKLGLLYGGKSAEHQVSLQTALAAIKALNQEKFEIHPIYITEQGQWMRGERIEGEVTS 61 Query: 64 IQELKLGGEAG---DMSMALNAVFSGLTGGDQLIDVMFPLLHGTNGEDGTIQGLFEMANI 120 +Q L++ GEA +S++ + + + ++ IDV+FPLLHG NGEDGT+QGL E+ NI Sbjct: 62 VQALQMSGEANAISPVSLSTEIIPAPASKQEEAIDVIFPLLHGPNGEDGTVQGLLELMNI 121 Query: 121 PYIGAGVLASSAGMDKVVMKKLFGEAGLDQCEYCYFNISAWKRKSHELIISLEDKLGYPV 180 PY+G GVLAS+AGMDKVVMK +F EAGL+Q +Y F S W++ E +E+ LGYP Sbjct: 122 PYVGNGVLASAAGMDKVVMKNIFAEAGLNQAKYTSFVRSVWEKNREEAYEKVEEALGYPC 181 Query: 181 FVKPANLGSSVGISKAVDKESLIKAVEVAFRYDTKVIIEEFVDAREVEVSVLGNEEPEAS 240 FVKPANLGSSVGI+K D+E L KA E AF++D K+I+EE + REVEV VLGN++P+ S Sbjct: 182 FVKPANLGSSVGINKCKDREELEKAFEEAFQFDRKIIVEENIIGREVEVGVLGNDDPKCS 241 Query: 241 VPGEIVSSGEYYDYAAKYTDGKSQMLIPAPVDPEVADRLRESAIQAFRAIEGSGITRADF 300 V GEIV E+YDY +KY DG + ++IPA + E ++ AI+AF+A++G+G+TRADF Sbjct: 242 VVGEIVPKKEFYDYKSKYIDGDTALIIPAEITEEETATIQRDAIRAFQALDGAGLTRADF 301 Query: 301 FLRKSDGKILINEVNTMPGFTPYSMYPLLWRETGVSYQALLDRMIELALERYHFKQNLKY 360 FL K DG++ INEVNTMPGFTP+SM+PLLW+ TG+SY L++ +I LA+ER+ KQ +KY Sbjct: 302 FLTK-DGEVYINEVNTMPGFTPFSMFPLLWQHTGLSYPELIEELIRLAIERHKEKQKIKY 360 >C2ZIW2.1 C2ZIW2_BACCE D-alanine--D-alanine ligase Length = 361 Score = 400 bits (1027), Expect = e-109, Method: Compositional matrix adjust. Identities = 195/359 (54%), Positives = 261/359 (72%), Gaps = 3/359 (0%) Query: 4 TKITVGLVYGGKSGEHEVSLQTAFAVMNAFDYAKYEIIPFYISKQGLWKVGAAMDAPFGT 63 TKI +GL+YGGKS EH+VSLQTA A + A + K+EI P YI++QG W G ++ Sbjct: 2 TKIKLGLLYGGKSAEHQVSLQTALAAIKALNQDKFEIHPIYITEQGQWVRGERIEGEVTD 61 Query: 64 IQELKLGGEAGDMS-MALNA-VFSGLTGGDQLIDVMFPLLHGTNGEDGTIQGLFEMANIP 121 ++ LK+ G +S ++L+ + T + IDV+FPLLHG NGEDGT+QGL E+ NIP Sbjct: 62 VEALKMSGAENAISPLSLSTEIIPAETSEENAIDVIFPLLHGPNGEDGTVQGLLELMNIP 121 Query: 122 YIGAGVLASSAGMDKVVMKKLFGEAGLDQCEYCYFNISAWKRKSHELIISLEDKLGYPVF 181 Y+G GVLASSAGMDKVVMK +F EAGL Q +Y F SAW++ E +E+KLGYP F Sbjct: 122 YVGNGVLASSAGMDKVVMKNIFAEAGLKQAKYASFIRSAWEKNREEAYDKVEEKLGYPCF 181 Query: 182 VKPANLGSSVGISKAVDKESLIKAVEVAFRYDTKVIIEEFVDAREVEVSVLGNEEPEASV 241 VKPANLGSSVGI+K D+E L A E AF++D K+I+EE + REVEV VLGN+EP+ SV Sbjct: 182 VKPANLGSSVGINKCKDREELENAFEEAFQFDRKIIVEENIVGREVEVGVLGNDEPKCSV 241 Query: 242 PGEIVSSGEYYDYAAKYTDGKSQMLIPAPVDPEVADRLRESAIQAFRAIEGSGITRADFF 301 GEIV ++YDY +KY DG + ++IPA + E +D ++ AI AF++++G+G+TRADFF Sbjct: 242 VGEIVPKKDFYDYKSKYIDGDTALIIPAEMTEEESDVIKRDAIVAFQSLDGAGLTRADFF 301 Query: 302 LRKSDGKILINEVNTMPGFTPYSMYPLLWRETGVSYQALLDRMIELALERYHFKQNLKY 360 L K DG++ INEVNTMPGFTP+SM+PLLW+ TG+ Y L++ +I LA+ER+ KQ +KY Sbjct: 302 LTK-DGEVYINEVNTMPGFTPFSMFPLLWQHTGLPYPKLIEELINLAIERHEEKQKIKY 359 >C2Z282.1 C2Z282_BACCE D-alanine--D-alanine ligase Length = 361 Score = 400 bits (1027), Expect = e-109, Method: Compositional matrix adjust. Identities = 195/359 (54%), Positives = 261/359 (72%), Gaps = 3/359 (0%) Query: 4 TKITVGLVYGGKSGEHEVSLQTAFAVMNAFDYAKYEIIPFYISKQGLWKVGAAMDAPFGT 63 TKI +GL+YGGKS EH+VSLQTA A + A + K+EI P YI++QG W G ++ Sbjct: 2 TKIKLGLLYGGKSAEHQVSLQTALAAIKALNQDKFEIHPIYITEQGQWVRGERIEGEVTD 61 Query: 64 IQELKLGGEAGDMS-MALNA-VFSGLTGGDQLIDVMFPLLHGTNGEDGTIQGLFEMANIP 121 ++ LK+ G +S ++L+ + T + IDV+FPLLHG NGEDGT+QGL E+ NIP Sbjct: 62 VEALKMSGAENAISPLSLSTEIIPAETSEENAIDVIFPLLHGPNGEDGTVQGLLELMNIP 121 Query: 122 YIGAGVLASSAGMDKVVMKKLFGEAGLDQCEYCYFNISAWKRKSHELIISLEDKLGYPVF 181 Y+G GVLASSAGMDKVVMK +F EAGL Q +Y F SAW++ E +E+KLGYP F Sbjct: 122 YVGNGVLASSAGMDKVVMKNIFAEAGLKQAKYASFIRSAWEKNREEAYDKVEEKLGYPCF 181 Query: 182 VKPANLGSSVGISKAVDKESLIKAVEVAFRYDTKVIIEEFVDAREVEVSVLGNEEPEASV 241 VKPANLGSSVGI+K D+E L A E AF++D K+I+EE + REVEV VLGN+EP+ SV Sbjct: 182 VKPANLGSSVGINKCKDREELENAFEEAFQFDRKIIVEENIVGREVEVGVLGNDEPKCSV 241 Query: 242 PGEIVSSGEYYDYAAKYTDGKSQMLIPAPVDPEVADRLRESAIQAFRAIEGSGITRADFF 301 GEIV ++YDY +KY DG + ++IPA + E +D ++ AI AF++++G+G+TRADFF Sbjct: 242 VGEIVPKKDFYDYKSKYIDGDTALIIPAEMTEEESDVIKRDAIVAFQSLDGAGLTRADFF 301 Query: 302 LRKSDGKILINEVNTMPGFTPYSMYPLLWRETGVSYQALLDRMIELALERYHFKQNLKY 360 L K DG++ INEVNTMPGFTP+SM+PLLW+ TG+ Y L++ +I LA+ER+ KQ +KY Sbjct: 302 LTK-DGEVYINEVNTMPGFTPFSMFPLLWQHTGLPYPKLIEELINLAIERHEEKQKIKY 359 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.137 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,226,304,680 Number of extensions: 94750466 Number of successful extensions: 245083 Number of sequences better than 1.0e-05: 2298 Number of HSP's gapped: 239596 Number of HSP's successfully gapped: 2317 Length of query: 363 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 226 Effective length of database: 1,770,017,148 Effective search space: 400023875448 Effective search space used: 400023875448 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)