BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0443.0014.p (569 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C4IIT8.1 C4IIT8_CLOBU Integral membrane sensor signal transducti... 386 e-105 B1QSX6.1 B1QSX6_CLOBU Putative sensor histidine kinase 385 e-105 C4Z3B1.1 C4Z3B1_9FIRM Two-component system, sensor histidine kin... 384 e-104 A7VHF0.1 A7VHF0_9CLOT Putative uncharacterized protein 380 e-103 A8SQM7.1 A8SQM7_9FIRM Putative uncharacterized protein 367 2e-99 >C4IIT8.1 C4IIT8_CLOBU Integral membrane sensor signal transduction histidine kinase Length = 567 Score = 386 bits (992), Expect = e-105, Method: Compositional matrix adjust. Identities = 215/569 (37%), Positives = 343/569 (60%), Gaps = 10/569 (1%) Query: 1 MQTWKAFKQKIGDFRFQTKIKLSFLLVSMIPVIVLGSFCFSETRSLLVQQSKADLNATLQ 60 M+ +K FK + +F+ KI ++++V++IP+ ++G ++ TR L+ Q ++ L Sbjct: 1 MKVYKTFK----NMKFRHKIMSTYIIVAIIPITIMGLLLYNHTRKTLLYQENINIKNYLG 56 Query: 61 QSVLAVNNQLDVYNKVMNFLSFNQEIVNAANTTYTSAYEMYDQLNNVIDQNFFTARYLNT 120 Q+V ++NQL +Y+ + ++++FNQ I + Y S Y+MY+Q ++D + +Y + Sbjct: 57 QAVSNMDNQLRIYDNLSDYIAFNQTIGDVVGHKYDSYYDMYNQYTKILDPMLSSLKYFHN 116 Query: 121 GVKQIILYTGTNLPPHGNTVRPIDEIKEASWYPHVMKSVDVLWFSSGR--SVYSARRILS 178 + Q+ +Y + + H T+ P+ +IK WY + ++ ++ W S S +S R + S Sbjct: 117 DINQVTIYANSEIVQHDTTIAPLTDIKNEEWYGLLNENSNIHWLISKEEESAFSVRNMPS 176 Query: 179 TKQENPKDNILFALVDYDALFKTFEPLESHGAEIVITDSNGNSIYS-SGADRTNSGIPIS 237 Q + L+ VDY LF+ F ++ I I D NG I+ S D+ N+ + I+ Sbjct: 177 L-QSDSSLGALYINVDYKKLFEPFRNMKDSNYGIFIIDQNGTDIFEYSVFDKKNNLMEIT 235 Query: 238 -DKEADELHWSGKN-YTVLQSSIPITGWKVYLYKPTSLITKSAWWIALTVLLMXXXXXXX 295 D E + + KN Y V++ W V LYKP SLI + + +++ Sbjct: 236 YDTYLKEKNSTLKNKYAVVECKSETEAWTVVLYKPVSLIYNNITPMLYRSIMIIGLVVII 295 Query: 296 XXXXGTLFSRKIMLPIHRLHKNMKLIGEGNLEIKVFSDSKDEIGDLIRGFGSMLGRTKTL 355 ++ + ++ + +L +NM+ I GN+ I V S S+DE+G L+RGFG M+ + +L Sbjct: 296 SFVISSVLAAHMVSDLEKLRENMEQIESGNMRITVKSTSEDEVGTLVRGFGKMINQINSL 355 Query: 356 IDTVYVGEIARKEYEMKALQAQINPHFLYNSLSLINWRAIRIHATDISEMAQLLSTFYRT 415 I+ VY I +K+YEMKALQAQINPHFLYNSLSLINW AI DIS + LS+FYRT Sbjct: 356 IEDVYKSRIIQKDYEMKALQAQINPHFLYNSLSLINWMAIEADQKDISGITLSLSSFYRT 415 Query: 416 TLNKGENLISVSDEILNVQSYINIQLIMHSNSFEIDYQIDDSILSCRMPNLMLQPLIENA 475 LNKG+N++ V DEILN++SY++IQL+MH F+ + IDD IL ++ NL+LQPLIENA Sbjct: 416 ALNKGKNILLVKDEILNMKSYLDIQLMMHDYEFDAEINIDDEILEYKILNLLLQPLIENA 475 Query: 476 IIHGIENREDGGGIIHLSGKKEDQSIVFLITDNGIGVEQERLALLLKSQSKGYGLKNVND 535 I HGI+ + G G I +SG ++ ++V + DNGIG+EQ+++ +L SKGYG++NVN+ Sbjct: 476 IDHGIDLKRSGRGKITISGYMDNGNVVLSVCDNGIGMEQDKVDTILTENSKGYGVRNVNE 535 Query: 536 RAVVMYGEQYGLTINSIPGEGTQVLLKIP 564 R + YG +Y + INS GEGT ++++IP Sbjct: 536 RIKLYYGSEYEIKINSKIGEGTTMIVRIP 564 >B1QSX6.1 B1QSX6_CLOBU Putative sensor histidine kinase Length = 567 Score = 385 bits (988), Expect = e-105, Method: Compositional matrix adjust. Identities = 215/569 (37%), Positives = 343/569 (60%), Gaps = 10/569 (1%) Query: 1 MQTWKAFKQKIGDFRFQTKIKLSFLLVSMIPVIVLGSFCFSETRSLLVQQSKADLNATLQ 60 M+ +K FK + +F+ KI ++++V++IP+ ++G ++ TR L+ Q ++ L Sbjct: 1 MKVYKTFK----NMKFRHKIMSTYIIVAIIPITIMGLLWYNHTRKTLLYQENINIKNYLG 56 Query: 61 QSVLAVNNQLDVYNKVMNFLSFNQEIVNAANTTYTSAYEMYDQLNNVIDQNFFTARYLNT 120 Q+V ++NQL +Y+ + ++++FNQ I + Y S Y+MY+Q ++D + +Y + Sbjct: 57 QAVSNMDNQLRIYDNLSDYIAFNQTIGDVVGHKYDSYYDMYNQYTKILDPMLSSLKYFHN 116 Query: 121 GVKQIILYTGTNLPPHGNTVRPIDEIKEASWYPHVMKSVDVLWFSSGR--SVYSARRILS 178 + Q+ +Y + + H T+ P+ +IK WY + ++ ++ W S S +S R + S Sbjct: 117 DINQVTIYANSEIVQHDTTIAPLTDIKNEEWYGLLNENSNIHWLISKEEESAFSVRNMPS 176 Query: 179 TKQENPKDNILFALVDYDALFKTFEPLESHGAEIVITDSNGNSIYS-SGADRTNSGIPIS 237 Q + L+ VDY LF+ F ++ I I D NG I+ S D+ N+ + I+ Sbjct: 177 L-QSDSSLGALYINVDYKKLFEPFRNMKDSNYGIFIIDQNGTDIFEYSVFDKKNNLMEIT 235 Query: 238 -DKEADELHWSGKN-YTVLQSSIPITGWKVYLYKPTSLITKSAWWIALTVLLMXXXXXXX 295 D E + + KN Y V++ W V LYKP SLI + + +++ Sbjct: 236 YDTYLKEKNSTLKNKYAVVECKSETEAWTVVLYKPVSLIYNNITPMLYRSIMIIGLVVII 295 Query: 296 XXXXGTLFSRKIMLPIHRLHKNMKLIGEGNLEIKVFSDSKDEIGDLIRGFGSMLGRTKTL 355 ++ + ++ + +L +NM+ I GN+ I V S S+DE+G L+RGFG M+ + +L Sbjct: 296 SFVISSVLAAHMVSDLEKLRENMEQIESGNMRITVKSTSEDEVGTLVRGFGKMINQINSL 355 Query: 356 IDTVYVGEIARKEYEMKALQAQINPHFLYNSLSLINWRAIRIHATDISEMAQLLSTFYRT 415 I+ VY I +K+YEMKALQAQINPHFLYNSLSLINW AI DIS + LS+FYRT Sbjct: 356 IEDVYKSRIIQKDYEMKALQAQINPHFLYNSLSLINWMAIEADQKDISGITLSLSSFYRT 415 Query: 416 TLNKGENLISVSDEILNVQSYINIQLIMHSNSFEIDYQIDDSILSCRMPNLMLQPLIENA 475 LNKG+N++ V DEILN++SY++IQL+MH F+ + IDD IL ++ NL+LQPLIENA Sbjct: 416 ALNKGKNILLVKDEILNMKSYLDIQLMMHDYEFDAEINIDDEILEYKILNLLLQPLIENA 475 Query: 476 IIHGIENREDGGGIIHLSGKKEDQSIVFLITDNGIGVEQERLALLLKSQSKGYGLKNVND 535 I HGI+ + G G I +SG ++ ++V + DNGIG+EQ+++ +L SKGYG++NVN+ Sbjct: 476 IDHGIDLKRSGRGKITISGYMDNGNVVLSVCDNGIGMEQDKVDTILTENSKGYGVRNVNE 535 Query: 536 RAVVMYGEQYGLTINSIPGEGTQVLLKIP 564 R + YG +Y + INS GEGT ++++IP Sbjct: 536 RIKLYYGSEYEIKINSKIGEGTTMIVRIP 564 >C4Z3B1.1 C4Z3B1_9FIRM Two-component system, sensor histidine kinase YesM Length = 585 Score = 384 bits (985), Expect = e-104, Method: Compositional matrix adjust. Identities = 212/584 (36%), Positives = 341/584 (58%), Gaps = 22/584 (3%) Query: 5 KAFKQKIGDFRFQTKIKLSFLLVSMIPVIVLGSFCFSETRSLLVQQSKADLNATLQQSVL 64 K KQKI D R Q K+ + +++ +IP+IVL F + + R++L+ + + T++QSV Sbjct: 2 KKIKQKINDIRLQNKLVIIYVVTGLIPLIVLFVFAYCQMRNILMDRDLKSIKGTIEQSVT 61 Query: 65 AVNNQLDVYNKVMNFLSFNQEIVNAANTTYTSAYEMYDQLNNVIDQNFFTARYLNTGVKQ 124 V+ Q++VY+ + N+++FN + + Y S YEMY+Q+ D + +Y + + + Sbjct: 62 TVDGQIEVYDNLSNYITFNDTLSGVLSYDYKSTYEMYNQIVTTFDPMLSSLKYFHNDINR 121 Query: 125 IILYTGTNLPPHGNTVRPIDEIKEASWYPHVMKSVDVLWF--SSGRSVYSARRILSTKQE 182 + +Y + H T+ PI+EIK+ +Y +S + WF R++ SAR+ +ST + Sbjct: 122 VTIYVDKAIK-HDTTIAPIEEIKDRPFYNSAAESTKIQWFVDEDSRTLVSARK-MSTLDQ 179 Query: 183 NPKDNILFALVDYDALFKTFEPLESHGAEIVITDSNGNSIYSSGA-DRTNSGIPISDKE- 240 I++ VDYD++ +F +V+ D++G I SS + N+ ++ K+ Sbjct: 180 LGILGIMYIDVDYDSMMSSFTGGLEQNCGMVVLDADGKVICSSDTFENNNTRYRLNSKKL 239 Query: 241 ---ADELHWSG------KNYTVLQSSIPITGWKVYLYKPTSLITKSAWWIALTVLLMXXX 291 D W + Y+V++ +TGW VY+Y+P L+ +SA + +T++++ Sbjct: 240 LSLIDGAEWDNDTCGNTEGYSVVKKESSVTGWTVYVYEPRKLVLRSANSM-ITMIVVAFV 298 Query: 292 XXXXXXXXGTLFSRK-IMLPIHRLHKNMKLIGEGNLEIKVFSDSKDEIGDLIRGFGSMLG 350 ++F+ I I++L +M + G+ + + + KDEIGDLI F SM Sbjct: 299 VAVAGAAAASIFTTGFITRRINKLKNSMAKVENGDFDAVINTQDKDEIGDLIHSFNSMTT 358 Query: 351 RTKTLIDTVYVGEIARKEYEMKALQAQINPHFLYNSLSLINWRAIRIHATDISEMAQLLS 410 + K LI VY G I++KE EM+AL+AQINPHFLYNSLSLINW+AI DISE+ LS Sbjct: 359 QIKNLITQVYEGRISQKESEMRALRAQINPHFLYNSLSLINWKAIEYEQEDISEITLALS 418 Query: 411 TFYRTTLNKGENLISVSDEILNVQSYINIQLIMHSNSFEIDYQIDDSILSCRMPNLMLQP 470 +YRT+LNKG+N++S E+ N+QSY+ IQ IMH NSF++ + + ++ C NL+LQP Sbjct: 419 NYYRTSLNKGKNILSFEQELSNMQSYLKIQQIMHDNSFDVVINVSEEVMPCESLNLILQP 478 Query: 471 LIENAIIHGIENREDGGGIIHLSGK----KEDQSIVFL-ITDNGIGVEQERLALLLKSQS 525 L+ENAI HGI+ D G+I + K +E + +V + ++DNG+G+++E L QS Sbjct: 479 LVENAIDHGIDLLTDRKGVITVEAKMQTDEEGKKLVCVTVSDNGVGMDKETAQNFLTVQS 538 Query: 526 KGYGLKNVNDRAVVMYGEQYGLTINSIPGEGTQVLLKIPYDEHN 569 KGYG +NVNDR + YGE Y L + SIP EGT + +K P +N Sbjct: 539 KGYGARNVNDRIRLYYGESYHLEVQSIPDEGTTITIKFPAKPYN 582 >A7VHF0.1 A7VHF0_9CLOT Putative uncharacterized protein Length = 580 Score = 380 bits (976), Expect = e-103, Method: Compositional matrix adjust. Identities = 210/572 (36%), Positives = 332/572 (58%), Gaps = 13/572 (2%) Query: 5 KAFKQKIGDFRFQTKIKLSFLLVSMIPVIVLGSFCFSETRSLLVQQSKADLNATLQQSVL 64 K K++ + R K+ + ++L +P+++ + + E + +L + L + L Q+ Sbjct: 8 KRIKRRFQNLRLSGKLLVIYILAGFLPIMITLTITYHEMKKILWDRETMILESYLTQTTD 67 Query: 65 AVNNQLDVYNKVMNFLSFNQEIVNAANTTYTSAYEMYDQLNNVIDQNFFTARYLNTGVKQ 124 A++N+L++YN + N++S+NQ I ++ S Y+ Y+Q+ +VID + Y + VKQ Sbjct: 68 ALDNELEIYNNLSNYISYNQSIAKILSSD-QSTYQNYEQVASVIDPLLSSIMYFHDDVKQ 126 Query: 125 IILYTGTNLPPHGNTVRPIDEIKEASWYPHVMKSVDVLWF--SSGRSVYSARRILSTKQE 182 + +YT HG+T+ PI ++ ++ + W + ++ YS R+ +Q+ Sbjct: 127 VTIYTDACQVKHGSTIAPITTLENEETNYGIVDDNQIHWIVNAKDKTAYSLNRMAMLEQK 186 Query: 183 NPKDNILFALVDYDALFKTFEP---LESHGAEIVITDSNGNSIYSSGADRTNSGIPISDK 239 K +L+ VDYD++F+ F +++G +VI D+ GN I+ T P Sbjct: 187 KAK-GLLYVGVDYDSVFQPFNNETVFDNYG--VVIEDAEGNCIFKKSV-FTEEKQPYELS 242 Query: 240 EADELHWSGKN---YTVLQSSIPITGWKVYLYKPTSLITKSAWWIALTVLLMXXXXXXXX 296 E G+ Y ++ +GWK+++YKP LI S I + Sbjct: 243 EEQFFATVGQKDSGYQFIKRESKASGWKIWIYKPDRLIISSIQPILIIAAAAILVSLIAA 302 Query: 297 XXXGTLFSRKIMLPIHRLHKNMKLIGEGNLEIKVFSDSKDEIGDLIRGFGSMLGRTKTLI 356 + S I I +L K M+ + GNL +++ +DS DEIGDLI G+ SM R I Sbjct: 303 VACIRIISEFITKRISKLQKKMRNLETGNLGMEIENDSTDEIGDLINGYNSMTKRLDATI 362 Query: 357 DTVYVGEIARKEYEMKALQAQINPHFLYNSLSLINWRAIRIHATDISEMAQLLSTFYRTT 416 + VY +I KEYEM+ALQAQINPHFLYNSLS+INW+A+ DIS + LSTFYRT Sbjct: 363 NEVYRSKIKEKEYEMRALQAQINPHFLYNSLSIINWKALEADQEDISRITLSLSTFYRTA 422 Query: 417 LNKGENLISVSDEILNVQSYINIQLIMHSNSFEIDYQIDDSILSCRMPNLMLQPLIENAI 476 LNKG+N++ V DEI N+++Y++IQL MH +SF++ Y IDDSIL NL+LQP++ENAI Sbjct: 423 LNKGKNILLVRDEIANIKAYLDIQLTMHDDSFDVIYDIDDSILKYETLNLILQPILENAI 482 Query: 477 IHGIENREDGGGIIHLSGKKEDQSIVFLITDNGIGVEQERLALLLKSQSKGYGLKNVNDR 536 HGI+ ++DG G I +SGK++D+ I F ++DNG+G+ QE+ L+L + SKGYG++NVN+R Sbjct: 483 HHGIDLKQDGRGEIRISGKEDDKDIRFAVSDNGVGMTQEQAELILTADSKGYGVRNVNER 542 Query: 537 AVVMYGEQYGLTINSIPGEGTQVLLKIPYDEH 568 + YG +Y + I+S G GT V + P + H Sbjct: 543 IKLYYGAEYAVCIDSEVGRGTTVTIHFPKELH 574 >A8SQM7.1 A8SQM7_9FIRM Putative uncharacterized protein Length = 570 Score = 367 bits (942), Expect = 2e-99, Method: Compositional matrix adjust. Identities = 208/566 (36%), Positives = 322/566 (56%), Gaps = 16/566 (2%) Query: 8 KQKIGDFRFQTKIKLSFLLVSMIPVIVLGSFCFSETRSLLVQQSKADLNATLQQSVLAVN 67 K++ D + K+ + +LL ++PV+++ + E + +L + L + + Q+ +++ Sbjct: 7 KKRFRDMKLTGKMVVIYLLAGLVPVMIILGITYMEMKKILWDRETTILESYVSQTTDSMD 66 Query: 68 NQLDVYNKVMNFLSFNQEIVNAANTTYTSAYEMYDQLNNVIDQNFFTARYLNTGVKQIIL 127 N+L++YN + ++S+NQ I + SAY+ Y+Q + VID + Y + V Q+ + Sbjct: 67 NELEIYNNLSKYISYNQSIAKILSAD-QSAYQNYEQFSTVIDPLLASIMYFHEDVNQVTI 125 Query: 128 YTGTNLPPHGNTVRPIDEIKEASWYPHVMKSVD--VLWFSS--GRSVYSARRILSTKQEN 183 YT + HG+TV P+ ++ + + H +D + W R+ +S R+ +Q+ Sbjct: 126 YTDASDVKHGSTVAPLKDLADGT---HEYDDLDNNIHWHIDLESRTAFSVSRMAMLEQKG 182 Query: 184 PKDNILFALVDYDALFKTFEP---LESHGAEIVITDSNGNSIYSSG--ADRTNSGIPISD 238 K +L+ VDYD++F+ F +++G ++I D G+ I++ AD ++ D Sbjct: 183 AK-GVLYVGVDYDSVFQPFYTETMFDNYG--LIIEDEYGHMIFNKNTFADEQSAYELDVD 239 Query: 239 KEADELHWSGKNYTVLQSSIPITGWKVYLYKPTSLITKSAWWIALTVLLMXXXXXXXXXX 298 K Y + + TGW + +YKP LI S I + + Sbjct: 240 KFDVLREMENSGYQFIDKTSKATGWNICVYKPNRLIISSVRPILIIAVAALLISMLAGIL 299 Query: 299 XGTLFSRKIMLPIHRLHKNMKLIGEGNLEIKVFSDSKDEIGDLIRGFGSMLGRTKTLIDT 358 + S I I L K MK GNL + + +DS DEIGDLI G+ SM R ++ Sbjct: 300 CINMVSEFITKRIKNLQKTMKATETGNLGMVIENDSTDEIGDLINGYNSMSKRLDETVNE 359 Query: 359 VYVGEIARKEYEMKALQAQINPHFLYNSLSLINWRAIRIHATDISEMAQLLSTFYRTTLN 418 VY +I KEYEM+ALQAQINPHFLYNSLS+INW+A+ DIS + LSTFYRT LN Sbjct: 360 VYQSKIKEKEYEMRALQAQINPHFLYNSLSMINWKALEAEQEDISRITLSLSTFYRTALN 419 Query: 419 KGENLISVSDEILNVQSYINIQLIMHSNSFEIDYQIDDSILSCRMPNLMLQPLIENAIIH 478 KG+N++ V DEI N++SY++IQL MH NSF++ Y IDDSIL NL+LQPL+ENAI H Sbjct: 420 KGKNILLVKDEIANIKSYLDIQLAMHDNSFDVVYDIDDSILKYETLNLILQPLLENAIGH 479 Query: 479 GIENREDGGGIIHLSGKKEDQSIVFLITDNGIGVEQERLALLLKSQSKGYGLKNVNDRAV 538 GI+ + DG G I + GK+ + F ++DNG+G+ + + AL+L S GYG+ NVN+R Sbjct: 480 GIDVKTDGRGEIRIEGKENGDFLDFTVSDNGVGMTKTQAALILSKSSNGYGVSNVNERIK 539 Query: 539 VMYGEQYGLTINSIPGEGTQVLLKIP 564 + YGE+Y + I S PG GT+V+L P Sbjct: 540 LYYGEKYAVKIESTPGVGTKVMLHFP 565 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,198,723,773 Number of extensions: 131598525 Number of successful extensions: 376943 Number of sequences better than 1.0e-05: 2018 Number of HSP's gapped: 373407 Number of HSP's successfully gapped: 2018 Length of query: 569 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 428 Effective length of database: 1,732,333,088 Effective search space: 741438561664 Effective search space used: 741438561664 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)