BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0443.0011.p (296 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D919.1 B1D919_9BACL Binding-protein-dependent transport system... 316 1e-84 B1D9Q6.1 B1D9Q6_9BACL Binding-protein-dependent transport system... 310 8e-83 C4ZHX8.1 C4ZHX8_9FIRM Putative uncharacterized protein 307 7e-82 B3KEX2.1 B3KEX2_9BACI Binding-protein-dependent transport system... 304 7e-81 B1DFU4.1 B1DFU4_9BACL Binding-protein-dependent transport system... 303 1e-80 >B1D919.1 B1D919_9BACL Binding-protein-dependent transport systems inner membrane component Length = 296 Score = 316 bits (810), Expect = 1e-84, Method: Compositional matrix adjust. Identities = 154/296 (52%), Positives = 211/296 (71%) Query: 1 MNNKKDQAAFHLMLAPSIVFLIIFSFIPMFGIVMAFQNYIPAKGISDSAWVGLDNFKFMF 60 M Q ++LML P +V+L++FS +PMFGI+MAFQNY P G+ S WVGL+NFK+MF Sbjct: 1 MRKLGHQKHYYLMLLPGMVWLVMFSIVPMFGILMAFQNYNPGSGLFKSEWVGLENFKYMF 60 Query: 61 QIPDSRQILVNTIVIALWKIIVGTIVSIVFALLLNEIRVRFAKKFMQTVVYLPNFLSWAV 120 Q+ DS+ ++ NT++IA+ KI++ IV +VFA+LLNE+R KK +QTVVYLP+FLSW + Sbjct: 61 QLNDSKTVIRNTLIIAVGKIVLNLIVPLVFAILLNELRNLRYKKLVQTVVYLPHFLSWVI 120 Query: 121 LATVVMNIFSYEGPVNAFLGWLGIDPVLFMASNHWFRPMLVLTDVWKGFGYGSIIYLASL 180 ++T+V+ IF Y G VN G+ G DP LFMA FR ++V TDVWK FGY +IIYLA+L Sbjct: 121 MSTIVIGIFGYYGVVNTVFGYFGADPKLFMADAGIFRQLIVGTDVWKEFGYNAIIYLAAL 180 Query: 181 TAIDPGLYEASSIDGANRFKQLWHITLPGLMXXXXXXXXXXXXXXXXAGFDQIFNLYNPL 240 T ++P LYEA++IDGANR++ + H+TLP L+ A FDQ++NLYNPL Sbjct: 181 TGVNPNLYEAAAIDGANRWQLIRHVTLPALITTVVLLGVLSLGNVLNANFDQVYNLYNPL 240 Query: 241 VYATSDIIDTYVYRVGLVERQYSLGTAIGLLRSVVGIVLILSANKLAQKLTDYRIF 296 VY+T D++DT+VYR+GL Q+SL TA GL +SV+ VLI + +LA + DY IF Sbjct: 241 VYSTGDVLDTWVYRLGLQNLQFSLATAAGLFKSVISFVLIYISYRLAYRYADYTIF 296 >B1D9Q6.1 B1D9Q6_9BACL Binding-protein-dependent transport systems inner membrane component Length = 296 Score = 310 bits (795), Expect = 8e-83, Method: Compositional matrix adjust. Identities = 145/296 (48%), Positives = 212/296 (71%) Query: 1 MNNKKDQAAFHLMLAPSIVFLIIFSFIPMFGIVMAFQNYIPAKGISDSAWVGLDNFKFMF 60 M + K +HLMLAP ++ L+IF +P+FG+ +AFQ++IP KGI S WVGL+NFK+MF Sbjct: 1 MRDSKSTLHYHLMLAPGMILLVIFGIVPLFGMAIAFQDFIPVKGIWGSKWVGLENFKYMF 60 Query: 61 QIPDSRQILVNTIVIALWKIIVGTIVSIVFALLLNEIRVRFAKKFMQTVVYLPNFLSWAV 120 ++ DS+ I NTI I++ K+++ ++ + ALLL+EI K++MQT++YLP+FLSW + Sbjct: 61 ELDDSKTIFFNTIFISVMKMLMNVLIPVSTALLLHEIVFNRLKRWMQTIIYLPHFLSWVI 120 Query: 121 LATVVMNIFSYEGPVNAFLGWLGIDPVLFMASNHWFRPMLVLTDVWKGFGYGSIIYLASL 180 LA ++ ++ S G VN + + +P+ F+ SN+WF ++VL+DVWK FG+ +II++A+L Sbjct: 121 LAGILTDMLSQNGIVNQVIKSITGEPIFFLGSNNWFPVVVVLSDVWKEFGFNTIIFIAAL 180 Query: 181 TAIDPGLYEASSIDGANRFKQLWHITLPGLMXXXXXXXXXXXXXXXXAGFDQIFNLYNPL 240 TAI+P LYEA+SIDGA R +++W +TLPG++ AGFDQI+NLYNPL Sbjct: 181 TAINPSLYEAASIDGAGRIRKIWSVTLPGILPTIILVSTLNIGNILNAGFDQIYNLYNPL 240 Query: 241 VYATSDIIDTYVYRVGLVERQYSLGTAIGLLRSVVGIVLILSANKLAQKLTDYRIF 296 VY + DIIDTYVYR+GLV+ QY L TA+G L+SVVG +LI + +LA + YRIF Sbjct: 241 VYESGDIIDTYVYRIGLVQYQYGLATAVGTLKSVVGFILIFVSYRLAARFAGYRIF 296 >C4ZHX8.1 C4ZHX8_9FIRM Putative uncharacterized protein Length = 296 Score = 307 bits (787), Expect = 7e-82, Method: Compositional matrix adjust. Identities = 147/293 (50%), Positives = 208/293 (70%) Query: 4 KKDQAAFHLMLAPSIVFLIIFSFIPMFGIVMAFQNYIPAKGISDSAWVGLDNFKFMFQIP 63 K+ +++ML P++++L FS +PMFGIVMAFQ+Y P +GI S +VGL NF++MFQ+ Sbjct: 4 KRFVKHYYIMLIPALLWLFFFSIVPMFGIVMAFQDYNPGQGILHSKFVGLANFEYMFQMS 63 Query: 64 DSRQILVNTIVIALWKIIVGTIVSIVFALLLNEIRVRFAKKFMQTVVYLPNFLSWAVLAT 123 D +Q+L NT+VIA+ KII I+ +VFALLLNE+ ++ K+ +QT+VYLP FLSW +LA Sbjct: 64 DIKQVLRNTVVIAVGKIIGNIIIPLVFALLLNELALKKLKRPIQTIVYLPYFLSWVILAK 123 Query: 124 VVMNIFSYEGPVNAFLGWLGIDPVLFMASNHWFRPMLVLTDVWKGFGYGSIIYLASLTAI 183 +V+NIF Y GPVN + + G + V F F+P+++ TD+WKGFGY +++YLA++ + Sbjct: 124 IVLNIFGYTGPVNQIIEFFGGEAVNFFGEAKIFQPLVIGTDIWKGFGYNTVVYLAAILGV 183 Query: 184 DPGLYEASSIDGANRFKQLWHITLPGLMXXXXXXXXXXXXXXXXAGFDQIFNLYNPLVYA 243 D LYEA+++DG RFK++WH+TLPG+ AGFDQ++NLYNPLVY+ Sbjct: 184 DGSLYEAAAVDGCGRFKRIWHVTLPGIKTTVALLAILSLGNILNAGFDQVYNLYNPLVYS 243 Query: 244 TSDIIDTYVYRVGLVERQYSLGTAIGLLRSVVGIVLILSANKLAQKLTDYRIF 296 T DIIDT+VYR GL QYSL TA+GL +SV+ +VLI LA K T Y++F Sbjct: 244 TGDIIDTWVYRAGLQNLQYSLATAVGLFKSVISLVLITVGYWLADKFTGYKLF 296 >B3KEX2.1 B3KEX2_9BACI Binding-protein-dependent transport systems inner membrane component Length = 296 Score = 304 bits (778), Expect = 7e-81, Method: Compositional matrix adjust. Identities = 147/296 (49%), Positives = 205/296 (69%) Query: 1 MNNKKDQAAFHLMLAPSIVFLIIFSFIPMFGIVMAFQNYIPAKGISDSAWVGLDNFKFMF 60 M K ++LML P ++L +FS +PMFGI+++FQ + P KGI S W G ++FK+M Sbjct: 1 MRMKGFAKHYYLMLIPGFLWLFVFSIVPMFGIIISFQYFNPGKGILGSDWAGFEHFKYML 60 Query: 61 QIPDSRQILVNTIVIALWKIIVGTIVSIVFALLLNEIRVRFAKKFMQTVVYLPNFLSWAV 120 + D++++ NT+VIA KI+ I ++FAL+LNE+R+ ++++QT+VYLP+FLSW + Sbjct: 61 LLKDTQEVFSNTLVIASMKIVANLIAPLLFALMLNELRLMILRRWIQTIVYLPHFLSWVI 120 Query: 121 LATVVMNIFSYEGPVNAFLGWLGIDPVLFMASNHWFRPMLVLTDVWKGFGYGSIIYLASL 180 L +V+++FSY GPVN G P LF A + F ++V +DVWK FG+G+IIYLA+L Sbjct: 121 LGGIVLDLFSYNGPVNQLTALFGFQPKLFFADSQLFPFLVVGSDVWKEFGFGTIIYLAAL 180 Query: 181 TAIDPGLYEASSIDGANRFKQLWHITLPGLMXXXXXXXXXXXXXXXXAGFDQIFNLYNPL 240 T I+P LYEA++IDGA R++ LWHITLPG+ AGFDQIFNLYNPL Sbjct: 181 TGINPALYEAANIDGAGRWRSLWHITLPGIRTTAVLLTVLSLGNVLNAGFDQIFNLYNPL 240 Query: 241 VYATSDIIDTYVYRVGLVERQYSLGTAIGLLRSVVGIVLILSANKLAQKLTDYRIF 296 VY+T DIIDT+VYR GL+ QY L TA+GLL+SVVG +LI + LA + +YRIF Sbjct: 241 VYSTGDIIDTWVYRTGLLNLQYELATAVGLLKSVVGFLLISVSYYLAYRFVNYRIF 296 >B1DFU4.1 B1DFU4_9BACL Binding-protein-dependent transport systems inner membrane component Length = 297 Score = 303 bits (777), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 148/297 (49%), Positives = 204/297 (68%), Gaps = 1/297 (0%) Query: 1 MNNKKDQAAFHLMLAPSIVFLIIFSFIPMFGIVMAFQNYIPAKGISDSAWVGLDNFKFMF 60 M K+ + LML P + L +FS PMFGI+MAFQ++ P KG+ S VG +NF +MF Sbjct: 1 MQLSKNSYHYLLMLIPGLALLALFSIAPMFGIIMAFQHFNPGKGMWHSPLVGFENFSYMF 60 Query: 61 QIPDSRQILVNTIVIALWKIIVGTIVSIVFALLLNEIRVRFAKKFMQTVVYLPNFLSWAV 120 S Q + NT++I++ K++ G IV +VFAL LNE+R F K+ +QT+VYLP+FLSW + Sbjct: 61 HNQASWQAIRNTLIISISKMVTGLIVPVVFALFLNEVRQMFFKRVVQTIVYLPHFLSWVI 120 Query: 121 LATVVMNIFSYEGPVNAFLGWLGI-DPVLFMASNHWFRPMLVLTDVWKGFGYGSIIYLAS 179 +A ++ +I G +N LG LG+ DP+LF+ SN WFRP+LV+TDVWK FG+ +I+YLA+ Sbjct: 121 IAGIIKDILGSNGLINVILGHLGVHDPILFLGSNTWFRPILVVTDVWKEFGFSAIVYLAA 180 Query: 180 LTAIDPGLYEASSIDGANRFKQLWHITLPGLMXXXXXXXXXXXXXXXXAGFDQIFNLYNP 239 LT I+P LYEA+ IDGA R +++ +I+LPG+ AGFDQ+FNLYNP Sbjct: 181 LTNINPSLYEAADIDGATRMQKIRYISLPGITTTVILLATLSLQGVLNAGFDQVFNLYNP 240 Query: 240 LVYATSDIIDTYVYRVGLVERQYSLGTAIGLLRSVVGIVLILSANKLAQKLTDYRIF 296 +V T DIIDTYVYR+G + Y LGTA+GL +SV+ ++LI +A KLA K YRIF Sbjct: 241 VVEPTGDIIDTYVYRIGFEQVNYELGTAVGLFKSVISLLLIATAYKLASKFAGYRIF 297 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.330 0.144 0.443 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,628,746,949 Number of extensions: 64085438 Number of successful extensions: 233766 Number of sequences better than 1.0e-05: 5040 Number of HSP's gapped: 228775 Number of HSP's successfully gapped: 5058 Length of query: 296 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 161 Effective length of database: 1,788,859,178 Effective search space: 288006327658 Effective search space used: 288006327658 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)