BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0443.0010.p (295 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D9Q7.1 B1D9Q7_9BACL Binding-protein-dependent transport system... 281 6e-74 B1D920.1 B1D920_9BACL Binding-protein-dependent transport system... 271 8e-71 Q9KDZ5.1 Q9KDZ5_BACHD BH1066 protein 263 1e-68 B3K5M7.1 B3K5M7_9BACI Binding-protein-dependent transport system... 259 2e-67 C3J6V4.1 C3J6V4_9BACI Binding-protein-dependent transport system... 258 4e-67 >B1D9Q7.1 B1D9Q7_9BACL Binding-protein-dependent transport systems inner membrane component Length = 292 Score = 281 bits (718), Expect = 6e-74, Method: Compositional matrix adjust. Identities = 144/286 (50%), Positives = 202/286 (70%), Gaps = 5/286 (1%) Query: 12 RIADTIIWILVLAFTLSCLIPLINLVAISFSDNAAASANMVSIFPVNFTLSSYEKLVSDS 71 R D ++ +++L TL CL+PL+N+V++SFS +AA++ V++FP +FT SYE + DS Sbjct: 10 RTTDIVLTLVMLLITLLCLLPLVNVVSLSFSGKSAAASGAVTLFPKDFTFYSYEYMFKDS 69 Query: 72 QFWRSFMISVERVVLGLIVNMMLMILAAYPLSKSSKQFRAQKVYMNIVIFAMLFSGGLIP 131 +F+ +F +SV+RV+LG +N +L I+ AYPL+K ++FR + +YM +++F M+F+GGLIP Sbjct: 70 RFFDAFGVSVKRVLLGGAINFVLTIMMAYPLAKERQEFRGRDIYMWVIVFTMMFTGGLIP 129 Query: 132 TFMVVKGLHLLDSIWALILPGAVPIANVILLMNAFRAVPKSLEEAATIDGASQWRILSTI 191 +MVVK LHL D+IWAL+LP AVPI NVILLMN FR +PK + E+A +DGA WR L TI Sbjct: 130 WYMVVKSLHLTDTIWALVLPTAVPIFNVILLMNFFRGMPKEINESAKMDGAGPWRTLITI 189 Query: 192 YXXXXXXXXXXXXXXXIVGHWNDYFSALVYINKTSNYPLQTYIQQLSV--DVQNITDPQK 249 IV HWN +F L+ +NK N PLQTYIQQL V + + T P+ Sbjct: 190 AVPLAMPAIATVTLFSIVSHWNAFFDGLILMNKQHNIPLQTYIQQLVVAPSITSSTRPED 249 Query: 250 LIEYSKISNRTLNAAKIVISTLPLLLIYPFMQKYFVTGIVVGSVKE 295 LI +S+ RT NAAKIV++ LP+L+IYPF+Q+YFV GI +GSVKE Sbjct: 250 LINFSQ---RTFNAAKIVVTMLPILIIYPFLQRYFVHGITLGSVKE 292 >B1D920.1 B1D920_9BACL Binding-protein-dependent transport systems inner membrane component Length = 295 Score = 271 bits (692), Expect = 8e-71, Method: Compositional matrix adjust. Identities = 128/290 (44%), Positives = 201/290 (69%), Gaps = 2/290 (0%) Query: 8 SVKSRIADTIIWILVLAFTLSCLIPLINLVAISFSDNAAASANMVSIFPVNFTLSSYEKL 67 ++ SR D IL+ +SC+ PL +S S+ +AA+A +V+I+P+ F+L+ Y+++ Sbjct: 6 TLGSRAFDIANIILLGLLVISCVYPLWYTFCVSISEKSAANAGLVTIYPIGFSLTPYKEI 65 Query: 68 VSDSQFWRSFMISVERVVLGLIVNMMLMILAAYPLSKSSKQFRAQKVYMNIVIFAMLFSG 127 ++DS F+ +F IS++R VLG +++ +L AYPL++ K F+ + ++M I++F MLFSG Sbjct: 66 INDSMFFNAFWISIQRTVLGTSFALLMTVLMAYPLARPQKDFKTRNIFMWILVFCMLFSG 125 Query: 128 GLIPTFMVVKGLHLLDSIWALILPGAVPIANVILLMNAFRAVPKSLEEAATIDGASQWRI 187 GLIP ++ ++ HL+DSIWAL+L G VP+ +VIL+MN FR +PK L EAA +DGA W + Sbjct: 126 GLIPWYLTIQNYHLIDSIWALVLGGGVPVFDVILIMNFFRNLPKELNEAAVVDGAGPWSV 185 Query: 188 LSTIYXXXXXXXXXXXXXXXIVGHWNDYFSALVYINKTSNYPLQTYIQQLSVDVQNITD- 246 L +Y V HWN++F+ LV N S+YPLQTYIQQL V++ T+ Sbjct: 186 LFRVYVPCSIPVLAAVALFISVYHWNEFFNGLVLSNTQSSYPLQTYIQQLVVNIPVGTNL 245 Query: 247 -PQKLIEYSKISNRTLNAAKIVISTLPLLLIYPFMQKYFVTGIVVGSVKE 295 P++ + S++SNRTLN+AK+ I+ +P+L++YPF+QKYFV+GI++G+VKE Sbjct: 246 TPEQYKKLSELSNRTLNSAKVFIAMVPMLVVYPFLQKYFVSGIMLGAVKE 295 >Q9KDZ5.1 Q9KDZ5_BACHD BH1066 protein Length = 293 Score = 263 bits (673), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 131/280 (46%), Positives = 190/280 (67%), Gaps = 6/280 (2%) Query: 19 WILVLAFTLSCLIPLINLVAISFSDNAAASANMVSIFPVNFTLSSYEKLVSDSQFWRSFM 78 + +L F + C++PL+++ A+S S + AA+AN+V PV+FTL +YE+ +++ F RSF+ Sbjct: 17 YTFLLLFAVLCILPLVHIFAVSLSGSGAAAANLVMFLPVDFTLDAYERTIANDNFIRSFL 76 Query: 79 ISVERVVLGLIVNMMLMILAAYPLSKSSKQFRAQKVYMNIVIFAMLFSGGLIPTFMVVKG 138 ISV RVVLG +++M M+ AAY LSK + F+ +K Y+ + MLF+GGL+PT+MVV G Sbjct: 77 ISVVRVVLGTVISMGAMLCAAYALSKEDRDFKGRKFYIWFFVITMLFNGGLVPTYMVVTG 136 Query: 139 LHLLDSIWALILPGAVPIANVILLMNAFR-AVPKSLEEAATIDGASQWRILSTIYXXXXX 197 L L ++IWAL+LP A+ N+ILL+N FR AVPKSLEE+A IDGA+ ++I IY Sbjct: 137 LGLTNTIWALVLPPAINAFNLILLLNFFRTAVPKSLEESAFIDGANHFQIFLKIYLPISV 196 Query: 198 XXXXXXXXXXIVGHWNDYFSALVYINKTSNYPLQTYIQQLSV--DVQNITDPQKLIEYSK 255 +V HWN +F ++Y N NYPLQT++Q + V D ITD + L Sbjct: 197 PAIATVSLFTMVFHWNSWFDGMIYNNDARNYPLQTFLQTIVVQQDFSQITDAETL---RN 253 Query: 256 ISNRTLNAAKIVISTLPLLLIYPFMQKYFVTGIVVGSVKE 295 +S RT+ AA+I I+ LP+L++YPF+QKYFV GIV+G+ KE Sbjct: 254 LSQRTVQAAQIFIAALPMLIVYPFIQKYFVKGIVLGAEKE 293 >B3K5M7.1 B3K5M7_9BACI Binding-protein-dependent transport systems inner membrane component Length = 295 Score = 259 bits (663), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 129/273 (47%), Positives = 179/273 (65%), Gaps = 3/273 (1%) Query: 26 TLSCLIPLINLVAISFSDNAAASANMVSIFPVNFTLSSYEKLVSDSQFWRSFMISVERVV 85 TL +IP ++L++IS S N AA A V ++PV F L +Y L +F+RSF +S+ RVV Sbjct: 23 TLLGIIPFVHLLSISLSSNTAAMAGEVRLWPVGFNLDAYRYLGEKVEFFRSFRVSLTRVV 82 Query: 86 LGLIVNMMLMILAAYPLSKSSKQFRAQKVYMNIVIFAMLFSGGLIPTFMVVKGLHLLDSI 145 LG VNM+L+ + AYPLSKS+ QF+ + Y+ M F GGLIPT+MVVK L+DS+ Sbjct: 83 LGTAVNMLLVFITAYPLSKSNHQFKFRTPYVWFFAITMFFGGGLIPTYMVVKNTGLIDSV 142 Query: 146 WALILPGAVPIANVILLMNAFRAVPKSLEEAATIDGASQWRILSTIYXXXXXXXXXXXXX 205 WALILPGA+ + N++L++N FR +PK LEEAATIDGA WRIL IY Sbjct: 143 WALILPGALNVWNMVLMLNFFRTIPKELEEAATIDGAGHWRILWRIYLPVSLPSIATIGL 202 Query: 206 XXIVGHWNDYFSALVYINKTSNYPLQTYIQQL--SVDVQNIT-DPQKLIEYSKISNRTLN 262 IVGHWN +F ++Y+N YPLQTY+ L S++ Q + +++ +S +TL Sbjct: 203 FTIVGHWNAWFDGILYLNSPEKYPLQTYLSTLIMSINSQMTSISIEQIKAMENLSEKTLR 262 Query: 263 AAKIVISTLPLLLIYPFMQKYFVTGIVVGSVKE 295 A+I + LP++++YPF+QKYFV G+ VGSVKE Sbjct: 263 TAQIFMGALPIMVVYPFLQKYFVKGMTVGSVKE 295 >C3J6V4.1 C3J6V4_9BACI Binding-protein-dependent transport systems inner membrane component Length = 296 Score = 258 bits (660), Expect = 4e-67, Method: Compositional matrix adjust. Identities = 133/272 (48%), Positives = 187/272 (68%), Gaps = 9/272 (3%) Query: 29 CLIPLINLVAISFSDNAAASANMVSIFPVNFTLSSYEKLVSDSQFWRSFMISVERVVLGL 88 CL+PLI+++A+SFS +AAA+AN+V ++PV+FTL +YEK + + F RS S RVVLG Sbjct: 29 CLLPLIHILAVSFSGSAAATANLVGLWPVDFTLDAYEKTIGNENFLRSLWNSFLRVVLGT 88 Query: 89 IVNMMLMILAAYPLSKSSKQFRAQKVYMNIVIFAMLFSGGLIPTFMVVKGLHLLDSIWAL 148 +++M +M+ AAYPLSKS FR +K+YM + +F MLF GGL+PT+MVV L L D+IWAL Sbjct: 89 VISMGVMLCAAYPLSKSDDAFRGRKLYMWVFVFTMLFHGGLVPTYMVVTKLGLTDTIWAL 148 Query: 149 ILPGAVPIANVILLMNAFR-AVPKSLEEAATIDGASQWRILSTIYXXXXXXXXXXXXXXX 207 ILP AV N+ILL+N FR +VPKSLEEAA +DGA+ + I IY Sbjct: 149 ILPPAVSAFNLILLVNFFRTSVPKSLEEAALMDGANHFTIFWRIYLPISVPAIATISLFT 208 Query: 208 IVGHWNDYFSALVYINKTSNYPLQTYIQQLSVDVQNIT----DPQKLIEYSKISNRTLNA 263 +V HWN +F L+Y++ +YPL T++Q + V Q+ T D + L +S RT+ A Sbjct: 209 MVFHWNSWFDGLIYMSDVKHYPLSTFLQTIIVQ-QDFTKMSVDAETL---KNLSERTVKA 264 Query: 264 AKIVISTLPLLLIYPFMQKYFVTGIVVGSVKE 295 A+I I+ LP+L++YPF+QK+FV GIV+G+ KE Sbjct: 265 AQIFIAALPMLIVYPFVQKFFVKGIVLGAEKE 296 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.138 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,429,187,686 Number of extensions: 48206256 Number of successful extensions: 211229 Number of sequences better than 1.0e-05: 4821 Number of HSP's gapped: 204918 Number of HSP's successfully gapped: 4827 Length of query: 295 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 161 Effective length of database: 1,798,280,193 Effective search space: 289523111073 Effective search space used: 289523111073 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 131 (55.1 bits)