BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0443.0009.p (748 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C2IT68.1 C2IT68_VIBCH Glycogen debranching enzyme 359 5e-97 A2P3Q0.1 A2P3Q0_VIBCH Glycogen debranching enzyme 358 1e-96 B8K4W5.1 B8K4W5_VIBPA Glycogen debranching enzyme 327 3e-87 Q6LK90.1 Q6LK90_PHOPR Hypothetical glycogen debranching enzyme 326 5e-87 Q1Z0F1.1 Q1Z0F1_PHOPR Hypothetical glycogen debranching enzyme 323 4e-86 >C2IT68.1 C2IT68_VIBCH Glycogen debranching enzyme Length = 796 Score = 359 bits (922), Expect = 5e-97, Method: Compositional matrix adjust. Identities = 181/413 (43%), Positives = 257/413 (62%), Gaps = 9/413 (2%) Query: 235 WLNITMDQLVTRQQG-DGIYAGLPWFNEYWGRDQFIALPGAVLVTGQFETAKNILLSFAK 293 W + ++ ++ G GI+AGLPWF + WGRD FIALPG LV G F TAK+I+ FA+ Sbjct: 270 WARFSGHSMINQEFGYSGIWAGLPWFKDGWGRDTFIALPGICLVNGDFATAKSIIEDFAR 329 Query: 294 FQNTDAASKYFGRVPN-ILALKNVDYHTTDGTPRFIIQLQDYVKYSGDTAVITELYPAVQ 352 Q T S ++GR+PN + + + Y+TTDGTP I +L +Y+ YSGDTA ++LYP ++ Sbjct: 330 LQETRPDSVHYGRIPNRVSSDSQIIYNTTDGTPWMIRELLEYLNYSGDTAFASKLYPVLK 389 Query: 353 NSIEGSIKHWVDDKGYLMHDDNETWMDARDANLKSYSPRDTRANDIQALWYNQLQAGVYF 412 + I G +H++ D G ++H D +TWMDA+ +SPR +ANDIQALWY LQ GV Sbjct: 390 HFISGVERHYLADDGCMLHRDPDTWMDAKIEGQIPWSPRGPKANDIQALWYESLQVGVTL 449 Query: 413 AEYMGDSGSAKKWGNIASKLKRNFAADFRDQTHPYLADRLNPEDEPEFSLRPNQLFALDM 472 A+ +GD+ SA W +A K+K++F F D + LADRL+ D P++++RPNQL + + Sbjct: 450 AQLLGDATSADHWQCMAQKVKQSFTEKFWDSSKLRLADRLDHTDHPDWTIRPNQLMTITI 509 Query: 473 -----IDDDSFKCEVIKTAWEELVYPWGVASLDQKHPFFHPFHLT-SLYHKDEAYHNGTV 526 + + + ++ A E L++PWG+ SL+Q HP FHP+H S YHKD AYHNGT+ Sbjct: 510 PQQQPLFSEEYAAAILTQATENLLFPWGICSLNQHHPEFHPYHDNRSEYHKDAAYHNGTI 569 Query: 527 WLWLNGIAMQRMIEAGQEETAYKLFKNMNWQALNLGVVGGLSENMDAYPQEGQSWAKLTG 586 W W G + + Q E AY+L K + Q L+ G G +SEN+DAY + + +G Sbjct: 570 WGWNAGFTISALTRFNQTELAYQLTKQLTHQVLHQGHRGTMSENLDAYQISTEKLVE-SG 628 Query: 587 AYLQAWSNAEQLRVWYQYFLGFRPDMIHQALTLAPRIPEEIRDLQARVKIGSG 639 Y QAWS +E R Q +LG+RP M+ Q LTLAPR P E ++ ARV GS Sbjct: 629 TYSQAWSVSEFARNAQQDYLGYRPQMLQQTLTLAPRFPNEWNEVSARVPFGSS 681 >A2P3Q0.1 A2P3Q0_VIBCH Glycogen debranching enzyme Length = 772 Score = 358 bits (919), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 181/413 (43%), Positives = 256/413 (61%), Gaps = 9/413 (2%) Query: 235 WLNITMDQLVTRQQG-DGIYAGLPWFNEYWGRDQFIALPGAVLVTGQFETAKNILLSFAK 293 W + ++ ++ G GI+AGLPWF + WGRD FIALPG LV G F TAK+I+ FA+ Sbjct: 246 WARFSGHSMINQEFGYSGIWAGLPWFKDGWGRDTFIALPGICLVNGDFATAKSIIEDFAR 305 Query: 294 FQNTDAASKYFGRVPN-ILALKNVDYHTTDGTPRFIIQLQDYVKYSGDTAVITELYPAVQ 352 Q T S ++GR+PN + + + Y+TTDGTP I +L +Y+ YSGDTA ++LYP ++ Sbjct: 306 LQETRPDSVHYGRIPNRVSSDSQIIYNTTDGTPWMIRELLEYLNYSGDTAFASKLYPVLK 365 Query: 353 NSIEGSIKHWVDDKGYLMHDDNETWMDARDANLKSYSPRDTRANDIQALWYNQLQAGVYF 412 + I G +H++ D G ++H D +TWMDA+ +SPR +ANDIQALWY LQ GV Sbjct: 366 HFISGVERHYLADDGCMLHRDPDTWMDAKIEGQIPWSPRGPKANDIQALWYESLQVGVTL 425 Query: 413 AEYMGDSGSAKKWGNIASKLKRNFAADFRDQTHPYLADRLNPEDEPEFSLRPNQLFALDM 472 A+ +GD+ SA W +A K+K++F F D + LADRL+ D P++++RPNQL + + Sbjct: 426 AQLLGDATSADHWQCMAQKVKQSFTEKFWDSSKLRLADRLDHTDHPDWTIRPNQLMTITI 485 Query: 473 IDD-----DSFKCEVIKTAWEELVYPWGVASLDQKHPFFHPFHLT-SLYHKDEAYHNGTV 526 + + ++ A E L++PWG+ SL+Q HP FHP+H S YHKD AYHNGT+ Sbjct: 486 PQQQPLFIEEYAAAILTQATENLLFPWGICSLNQHHPEFHPYHDNRSEYHKDAAYHNGTI 545 Query: 527 WLWLNGIAMQRMIEAGQEETAYKLFKNMNWQALNLGVVGGLSENMDAYPQEGQSWAKLTG 586 W W G + + Q E AY+L K + Q L+ G G +SEN+DAY + + +G Sbjct: 546 WGWNAGFTISALTRFNQTELAYQLTKQLTHQVLHQGHRGTMSENLDAYQISTEKLVE-SG 604 Query: 587 AYLQAWSNAEQLRVWYQYFLGFRPDMIHQALTLAPRIPEEIRDLQARVKIGSG 639 Y QAWS +E R Q +LG+RP M+ Q LTLAPR P E ++ ARV GS Sbjct: 605 TYSQAWSVSEFARNAQQDYLGYRPQMLQQTLTLAPRFPNEWNEVSARVPFGSS 657 >B8K4W5.1 B8K4W5_VIBPA Glycogen debranching enzyme Length = 923 Score = 327 bits (838), Expect = 3e-87, Method: Compositional matrix adjust. Identities = 169/410 (41%), Positives = 241/410 (58%), Gaps = 8/410 (1%) Query: 235 WLNITMDQLVTRQQGDGIYAGLPWFNEYWGRDQFIALPGAVLVTGQFETAKNILLSFAKF 294 W ++ V+ + G GI+AGLPWF + WGRD FIAL G LV G+FE AK+I+ +FA Sbjct: 394 WARLSSRVFVSHEFGTGIWAGLPWFKDCWGRDTFIALSGTSLVNGEFEEAKSIIENFASM 453 Query: 295 QNTDAASKYFGRVPNILALK-NVDYHTTDGTPRFIIQLQDYVKYSGDTAVITELYPAVQN 353 Q D S +GR+PN + K N+ Y+TTDGTP + ++ +Y+ YSGD A +YP +Q Sbjct: 454 QQLDTQSVNYGRIPNRVTSKTNIIYNTTDGTPWMMREILEYINYSGDRAFAKRIYPVIQR 513 Query: 354 SIEGSIKHWVDDKGYLMHDDNETWMDARDANLKSYSPRDTRANDIQALWYNQLQAGVYFA 413 I G H++D G + H +TWMDA+ +SPR +ANDIQALW+ L A Sbjct: 514 FISGVETHYLDADGLMKHRHPDTWMDAKINGQIPWSPRGPKANDIQALWFESLNVAKQVA 573 Query: 414 EYMGDSGSAKKWGNIASKLKRNFAADFRDQTHPYLADRLNPEDEPEFSLRPNQLFALDMI 473 D A+ +A K++F F D+ H LADRL D P+FS+RPNQL A+ + Sbjct: 574 LLNNDQTFAEHVDTLAKHAKQSFIDKFWDEEHLQLADRLEENDRPDFSVRPNQLMAMTIP 633 Query: 474 DDDSFKC-----EVIKTAWEELVYPWGVASLDQKHPFFHPFHLT-SLYHKDEAYHNGTVW 527 +S ++K + L++PWG+ SL+Q+HP FHP+H S YHKD AYHNGT+W Sbjct: 634 QQESLIAPSIGQHMVKNCVDNLLFPWGICSLNQQHPDFHPYHDNRSEYHKDAAYHNGTIW 693 Query: 528 LWLNGIAMQRMIEAGQEETAYKLFKNMNWQALNLGVVGGLSENMDAYPQEGQSWAKLTGA 587 W G + + Q++ AY+L KN+ Q L G G +SEN+DA+ Q + TG Sbjct: 694 GWNAGFTVSALNRYHQQDFAYQLSKNLAKQILQQGHRGTMSENLDAFQTHPQQLVE-TGT 752 Query: 588 YLQAWSNAEQLRVWYQYFLGFRPDMIHQALTLAPRIPEEIRDLQARVKIG 637 Y QAWS +E R Q +LGF+P +I ++ +AP+IP + + +AR+ G Sbjct: 753 YAQAWSVSEFARNAQQDYLGFKPKLIANSIEIAPKIPSQWQSFKARLPFG 802 >Q6LK90.1 Q6LK90_PHOPR Hypothetical glycogen debranching enzyme Length = 931 Score = 326 bits (836), Expect = 5e-87, Method: Compositional matrix adjust. Identities = 172/411 (41%), Positives = 244/411 (59%), Gaps = 8/411 (1%) Query: 235 WLNITMDQLVTRQQGDGIYAGLPWFNEYWGRDQFIALPGAVLVTGQFETAKNILLSFAKF 294 W + V+ + G GI+AGLPWF + WGRD FIALPG LV G F+ AK I+ +FA Sbjct: 401 WAKASGKVFVSNEFGKGIWAGLPWFKDCWGRDSFIALPGITLVNGDFDDAKTIIDNFASM 460 Query: 295 QNTDAASKYFGRVPN-ILALKNVDYHTTDGTPRFIIQLQDYVKYSGDTAVITELYPAVQN 353 Q TD S +GR+PN + +L N+ Y+TTDGTP + ++ DY++YSGD +YP VQ Sbjct: 461 QLTDDQSLNYGRIPNRVTSLTNIIYNTTDGTPWMVREVWDYLRYSGDLDYAKTIYPIVQT 520 Query: 354 SIEGSIKHWVDDKGYLMHDDNETWMDARDANLKSYSPRDTRANDIQALWYNQLQAGVYFA 413 I+G K+++D G + H D +TWMDA+ +S R RANDIQALWY L+ + A Sbjct: 521 YIDGIEKNYLDQHGLMTHRDPDTWMDAKLEGKVPWSARGNRANDIQALWYTSLKVAIELA 580 Query: 414 EYMGDSGSAKKWGNIASKLKRNFAADFRDQTHPYLADRLNPEDEPEFSLRPNQLFALDM- 472 D SA ++ +A K++ +F F D+ LADRL +D + +RPNQL L + Sbjct: 581 RLNQDQASALRYRAMADKVQFSFQKMFWDEQTEMLADRLKEDDSRDLQVRPNQLMTLTVP 640 Query: 473 ----IDDDSFKCEVIKTAWEELVYPWGVASLDQKHPFFHPFHLT-SLYHKDEAYHNGTVW 527 + D +V+K A EL+YPWG+ SL Q P FHPFH YHKD AYHNGT+W Sbjct: 641 FDGGLVDKLIGAKVVKNAVSELLYPWGITSLSQYDPDFHPFHANRDEYHKDAAYHNGTIW 700 Query: 528 LWLNGIAMQRMIEAGQEETAYKLFKNMNWQALNLGVVGGLSENMDAYPQEGQSWAKLTGA 587 W G + +++ G + AY+L KN++ Q L++G G +SEN+DA+ TG Sbjct: 701 GWNAGFTVSSLVKYGYADFAYELTKNLSQQILSIGHRGAMSENLDAFLSTEGG-LTTTGT 759 Query: 588 YLQAWSNAEQLRVWYQYFLGFRPDMIHQALTLAPRIPEEIRDLQARVKIGS 638 Y QAWS +E R YQ ++GF P+++ +TLAP +P + QA ++ G+ Sbjct: 760 YAQAWSVSEFTRNGYQDYMGFSPNLLADKVTLAPCLPSSWQSAQAVLRFGN 810 >Q1Z0F1.1 Q1Z0F1_PHOPR Hypothetical glycogen debranching enzyme Length = 931 Score = 323 bits (828), Expect = 4e-86, Method: Compositional matrix adjust. Identities = 172/411 (41%), Positives = 242/411 (58%), Gaps = 8/411 (1%) Query: 235 WLNITMDQLVTRQQGDGIYAGLPWFNEYWGRDQFIALPGAVLVTGQFETAKNILLSFAKF 294 W + V+ + G GI+AGLPWF + WGRD FIALPG LV G F+ AK I+ +FA Sbjct: 401 WAKASGKVFVSNEFGKGIWAGLPWFKDCWGRDSFIALPGITLVNGDFDDAKTIIDNFASM 460 Query: 295 QNTDAASKYFGRVPN-ILALKNVDYHTTDGTPRFIIQLQDYVKYSGDTAVITELYPAVQN 353 Q TD S +GR+PN + +L N+ Y+TTDGTP + ++ DY++YSGD +YP VQ Sbjct: 461 QLTDDQSLNYGRIPNRVTSLTNIIYNTTDGTPWMVREVWDYLRYSGDLDYAKTIYPIVQT 520 Query: 354 SIEGSIKHWVDDKGYLMHDDNETWMDARDANLKSYSPRDTRANDIQALWYNQLQAGVYFA 413 I+G K+++D G + H D +TWMDA+ +S R RANDIQALWY L+ + A Sbjct: 521 YIDGIEKNYLDQYGLMTHRDPDTWMDAKLEGKVPWSARGNRANDIQALWYTSLKVAIELA 580 Query: 414 EYMGDSGSAKKWGNIASKLKRNFAADFRDQTHPYLADRLNPEDEPEFSLRPNQLFALDM- 472 D SA ++ +A K++ +F F D+ LADRL +D + +RPNQL L + Sbjct: 581 RLNQDQASALRYRAMADKVQFSFQKMFWDEQTEMLADRLKEDDSRDLQVRPNQLMTLTVP 640 Query: 473 ----IDDDSFKCEVIKTAWEELVYPWGVASLDQKHPFFHPFHLT-SLYHKDEAYHNGTVW 527 + D +V+K A EL+YPWG+ SL Q P FHPFH YHKD AYHNGT+W Sbjct: 641 FDGGLVDKLIGAKVVKNAVSELLYPWGITSLSQYDPDFHPFHANRDEYHKDAAYHNGTIW 700 Query: 528 LWLNGIAMQRMIEAGQEETAYKLFKNMNWQALNLGVVGGLSENMDAYPQEGQSWAKLTGA 587 W G + +++ G + AY+L KN++ Q L++G G +SEN+DA+ TG Sbjct: 701 GWNAGFTVSSLVKYGYADFAYELTKNLSQQILSIGHRGAMSENLDAFLSTEGG-LTTTGT 759 Query: 588 YLQAWSNAEQLRVWYQYFLGFRPDMIHQALTLAPRIPEEIRDLQARVKIGS 638 Y QAWS +E R YQ ++GF P+++ +TLAP +P QA + G+ Sbjct: 760 YAQAWSVSEFTRNGYQDYMGFSPNLLADKVTLAPCLPSSWLSAQAVLCFGN 810 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 4,387,041,436 Number of extensions: 183453070 Number of successful extensions: 411777 Number of sequences better than 1.0e-05: 258 Number of HSP's gapped: 411161 Number of HSP's successfully gapped: 261 Length of query: 748 Length of database: 3,060,696,203 Length adjustment: 144 Effective length of query: 604 Effective length of database: 1,704,070,043 Effective search space: 1029258305972 Effective search space used: 1029258305972 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 135 (56.6 bits)