BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0443.0001.p (603 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K5B7.1 B3K5B7_9BACI Transglutaminase domain protein 251 2e-64 B1D8V2.1 B1D8V2_9BACL Transglutaminase domain protein 200 4e-49 C2UUL2.1 C2UUL2_BACCE Membrane-bound protease 169 7e-40 C2VAM0.1 C2VAM0_BACCE Membrane-bound protease 166 5e-39 B7HPA0.1 B7HPA0_BACC7 Putative membrane-bound protease 165 1e-38 >B3K5B7.1 B3K5B7_9BACI Transglutaminase domain protein Length = 774 Score = 251 bits (640), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 193/582 (33%), Positives = 267/582 (45%), Gaps = 43/582 (7%) Query: 35 LHYRLLFSLAIMGLFMEWLLPLLRTAVEPDTLRLLHTLLFCAAALLLWGSLRLPNFVQYP 94 L +R+L +L +MGL EWLLPL R A E +L TL A LLL G +V P Sbjct: 38 LLHRILITLPVMGLLSEWLLPL-RNAGETGGRFVLETLFLWALFLLLQGLFTWRGWVWLP 96 Query: 95 VQFMIMSLTWFYICDGNKGGRWLSLYAAE--GSDDLALLFSGHISALSENSRXXXXXXXX 152 + ++ + + + WLS YA+ D A + + LS+ +R Sbjct: 97 LNAGLVVWLCSRLFEYSDPLAWLSDYASLILPGDASAFRNAWEFTILSQETRTIILLVGW 156 Query: 153 XXXXCSVQQLALYRGSTTLFTLVTLIYLLALDMGFTVKTTGDVIVALGXXXXXXXXXXXX 212 +V LALYR + LF TL+YL L+ GD++ A+ Sbjct: 157 GIMVSAVHMLALYRRTVWLFGGSTLVYLAVLESAMEESVYGDMLCAVLYMLLAQGMMLIL 216 Query: 213 XXK----EGTGS-------------AALPYTRXXXXXXXXXXXXXXXXXXGGQLYGARQG 255 K EGT S + LP R GG L G Sbjct: 217 KLKQETVEGTSSEMDLPEPMRTGRLSGLPLLRWSLFVLLASVLIAGITRVGGGLGEPSPG 276 Query: 256 GPITFQPILDRMQHWAAXXXXXXXXXXXXXXXXXXXXXXEAELGAPLASSTEAVFSVVAD 315 ++ + +R+ W E +LG PL S F+ + Sbjct: 277 MGLSVAEMAERLAGWGERLRGNTEPSIPASQVAGYASQGE-DLGGPLTLSDALYFTAQSP 335 Query: 316 RPSYWRGESIAYYDGRRWIRDGAAFLPFNLTA-----LPGPGQNQAEEAGKSRRLVQRIQ 370 P+YWRG++ YDGR+W D + P L LP + E +S VQ + Sbjct: 336 VPTYWRGDTYNRYDGRKWDTDPSIRKPVKLLEDLSGFLPYWERPSGETIVQSLTFVQ--E 393 Query: 371 FAVPSSGGLPLFGAGTVADIENITLTDGSRLGYVLANPQKDSFRLPEA---GGSVRVAEY 427 VPS L G + ++ + + P+ RL E G V+ Y Sbjct: 394 TPVPS-----LLSGGIITRVKEMHSSKKR------GAPRFAVDRLSETISLQGERAVSGY 442 Query: 428 TVASILPESDPAVLRASAGMDPVSVRDSYLQLPAQLPRRVAELAGRLTASADTRYAAVSA 487 TV ++ + + LR + G DP + + YL+LP +LP RV +LA ++T+ A RY A Sbjct: 443 TVETLYVQPEEQALRRANGDDPEYMEEMYLELPEELPSRVKDLARKITSDAAGRYETAQA 502 Query: 488 VQDYLQNGFTYTLKTRIPPQGSDFTDDFLFVTKQGYCVHFATAMTVLLRSSGIPARYVQG 547 ++ YL+ +TYTL+T +PP+G DFTD FLF K+GYCVHFATAMTVLLRS GIPARYV G Sbjct: 503 IESYLEQNYTYTLQTSVPPEGRDFTDHFLFDAKEGYCVHFATAMTVLLRSEGIPARYVTG 562 Query: 548 YGPGTLAADAVPPRYDVTQGDAHAWVEVYFPGTGWVPFDPTP 589 + PG A + RY+V Q +AHAWVEV+FPG GWV FDPTP Sbjct: 563 FAPGDRVAGTM-DRYEVAQKNAHAWVEVFFPGQGWVLFDPTP 603 >B1D8V2.1 B1D8V2_9BACL Transglutaminase domain protein Length = 850 Score = 200 bits (508), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 132/321 (41%), Positives = 173/321 (53%), Gaps = 37/321 (11%) Query: 297 ELGAPLASSTEAVFSVVADRPSYWRGESIAYYDGRRWIRD--GAAFLPFNLTALPGPGQN 354 ELGA L+ VFS + +YWRGES A Y+GR W G P G Sbjct: 286 ELGASLSQDDTIVFSGFSPVQTYWRGESKAVYNGRGWDSSWSGKVLHPVEY------GDK 339 Query: 355 QAEEA----GKSRRLVQRIQFAVPS-SGGLPLFGAGTVADIENITLTDGSRLGYVLANPQ 409 +E A G +R + Q + PS S GLPLF AG+ + +++ D +R L+ Sbjct: 340 LSELAVGTSGDNRTIRQTVVMTEPSASQGLPLFFAGSGGRVLSLSAIDPNRQ---LSTYV 396 Query: 410 KDSFR--LPEAGGSVRVAEYTVASILPESDPAVLRAS---------AGMDPVSVRD--SY 456 +D L A +V YTV S LP +D ++LR + A + D Y Sbjct: 397 EDELAGALYSADKEAQVERYTVESRLPLTDTSLLRETDSSAFAGETAALTQTEETDLSDY 456 Query: 457 LQLPAQLPRRVAELAGRLTASADT-RYAAVSAVQDYLQNGFTYTLKTRIPPQGSDFTDDF 515 LQLP LP RV LA ++ T RY V AV+ YL+N +TY+L + +PP+G+DF D F Sbjct: 457 LQLPDSLPDRVKTLADKIVGDERTDRYDQVKAVEQYLKNSYTYSLDSAVPPKGADFVDYF 516 Query: 516 LFVTKQGYCVHFATAMTVLLRSSGIPARYVQGYGPGTLAADA-------VPPRYDVTQGD 568 LF ++GYCVHF++AM VLLR GIPAR+V+G+ GT + A Y+V D Sbjct: 517 LFEQERGYCVHFSSAMVVLLREEGIPARWVKGFVSGTPVSTAGADVTSTAGTLYNVRAKD 576 Query: 569 AHAWVEVYFPGTGWVPFDPTP 589 AHAWVEVYFPG GWVPFDPTP Sbjct: 577 AHAWVEVYFPGAGWVPFDPTP 597 >C2UUL2.1 C2UUL2_BACCE Membrane-bound protease Length = 731 Score = 169 bits (428), Expect = 7e-40, Method: Compositional matrix adjust. Identities = 104/308 (33%), Positives = 150/308 (48%), Gaps = 29/308 (9%) Query: 295 EAELGAPLASSTEAVFSVVADRPSYWRGESIAYYDGRRW----IRDGAAFLPFNLTALPG 350 +++LG P + VF+ YWR E+ +Y G+ W +F N L Sbjct: 245 DSQLGGPFQADPTIVFTAQTQNKQYWRVETKDFYTGKGWEASEKLKKVSFKTKN-DVLSW 303 Query: 351 PGQNQAEEAGKSRRLVQRIQFAVPSSGGLPLFGAGTVADIENITLTDGSRLGYVLANPQK 410 QN E ++ +Q+ F + GL +++ S + Y + +P Sbjct: 304 YEQNTKTETTEATITMQKSDFHLTYPAGL-------------VSVETSSDVSYSV-DPIS 349 Query: 411 DSFRLPEAGGSVRVAEYTVASILPE---SDPAVLRASAGMDPVS-VRDSYLQLPAQLPRR 466 + G S + Y V +PE ++ + G + S Y QLP LP+R Sbjct: 350 EKIYTMHGGSSTTLNSYKVTYEIPEFPIESLKTVKTNEGHEASSYFMTKYTQLPESLPQR 409 Query: 467 VAELAGRLTASADTRYAAVSAVQDYL-QNGFTY-TLKTRIPPQGSDFTDDFLFVTKQGYC 524 V +LA LT D RY V A+++Y N F Y T P + D+ D FLF TK GYC Sbjct: 410 VKDLAVNLTNDKDNRYDKVLAIENYFTDNSFVYETTNVSFPAKSQDYVDQFLFDTKSGYC 469 Query: 525 VHFATAMTVLLRSSGIPARYVQGYGPG----TLAADAVPPRYDVTQGDAHAWVEVYFPGT 580 +F+T+M VLLRS+GIPAR+V+GY G TLA+ Y +T +AH+WVEVYFPG Sbjct: 470 NNFSTSMIVLLRSAGIPARWVKGYTEGTLDNTLASAEGQDIYKITNDNAHSWVEVYFPGY 529 Query: 581 GWVPFDPT 588 GW+PF+PT Sbjct: 530 GWIPFEPT 537 >C2VAM0.1 C2VAM0_BACCE Membrane-bound protease Length = 731 Score = 166 bits (421), Expect = 5e-39, Method: Compositional matrix adjust. Identities = 101/307 (32%), Positives = 148/307 (48%), Gaps = 27/307 (8%) Query: 295 EAELGAPLASSTEAVFSVVADRPSYWRGESIAYYDGRRW-IRDGAAFLPFNLT--ALPGP 351 ++ LG P + VF+ YWR E+ +Y G+ W + + + F + Sbjct: 245 DSRLGGPFQADPTIVFTAQTQNKQYWRVETKDFYTGKGWEVSENQKKISFKSKNDVVSWY 304 Query: 352 GQNQAEEAGKSRRLVQRIQFAVPSSGGLPLFGAGTVADIENITLTDGSRLGYVLANPQKD 411 QN E ++ +Q+ F + GL +++ S + Y + +P + Sbjct: 305 EQNTKTETTEATITMQKSDFHLTYPAGL-------------VSVEASSDVSYSV-DPFSE 350 Query: 412 SFRLPEAGGSVRVAEYTVASILPESDPAVLRASAGMDPVSVR----DSYLQLPAQLPRRV 467 S + Y V +PE L+A + Y QLP LP+RV Sbjct: 351 KIYTMHGDSSTTLHSYKVTYEIPEFPIENLKAVNTNEGQETNPYFMTKYTQLPESLPQRV 410 Query: 468 AELAGRLTASADTRYAAVSAVQDYL-QNGFTY-TLKTRIPPQGSDFTDDFLFVTKQGYCV 525 +LA LT D RY V A+++Y N F Y T+ P + D+ D FLF TK GYC Sbjct: 411 KDLAVNLTNDKDNRYDKVLAIENYFTDNSFVYETMNVSFPAKSQDYVDQFLFDTKSGYCN 470 Query: 526 HFATAMTVLLRSSGIPARYVQGYGPG----TLAADAVPPRYDVTQGDAHAWVEVYFPGTG 581 +F+T+M VLLRS+GIPAR+V+GY G TLA+ Y +T +AH+WVEVYFPG G Sbjct: 471 NFSTSMIVLLRSAGIPARWVKGYTEGTLDNTLASAEGQDIYKITNDNAHSWVEVYFPGYG 530 Query: 582 WVPFDPT 588 W+PF+PT Sbjct: 531 WIPFEPT 537 >B7HPA0.1 B7HPA0_BACC7 Putative membrane-bound protease Length = 635 Score = 165 bits (417), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 104/307 (33%), Positives = 148/307 (48%), Gaps = 27/307 (8%) Query: 295 EAELGAPLASSTEAVFSVVADRPSYWRGESIAYYDGRRW-IRDGAAFLPF--NLTALPGP 351 +++LG P + VF+ YWR E+ +Y G+ W + + + F N + Sbjct: 149 DSKLGGPFTADPTIVFTAQTQNKQYWRVETKDFYTGKGWEVSEKQKKVTFKNNNDVVSWY 208 Query: 352 GQNQAEEAGKSRRLVQRIQFAVPSSGGLPLFGAGTVADIENITLTDGSRLGYVLANPQKD 411 QN E K+ +Q+ + GL I++ S + Y + +P + Sbjct: 209 EQNTKTETTKATITMQKRYPHLTYPAGL-------------ISVEASSDISYSV-DPFSE 254 Query: 412 SFRLPEAGGSVRVAEYTVASILPESDPAVLRASAGMDPVSVR----DSYLQLPAQLPRRV 467 + S + Y V +PE L+A + Y QLP LPRRV Sbjct: 255 KIYTMDGDSSTTLHSYKVTYEVPEFSIENLKAVKTNEGQETNPYFMTKYTQLPKSLPRRV 314 Query: 468 AELAGRLTASADTRYAAVSAVQDYLQN-GFTY-TLKTRIPPQGSDFTDDFLFVTKQGYCV 525 +LA LT DTRY V A+++Y + F Y T P Q D+ D FLF TK GYC Sbjct: 315 RDLAVSLTKDKDTRYDKVLAIENYFTDYSFIYETTNVSFPAQNQDYVDQFLFDTKSGYCN 374 Query: 526 HFATAMTVLLRSSGIPARYVQGYGPGTLAADAVPPR----YDVTQGDAHAWVEVYFPGTG 581 +F+T+M VLLRS+GIPAR+V+GY GTL V Y + +AH+WVEVYFPG G Sbjct: 375 NFSTSMIVLLRSAGIPARWVKGYTEGTLDNTLVGAEGEDVYKIANDNAHSWVEVYFPGYG 434 Query: 582 WVPFDPT 588 W+PF+PT Sbjct: 435 WIPFEPT 441 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.137 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,344,265,385 Number of extensions: 132314151 Number of successful extensions: 274372 Number of sequences better than 1.0e-05: 605 Number of HSP's gapped: 273956 Number of HSP's successfully gapped: 606 Length of query: 603 Length of database: 3,060,696,203 Length adjustment: 142 Effective length of query: 461 Effective length of database: 1,722,912,073 Effective search space: 794262465653 Effective search space used: 794262465653 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)