BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0442.0020.p (420 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KAA4.1 B3KAA4_9BACI Threonine dehydratase 678 0.0 B1D9U1.1 B1D9U1_9BACL Threonine dehydratase 617 e-175 C0Z614.1 C0Z614_BREBN Threonine dehydratase biosynthetic EC=4.3.... 597 e-169 Q5KZ28.1 Q5KZ28_GEOKA Threonine dehydratase (Threonine ammonia-l... 597 e-168 C3J3G9.1 C3J3G9_9BACI Threonine dehydratase 595 e-168 >B3KAA4.1 B3KAA4_9BACI Threonine dehydratase Length = 422 Score = 678 bits (1749), Expect = 0.0, Method: Compositional matrix adjust. Identities = 336/414 (81%), Positives = 374/414 (90%) Query: 7 RIVGMEDIVRAHHVLREVIVRTPLQLDAVLSARYGCSVYLKREDLQIVRSFKIRGAYNMI 66 R V MEDIVRAHHVLREVIVRTPLQ DAVLSA+Y C VYLKREDLQ+VRSFKIRGAYNMI Sbjct: 9 RAVSMEDIVRAHHVLREVIVRTPLQRDAVLSAKYDCEVYLKREDLQVVRSFKIRGAYNMI 68 Query: 67 RSLSAEDRAKGIVCASAGNHAQGVAYSCKALGIKGKIYMPSTTPNQKIKQVRKFGGEFVE 126 RSLS E+R+ GIVCASAGNHAQGVA+SC AL IKGKIYMPSTTPNQK+KQV++FGG VE Sbjct: 69 RSLSEEERSNGIVCASAGNHAQGVAFSCNALNIKGKIYMPSTTPNQKVKQVKRFGGGSVE 128 Query: 127 VILKGDTFDDAYDEALQACIDHSMTLIHPFDEPRIIAGNGTIAMEVMESLDKPADFMFVT 186 VIL GDTFDDAY EA++AC ++ MT IHPFDE +IIAGNGTI MEVME LD PAD+MFVT Sbjct: 129 VILTGDTFDDAYAEAIKACDEYGMTFIHPFDEAKIIAGNGTIGMEVMEELDAPADYMFVT 188 Query: 187 IGGGGLAAGVATYVKTVSPSTKVIGVEPSGAASMSEALQRGEVVTLEEINKFVDGAAVKR 246 IGGGGLAAGVATYVKTVSP+T+VIGVEP GAASM E+ ++GEVVT+ EI+KFVDGAAVKR Sbjct: 189 IGGGGLAAGVATYVKTVSPTTQVIGVEPLGAASMIESKKQGEVVTMHEIDKFVDGAAVKR 248 Query: 247 VGGLTYDICSRRLDDVVKVPEGKACTTILELYNENAIVVEPAGSLPIAALDLYRDQIRGK 306 VG LTYDIC +DD+V VPEGKACTTILELYNENAIVVEPAGSL +AALD+ RD+IRGK Sbjct: 249 VGQLTYDICCELVDDIVTVPEGKACTTILELYNENAIVVEPAGSLAVAALDMNRDRIRGK 308 Query: 307 TVVCIVSGGNNDIDRMQEIKERSLIYEGLKHYFMVNFPQRAGALREFLADVLGPGDDITR 366 TVVCI+SGGNNDIDRMQEIKERSLIYEGLKHYFM+NFPQRAGALREFL +VLGP DDI R Sbjct: 309 TVVCIISGGNNDIDRMQEIKERSLIYEGLKHYFMINFPQRAGALREFLEEVLGPDDDIAR 368 Query: 367 FEYTKKNDKENGPALVGIELQHKEDYTPLIERMQQKGVDYLEINKDPNLFNMLI 420 FEYTKK++KE+GPALVGIEL EDY PL+ERMQ+KG+DY E+NK+ NLFN+LI Sbjct: 369 FEYTKKHNKEDGPALVGIELLRPEDYGPLVERMQRKGLDYTELNKNMNLFNLLI 422 >B1D9U1.1 B1D9U1_9BACL Threonine dehydratase Length = 422 Score = 617 bits (1590), Expect = e-175, Method: Compositional matrix adjust. Identities = 291/412 (70%), Positives = 344/412 (83%) Query: 9 VGMEDIVRAHHVLREVIVRTPLQLDAVLSARYGCSVYLKREDLQIVRSFKIRGAYNMIRS 68 +G+EDIV A L++VI RTPLQ + VLS RYGC+VYLKREDLQIVRSFKIRGAY+++RS Sbjct: 11 IGLEDIVHAQMHLKDVITRTPLQYNRVLSERYGCNVYLKREDLQIVRSFKIRGAYHLMRS 70 Query: 69 LSAEDRAKGIVCASAGNHAQGVAYSCKALGIKGKIYMPSTTPNQKIKQVRKFGGEFVEVI 128 L E A G+VCASAGNHAQGVAYSC+ALG++GKIYMPSTTP QK+ QV FGG+FVEVI Sbjct: 71 LPPERLAAGVVCASAGNHAQGVAYSCQALGVQGKIYMPSTTPRQKVNQVSFFGGDFVEVI 130 Query: 129 LKGDTFDDAYDEALQACIDHSMTLIHPFDEPRIIAGNGTIAMEVMESLDKPADFMFVTIG 188 L GDTFDDAY EA+ M IHPFD+P+IIAGNGT+ E+ME+ + D++ ++G Sbjct: 131 LTGDTFDDAYAEAIAESKRSGMAFIHPFDDPKIIAGNGTVGQELMEAAETVPDYVLASVG 190 Query: 189 GGGLAAGVATYVKTVSPSTKVIGVEPSGAASMSEALQRGEVVTLEEINKFVDGAAVKRVG 248 GGGL AG+A+YVKTVSP TKVIGVEP GA S+ +L GEV LE I+KFVDGAAVKR+G Sbjct: 191 GGGLVAGIASYVKTVSPQTKVIGVEPEGAPSLRVSLDAGEVTPLEHIDKFVDGAAVKRIG 250 Query: 249 GLTYDICSRRLDDVVKVPEGKACTTILELYNENAIVVEPAGSLPIAALDLYRDQIRGKTV 308 LT+ IC LDDV +PEGK CTT+L+LYNENAIV EPAG+LPIAALD RD+I GKTV Sbjct: 251 DLTFSICRELLDDVRTIPEGKICTTLLDLYNENAIVAEPAGALPIAALDALRDEIAGKTV 310 Query: 309 VCIVSGGNNDIDRMQEIKERSLIYEGLKHYFMVNFPQRAGALREFLADVLGPGDDITRFE 368 VC++SGGNND+DRMQEIKERSLIYEGLKHYFM++FPQRAGALREFL DVLGP DDITRFE Sbjct: 311 VCVISGGNNDVDRMQEIKERSLIYEGLKHYFMLSFPQRAGALREFLDDVLGPNDDITRFE 370 Query: 369 YTKKNDKENGPALVGIELQHKEDYTPLIERMQQKGVDYLEINKDPNLFNMLI 420 YTKK++K+NGPALVGIEL+ +EDY LI RMQ+KG ++E+NKDP LFN+LI Sbjct: 371 YTKKHNKDNGPALVGIELKQREDYAALIGRMQKKGFQFVELNKDPVLFNLLI 422 >C0Z614.1 C0Z614_BREBN Threonine dehydratase biosynthetic EC=4.3.1.19 Length = 415 Score = 597 bits (1539), Expect = e-169, Method: Compositional matrix adjust. Identities = 285/412 (69%), Positives = 351/412 (85%) Query: 9 VGMEDIVRAHHVLREVIVRTPLQLDAVLSARYGCSVYLKREDLQIVRSFKIRGAYNMIRS 68 V +EDIV A+H L++V+ +TPLQ + VLS RYGC+VYLKREDLQIVRSFKIRGAY+ +R+ Sbjct: 4 VRVEDIVVANHALKDVVEKTPLQKNKVLSERYGCNVYLKREDLQIVRSFKIRGAYHFMRN 63 Query: 69 LSAEDRAKGIVCASAGNHAQGVAYSCKALGIKGKIYMPSTTPNQKIKQVRKFGGEFVEVI 128 LS +R +G+VCASAGNHAQGVAYSC L IKG I+MP+TTP QKI QV+ FGG +VEV+ Sbjct: 64 LSTIERERGVVCASAGNHAQGVAYSCNHLQIKGTIFMPATTPRQKISQVKLFGGSYVEVV 123 Query: 129 LKGDTFDDAYDEALQACIDHSMTLIHPFDEPRIIAGNGTIAMEVMESLDKPADFMFVTIG 188 L GDTFDD++ EA++ CI T +HPFD+P ++AG GT+ +E+M +++PADF+F++IG Sbjct: 124 LIGDTFDDSFAEAMKYCIQEDRTFVHPFDDPLVVAGQGTVGLEIMNDMEEPADFVFMSIG 183 Query: 189 GGGLAAGVATYVKTVSPSTKVIGVEPSGAASMSEALQRGEVVTLEEINKFVDGAAVKRVG 248 GGGLAAGV TYVK +SP+T++IGVEP+GAASM AL++ +VVTL+EI+KFVDGAAVK+VG Sbjct: 184 GGGLAAGVGTYVKGISPNTQIIGVEPAGAASMQAALEQNDVVTLDEIDKFVDGAAVKQVG 243 Query: 249 GLTYDICSRRLDDVVKVPEGKACTTILELYNENAIVVEPAGSLPIAALDLYRDQIRGKTV 308 LT IC LDD+V VPEGK CTTILELYN NAIVVEPAG+L IAALD YRDQI GK V Sbjct: 244 QLTMSICQELLDDIVLVPEGKVCTTILELYNSNAIVVEPAGALSIAALDYYRDQIAGKNV 303 Query: 309 VCIVSGGNNDIDRMQEIKERSLIYEGLKHYFMVNFPQRAGALREFLADVLGPGDDITRFE 368 VC++SGGNNDIDRMQEIKERSL++EGLKHY+++NFPQRAGALREF+ VLGP DDITRFE Sbjct: 304 VCVISGGNNDIDRMQEIKERSLLHEGLKHYYVINFPQRAGALREFMEKVLGPHDDITRFE 363 Query: 369 YTKKNDKENGPALVGIELQHKEDYTPLIERMQQKGVDYLEINKDPNLFNMLI 420 YTKKN+KENGPALVGIE++ K+DY PL+ RM+Q G+ Y+EI DP LFN+LI Sbjct: 364 YTKKNNKENGPALVGIEMKCKDDYQPLVSRMKQHGIRYVEITNDPYLFNLLI 415 >Q5KZ28.1 Q5KZ28_GEOKA Threonine dehydratase (Threonine ammonia-lyase) EC=4.3.1.19 Length = 423 Score = 597 bits (1538), Expect = e-168, Method: Compositional matrix adjust. Identities = 286/412 (69%), Positives = 350/412 (84%) Query: 9 VGMEDIVRAHHVLREVIVRTPLQLDAVLSARYGCSVYLKREDLQIVRSFKIRGAYNMIRS 68 V +EDI+ A+H L++V+ TPLQ + +LS RY C+VYLKREDLQ+VRSFK+RGAYN ++ Sbjct: 12 VYVEDILIAYHTLKDVVFHTPLQKNPLLSERYECNVYLKREDLQVVRSFKLRGAYNRMKH 71 Query: 69 LSAEDRAKGIVCASAGNHAQGVAYSCKALGIKGKIYMPSTTPNQKIKQVRKFGGEFVEVI 128 L+ E+R G+VCASAGNHAQGVAYSC+ALG+ GKIYMP+TTP QK+ QV+ FG + VE++ Sbjct: 72 LTDEERRNGVVCASAGNHAQGVAYSCRALGVHGKIYMPATTPRQKVSQVQLFGKDMVEIV 131 Query: 129 LKGDTFDDAYDEALQACIDHSMTLIHPFDEPRIIAGNGTIAMEVMESLDKPADFMFVTIG 188 L GDTFDD+Y+EA++ T IHPFD+ +IAG GTI +EV+ D+P DF+F +IG Sbjct: 132 LVGDTFDDSYNEAVKCAEAEGRTFIHPFDDEYVIAGQGTIGVEVLNDCDEPIDFLFASIG 191 Query: 189 GGGLAAGVATYVKTVSPSTKVIGVEPSGAASMSEALQRGEVVTLEEINKFVDGAAVKRVG 248 GGGL AG+ TYVK++SPSTKVIGVEP+GA SM AL+RG VVTL+EI+KFVDGAAVK VG Sbjct: 192 GGGLMAGLGTYVKSISPSTKVIGVEPAGAPSMKAALERGHVVTLDEIDKFVDGAAVKTVG 251 Query: 249 GLTYDICSRRLDDVVKVPEGKACTTILELYNENAIVVEPAGSLPIAALDLYRDQIRGKTV 308 T+ +C LDD+V VPEGK CTTILELYNENAIV EPAG+LPIAALD Y++QIRGKTV Sbjct: 252 EKTFALCRGVLDDIVVVPEGKVCTTILELYNENAIVAEPAGALPIAALDFYKEQIRGKTV 311 Query: 309 VCIVSGGNNDIDRMQEIKERSLIYEGLKHYFMVNFPQRAGALREFLADVLGPGDDITRFE 368 VC++SGGNNDIDRMQEIKERS+IYEGL+HYF+VNFPQRAGALREFL +VLGP DDITRFE Sbjct: 312 VCVISGGNNDIDRMQEIKERSMIYEGLQHYFIVNFPQRAGALREFLDEVLGPTDDITRFE 371 Query: 369 YTKKNDKENGPALVGIELQHKEDYTPLIERMQQKGVDYLEINKDPNLFNMLI 420 YTKKN+KE+GPALVGIEL+ +EDY PLIERM++KG + E+NKDPNLF++LI Sbjct: 372 YTKKNNKESGPALVGIELKRREDYAPLIERMKKKGFPFQEVNKDPNLFHLLI 423 >C3J3G9.1 C3J3G9_9BACI Threonine dehydratase Length = 423 Score = 595 bits (1533), Expect = e-168, Method: Compositional matrix adjust. Identities = 285/412 (69%), Positives = 349/412 (84%) Query: 9 VGMEDIVRAHHVLREVIVRTPLQLDAVLSARYGCSVYLKREDLQIVRSFKIRGAYNMIRS 68 V +EDI+ A+H L++V+ TPLQ + +LS RY C+VYLKREDLQ+VRSFK+RGAYN ++ Sbjct: 12 VYVEDILIAYHTLKDVVFHTPLQKNPLLSERYECNVYLKREDLQVVRSFKLRGAYNRMKH 71 Query: 69 LSAEDRAKGIVCASAGNHAQGVAYSCKALGIKGKIYMPSTTPNQKIKQVRKFGGEFVEVI 128 L+ E+R G+VCASAGNHAQGVAYSC+ALG+ GKIYMP+TTP QK+ QV+ FG + VE++ Sbjct: 72 LTDEERRNGVVCASAGNHAQGVAYSCRALGVHGKIYMPATTPRQKVSQVQLFGKDMVEIV 131 Query: 129 LKGDTFDDAYDEALQACIDHSMTLIHPFDEPRIIAGNGTIAMEVMESLDKPADFMFVTIG 188 L GDTFDD+Y+EA++ T IHPFD+ +IAG GTI +EV+ D+P DF+F +IG Sbjct: 132 LVGDTFDDSYNEAVKCAEAEGRTFIHPFDDEYVIAGQGTIGVEVLNDCDEPIDFLFASIG 191 Query: 189 GGGLAAGVATYVKTVSPSTKVIGVEPSGAASMSEALQRGEVVTLEEINKFVDGAAVKRVG 248 GGGL AG+ TYVK++SPSTKVIGVEP+GA SM AL+ G VVTL+EI+KFVDGAAVK VG Sbjct: 192 GGGLMAGLGTYVKSISPSTKVIGVEPAGAPSMKAALEHGHVVTLDEIDKFVDGAAVKTVG 251 Query: 249 GLTYDICSRRLDDVVKVPEGKACTTILELYNENAIVVEPAGSLPIAALDLYRDQIRGKTV 308 T+ +C LDD+V VPEGK CTTILELYNENAIV EPAG+LPIAALD Y++QIRGKTV Sbjct: 252 EKTFALCRGVLDDIVVVPEGKVCTTILELYNENAIVAEPAGALPIAALDFYKEQIRGKTV 311 Query: 309 VCIVSGGNNDIDRMQEIKERSLIYEGLKHYFMVNFPQRAGALREFLADVLGPGDDITRFE 368 VC++SGGNNDIDRMQEIKERS+IYEGL+HYF+VNFPQRAGALREFL +VLGP DDITRFE Sbjct: 312 VCVISGGNNDIDRMQEIKERSMIYEGLQHYFIVNFPQRAGALREFLDEVLGPTDDITRFE 371 Query: 369 YTKKNDKENGPALVGIELQHKEDYTPLIERMQQKGVDYLEINKDPNLFNMLI 420 YTKKN+KE+GPALVGIEL+ +EDY PLIERM++KG + E+NKDPNLF++LI Sbjct: 372 YTKKNNKESGPALVGIELKRREDYAPLIERMKKKGFPFQEVNKDPNLFHLLI 423 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.138 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,544,105,647 Number of extensions: 110819970 Number of successful extensions: 312889 Number of sequences better than 1.0e-05: 3929 Number of HSP's gapped: 307970 Number of HSP's successfully gapped: 3932 Length of query: 420 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 282 Effective length of database: 1,760,596,133 Effective search space: 496488109506 Effective search space used: 496488109506 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)