BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0442.0017.p (181 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q187S7.1 Q187S7_CLOD6 Putative acyl-CoA thioesterase 238 2e-61 Q97DR5.1 Q97DR5_CLOAB Acyl-CoA thioesterase 1 97 6e-19 Q65LY2.1 Q65LY2_BACLD Putative uncharacterized protein 87 5e-16 C1US90.1 C1US90_9DELT Acyl-CoA thioester hydrolase/bile acid-CoA... 84 6e-15 C2LPY4.1 C2LPY4_STRSL Acyl-CoA thioesterase 1 82 3e-14 >Q187S7.1 Q187S7_CLOD6 Putative acyl-CoA thioesterase Length = 432 Score = 238 bits (606), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 114/173 (65%), Positives = 148/173 (85%) Query: 1 MELPWCSGDEFSSYAVFVADETGKVDFDLAKPVEGSYETANSMGLIYSLKQSTSTGKNIA 60 ME PWC G+EFSSY VF ++E G+VD DL +P+EG+Y+ N MGLIYSL++S + GKN A Sbjct: 35 MEFPWCKGEEFSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFA 94 Query: 61 ENISIEKPIIMHLLFESSIERKEVQLKRLFKAEDILVKQINESFTGQLFYQKNTNNKTVL 120 ENISI+KPIIM+++FE+SIERKEV+LKR+FK+EDI K I++ F G+LFY++N+NNKT+L Sbjct: 95 ENISIDKPIIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTIL 154 Query: 121 MVGGSDGQMQSLALMAGPLASRGFNVLITSYFASKGLPEKLEEVPLEYFEKIF 173 M+GGSDG + +L L+A PLASRGFNVL +YFA +GLP+KLEEVPLEYFEK+F Sbjct: 155 MLGGSDGNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVF 207 >Q97DR5.1 Q97DR5_CLOAB Acyl-CoA thioesterase 1 Length = 431 Score = 97.1 bits (240), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 57/174 (32%), Positives = 96/174 (55%), Gaps = 8/174 (4%) Query: 5 WCSGDEFSSYAVFVADETGKVDFDLAKPVEGSYETANSMGLIYSLKQSTSTGKNIAENIS 64 W F S+AVF AD+ G VD P++GSY +SMGL +S+ + + +++S Sbjct: 56 WVKKTNFESFAVFKADDKGTVDLSKDAPIKGSYTRVDSMGLFWSMVSTQQQDYTLKKSLS 115 Query: 65 IEKPII--MHLLFESSIERKEV---QLKRLFKAEDILVKQINES-FTGQLFYQKNTN-NK 117 P + M ++ + + KE+ + K+LF + D+ + + E+ G+ F KN+ K Sbjct: 116 -SCPFLTSMEVILKVEVCGKELVSSKCKKLFVSSDVKCQYVTENNLVGKYFTNKNSKIKK 174 Query: 118 TVLMVGGSDGQMQSLALMAGPLASRGFNVLITSYFASKGLPEKLEEVPLEYFEK 171 ++++ GS+G + + + +A LAS G+ L +YF LP L E+PLEYFEK Sbjct: 175 GIIVLSGSEGGIHNASQIAALLASHGYPSLALAYFGMDTLPATLNEIPLEYFEK 228 >Q65LY2.1 Q65LY2_BACLD Putative uncharacterized protein Length = 425 Score = 87.4 bits (215), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 55/169 (32%), Positives = 94/169 (55%), Gaps = 16/169 (9%) Query: 10 EFSSYAVFVADETGKVDFDLAKPVEGSYETANSMGLIYSLKQSTSTGKNIAENISIEKPI 69 E+ S+AVF A+ G+VD A P++G+Y++ + MGL +S++ S+ S K Sbjct: 46 EWRSHAVFQANRLGEVDPAAAAPLKGTYQSKDQMGLFWSMQAVESS-------CSFYKKS 98 Query: 70 IMHLLFESSIERKEV-----QLKRLFKAEDILVKQINESFTGQLFYQKNTNNK--TVLMV 122 + + +++KE ++KR+ +E + ++NE F++ N+ ++++ Sbjct: 99 VHPSAYRIEVKQKERIILSKEIKRVLLSETVDRLEVNEDGLCGTFFRPLAVNRPPAIIIL 158 Query: 123 GGSDGQMQSLALMAGPLASRGFNVLITSYFASKGLPEKLEEVPLEYFEK 171 GGSDG + MAG LA+ G+ L YF K LP+KL E+PLEYF+K Sbjct: 159 GGSDGGLDET--MAGMLANYGYATLAIPYFQYKQLPKKLVEIPLEYFQK 205 >C1US90.1 C1US90_9DELT Acyl-CoA thioester hydrolase/bile acid-CoA acetyltransferase Length = 471 Score = 84.0 bits (206), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 56/157 (35%), Positives = 83/157 (52%), Gaps = 12/157 (7%) Query: 23 GKVDFDLAKPVEGSYETANSMGLIYSLKQSTSTGKNIAENISIEKPIIMHLLFESSIERK 82 G VD P+EGSYE+A++MGLI+S+ ST + A +P+ + E + K Sbjct: 95 GTVDLGRDAPLEGSYESADAMGLIWSMTPSTEDWEQHARAAPQLQPMRIRYEAERTGADK 154 Query: 83 ---EVQLKRLFKAEDILVKQINE-SFTGQLFY----QKNTNNKTVLMVGGSDGQM-QSLA 133 V ++RL AED+ ++ G L+ VL++GGS+G + +S A Sbjct: 155 VFAAVDVERLRIAEDVRRSEVRAGGVVGNLYLPASASAQAPAPAVLVLGGSEGGLRESTA 214 Query: 134 LMAGPLASRGFNVLITSYFASKGLPEKLEEVPLEYFE 170 + LAS GF + +YFA GLP KLE +PLEYF+ Sbjct: 215 AL---LASHGFASFVVAYFAFDGLPPKLENIPLEYFD 248 >C2LPY4.1 C2LPY4_STRSL Acyl-CoA thioesterase 1 Length = 409 Score = 81.6 bits (200), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 52/164 (31%), Positives = 86/164 (52%), Gaps = 8/164 (4%) Query: 11 FSSYAVFVADETGKVDFDLAKPVEGSYETANSMGLIYSLKQSTSTGKNIAENISIEKPII 70 + S A FV+D+ G +D GSYE +MGL ++ K T+ K + ++S + P++ Sbjct: 55 WKSAATFVSDKNGIIDISQTPSCSGSYEDIATMGLFFNAKPLTNRKKKLPNSLS-KIPLL 113 Query: 71 MHLLFESSIER-----KEVQLKRLFKAEDILVKQI-NESFTGQLFYQKNT-NNKTVLMVG 123 H E I + E R + + I + I + F G+LFY K +++V Sbjct: 114 DHFFVEIKIIQGNTVVAERTFTRHYMSSQISHQDIYGKHFQGRLFYDKKAIKAPALIIVS 173 Query: 124 GSDGQMQSLALMAGPLASRGFNVLITSYFASKGLPEKLEEVPLE 167 GS+G+++ +A L+SRG+ L +YF +GLP+ LE +PLE Sbjct: 174 GSEGRIEKAQNIAQLLSSRGYICLAVAYFGLEGLPKHLERIPLE 217 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.133 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 980,426,303 Number of extensions: 36157683 Number of successful extensions: 105040 Number of sequences better than 1.0e-05: 41 Number of HSP's gapped: 105024 Number of HSP's successfully gapped: 41 Length of query: 181 Length of database: 3,060,696,203 Length adjustment: 127 Effective length of query: 54 Effective length of database: 1,864,227,298 Effective search space: 100668274092 Effective search space used: 100668274092 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)