BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0438.0003.p (321 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q2BBL0.1 Q2BBL0_9BACI Possible signal transduction protein (Cont... 261 5e-68 A3IDA0.1 A3IDA0_9BACI Signaling protein (EAL,CBS,GGDEF domains) 242 3e-62 C4IJX8.1 C4IJX8_CLOBU Diguanylate cyclase/phosphodiesterase 193 2e-47 B1QZI6.1 B1QZI6_CLOBU Possible signal transduction protein 193 2e-47 B8DJX8.1 B8DJX8_DESVM Diguanylate cyclase/phosphodiesterase 185 5e-45 >Q2BBL0.1 Q2BBL0_9BACI Possible signal transduction protein (Containing EAL, CBS and GGDEF domains) Length = 334 Score = 261 bits (668), Expect = 5e-68, Method: Compositional matrix adjust. Identities = 132/318 (41%), Positives = 192/318 (60%), Gaps = 4/318 (1%) Query: 1 MFLQIGEIAEQIPEISIHHKCAYVDHIFKSNPRLQGVAVTDNGQPVALIMRIRFYQQIGT 60 M IGEIAE++ ++ KC YV IFK P ++GV V + +PV L+ + +FYQ++ T Sbjct: 1 MQATIGEIAERVMFVAPSTKCEYVYSIFKETPEIEGVVVCMDSRPVGLVSKTKFYQKLST 60 Query: 61 LYGFTLYMGRSIELIMDGSPLVVEYKTPITEVSKLAMDRSEEHLYDYVLVTREGVMFGAV 120 YGF L+M R +EL+M PLV+++ PITE S LAMDR++E LYDYV+VT+E ++ G V Sbjct: 61 QYGFDLFMKRKVELVMIPEPLVIDHSVPITEASALAMDRTQEQLYDYVIVTKEDLLIGTV 120 Query: 121 SVRDLLLNFAEIQAVAASFLNPLTGLPGNLSINEWMVKSLLQEQFSVLYIDLDHFKAYND 180 S+R+LL+ AE Q A + NPLTGLPGN I + K++ +Q++VLYID++ FK +ND Sbjct: 121 SIRNLLMKLAEEQISIARYSNPLTGLPGNFEIKNALKKAMSSDQYTVLYIDINSFKTFND 180 Query: 181 TYGFKEGDRMLQATAEILKHC----TLRLEXXXXXXXXXXXXXXXXXYRYEEYCRTIIAE 236 T+GFK GD ++QATA I+K + + Y+ C I+ Sbjct: 181 TFGFKTGDEVIQATAAIMKDVIAASPMSSHSFIGHIGGDDFIAVFPHHNYQNICSAILER 240 Query: 237 FDRILQGFYHPGHLMQQHVLTESRSGVVEEIPLVSISIAVVTNRYRHFDSIEDLSGEAAR 296 FD + FY L + + +R G+ + +P++ +SIAVV N F +IE+LS EAAR Sbjct: 241 FDDYARSFYSEEELKEGCIQAVNRMGIPDSVPILGLSIAVVLNTNTDFQTIEELSREAAR 300 Query: 297 LKKKSKMIRGSSFVDDRD 314 LKK K R S ++ D Sbjct: 301 LKKHCKSFRKSVYLTLED 318 >A3IDA0.1 A3IDA0_9BACI Signaling protein (EAL,CBS,GGDEF domains) Length = 316 Score = 242 bits (618), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 126/308 (40%), Positives = 188/308 (61%), Gaps = 2/308 (0%) Query: 3 LQIGEIAEQIPEISIHHKCAYVDHIFKSNPRLQGVAVTDNGQPVALIMRIRFYQQIGTLY 62 ++IGE+ E++P I + K VD +F SNP L+ V V + +P+ I R FYQ+IGT Y Sbjct: 1 MKIGEVIEKVPCIDEYVKNKEVDVLFTSNPSLRSVVVVRDEKPIGHITRTHFYQKIGTQY 60 Query: 63 GFTLYMGRSIELIMDGSPLVVEYKTPITEVSKLAMDRSEEHLYDYVLVTREGVMFGAVSV 122 G+ L+MGR +LI+ +PL+V+ PITEVS AM R E LYD V+VT++ + +G VS+ Sbjct: 61 GYHLFMGRQNQLIIKENPLIVDRNIPITEVSTQAMSRPPEDLYDDVIVTKKDLYYGMVSI 120 Query: 123 RDLLLNFAEIQAVAASFLNPLTGLPGNLSINEWMVKSLLQEQFSVLYIDLDHFKAYNDTY 182 R+LLL + Q ASFLNPL+ LPGN INE + ++L Q+S++Y DLDHFK+YNDTY Sbjct: 121 RELLLKLVDTQVAIASFLNPLSSLPGNKLINEKLEEALKLPQYSLIYFDLDHFKSYNDTY 180 Query: 183 GFKEGDRMLQATAEILKHCTLRLEXXXXXXXXXXXXXXXXXYRYEEYCRTIIAEFDRILQ 242 GF +GD++L +ILK ++ Y C+ II +FD + Sbjct: 181 GFNKGDKILLYLTDILKRHICEVDDFLGHIGGDDFVAILPYYDAASICQRIIDDFDANIL 240 Query: 243 GFYHPGHLMQQHVLTESRSGVVEEIPLVSISIAVVTNRYRHFDSIEDLSGEAARLKKKSK 302 FY L ++V +R G +E + ++S+AV+TN++++F S+E LS +KK+ K Sbjct: 241 SFYELEDL--EYVQVANRIGKLEPLTCTTLSVAVITNKFQYFGSVEQLSNAVTHVKKQCK 298 Query: 303 MIRGSSFV 310 I GS ++ Sbjct: 299 KITGSCYL 306 >C4IJX8.1 C4IJX8_CLOBU Diguanylate cyclase/phosphodiesterase Length = 590 Score = 193 bits (490), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 109/307 (35%), Positives = 172/307 (56%), Gaps = 2/307 (0%) Query: 5 IGEIAEQIPEISIHHKCAYVDHIFKSNPRLQGVAVTDNGQPVALIMRIRFYQQIGTLYGF 64 I +IA+ IS + + VD +FK N + G+ V ++ I R Y ++G YG+ Sbjct: 281 IKDIAKSKIYISPNMPVSSVDLLFKKNKDIFGLCVVEDNVVKGTITRNALYSRLGWQYGY 340 Query: 65 TLYMGRSIELIMDGSPLVVEYKTPITEVSKLAMDRSEEHLYDYVLVTREGVMFGAVSVRD 124 ++Y + I IM+ L V+YK I V K AM R++E++YD+V VT++ +G V+++D Sbjct: 341 SIYYSKPISTIMETDFLSVDYKMSIERVIKFAMARAKENIYDHVTVTKDSKYYGIVTIKD 400 Query: 125 LLLNFAEIQAVAASFLNPLTGLPGNLSINEWMVKSLLQ-EQFSVLYIDLDHFKAYNDTYG 183 L+ EI+ A + NPLTGLPGN I + + K + E++ VLY D+D+FKAYND YG Sbjct: 401 LIEKTTEIEVNNARYSNPLTGLPGNFLIEKEIEKCISHYEEYCVLYFDIDNFKAYNDVYG 460 Query: 184 FKEGDRMLQATAEILKHCTLRLEXXXXXXXXXXXXXXXXXYRYEEYCRTIIAEFDRILQG 243 F+ GDR+++ A+I+ + C +II EFD + Sbjct: 461 FENGDRIIKLLADIINR-NISNRNFIGHIGGDDFISIIYSGDINNICESIIKEFDELKLV 519 Query: 244 FYHPGHLMQQHVLTESRSGVVEEIPLVSISIAVVTNRYRHFDSIEDLSGEAARLKKKSKM 303 +Y L + ++ ++R G+ E PLVSISIA +T + FDS+ L+ EA+++KKK K Sbjct: 520 YYDEKDLKRGYIEAQNRHGIEERFPLVSISIAGITKKEVTFDSVYLLAKEASQIKKKCKQ 579 Query: 304 IRGSSFV 310 I GS ++ Sbjct: 580 INGSCYI 586 >B1QZI6.1 B1QZI6_CLOBU Possible signal transduction protein Length = 590 Score = 193 bits (490), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 109/307 (35%), Positives = 172/307 (56%), Gaps = 2/307 (0%) Query: 5 IGEIAEQIPEISIHHKCAYVDHIFKSNPRLQGVAVTDNGQPVALIMRIRFYQQIGTLYGF 64 I +IA+ IS + + VD +FK N + G+ V ++ I R Y ++G YG+ Sbjct: 281 IKDIAKSKIYISPNMPVSSVDLLFKKNKDIFGLCVVEDNVVKGTITRNALYSRLGWQYGY 340 Query: 65 TLYMGRSIELIMDGSPLVVEYKTPITEVSKLAMDRSEEHLYDYVLVTREGVMFGAVSVRD 124 ++Y + I IM+ L V+YK I V K AM R++E++YD+V VT++ +G V+++D Sbjct: 341 SIYYSKPISTIMETDFLSVDYKMSIERVIKFAMARAKENIYDHVTVTKDSKYYGIVTIKD 400 Query: 125 LLLNFAEIQAVAASFLNPLTGLPGNLSINEWMVKSLLQ-EQFSVLYIDLDHFKAYNDTYG 183 L+ EI+ A + NPLTGLPGN I + + K + E++ VLY D+D+FKAYND YG Sbjct: 401 LIEKTTEIEVNNARYSNPLTGLPGNFLIEKEIEKCISHYEEYCVLYFDIDNFKAYNDVYG 460 Query: 184 FKEGDRMLQATAEILKHCTLRLEXXXXXXXXXXXXXXXXXYRYEEYCRTIIAEFDRILQG 243 F+ GDR+++ A+I+ + C +II EFD + Sbjct: 461 FENGDRIIKLLADIINR-NISNRNFIGHIGGDDFISIIYSGDINNICESIIKEFDELKLV 519 Query: 244 FYHPGHLMQQHVLTESRSGVVEEIPLVSISIAVVTNRYRHFDSIEDLSGEAARLKKKSKM 303 +Y L + ++ ++R G+ E PLVSISIA +T + FDS+ L+ EA+++KKK K Sbjct: 520 YYDEKDLKRGYIEAQNRHGIEERFPLVSISIAGITKKEVTFDSVYLLAKEASQIKKKCKQ 579 Query: 304 IRGSSFV 310 I GS ++ Sbjct: 580 INGSCYI 586 >B8DJX8.1 B8DJX8_DESVM Diguanylate cyclase/phosphodiesterase Length = 883 Score = 185 bits (469), Expect = 5e-45, Method: Compositional matrix adjust. Identities = 109/313 (34%), Positives = 168/313 (53%), Gaps = 7/313 (2%) Query: 3 LQIGEIAEQIPEISIHHKCAYVDHIFKSNPRLQGVAVTD-NGQPVALIMRIRFYQQIGTL 61 + +GE+AE + V +F+++ L +AVTD G+P+ LIM +Q+ Sbjct: 561 MPVGELAEPAHTVPPTTLAGEVRRLFETSDPLTSIAVTDERGKPLGLIMDYHLNRQLSAQ 620 Query: 62 YGFTLYMGRSIELIMDGSPLVVEYKTPITEVSKLAMDRSEEHLYDYVLVTREGVMFGAVS 121 YG LY R + +MD PLV+E TP+ + ++LAM RS YD +++TR GV+ G VS Sbjct: 621 YGVALYFKRPVSAVMDARPLVLEQTTPVEDAARLAMSRSRLQAYDDIMITRAGVLAGTVS 680 Query: 122 VRDLLLNFAEIQAVAASFLNPLTGLPGNLSINEWMVKSLLQEQ-FSVLYIDLDHFKAYND 180 V+ LL AE+Q A NPLTGLPGN+++ + + L Q FS+LY DLD+FK+YND Sbjct: 681 VQRLLHKLAEVQVELAKGTNPLTGLPGNVALEKELETRLAGGQPFSLLYADLDNFKSYND 740 Query: 181 TYGFKEGDRMLQATAEILKHCTLR--LEXXXXXXXXXXXXXXXXXYRY-EEYCRTIIAEF 237 TYGFK GDR++ A++L R + R+ E CR F Sbjct: 741 TYGFKNGDRIILLLAKVLSWAVQRHGAKGDYVAHIGGDDFVCITSPRHAERVCRAATRCF 800 Query: 238 DRILQGFYHPGHLMQQHVLTESRSGVVEEIPLVSISIAVVTNRYRHFDSIEDLSGEAARL 297 R+++G Y + + + R G+ P VS+S+AV+ R ++ ++ AA + Sbjct: 801 GRLVRGCYCAEDIARGWITARGRDGMERRFPFVSVSLAVLDCHER--SNLHEIGERAAHV 858 Query: 298 KKKSKMIRGSSFV 310 K +K I G+++V Sbjct: 859 KHLAKAIPGNAYV 871 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.138 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,711,735,928 Number of extensions: 63641170 Number of successful extensions: 153607 Number of sequences better than 1.0e-05: 271 Number of HSP's gapped: 155040 Number of HSP's successfully gapped: 278 Length of query: 321 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 186 Effective length of database: 1,788,859,178 Effective search space: 332727807108 Effective search space used: 332727807108 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)