BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0437.0019.p (343 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A9KHK3.1 A9KHK3_CLOPH Glycosyl hydrolase family 88 558 e-157 A7B573.1 A7B573_RUMGN Putative uncharacterized protein 446 e-123 B1D8F9.1 B1D8F9_9BACL Glycosyl hydrolase family 88 443 e-122 B3KBX3.1 B3KBX3_9BACI Glycosyl hydrolase family 88 435 e-120 B1DB32.1 B1DB32_9BACL Glycosyl hydrolase family 88 397 e-108 >A9KHK3.1 A9KHK3_CLOPH Glycosyl hydrolase family 88 Length = 345 Score = 558 bits (1439), Expect = e-157, Method: Compositional matrix adjust. Identities = 252/338 (74%), Positives = 298/338 (88%) Query: 1 MLQLKVDREEILSIIDRVTRKTLAMDLTWEWPCGVAYYGVSRAYQTTGNKEYLDRLVQWT 60 ML+++ DRE IL +IDRV +KT+ MD+TW+WPCGVAYYG+S AY+ T N+EY++ L QWT Sbjct: 1 MLKIEYDRENILEVIDRVVKKTMTMDMTWDWPCGVAYYGISEAYRVTKNEEYINLLKQWT 60 Query: 61 DEYIGLGLPDWTVNTCAMGHMLITLYEETGNQKYWDIVMSKVDYIRNDALRFGDKVLQHT 120 DEYI LGLP+WTVNTCAMGH +ITLYE T ++KYWDIVMSK+DYIRN ALRFGD VLQHT Sbjct: 61 DEYIALGLPNWTVNTCAMGHCMITLYEATNDEKYWDIVMSKIDYIRNKALRFGDNVLQHT 120 Query: 121 VSVSNDFPEQAWADTLFMAAFFLLRAGSKINDQDLIQDALNQYYWHIKYLQDPGSGFWFH 180 VSV+NDFPEQAWADTLFMAAFFLLR G K+ D D+I DALNQYYWHI+YLQ+P + W+H Sbjct: 121 VSVNNDFPEQAWADTLFMAAFFLLRVGVKLKDPDMIDDALNQYYWHIQYLQEPRTSLWYH 180 Query: 181 GYNHVQQDHMSGLFWGRANAWGAYTMSQVKPLLKDWYLYPQCMDVECSLRDQLAALKLVQ 240 GYN++ +DHMSG +WGRANAW AYTMSQV L + YLYP M+++C+LRDQLAALKL+Q Sbjct: 181 GYNNINKDHMSGFYWGRANAWAAYTMSQVSKRLPEPYLYPNYMEIDCALRDQLAALKLLQ 240 Query: 241 TENGLWRTVLDDEESYEEVSASCGIAAAMINNGNPLHTKYVQKALQGILQNISEDGRVLG 300 T++GLWRT+LDDEESYEEVSASCGIAAAM+ N NPLHTKY+QKAL GIL+NISEDGRVL Sbjct: 241 TKDGLWRTILDDEESYEEVSASCGIAAAMVTNQNPLHTKYIQKALDGILKNISEDGRVLN 300 Query: 301 VSGGTAVMKDREGYRNIPKDWIQGWGQGLALAFLSDML 338 VSGGTAVMKDREGYRN+PK W+QGWGQGLAL+FLS ++ Sbjct: 301 VSGGTAVMKDREGYRNVPKTWMQGWGQGLALSFLSALV 338 >A7B573.1 A7B573_RUMGN Putative uncharacterized protein Length = 349 Score = 446 bits (1146), Expect = e-123, Method: Compositional matrix adjust. Identities = 215/342 (62%), Positives = 267/342 (78%) Query: 1 MLQLKVDREEILSIIDRVTRKTLAMDLTWEWPCGVAYYGVSRAYQTTGNKEYLDRLVQWT 60 M +L+ ++ + +D++ +T+ MD+TW+WPCGVAYYG++ AY+ T +EYL+ L + Sbjct: 1 MGKLEYNKANLEEQMDKIVARTMRMDMTWDWPCGVAYYGIAEAYEATKKQEYLNVLKERV 60 Query: 61 DEYIGLGLPDWTVNTCAMGHMLITLYEETGNQKYWDIVMSKVDYIRNDALRFGDKVLQHT 120 DE I LGLP WTVNTCAMGH LITLY+ TG +KY D++ K+ Y+RNDALRFG+ VLQHT Sbjct: 61 DELIELGLPVWTVNTCAMGHCLITLYQATGEEKYMDLIQFKIAYLRNDALRFGNHVLQHT 120 Query: 121 VSVSNDFPEQAWADTLFMAAFFLLRAGSKINDQDLIQDALNQYYWHIKYLQDPGSGFWFH 180 VS NDFPEQ WADTLFMAAFF+LR G D +LI DALNQ+ WHI+YLQD +G W+H Sbjct: 121 VSAKNDFPEQCWADTLFMAAFFMLRVGVMTEDAELIDDALNQFCWHIQYLQDEENGLWYH 180 Query: 181 GYNHVQQDHMSGLFWGRANAWGAYTMSQVKPLLKDWYLYPQCMDVECSLRDQLAALKLVQ 240 GYN++ +DHMSG WGRAN WGAYTM QV +L + YLYPQ +++ SL +QL+ALKL+Q Sbjct: 181 GYNNITKDHMSGFHWGRANCWGAYTMCQVGRILPECYLYPQYLEIVGSLNEQLSALKLLQ 240 Query: 241 TENGLWRTVLDDEESYEEVSASCGIAAAMINNGNPLHTKYVQKALQGILQNISEDGRVLG 300 TENGLWRT+LDDEESYEE+SAS GIAAA+ GNPLH KYVQK++ GIL +++ DGRV Sbjct: 241 TENGLWRTILDDEESYEEISASAGIAAALTLKGNPLHLKYVQKSIDGILAHVNPDGRVTD 300 Query: 301 VSGGTAVMKDREGYRNIPKDWIQGWGQGLALAFLSDMLKQRN 342 VSGGTAVMKDREGY I K WIQGWGQG+ALAF S +L N Sbjct: 301 VSGGTAVMKDREGYCGISKKWIQGWGQGMALAFFSAVLNVEN 342 >B1D8F9.1 B1D8F9_9BACL Glycosyl hydrolase family 88 Length = 345 Score = 443 bits (1139), Expect = e-122, Method: Compositional matrix adjust. Identities = 214/342 (62%), Positives = 260/342 (76%) Query: 2 LQLKVDREEILSIIDRVTRKTLAMDLTWEWPCGVAYYGVSRAYQTTGNKEYLDRLVQWTD 61 ++L ++ I+ +V T MDLTW+WP GVA+YGVSRAY+ TG K YL+R+ W D Sbjct: 1 MKLDATAIDLKQIVGQVADYTFGMDLTWDWPGGVAFYGVSRAYEVTGEKAYLERMASWVD 60 Query: 62 EYIGLGLPDWTVNTCAMGHMLITLYEETGNQKYWDIVMSKVDYIRNDALRFGDKVLQHTV 121 EYI +GLP WTVNTCAMGHML+TLYE T ++KY DI+MSKVDY+ N ALRFGD+VLQHTV Sbjct: 61 EYIEVGLPGWTVNTCAMGHMLLTLYEHTQDRKYLDIIMSKVDYLENKALRFGDRVLQHTV 120 Query: 122 SVSNDFPEQAWADTLFMAAFFLLRAGSKINDQDLIQDALNQYYWHIKYLQDPGSGFWFHG 181 S NDFPEQ WADTLFMAA+FLLR G +DLI+DAL+Q+YWHI YLQD G W+HG Sbjct: 121 SSKNDFPEQCWADTLFMAAYFLLRVGVMQGRKDLIEDALHQFYWHINYLQDEEDGLWYHG 180 Query: 182 YNHVQQDHMSGLFWGRANAWGAYTMSQVKPLLKDWYLYPQCMDVECSLRDQLAALKLVQT 241 YNH+ +DHMSG++W RANAW AYTMS+ + + YLYP M + SLRDQLAA+K +Q Sbjct: 181 YNHINKDHMSGMYWARANAWAAYTMSRAARGVPEGYLYPPMMHIWGSLRDQLAAIKRLQK 240 Query: 242 ENGLWRTVLDDEESYEEVSASCGIAAAMINNGNPLHTKYVQKALQGILQNISEDGRVLGV 301 E+GLW TVLD E+Y EVSA+ GIAAAM+ NPLH KYVQKAL G+L NI+ GRVL V Sbjct: 241 EDGLWGTVLDYPEAYGEVSATAGIAAAMVTQNNPLHGKYVQKALDGVLANITSSGRVLNV 300 Query: 302 SGGTAVMKDREGYRNIPKDWIQGWGQGLALAFLSDMLKQRNE 343 SGGTAVM D +GY +I K W QGWGQGLALA L+ +++ N Sbjct: 301 SGGTAVMNDIDGYLSIGKKWAQGWGQGLALALLAAVIENENS 342 >B3KBX3.1 B3KBX3_9BACI Glycosyl hydrolase family 88 Length = 347 Score = 435 bits (1118), Expect = e-120, Method: Compositional matrix adjust. Identities = 208/339 (61%), Positives = 257/339 (75%), Gaps = 1/339 (0%) Query: 2 LQLKVDREEILSIIDRVTRKTLAMDLTWEWPCGVAYYGVSRAYQTTGNKEYLDRLVQWTD 61 L+L++ E++ +RV T MDLTW+WP GVA+YGVSR TG YL R++ W + Sbjct: 3 LKLELTSEDLKQTAERVADYTFGMDLTWDWPGGVAFYGVSRLLDVTGEHVYLKRMIDWVE 62 Query: 62 EYIGLGLPD-WTVNTCAMGHMLITLYEETGNQKYWDIVMSKVDYIRNDALRFGDKVLQHT 120 EY+ GLP+ WTVNTCAMGHML+TLYEET +QKY DI++SK+DY+ + ALRFGD+VLQHT Sbjct: 63 EYLEAGLPEGWTVNTCAMGHMLLTLYEETKDQKYLDIILSKIDYLEHHALRFGDRVLQHT 122 Query: 121 VSVSNDFPEQAWADTLFMAAFFLLRAGSKINDQDLIQDALNQYYWHIKYLQDPGSGFWFH 180 VS +NDFPEQ WADTLFMAA+ LR G + + LI DAL+Q+ HI YLQD SG W+H Sbjct: 123 VSANNDFPEQCWADTLFMAAYLELRVGVLLEKKALIDDALHQFERHIHYLQDEDSGLWYH 182 Query: 181 GYNHVQQDHMSGLFWGRANAWGAYTMSQVKPLLKDWYLYPQCMDVECSLRDQLAALKLVQ 240 GYNHV+QDHMSG+ W RANAW AYTMS+ L + YLYP M + SLRDQLAA+K +Q Sbjct: 183 GYNHVKQDHMSGIHWARANAWAAYTMSRAARGLPEGYLYPPMMHIWSSLRDQLAAIKRLQ 242 Query: 241 TENGLWRTVLDDEESYEEVSASCGIAAAMINNGNPLHTKYVQKALQGILQNISEDGRVLG 300 E+GLW TVLD E+Y EVSA+ GIAAAM+ GNPLH KYV+KAL G+L NI+++GRVL Sbjct: 243 HEDGLWGTVLDYPEAYGEVSATAGIAAAMVMQGNPLHAKYVRKALDGVLANIADNGRVLN 302 Query: 301 VSGGTAVMKDREGYRNIPKDWIQGWGQGLALAFLSDMLK 339 VSGGTAVM D EGY I + W QGWGQGL LAFL+ +++ Sbjct: 303 VSGGTAVMNDIEGYLGIDRKWAQGWGQGLTLAFLAAVIE 341 >B1DB32.1 B1DB32_9BACL Glycosyl hydrolase family 88 Length = 345 Score = 397 bits (1019), Expect = e-108, Method: Compositional matrix adjust. Identities = 183/339 (53%), Positives = 251/339 (74%), Gaps = 2/339 (0%) Query: 1 MLQLKVDREEILSIIDRVTRKTLAMDLTWEWPCGVAYYGVSRAYQTTGNKEYLDRLVQWT 60 M + +V++EE+L +ID V R+T+ MD TW W CGVA+YG+S+A++ T N+EY+D LV+W Sbjct: 1 MKKFEVNQEEVLELIDLVVRRTMNMDFTWNWSCGVAFYGISKAWEVTKNQEYIDFLVKWV 60 Query: 61 DEYIGLGLPDWTVNTCAMGHMLITLYEETGNQKYWDIVMSKVDYIRNDALRFGDKVLQHT 120 DEY+ LGLP VN+CAMGH ++TL+E TG+QKY D+ + K +Y+R DA+RFG+ V QHT Sbjct: 61 DEYLELGLPALMVNSCAMGHTMLTLHEATGDQKYLDLALMKAEYLRKDAIRFGEGVFQHT 120 Query: 121 VSVSNDFPEQAWADTLFMAAFFLLRAGSKINDQDLIQDALNQYYWHIKYLQDPGSGFWFH 180 VS NDFPEQAWADTLFMAA+FLLR G K++ ++ I DALNQ+YWH +YLQD + ++H Sbjct: 121 VSSKNDFPEQAWADTLFMAAYFLLRLGFKLDKKEYIDDALNQFYWHEEYLQDTKTNLFYH 180 Query: 181 GYNHVQQDHMSGLFWGRANAWGAYTMSQVKPLLKDWYLYPQCMDVECSLRDQLAALKLVQ 240 +++V+ DH+SG++WGRANAW AYTM+Q +L P M + +L DQL++L +Q Sbjct: 181 AWDNVRGDHLSGIYWGRANAWAAYTMAQAHKMLNP--FMPMWMQLGGALGDQLSSLVRLQ 238 Query: 241 TENGLWRTVLDDEESYEEVSASCGIAAAMINNGNPLHTKYVQKALQGILQNISEDGRVLG 300 + +GLWRT+L+DE SYEE SAS GIAAA+ +PLH Y+ KA +GI NI +DG V Sbjct: 239 SPDGLWRTILNDETSYEETSASAGIAAALTIYAHPLHQNYMAKAYEGIRANIDDDGSVRN 298 Query: 301 VSGGTAVMKDREGYRNIPKDWIQGWGQGLALAFLSDMLK 339 VS GTAVM + Y+ I K +QGWGQGL LA+L +++ Sbjct: 299 VSAGTAVMYSADDYKVISKKRVQGWGQGLTLAYLVALVQ 337 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,179,549,719 Number of extensions: 93509318 Number of successful extensions: 204750 Number of sequences better than 1.0e-05: 241 Number of HSP's gapped: 204275 Number of HSP's successfully gapped: 241 Length of query: 343 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 207 Effective length of database: 1,779,438,163 Effective search space: 368343699741 Effective search space used: 368343699741 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)