BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0435.0009.p (400 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KEK9.1 B3KEK9_9BACI Drug resistance transporter, Bcr/CflA subf... 330 1e-88 Q2BFP4.1 Q2BFP4_9BACI Putative uncharacterized protein 317 1e-84 B1DBZ1.1 B1DBZ1_9BACL Drug resistance transporter, Bcr/CflA subf... 310 1e-82 P96709.1 YDGK_BACSU Uncharacterized MFS-type transporter ydgK 303 1e-80 A7Z1W6.1 A7Z1W6_BACA2 YdgK 301 8e-80 >B3KEK9.1 B3KEK9_9BACI Drug resistance transporter, Bcr/CflA subfamily Length = 330 Score = 330 bits (846), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 161/265 (60%), Positives = 199/265 (75%) Query: 23 GPFTVDMYLSSLPQLMSYFGTNTSMIXXXXXXXXXXXXXXXXXXXPISDVHGRRKPLLIS 82 GPFTVDMYL+SLPQ+ +FGT+ + + P+SD++GRR PLL S Sbjct: 23 GPFTVDMYLASLPQIAVFFGTSAAAVQASLTTSLLGLGLGQLIMGPLSDMYGRRPPLLTS 82 Query: 83 MILYLLTSLACAFSPNIGLFIALRFIQGFVASAGLVISRAIVRDRYNGIEMTKFMSLLTM 142 MILY+L+S+ CAF+P+I FI LRFIQG ASAGLVISRAIVRDR+ G+EMTKF++LLTM Sbjct: 83 MILYILSSIGCAFAPSIEWFICLRFIQGVAASAGLVISRAIVRDRFRGVEMTKFIALLTM 142 Query: 143 ISNIAPLISPTAGSTVTTFTSWXXXXXXXXXXXXXXXXXXXWGIKESLPAERRISSNFRE 202 ISN+APLISPT GS V +++SW G+KESLP ++R+ S+F Sbjct: 143 ISNVAPLISPTLGSAVMSYSSWGSVFLFLGALGIALTAITLGGLKESLPVQQRVPSSFST 202 Query: 203 VLRNYDSLLRDRSFMGYALVNGILFSGVFAYVAGTPFIYQNMYGISPQLFSILFALNGLG 262 +++NY+SL R+RSFMGYALVNGILF+GVFAYVAGTPFIYQNMYG+SPQ+FSILFALNGL Sbjct: 203 LIKNYNSLFRERSFMGYALVNGILFAGVFAYVAGTPFIYQNMYGVSPQMFSILFALNGLA 262 Query: 263 IILGSQFVKRLAGRMDERRIFQIGL 287 II GSQFVK AGR+ E R+F+IGL Sbjct: 263 IIAGSQFVKLFAGRVPESRLFRIGL 287 >Q2BFP4.1 Q2BFP4_9BACI Putative uncharacterized protein Length = 409 Score = 317 bits (812), Expect = 1e-84, Method: Compositional matrix adjust. Identities = 170/375 (45%), Positives = 242/375 (64%) Query: 23 GPFTVDMYLSSLPQLMSYFGTNTSMIXXXXXXXXXXXXXXXXXXXPISDVHGRRKPLLIS 82 GPFT+D YL S P +++ F TN S++ P+SDVHGRRKPLLI Sbjct: 22 GPFTIDTYLPSFPTIVNDFHTNASLVQISLTTCLLGLGLGQLVIGPMSDVHGRRKPLLIF 81 Query: 83 MILYLLTSLACAFSPNIGLFIALRFIQGFVASAGLVISRAIVRDRYNGIEMTKFMSLLTM 142 + LY L+S+ CA +PNI I RF+QGF A+ GLVISRAIVRD ++G E+TKF + L + Sbjct: 82 LTLYFLSSVTCAIAPNIYTLIVSRFLQGFAAAGGLVISRAIVRDLFSGRELTKFFASLML 141 Query: 143 ISNIAPLISPTAGSTVTTFTSWXXXXXXXXXXXXXXXXXXXWGIKESLPAERRISSNFRE 202 + N+ P+I+P G + TFTSW ++ESLPAE+R+ NF++ Sbjct: 142 VGNLGPIIAPIIGGAILTFTSWKGVFIVLAGIGIIFTFIVSLKLEESLPAEKRVPGNFKQ 201 Query: 203 VLRNYDSLLRDRSFMGYALVNGILFSGVFAYVAGTPFIYQNMYGISPQLFSILFALNGLG 262 V+ N+ LL+DR F GYA G + +G+FAYV+G F+YQN+YG+SPQ+FS LF +NG+G Sbjct: 202 VIGNFGMLLKDREFAGYAFTQGFITAGIFAYVSGISFVYQNIYGVSPQVFSFLFGVNGVG 261 Query: 263 IILGSQFVKRLAGRMDERRIFQIGLQLAFITTAAILMIVLAHGPFFALFISVFLFAASIG 322 +I+G+Q V RLA + E+ +IGL L+ +L +L P + + +F SI Sbjct: 262 LIIGTQLVGRLADYIPEKTFLKIGLGLSNTAAILLLTALLLKAPLLLVALPIFFLVMSIS 321 Query: 323 IIGPVSFTLAMESQGHIAGSAAAILGTLQFALGAVTSPLVGLGGDNSAIPFGITIFMTSI 382 IIG SF+LAME++GH+AGSA+A+LG L F LG++T+PLVG+ G+ SA+P GI I S+ Sbjct: 322 IIGTASFSLAMETKGHVAGSASALLGVLPFLLGSITAPLVGVAGEYSALPMGIIILAASL 381 Query: 383 LAVIAFVFLVKKPTL 397 LA +++ LV+K +L Sbjct: 382 LAFLSYFLLVRKGSL 396 >B1DBZ1.1 B1DBZ1_9BACL Drug resistance transporter, Bcr/CflA subfamily Length = 405 Score = 310 bits (795), Expect = 1e-82, Method: Compositional matrix adjust. Identities = 156/371 (42%), Positives = 227/371 (61%) Query: 24 PFTVDMYLSSLPQLMSYFGTNTSMIXXXXXXXXXXXXXXXXXXXPISDVHGRRKPLLISM 83 P ++DMYL +LP+L FG TSM PISDV GRRKPLLI + Sbjct: 31 PLSIDMYLPALPELADDFGAGTSMSQLSLTACLLGISLGQLFIGPISDVRGRRKPLLIGL 90 Query: 84 ILYLLTSLACAFSPNIGLFIALRFIQGFVASAGLVISRAIVRDRYNGIEMTKFMSLLTMI 143 I+Y+ S+ C +P+I F+ LRF+QGF +AG+VISRA+VRD + G MT+F S+L ++ Sbjct: 91 IVYMAASILCVAAPSIETFVLLRFVQGFGGAAGIVISRAVVRDLFEGAGMTRFFSMLMLV 150 Query: 144 SNIAPLISPTAGSTVTTFTSWXXXXXXXXXXXXXXXXXXXWGIKESLPAERRISSNFREV 203 + +AP+ +P AG + +T W +G+KESL + R R + Sbjct: 151 NGVAPIAAPIAGGQILKWTEWRGVFIVLTVIGAVMFAAVWFGMKESLQEKNRSQGGARNI 210 Query: 204 LRNYDSLLRDRSFMGYALVNGILFSGVFAYVAGTPFIYQNMYGISPQLFSILFALNGLGI 263 L + LL DR FMGYAL G + + +FAY++G+PF+ QN++G+SPQ +S+ FA+NGLGI Sbjct: 211 LTKFRGLLTDRVFMGYALTQGFVVAAMFAYISGSPFVIQNLFGVSPQGYSLCFAINGLGI 270 Query: 264 ILGSQFVKRLAGRMDERRIFQIGLQLAFITTAAILMIVLAHGPFFALFISVFLFAASIGI 323 I SQ RLAG++ ++ GL LAF A+L+ ++A FA+ + +F AS+GI Sbjct: 271 IAASQIAGRLAGKVSAPKLLVAGLSLAFAGGLALLVSIIAGWGLFAVLVPLFFVVASVGI 330 Query: 324 IGPVSFTLAMESQGHIAGSAAAILGTLQFALGAVTSPLVGLGGDNSAIPFGITIFMTSIL 383 + SF+LAM++Q AGSA+A+LG L F GA +PLVGLGG +A+P G+ I Sbjct: 331 VQTASFSLAMQNQQQAAGSASALLGLLSFVFGACMAPLVGLGGSEAALPMGMVIAAAETA 390 Query: 384 AVIAFVFLVKK 394 AV+++V LV++ Sbjct: 391 AVLSYVLLVRR 401 >P96709.1 YDGK_BACSU Uncharacterized MFS-type transporter ydgK Length = 402 Score = 303 bits (777), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 163/374 (43%), Positives = 233/374 (62%), Gaps = 3/374 (0%) Query: 23 GPFTVDMYLSSLPQLMSYFGTNTSMIXXXXXXXXXXXXXXXXXXXPISDVHGRRKPLLIS 82 GP +DMYL S P++ + S++ P+SD GRRKPLLI Sbjct: 24 GPLNIDMYLPSFPEIAKDLSASASLVQLSLTACLVGLTIGQLIVGPVSDAQGRRKPLLIC 83 Query: 83 MILYLLTSLACAFSPNIGLFIALRFIQGFVASAGLVISRAIVRDRYNGIEMTKFMSLLTM 142 + L+ L+SL CA SPNI +A RF+QGF ASAGLV+SRAIVRD + G E++KF SLL + Sbjct: 84 IFLFALSSLFCALSPNITTLVAARFLQGFTASAGLVLSRAIVRDVFTGRELSKFFSLLMV 143 Query: 143 ISNIAPLISPTAGSTVT--TFTSWXXXXXXXXXXXXXXXXXXXWGIKESLPAERRISSNF 200 I+ +AP+++P G + F +W +KE+LP E+RI S+ Sbjct: 144 ITAVAPMVAPMTGGAILLLPFATWHTIFHVLMIIGFLLVLLIALRLKETLPLEKRIPSSI 203 Query: 201 REVLRNYDSLLRDRSFMGYALVNGILFSGVFAYVAGTPFIYQNMYGISPQLFSILFALNG 260 ++ SLL+DRSFMGYAL G + G FAYV+GTPF+YQ++YG+SPQ+FSILF +NG Sbjct: 204 GTSVKTMGSLLKDRSFMGYALTVGFIHGGSFAYVSGTPFVYQDIYGVSPQVFSILFGING 263 Query: 261 LGIILGSQFVKRLAGRMDERRIFQIGLQLAFITTAAILMIVLAHGPFFALFISVFLFAAS 320 L II GS + R G + E+ + +I + A I TA +L + + HGP L IS+F++ + Sbjct: 264 LAIISGSFIIGRFGGIIHEKSLLRIAVITAMIATAVLLTMTMIHGPLATLVISIFIYMIT 323 Query: 321 IGIIGPVSFTLAMESQGHIAGSAAAILGTLQFALGAVTSPLVGLGGDNSAIPFGITIFMT 380 IG++ +FTLAME QGH AGSA+A+LG L LG++ SPLVG+ + +A+P G +F+T Sbjct: 324 IGMVLTSTFTLAMEKQGHRAGSASALLGMLPLLLGSIVSPLVGI-NETTAVPMGAIMFVT 382 Query: 381 SILAVIAFVFLVKK 394 +++ +AF L K+ Sbjct: 383 AVIGSLAFFGLTKE 396 >A7Z1W6.1 A7Z1W6_BACA2 YdgK Length = 403 Score = 301 bits (771), Expect = 8e-80, Method: Compositional matrix adjust. Identities = 164/379 (43%), Positives = 230/379 (60%), Gaps = 3/379 (0%) Query: 23 GPFTVDMYLSSLPQLMSYFGTNTSMIXXXXXXXXXXXXXXXXXXXPISDVHGRRKPLLIS 82 GP +DMYL S P++ S++ P+SD GRRKPLL+ Sbjct: 24 GPLNIDMYLPSFPEIAEDLSARASLVQLSLTACLIGLTIGQVVVGPLSDAKGRRKPLLLC 83 Query: 83 MILYLLTSLACAFSPNIGLFIALRFIQGFVASAGLVISRAIVRDRYNGIEMTKFMSLLTM 142 + L+ L SL CA +PNI + RF+QGF ASAGLV+SRAIVRD + G E++KF SLL + Sbjct: 84 IFLFALFSLFCALAPNITTLVIARFLQGFTASAGLVLSRAIVRDVFTGRELSKFFSLLMV 143 Query: 143 ISNIAPLISPTAGSTVT--TFTSWXXXXXXXXXXXXXXXXXXXWGIKESLPAERRISSNF 200 I+ +AP+++P G + F SW + E+LP E+RI S+ Sbjct: 144 ITAVAPMVAPMTGGAILLLPFASWHTIFLFLTFIGFLLVLIIALKLTETLPPEKRIPSSI 203 Query: 201 REVLRNYDSLLRDRSFMGYALVNGILFSGVFAYVAGTPFIYQNMYGISPQLFSILFALNG 260 +R SLL+DRSF+GYAL G + G FAYV+GTPF+YQ++YG+SPQ+FS+LF +NG Sbjct: 204 GNSVRTMGSLLKDRSFIGYALTVGFIHGGSFAYVSGTPFVYQDIYGVSPQVFSVLFGING 263 Query: 261 LGIILGSQFVKRLAGRMDERRIFQIGLQLAFITTAAILMIVLAHGPFFALFISVFLFAAS 320 L II GS + R G + E+ + +IG+ A TA +L++ + HGP AL IS+F++ + Sbjct: 264 LAIITGSFIIGRFGGIIHEKSLLRIGVITAMTATAVLLVMTIIHGPLAALVISIFIYMIT 323 Query: 321 IGIIGPVSFTLAMESQGHIAGSAAAILGTLQFALGAVTSPLVGLGGDNSAIPFGITIFMT 380 IG+ +FTLAME QGH AGSA+A+LG L LG++ SPLVG+ + +A+P G +F T Sbjct: 324 IGMTLTSTFTLAMEKQGHRAGSASALLGMLPLLLGSIVSPLVGM-NEATAVPMGAIMFTT 382 Query: 381 SILAVIAFVFLVKKPTLAS 399 S++ IAF L KP A Sbjct: 383 SVIGSIAFFALTTKPEKAK 401 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.328 0.142 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,808,321,610 Number of extensions: 67047854 Number of successful extensions: 297630 Number of sequences better than 1.0e-05: 3099 Number of HSP's gapped: 293648 Number of HSP's successfully gapped: 3110 Length of query: 400 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 262 Effective length of database: 1,760,596,133 Effective search space: 461276186846 Effective search space used: 461276186846 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)