BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0435.0006.p (274 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C4C5S3.1 C4C5S3_9FUSO Uncharacterized protein similar to gamma-c... 295 2e-78 C1Q958.1 C1Q958_9SPIR Uncharacterized protein similar to gamma-c... 287 7e-76 A6L9W8.1 A6L9W8_PARD8 Carboxymuconolactone decarboxylase 285 3e-75 Q8TTM1.1 Q8TTM1_METAC 4-carboxymuconolactone decarboxylase 281 4e-74 C4IK84.1 C4IK84_CLOBU 4-carboxymuconolactone decarboxylase 281 5e-74 >C4C5S3.1 C4C5S3_9FUSO Uncharacterized protein similar to gamma-carboxymuconolactone decarboxylase subunit Length = 248 Score = 295 bits (756), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 132/247 (53%), Positives = 180/247 (72%) Query: 24 MNRIEKSKETYKRLFGDGVPTAYATDPDLQEILSRFIFGEVFDQGKLDDKQRELITLVVL 83 M+R +E K+LFGD + + DP+ EIL++F+FGEV++ GKL DK+R LITL L Sbjct: 1 MDRELLYREKMKQLFGDADSSLFTADPEFAEILNKFVFGEVYNHGKLTDKERILITLCAL 60 Query: 84 AANQTLPQLKAHANAALNVGLTPVEIKEAIYQCAPYLGFPKTLNAANKVNEVFKARNIEL 143 A K + A LN GL+P +IKEA+YQCAPY GFPK L A +K ++VF+ I L Sbjct: 61 TAGGIFKNFKRYIKAGLNTGLSPEDIKEALYQCAPYTGFPKALKAIHKADKVFRKAGISL 120 Query: 144 PLESQTQVTDDTRLEKGLAVQTEIFGDNVAKMHGSAPDNQKHIQDYLSAFCFGDFYTRNG 203 P++S + ++ R +GL VQ IFG+ + +MH +AP+NQKHIQ+YLSA CFGDFYTR+G Sbjct: 121 PVKSMKKTDENNRFNEGLKVQETIFGERIRRMHENAPENQKHIQNYLSAMCFGDFYTRDG 180 Query: 204 LDLQTRELLTLCIISALGGAEGQVKAHVQGNKNVGNDKETLIAAITHCLPYMGFPRTLNA 263 LD++ RELLTLCI+SALGG E Q+++H++GN + GNDK+ +I A+T C+PY+GFPRTLNA Sbjct: 181 LDIKMRELLTLCILSALGGCENQLRSHIEGNLSAGNDKDIMIEAVTQCMPYIGFPRTLNA 240 Query: 264 IANINEV 270 + INE Sbjct: 241 LGCINET 247 >C1Q958.1 C1Q958_9SPIR Uncharacterized protein similar to gamma-carboxymuconolactone decarboxylase subunit Length = 281 Score = 287 bits (735), Expect = 7e-76, Method: Compositional matrix adjust. Identities = 133/243 (54%), Positives = 172/243 (70%) Query: 28 EKSKETYKRLFGDGVPTAYATDPDLQEILSRFIFGEVFDQGKLDDKQRELITLVVLAANQ 87 EK+ Y LF ++ D +L+ I + F++GEV++ G LD K REL+TLV L A+Q Sbjct: 38 EKANSKYNELFKREAKSSKPNDEELESIFNNFLYGEVYNHGSLDAKLRELVTLVSLTASQ 97 Query: 88 TLPQLKAHANAALNVGLTPVEIKEAIYQCAPYLGFPKTLNAANKVNEVFKARNIELPLES 147 +K H ALNVG+TP+EIKEA+YQC+PY+GFP+ A K NEVFKA+NI LP+ES Sbjct: 98 GTDFIKDHVEIALNVGVTPIEIKEALYQCSPYVGFPRVFAALEKANEVFKAKNISLPIES 157 Query: 148 QTQVTDDTRLEKGLAVQTEIFGDNVAKMHGSAPDNQKHIQDYLSAFCFGDFYTRNGLDLQ 207 Q+ VT++TR +KGL Q IFG+ + H +A NQKHIQ+YLSA CFGDFYTR GLDL+ Sbjct: 158 QSTVTEETRFDKGLETQVSIFGNAILTAHSNAAPNQKHIQNYLSANCFGDFYTRTGLDLK 217 Query: 208 TRELLTLCIISALGGAEGQVKAHVQGNKNVGNDKETLIAAITHCLPYMGFPRTLNAIANI 267 TRELLT +I +LGGAE Q AH N +GN K+ +I A+T CLPY+G+PRTLNAI I Sbjct: 218 TRELLTFIMIISLGGAEPQATAHANANIAMGNTKDMMIEAVTQCLPYIGYPRTLNAITVI 277 Query: 268 NEV 270 N + Sbjct: 278 NNI 280 Score = 77.0 bits (188), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 42/115 (36%), Positives = 66/115 (57%), Gaps = 5/115 (4%) Query: 26 RIEKSKETYKRLFGDGVPTAYA----TDPDLQEILSRFIFGEVFDQGKLDDKQRELITLV 81 R +K ET +FG+ + TA++ +Q LS FG+ + + LD K REL+T + Sbjct: 166 RFDKGLETQVSIFGNAILTAHSNAAPNQKHIQNYLSANCFGDFYTRTGLDLKTRELLTFI 225 Query: 82 -VLAANQTLPQLKAHANAALNVGLTPVEIKEAIYQCAPYLGFPKTLNAANKVNEV 135 +++ PQ AHANA + +G T + EA+ QC PY+G+P+TLNA +N + Sbjct: 226 MIISLGGAEPQATAHANANIAMGNTKDMMIEAVTQCLPYIGYPRTLNAITVINNI 280 >A6L9W8.1 A6L9W8_PARD8 Carboxymuconolactone decarboxylase Length = 429 Score = 285 bits (729), Expect = 3e-75, Method: Compositional matrix adjust. Identities = 136/247 (55%), Positives = 176/247 (71%), Gaps = 2/247 (0%) Query: 24 MNRIEKSKETYKRLFGDGVPTAYATDPDLQEILSRFIFGEVFDQGKLDDKQRELITLVVL 83 M+RIE+ K+ Y LFG T TDP++ +IL +FIFGEVF G LD K RELIT VL Sbjct: 75 MDRIERCKKNYTELFGGEALTGQGTDPEMMDILQKFIFGEVFRTGDLDKKTRELITCTVL 134 Query: 84 AANQTLPQLKAHANAALNVGLTPVEIKEAIYQCAPYLGFPKTLNAANKVNEVFKARNIEL 143 A QTLPQL AHA AALNVG+TP+E++EAIY CAP++GFPKTLNA N +NEVFK + I L Sbjct: 135 ATMQTLPQLNAHAKAALNVGVTPIELREAIYLCAPFIGFPKTLNALNTINEVFKQQGIAL 194 Query: 144 PLESQTQVTDDTRLEKGLAVQTEIFGDNVAKMHGSAPDNQ-KHIQDYLSAFCFGDFYTRN 202 PLE Q VT++ R EKG A+Q+ ++G+ + + + P N ++ +L+ FCFGD YTRN Sbjct: 195 PLERQATVTEEDRHEKGQAIQSRLYGEGIKEAMRNVPGNMGPEVERFLTEFCFGDIYTRN 254 Query: 203 GLDLQTRELLTLCIISALGGAEGQVKAHVQGNKNVGNDKETLIAAITHCLPYMGFPRTLN 262 GLDL+TRELL CI++ L AE Q+ +H++GN GN KETL AA+ CLPY+GFP + Sbjct: 255 GLDLKTRELLAYCILTTL-EAESQLHSHLEGNLLAGNSKETLTAAVIQCLPYIGFPSAIK 313 Query: 263 AIANINE 269 A+ I E Sbjct: 314 ALKIIKE 320 Score = 74.7 bits (182), Expect = 7e-12, Method: Compositional matrix adjust. Identities = 47/124 (37%), Positives = 66/124 (53%), Gaps = 6/124 (4%) Query: 147 SQTQVTDDTRLEKGLAVQTEIFGDNVAKMHGSAPDNQKHIQDYLSAFCFGDFYTRNGLDL 206 S+T+ D R+E+ TE+FG G+ P+ + D L F FG+ + LD Sbjct: 69 SKTEQNMD-RIERCKKNYTELFGGEALTGQGTDPE----MMDILQKFIFGEVFRTGDLDK 123 Query: 207 QTRELLTLCIISALGGAEGQVKAHVQGNKNVGNDKETLIAAITHCLPYMGFPRTLNAIAN 266 +TREL+T C + A Q+ AH + NVG L AI C P++GFP+TLNA+ Sbjct: 124 KTRELIT-CTVLATMQTLPQLNAHAKAALNVGVTPIELREAIYLCAPFIGFPKTLNALNT 182 Query: 267 INEV 270 INEV Sbjct: 183 INEV 186 >Q8TTM1.1 Q8TTM1_METAC 4-carboxymuconolactone decarboxylase Length = 250 Score = 281 bits (720), Expect = 4e-74, Method: Compositional matrix adjust. Identities = 135/250 (54%), Positives = 173/250 (69%) Query: 24 MNRIEKSKETYKRLFGDGVPTAYATDPDLQEILSRFIFGEVFDQGKLDDKQRELITLVVL 83 M E + +K LF D T TDP+L E+ F F EV G LD K R ++ L L Sbjct: 1 MRISETANRNHKELFPDHKSTLKVTDPELIEVFDNFAFDEVISYGNLDTKTRMMVILGSL 60 Query: 84 AANQTLPQLKAHANAALNVGLTPVEIKEAIYQCAPYLGFPKTLNAANKVNEVFKARNIEL 143 A+QTL + K ALNVG+TP+E+KE +YQ PY+G K + + NE+ ++R IEL Sbjct: 61 IASQTLSEYKVMLGGALNVGVTPIEVKEIVYQSVPYVGIAKIFDFIHATNEILESRGIEL 120 Query: 144 PLESQTQVTDDTRLEKGLAVQTEIFGDNVAKMHGSAPDNQKHIQDYLSAFCFGDFYTRNG 203 PLE Q+ + +TR EKGL VQ EIFGD + KM+ +AP+NQ HIQ YLSA CFGD+YTR G Sbjct: 121 PLEGQSTTSPETRFEKGLEVQKEIFGDIIDKMYEAAPENQIHIQKYLSANCFGDYYTRKG 180 Query: 204 LDLQTRELLTLCIISALGGAEGQVKAHVQGNKNVGNDKETLIAAITHCLPYMGFPRTLNA 263 LD++TRELLT +I ALGG E Q+K H+QGN NVGNDK+TL++ +T LPY+G+PRTLNA Sbjct: 181 LDVKTRELLTFSMILALGGCEPQLKGHIQGNLNVGNDKKTLLSTVTQLLPYIGYPRTLNA 240 Query: 264 IANINEVIPE 273 IA +NEVIPE Sbjct: 241 IACLNEVIPE 250 >C4IK84.1 C4IK84_CLOBU 4-carboxymuconolactone decarboxylase Length = 250 Score = 281 bits (718), Expect = 5e-74, Method: Compositional matrix adjust. Identities = 128/249 (51%), Positives = 175/249 (70%), Gaps = 1/249 (0%) Query: 24 MNRIEKSKETYKRLFGDGVPTAYATDPDLQEILSRFIFGEVFDQGKLDDKQRELITLVVL 83 M+RI+ S++ K+L+GD +D +L EI+ RFI+GEV+ G L+ K RELI + V Sbjct: 1 MDRIKSSEKIQKQLWGDKNSELMTSDSELYEIMKRFIYGEVWQHGNLEPKIRELIIIAVN 60 Query: 84 AANQTLPQLKAHANAALNVGLTPVEIKEAIYQCAPYLGFPKTLNAANKVNEVFKARNIEL 143 N + Q H AALN+G++PVEIKE +YQCAPY+GF K NA N++F+ + ++L Sbjct: 61 ITNNNMEQCAEHVEAALNIGISPVEIKETLYQCAPYVGFSKVQNAIVVTNQIFEEKGVKL 120 Query: 144 PLESQTQVTDDTRLEKGLAVQTEIFG-DNVAKMHGSAPDNQKHIQDYLSAFCFGDFYTRN 202 PLE+Q+ T+D R+ KG+ VQ IFG +N+ + +APDN KHIQDYLS +CFGD RN Sbjct: 121 PLENQSTTTEDNRIAKGIEVQKSIFGSENIDNLRANAPDNLKHIQDYLSGYCFGDICGRN 180 Query: 203 GLDLQTRELLTLCIISALGGAEGQVKAHVQGNKNVGNDKETLIAAITHCLPYMGFPRTLN 262 LD++ REL+T II+ LGG E Q++AH+ GN +GNDKE L+ IT C+PY+GFPRTLN Sbjct: 181 SLDIKIRELITFSIIATLGGCENQLRAHIGGNAAIGNDKEILLNVITQCMPYIGFPRTLN 240 Query: 263 AIANINEVI 271 AI INEV+ Sbjct: 241 AITCINEVL 249 Score = 76.3 bits (186), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 46/119 (38%), Positives = 63/119 (52%), Gaps = 6/119 (5%) Query: 25 NRIEKSKETYKRLFG-DGVPTAYATDPD----LQEILSRFIFGEVFDQGKLDDKQRELIT 79 NRI K E K +FG + + A PD +Q+ LS + FG++ + LD K RELIT Sbjct: 132 NRIAKGIEVQKSIFGSENIDNLRANAPDNLKHIQDYLSGYCFGDICGRNSLDIKIRELIT 191 Query: 80 LVVLAA-NQTLPQLKAHANAALNVGLTPVEIKEAIYQCAPYLGFPKTLNAANKVNEVFK 137 ++A QL+AH +G + I QC PY+GFP+TLNA +NEV K Sbjct: 192 FSIIATLGGCENQLRAHIGGNAAIGNDKEILLNVITQCMPYIGFPRTLNAITCINEVLK 250 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,548,633,770 Number of extensions: 62303576 Number of successful extensions: 140185 Number of sequences better than 1.0e-05: 582 Number of HSP's gapped: 139642 Number of HSP's successfully gapped: 892 Length of query: 274 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 140 Effective length of database: 1,798,280,193 Effective search space: 251759227020 Effective search space used: 251759227020 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)