BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0435.0001.p (181 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DGV0.1 B1DGV0_9BACL Transcriptional regulator, TetR family 212 9e-54 B3KEK7.1 B3KEK7_9BACI Regulatory protein TetR 211 3e-53 A9VHA0.1 A9VHA0_BACWK Transcriptional regulator, TetR family 202 1e-50 C1PXQ9.1 C1PXQ9_ALIAC Transcriptional regulator, TetR family 161 3e-38 B7IL55.1 B7IL55_BACC2 Transcriptional regulator, TetR family 154 3e-36 >B1DGV0.1 B1DGV0_9BACL Transcriptional regulator, TetR family Length = 185 Score = 212 bits (540), Expect = 9e-54, Method: Compositional matrix adjust. Identities = 103/185 (55%), Positives = 138/185 (74%), Gaps = 4/185 (2%) Query: 1 MMKVDRRVLKSQEAIKKAIIELMSEKSFDDITIRDISDRANVNRGTIYLHYMDKYDLLDK 60 M KVDRR++K+QE++K A+I+LMSEKSFD+ITI+DI+DRAN+NRGTIYLHY DKYDLLDK Sbjct: 1 MPKVDRRIVKTQESLKHAVIDLMSEKSFDEITIQDIADRANLNRGTIYLHYQDKYDLLDK 60 Query: 61 LIEEHISNLRELCHSASEMTFQEGNYVWFEYFAENHLFFTTMLNTKSA----AYFRSRFL 116 LIE H++ L E+ A EM + + +F+YF EN LFF+TML +K A + FR+R L Sbjct: 61 LIESHLNELGEMDKWACEMDWIDALVPYFKYFEENFLFFSTMLASKEAKGAPSSFRTRLL 120 Query: 117 DLVVQEYKPEVDVTEGKNQGLSEEVFLQFFGAAVVGAVEWWFRNGMLVPPRVMAEQTGIL 176 + ++ +K E+D +N LS+EV LQ+ G A VG +EWW RNGM PP VMA+Q G+L Sbjct: 121 NSFMEGFKGEIDRESERNTDLSDEVMLQYAGTAYVGVIEWWIRNGMPHPPEVMAKQVGVL 180 Query: 177 LDRNF 181 L+R+ Sbjct: 181 LERSL 185 >B3KEK7.1 B3KEK7_9BACI Regulatory protein TetR Length = 182 Score = 211 bits (536), Expect = 3e-53, Method: Compositional matrix adjust. Identities = 99/182 (54%), Positives = 137/182 (75%), Gaps = 1/182 (0%) Query: 1 MMKVDRRVLKSQEAIKKAIIELMSEKSFDDITIRDISDRANVNRGTIYLHYMDKYDLLDK 60 M KVDRR+LK+QEA++KA+IELM++K+FDDITI+DI+D+AN+NRGTIYLHY DKYDLL++ Sbjct: 1 MAKVDRRILKTQEALRKAVIELMADKNFDDITIQDIADQANLNRGTIYLHYQDKYDLLNQ 60 Query: 61 LIEEHISNLRELCHSASEMTFQEGNYVWFEYFAENHLFFTTMLNTKSA-AYFRSRFLDLV 119 ++E HI L+E+ A EM + + +FEYF +N++ F+TML +K A + FR+R L Sbjct: 61 IMEAHIQELKEMDTWACEMEWADALVPYFEYFEKNYVLFSTMLASKGAPSSFRTRLLTSF 120 Query: 120 VQEYKPEVDVTEGKNQGLSEEVFLQFFGAAVVGAVEWWFRNGMLVPPRVMAEQTGILLDR 179 ++ +K E+D GKN L E+V LQ+ G A VG +EWW RNGM P+VMA+Q G LLDR Sbjct: 121 MEGFKGEIDKESGKNAELHEDVMLQYAGTAYVGVIEWWIRNGMPYSPQVMAKQVGTLLDR 180 Query: 180 NF 181 + Sbjct: 181 SL 182 >A9VHA0.1 A9VHA0_BACWK Transcriptional regulator, TetR family Length = 181 Score = 202 bits (514), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 92/181 (50%), Positives = 129/181 (71%) Query: 1 MMKVDRRVLKSQEAIKKAIIELMSEKSFDDITIRDISDRANVNRGTIYLHYMDKYDLLDK 60 M KVDRR++KS+EAIK A IELM+EK FD IT++DI A+V T YLHY+DK+DLLDK Sbjct: 1 MEKVDRRIIKSKEAIKNAFIELMAEKGFDKITVKDICSGADVGNRTFYLHYLDKFDLLDK 60 Query: 61 LIEEHISNLRELCHSASEMTFQEGNYVWFEYFAENHLFFTTMLNTKSAAYFRSRFLDLVV 120 L+ E I L+ LC +++F+E WFE +++ FF+TML K A+ FR F D ++ Sbjct: 61 LVIERIEALKTLCAPLHDLSFREACIAWFENMEQHYFFFSTMLAGKGASAFRKHFFDYII 120 Query: 121 QEYKPEVDVTEGKNQGLSEEVFLQFFGAAVVGAVEWWFRNGMLVPPRVMAEQTGILLDRN 180 ++ K +VD+ EG N+G SE++ + FFG+A+VG VE +F G+ PP ++AEQ G+LLDRN Sbjct: 121 EQIKDDVDIKEGINKGFSEDMIITFFGSAIVGVVETYFMKGLPDPPEIVAEQLGMLLDRN 180 Query: 181 F 181 F Sbjct: 181 F 181 >C1PXQ9.1 C1PXQ9_ALIAC Transcriptional regulator, TetR family Length = 200 Score = 161 bits (407), Expect = 3e-38, Method: Compositional matrix adjust. Identities = 76/175 (43%), Positives = 112/175 (64%) Query: 4 VDRRVLKSQEAIKKAIIELMSEKSFDDITIRDISDRANVNRGTIYLHYMDKYDLLDKLIE 63 +DRRV KS++AI+ A + LM EK FD IT++DI++RANV+R T YLH++DKYDLLD+++E Sbjct: 1 MDRRVQKSRQAIRDAFVALMKEKDFDHITVQDITERANVSRKTFYLHFLDKYDLLDRVME 60 Query: 64 EHISNLRELCHSASEMTFQEGNYVWFEYFAENHLFFTTMLNTKSAAYFRSRFLDLVVQEY 123 + I ++ E SE+ + F+Y A+ + FF TML A YFR R+++ + Sbjct: 61 DAIRDMDEFGQCVSELDWVPATEQCFQYLADRYDFFGTMLTQAGAPYFRRRYVESCKASF 120 Query: 124 KPEVDVTEGKNQGLSEEVFLQFFGAAVVGAVEWWFRNGMLVPPRVMAEQTGILLD 178 E+ G++ E+ LQF A VG VEWW + GM PPRVMA++ G +L+ Sbjct: 121 TREIRRVVGRDPLPEEDAILQFVVNAYVGTVEWWLQEGMPYPPRVMADRIGRMLE 175 >B7IL55.1 B7IL55_BACC2 Transcriptional regulator, TetR family Length = 181 Score = 154 bits (389), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 67/180 (37%), Positives = 117/180 (65%) Query: 1 MMKVDRRVLKSQEAIKKAIIELMSEKSFDDITIRDISDRANVNRGTIYLHYMDKYDLLDK 60 M K+DRR++KSQ AI+ A IE++ FD+IT+++I+++AN+ R T YLHY+DKY+LLDK Sbjct: 1 MEKIDRRIIKSQNAIQSAFIEMLITDGFDEITVKNITEKANIGRKTFYLHYLDKYNLLDK 60 Query: 61 LIEEHISNLRELCHSASEMTFQEGNYVWFEYFAENHLFFTTMLNTKSAAYFRSRFLDLVV 120 ++++H++ LRE+C + EG ++WFEYF E+ FF ++ + S F+ +FL ++ Sbjct: 61 IVDDHLNQLREICDIKQTKGYIEGTFIWFEYFKEHKPFFASLFRSNSTLSFQKKFLTFIM 120 Query: 121 QEYKPEVDVTEGKNQGLSEEVFLQFFGAAVVGAVEWWFRNGMLVPPRVMAEQTGILLDRN 180 E + +++ N+ + + + L+F G AV+G +E + + + +A Q G L+ RN Sbjct: 121 SELEKKLNANTSVNKNIDKHIVLKFLGTAVMGILESYVLDEIDDDIEYIATQVGELMRRN 180 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.137 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,008,255,712 Number of extensions: 39377704 Number of successful extensions: 130925 Number of sequences better than 1.0e-05: 917 Number of HSP's gapped: 130606 Number of HSP's successfully gapped: 918 Length of query: 181 Length of database: 3,060,696,203 Length adjustment: 127 Effective length of query: 54 Effective length of database: 1,864,227,298 Effective search space: 100668274092 Effective search space used: 100668274092 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 127 (53.5 bits)